اثر پرایمینگ بذر با اسید سالیسیلیک بر شاخص‌های جوانه‌زنی و رشد گیاهچه چای ترش توده بومی سیستان تحت تنش خشکی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد رشته زراعت دانشکده‌ کشاورزی، دانشگاه شهرکرد

2 استادیار-گروه مهندسی زراعت-دانشکده کشاورزی-دانشگاه شهرکرد

چکیده

به­منظور بررسی اثر پرایمینگ بذر با اسید سالیسیلیک بر شاخص­های جوانه­زنی و رشد گیاهچه چای ترش تحت تنش خشکی، آزمایشی به­صورت فاکتوریل در قالب طرح بلوک کامل تصادفی با چهار تکرار انجام شد. عامل اول شامل سطوح مختلف تنش خشکی در چهار سطح (0، 2- ، 4- و 6- بار) و فاکتور دوم شامل سطوح مختلف اسید سالیسیلیک در چهار سطح (0، 5/0، 1 و 5/1 میلی­مولار) بود. صفات اندازه­گیری شده شامل درصد جوانه­زنی، سرعت جوانه­زنی، میانگین زمان جوانه­زنی، ضریب سرعت جوانه­زنی، طول ریشه­چه، طول ساقه­چه و وزن خشک گیاهچه بود. نتایج نشان داد که در بین سطوح اسید سالیسیلیک پرایمینگ با سطح 1 میلی مولار به ترتیب سبب افزایش 42 و 14 درصدی سرعت جوانه­زنی و ضریب سرعت جوانه­زنی در مقایسه با تیمار شاهد شد. غلظت 5/0 میلی مولار اسید سالیسیلیک نیز به ترتیب باعث افزایش 17، 18 و 27 درصدی طول ریشه­چه، طول ساقه­چه و وزن خشک گیاهچه در مقایسه با تیمار شاهد گردید. پرایمینگ میانگین زمان جوانه­زنی را به­طور معنی­داری در شرایط تنش خشکی کاهش داد. تنش خشکی نیز سبب کاهش کلیه صفات مورد بررسی به جز متوسط زمان جوانه­زنی گردید. در مجموع نتایج نشان داد که پیش تیمار بذر چای ترش با اسید سالیسیلیک در شرایط تنش خشکی می­تواند تا حدی موجب افزایش مقاومت این گیاه و بهبود جوانه­زنی آن در مراحل اولیه جوانه­زنی و رشد گیاه گردد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of seed priming with salicylic acid on seed germination and seedling growth of Hibiscus sabdariffa under drought stress

نویسندگان [English]

  • Elahe Ahmadpoor Dehkordi 1
  • Abdolrazagh Danesh Shahraki 2
  • Parisa Khosravi Lamjiri 1
چکیده [English]

In order to evaluate the effect of seed priming with salicylic acid on seed germination and seedling growth of Hibiscus sabdariffa under drought stress a factorial experiment was conducted in a randomized complete block design with four replications. First factor was included different levels of drought stress (0, -2, -4 and -6 bar) and the second factor was different levels of seed priming with salicylic acid at four levels (0, 0.5, 1 and 1.5 mM). Results showed that among different levels of seed priming, priming with 1 mM salicylic acid increased germination rate and coefficient of velocity of germination, compared with control by 42 and 14%, respectively. Salicylic acid concentrations of 0.5 mM enhanced, root length, shoot length and seedling dry weight compared with the control treatment, by 17, 18 and 27%, respectively. At drought conditions, priming reduced the mean germination time, significantly. Moreover drought stress decreased all measured traits except mean germination time. Overall, results showed that seed priming with salicylic acid in stress conditions improved roselle germination.

کلیدواژه‌ها [English]

  • Drought stress
  • Germination rate
  • Medicinal plant
  • Seed enhancement
  • Seed treatment
Agrawal, P.K. and Dadlani, M. 1995. Techniques in seed science and technology. Second Edition. South Asian Publishers.
Askari, A. and solangi, M. S. 1995. Autecological studies of exotic plant Hibiscus sabdariffa L. (Roselle), a multipurpose plant, for is introduction and cultur. Pakistan Journal of Scientific and Industrial Reserch. 38: 17-21. (Journal)
Ansari, O. and Sharif Zadeh, F. 2010. Osmo and hydro priming mediated germination improvement under cold stress conditions in mountain rye (Secale montanum). Cercetări Agronomice in Moldova. 3: 53-62. (Journal)
Ashraf, M. and Foolad, M. R. 2007. Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany. 59:206-216. (Journal)
Baraani Dastjerdi, M., Rafieiolhossaini, M. and Danesh-Shahraki, A.R. 2014. Effects of drought stress and foliar application of zinc and manganese on seed quality of red bean during the accelerated aging test. Crop Production. 7:77-95. (In Persian) )Journal)
Ellis, R. A. and Roberts, E. H. 1981. The quantification of ageing and survival in orthodox seeds. Seed Science and Technology. 9:373-409. (Journal)
El-Tayeb, M. A. 2005. Response of barley grains to the interactive effect of salinity and salicylic acid. Plant Growth Regulation. 45:215-225. (Journal)
Farooq, M., Basra, S. M. A., Wahid, A. and Khan, M. B. 2006. Rice seed invigoration by hormonal and vitamin priming. Seed Science and Technology. 34:775-780. (Journal)
Farooq, M., Basra, S. M. A., Tabassum, R. and Ahmed, N. 2006. Evaluation of seed vigor enhancement techniques on physiological and biochemical basis in coarse rice (Oryza sativa L.). Seed Science and Technology. 34:741-750. (Journal)
Halmer, P. 2004. Methods to improve seed performance in the field. In: Benech-Arnold, R.L. And Sanchez, R.A. (Eds.) Handbook of Seed Physiology; Application to Agriculture. The Haworth Press, New York. pp: 125-165.
Hanan, E.D. 2007. Influence of salicylic acid on stress tolerance during seed germination of Triticum aestivum and Hordeum vulgare. Biological Research. 1:40-48. (Journal)
Ikic, I., Maric evic, M., Tomasovic, S., Gunjaca, J., Atovic, Z. S. and Arcevic, H. S. 2012. The effect of germination temperature on seed dormancy in Croatian-grown winter wheats. Euphytica. 188: 25- 34. (Journal)
ISTA (International Seed Testing Association). 2009. International Rules for Seed Testing. International Seed Testing Association, Bassersdorf, Switzerland.
Kabiri, R., Farahbakhsh, H. and Nasibi, F. 2012. Effect of drought stress and its interaction with salicylic acid on black cumin (Nigella sativa) germination and seedling growth. World Applied Sciences Journal. 18: 520-527. (In Persian) )Journal)

