تاثیر پرایمینگ بذر کینوا رقم گیزا یک (Chenopodium quinoa L. var. Giza 1) با اسید آسکوربیک در افزایش تحمل به شوری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه زراعت، دانشکده کشاورزی دانشگاه شهر کرد

2 عضو هیئت علمی دانشگاه شهرکرد

10.22124/jms.2023.7676

چکیده

برای ارزیابی تاثیر پرایمینگ بذر با اسید آسکوربیک بر افزایش تحمل به شوری گیاه کینوا رقم گیزا 1 در طول دوره رشد رویشی، تحقیق حاضر در قالب آزمایش فاکتوریل (اسید آسکوربیک در سه سطح صفر، 20 و 60 میلی­مولار و NaCl در دو سطح صفر و 400 میلی­مولار) و بر پایه طرح کاملاً تصادفی انجام شد. صفات اندازه گیری­شده در این آزمایش شامل وزن­تر و خشک، غلظت رنگیزه­های فتوسنتزی، پراکسید هیدروژن (H2O2) و مالون دی آلدئید (MDA) و نیز فعالیت آنزیم­های آنتی­اکسیدان بود. آنالیز داده­ها نشان داد تیمار NaCl (400 میلی­مولار) باعث کاهش معنی­دار وزن­خشک (4/51 درصد) و وزن­تر (6/56 درصد)، غلظت کلروفیل کل (8/55 درصد) شد. علاوه براین، شوری باعث افزایش محتوای MDA (3/2 برابر)، H2O2 (5/2 برابر) و فعالیت آنزیم­های آنتی­اکسیدان (کاتالاز، آسکوربات پراکسیداز و گایاکول پراکسیداز) شد. با این­وجود، پرایمینگ بذر با اسید آسکوربیک در بهترین غلظت (20 میلی­مولار)، با افزایش وزن­تر (7/44 درصد)، وزن­خشک (4/51 درصد) و حفاظت از انسجام غشاهای سلولی و فعالیت فتوسنتزی، تاثیر نامطلوب تنش شوری را در این رقم تخفیف داد. علاوه براین، استفاده از اسید آسکوربیک با تغییر در فعالیت آنزیم­های آنتی­اکسیدان و کاهش محتوای  H2O2از اثرات مخرب تنش اکسیداتیو در این رقم تحت تنش شوری کاست. به­طورکلی می­توان نتیجه گرفت پرایمینگ بذر این رقم با غلظت 20 میلی­مولار اسید آسکوربیک می­تواند بر کشت موفقیت­آمیز آن در درجات بالای شوری موثر باشد.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of seed priming of Chenopodium quinoa L. var. Giza 1 with ascorbic acid on increasing salt tolerance

نویسندگان [English]

  • Manizheh Jahantighi 1
  • Parto Roshandel 2
1 Agronomy.academy agricultural.city. kord charmahal bakhtiyari.iran
2 Agronomy Dept. Faculty of Agriculture , Shahrekord University, Shahrekord, Iran
چکیده [English]

To evaluate the effect of seed priming with ascorbic acid to increase salt tolerance in Chenopodium quinoa var. Giza 1 during the vegetative growth period, the current research was conducted in the form of a factorial experiment (ascorbic acid at three levels of 0, 20 and 60 mM and NaCl at two levels of 0 and 400 mM) and based on a completely randomized design. The traits measured in the experiment were fresh and dry weight, concentration of photosynthetic pigments, hydrogen peroxide (H2O2) and malondialdehyde, and the activity of antioxidant enzymes. Data analysis showed salinity resulted in a significant decrease in the amount of dry weight (51.4%), fresh weight (65.6%) and total chlorophyll (55.8%). In addition, salinity increased the content of malondialdehyde (2.3 folds), hydrogen peroxide (2.5 folds) and the activity of antioxidant enzymes (catalase, ascorbate peroxidase and guaiacol peroxidase). However, seed priming with ascorbic acid at the best level (20 mM) alleviated the adverse effects of NaCl in this variety by increasing the fresh weight (44.7%), dry weight (51.4%), and protecting membrane integrity and photosynthesis activity. Also, the use of ascorbic acid by changing the activity of antioxidant enzymes and reducing the content of hydrogen peroxide caused a reduction in oxidative stress in in this variety. It can be concluded that seed priming of this variety with 20 mM ascorbic acid can be effective on its successful cultivation in high salinity levels.

کلیدواژه‌ها [English]

  • Halophyte
  • Oxidative stress
  • Quinoa
  • Seed pretreatment
  • Vitamin C
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