تأثیر پرایمینگ بر مؤلفه های جوانه زنی و رشد گیاهچه ماش (Vigna radiata) (اکوتیپ شوشتر) تحت شرایط تنش شوری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد زراعت، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه رامین اهواز

2 گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه رامین اهواز

چکیده

به­منظور بررسی اثر پرایمینگ بر مؤلفه­های جوانه­زنی و رشد گیاهچه ماش توده محلی استان خوزستان تحت شرایط تنش شوری آزمایشی در سال 1393 در آزمایشگاه تکنولوژی بذر دانشگاه کشاورزی و منابع طبیعی رامین خوزستان به اجرا در آمد آزمایش به­صورت فاکتوریل در قالب طرح کاملاً تصادفی در سه تکرار اجرا شد. تیمارهای آزمایشی دو عامل شوری در چهار سطح صفر (شاهد)، 6، 12 و 18 دسی­زیمنس بر متر با استفاده از نمک کلریدسدیم  و نوع پرایمینگ بذر در هفت سطح شامل ­اسید جیبرلیک (25 میلی­گرم بر کیلوگرم)، کربنات­کلسیم (20 گرم در لیتر)، ­اسید سالیسیلیک (25 میلی­گرم بر کیلوگرم)، پلی­اتیلن گلیکول (15- بار)، آب معمولی (04/8=pH و 41/2 دسی‌زیمنس بر متر=Ec)، آب مقطر و شاهد (بدون پرایمینگ) بود. صفات مورد بررسی شامل درصد و سرعت جوانه‌زنی، طول ریشه‌چه و ساقه‌چه، طول گیاهچه، نسبت طول ریشه‌چه به ساقه‌چه، شاخص بنیه گیاهچه و وزن خشک گیاهچه مورد بررسی قرار گرفت. نتایج مقایسه میانگین حاکی از آن بود که سطوح شوری سبب کاهش مؤلفه­های جوانه­زنی بذر و رشد گیاهچه در تمام بذور پرایم شده و پرایم نشده شد. همچنین پرایمینگ سبب بهبود مؤلفه­های جوانه­زنی در تمام سطوح شوری نسبت به شاهد (بدون پرایم) شدند. در این بین پرایمینگ با کربنات کلسیم کارایی بیش­تری در کاهش اثر تنش شوری بر مؤلفه­های جوانه­های زنی بذر و رشد گیاهچه داشت به نحوی­که بذور پیش تیمار شده با این روش نسبت به سایر روش‌های استفاده شده از افت کم­تری در مؤلفه­های مذکور برخوردار بود.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of priming on the germination and growth of the Vigna radiata (Shushtar ecotype) seeding under salinity stress

نویسندگان [English]

  • Mohammad Abbasi Bideli 1
  • Alireza Ebdali Mashhadi 2
چکیده [English]

In order to study the effect of priming on the germination and growth parameters of vetch seedling under the salinity stress, an experiment was carried out in seed Technology Laboratory of Ramin's Natural Resources and Agriculture University, Khuzestan. The experiment was carried with three replications. The salinity treatments were applied in four levels: o (Control), 6,12 and 18 DS/m by levels of priming of different resources including gibberellic acid (25 mg/kg), calcium carbonate (20g/L), salicylic acid (25 mg/kg), polyethylene glycol (15 times), water(PH=8.04, EC=2.41), distillated water and control (without priming). The results showed that the different levels of salinity lead to decrease the seed germination and seedling growth parameters in all primed and non-primed seeds. Moreover, the priming caused to improve the germination in all levels of salinity compared to control group (without priming). Among these, the prime of calcium carbonate had a positive role in reducing the effect of salinity stress on the parameters such as seed germination and seedling growth so that the primed seeds by calcium carbonate had less reduction in above-mentioned parameters than other primed seeds

کلیدواژه‌ها [English]