Kafi, M., Nezami, A., Hosaini, H. and Masomi, A. 2005. Physiological effects of drought stress by polyethylene glycol on germination of lentil (Lens culinaris Medik.) genotypes.  Journal of Iranian Field Crop Research. 3: 69-80. (In Persian) )Journal)

Karta, k. k. and Bekele. A. 2012. Influence of seed priming on germination and vigor traits of Vicia villosa. African Journal of Agricultural Recearch. 7 (21): 3202- 3208. (Journal)
Khan, W., Prithiviraj, B. and Smith, D. L. 2003. Photosynthetic response of corn and soybean to foliar application of salicylates. Journal of Plant Physiology. 160: 485-492. (Journal)
Mayer, A.M. and Polijakoff-Mayber, A. 1989. The Germination of Seeds (4th Ed.). Pergamon Press. Oxford.
Mazaheri Tirani, M. and Kalantari, Kh. 2006. Effects of the role of salicylic acid, drought stress, ethylene and interaction of three factors on seed germination of Brassica napus. Iranian Journal of Biology. 19: 408-418. (In Persian) )Journal)
Metwally, A.I., Finkemeier, M., Georgi, K. and Dietz, J. 2003. Salicylic acid alleviates the cadmium toxicity in barley seedlings. Plant Physiology. 132: 272- 281. (Journal)
 
Michel, B.E. and Kaufmann, M.R. 1937. The osmotic potential of Polyethylene Glycol 6000. Plant Physiology. 51: 914- 916. (Journal)
Mohammed, R., Fernandez, J., Pineda, M. and Aguilar, M. 2007. Roselle (Hibiscus sabdariffa) seed oil is a rich source of gamma-tocopherol. Journal of Food Science. 72: 207– 211. (Journal)
Prasongwatana, V., Woottisin, S., Sriboonlue, P. and Kukongviriyapan, V. 2008. Uricosuric effect of roselle (Hibiscus sabdariffa) in normal and renal-stone former subject. Journal of Ethnopharmacology. 117: 491–495. (Journal)
Prisco, J.T., Babtista, C.R. and Pinheiro, J. L. 1992. Hydration dehydration seed Pre- treatment and its effects on seed germination under water stress condition. Plant, Cell and Environment. 15: 31-35. (Journal)
Rajasekaran, L.R.A., Stiles, M.A., Surette, A.V., Sturz, T.J., Blake, C. and Nowak, J. 2002. Effect of various temperature regimes on germination and the role of salicylates in promoting germination at low temperatures. Canadian Journal of Plant Science. 82: 443-450. (Journal)
Seghatoleslami, M. J., Mosavi, S.G. and Barzegaran, T. 2013. Effect of irrigation levels and planting date on yield and water use efficiency of Hibiscus sabdariffa L. Iranian Journal of Medicinal and Aromatic Plants. 29: 144-156. (In Persian) )Journal)
Senaranta, T., Teuchell, D., Bumm, E. and Dixon, K. 2002. Acetyl salicylic acid (aspirin) and salicylic acid induce multiple stress tolerance in bean and tomato plants. Plant Growth Regulation. 30: 157-161. (Journal)
Shakirova, F.M., Sahabutdinova, A.R., Bezrukova, M.V., Fatkhutdionova, R.A. and Fathkhutdiniva. D. R. 2003. Change in hormonal status of wheat seedling induced by salicylic acid and salinity. Plant Science. 164: 317-322. (Journal)
Shi, Q. and Zhu, Z. 2008. Effects of exogenous salicylic acid on manganese toxicity, element contents and antioxidative system in cucumber. Environmental and Experimental Botany. 63: 317-326. (Journal)
Singh, B. and Usha. K. 2003. Salicylic acid induced physiological and biochemical changes in wheat seedlings under water stress. Plant Growth Regulation. 39: 137-141. (Journal)