  • Calcium carbonate
  • gibberellic acid
  • Polyethylene glycol
  • Salicylic acid
Abdul Kader, M. and Lindberg, S. 2010. Cytosolic calcium and pH signaling in plants under salinity stress. Plant Signal Behav. 5(3): 233–238. (Journal)
Ahmadvand, G., Soleymani F., Saadatian B.and Pouya M. 2012. Effects of Seed Priming on Seed Germination and Seedling Emergence of Cotton Under Salinity Stress. World Applied Sciences Journal 20 (11): 1453-1458. (In Persian)(Journal)
Almansouri, M., Kinet, J. M. and Lutts, S. 2001. Effect of salt and osmotic stresses on germination in durum wheat (Triticum durum Desf.). Plant and Soil, 231: 243-254. (Journal)
Aloui, H., Souguir M. and Hannachi, C. 2014. Determination of an optimal priming duration and concentration protocol for pepper seeds (Capsicum annuum L.). Acta agriculturae Slovenica, 103(2): 213 – 221. (Journal)
Awada, S., Campbel, W. F., Dudley, L. M. and Jurinak, J. J. 1995. Interactive effects of sodium chloride, sodium sulfate, calcium sulfate and calcium chloride on snap bean growth, photosynthesis and ion uptake. Journal of Plant Nutrition, 18: 889-900. (Journal)
Aydinalp, C. and Marinova, S. 2009. The effects of heavy metals on seed germination and plant growth on alfalfa plant (Medicago Sativa). Bulgarian Journal of Agricultural Science, 15(4): 347-350. (Journal)
Bahmani, R., Bihamta M. R., Habibi D. and Forozesh P. 2013. Evaluation of germination, root and shoot growth under cadmium stress for different bean genotypes (Phaseolus vulgaris L.). Iranian Journal of Agronomy and Plant Breeding, 8(4): 145-154. (In Persian)(Journal)
Belur B. V., Merwade M. N., Channaveerswami A. S., Krishna A., Rudranaik V. and Tirakannavar S. 2010. Effect of pre-sowing seed treatments with calcium salts and their concentrations on crop growth, seed yield and quality of soybean (Glycine max L.). Karnataka Journal of Agricultural Sciences, 23(4): 642-646. (Journal)
Ben Dkhil, B., Issa, A. and Denden, M. 2014. Germination and Seedling Emergence of Primed Okra (Abelmoschus esculentus L.) Seeds under Salt Stress and Low Temperature. American Journal of Plant Physiology, 9: 38-45. (Journal)
Bewley, J. D. and Blak, M. 1998. Seed: physiology of development and germination. Second edition. Plenum press, New York. (Book)
Bhatt, A., Rawal, R. S. and Dhar, U. 2005. Germination improvement in Swertia angustifolia: a high value medicinal plant of Himalaya. Current Science, 89: 1008-1012. (Journal)
Demir Kaya, M., Okçu, G., Atak, M. and Çıkılı, Y., and Kolsarıcı, Ö. 2006. Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). European Journal of Agronomy, 24 (4): 291-295. (Journal)
Elouaer, M. A. and Hannachi, C. 2012. Seed priming to improve germination and seedling growth of safflower (Carthamus tinctorius) under salt stress. EurAsian Journal of BioSciences, 6: 76-84. (Journal)
Ghaneie Motlagh, Gh., Pashaee Aval, A., Khormali, F. and Mosaedi, A. 2010. Investigating effect of some amendments on soil chemical properties in a saline-sodic soil. Watershed Management Research Journal (Pajouhesh & Sazandegi), 86: 24-31. (In Persian) (Journal)
Hadi, M. R. and Karimi N. 2012. The role of calcium in plant's salt tolerance. Journal of Plant Nutrition, 35(13): 2037-2054. (In Persian)(Journal)
Hashemi Dezfuli, A., Koocheki A., Banaian M. 1995. Increase crop yield. Mashhad University of jihad publications. 287 pages. (In Persian) (Journal)
Islam, F., Yasmeen T., Ali S., Ali B., Farooq, M. A. and Gill, R. A. 2015. Priming-induced antioxidative responses in two wheat cultivars under saline stress. Acta Physiologiae Plantarum, 37:153. (Journal)
Khalessroo, Sh. and Aghaalykhany, M. 2007. Effect of salinity and water stress on seed germination of sorghum and pearl millet. Pajouhesh va Sazandgi, 77: 153 -163. (In Persian) (Journal)
Kolodziejek, J. and Patykowski, J. 2015. The effect of temperature, light and calcium carbonate on seed germination and radicle growth of the polycarpic perennial Galium cracoviense (Rubiaceae), a Narrow endemic species from southern Poland. Acta Biologica Cracoviensia Series Botanica, 57(1): 70-81. (Journal)
Kim, S. H. and Kang, C. 1987. Vigor determination in barley seed by the multiple criteria. Korean Journal of Crop Science, 32: 417-427. (Journal)
Mc Donald, M. B. 2000. Seed priming. In: Black, M., Bewley, J. D. (Eds.). Seed Technology and its Biological Basis. Sheffield Academic Press. Sheffield, UK. pp: 287-325. (Journal)
Murata, M. R., Zharare G. E. and Hammes, P. S. 2008. Pelleting or Priming Seed with Calcium Improves Groundnut Seedling Survival in Acid Soils. Journal of Plant Nutrition, 31(10): 1736-1745. (Journal)
Nawaz, J., Hussain, M., Jabbar, A., Nadeem, G. A., Sajid, M., Mashoodul, S. and Shabbir, I. 2013. Seed Priming A Technique. International Journal of Agriculture and Crop Sciences. 6(20): 1373-1381. (Journal)
Nichols, M. A. and Heydecker, W. 1986. Two approaches to the study of germination date. Proceedings of the International Seed Testing Association, 33(3): 531-540. (Journal)
Parida, A. K. and Das, A. B. 2005. Salt tolerance and salinity effects on plants: a review. Ecotoxicology and Environmental Safety, 60(3): 324-349. (Journal)
Patade, V. Y., Maya, K. and Zakwan, A. 2011. Seed priming mediated germination improvement and tolerance to subsequent exposure to cold and salt stress in capsicum. Research Journal of Seed Science, 4 (3): 125-136. (Journal)
Rahimi, A. 2012. Effect of osmopriming and irrigation regime on yield quantity and essential oil content of cumin (Cuminum ciminum L.). Iranian Journal of Medicinal and Aromatic Plants, 28 (1): 141-131. (In Persian) (Journal)
Rahimian Mashhadi, H. Bagheri Kazemabad A. and Paryab A. 1991. Effects PEG and NaCl Induced water Potential at different temperatures on germination and seedling vigor of several of wheat (Triticum spp.) populations. Agricultural Science and Technology, 5(1): 37-46. (In Persian) (Journal)
Ritchie, S. and Gilroy, S. 1998. Gibberellins: regulating genes and germination. New Phytologist, 140(3): 363-383. (Journal)
Saed-Moocheshia, A., Shekoofaa, A., Sadeghib, H. and Pessaraklic, M. 2014. Drought and Salt Stress Mitigation by Seed Priming with KNO3 and Urea in Various Maize Hybrids: An Experimental Approach Based on Enhancing Antioxidant Responses. Journal of Plant Nutrition, 37(5): 674-689. (Journal)
Soltani, E. Akram-Ghaderi, F. and Maemar, H. 2007. The effect of priming on germination components and seedling growth of cotton seeds under drought. Journal of Agricultural Science and Natural Resources, 14(5): 9-16. (Journal)
Tabatabaei, S. A. 2014. The effects of salinity stress on seed reserve utilization and germination percentage of treated seed of barley (Hordeum vulgare L.). Cercetari Agronomice in Moldova, 47(1): 23-29. (Journal)
Talebzadeh, Z. and Lahooti. 2004. Seed germination Lycopersicum esculeutum under salinity stress. Abstracts of Congress on Applied Biology. Mashhad University. (In Persian) (Journal)
Yarnia, M. 2007. Evaluation of possibly of forage sorghum production in saline condition with CaCO3 application. Agroecology Journal, 2(4): 77-91. (In Persian) (Journal)
Yarnia, M. Heydari, H. and Rahimzadeh Khoiy, F. 2006. Effects of CaCo on alfalfa salinity tolerance. Agroecology Journal, 1(2): 9-21. (In Persian) (Journal)
Zare, M., Mehrabi Oladi, A. and Sharaf Zadeh, S. 2006. Effects of gibberellic acid and kinetin on wheat germination and seedling growth under salinity stress. Journal of Agricultural Sciences, 12 (4): 856-864. (In Persian) (Journal)
Zehra, A., Gul, B., Ansari, R., and Khan, M. A. 2012. Role of calcium in alleviating effect of salinity on germination of Phragmites karka seeds. South African Journal of Botany, 78: 122-128. (Journal)