ارزیابی ویژگیهای کمی و بیوشیمیایی جوانه زنی بذر ژنوتیپ‌های کینوا (Chenopodium quinoa Willd.) تولید شده در شرایط آب و هوایی کرج

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد علوم و تکنولوژی بذر، گروه زراعت و اصلاح نباتات، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران

2 دانشیار، گروه زراعت و اصلاح نباتات، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران

3 استاد، گروه زراعت و اصلاح نباتات، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران

4 استادیار پژوهش، موسسه تحقیقات اصلاح و تهیه نهال و بذر، سازمان تحقیقات آموزش و ترویج کشاورزی، کرج، ایران

10.22124/jms.2023.7682

چکیده

کینوا Chenopodium quinoa Willd. به­دلیل ارزش بالای غذایی، تنوع ژنتیکی و سازگاری با شرایط محیطی متفاوت و تنش‌زا، نقش مهمی در تامین امنیت غذایی در شرایط آب و هوایی در حال تغییر ایفا می­نماید. هدف از انجام این پژوهش، ارزیابی ویژگی‌های جوانه­زنی و بیوشیمیایی 12 ژنوتیپ کینوا سازگار با شرایط آب و هوایی ایران بود. این آزمایش در قالب طرح بلوک­های کامل تصادفی با سه تکرار، در سال 1401 در آزمایشگاه فیزیولوژی و زیست فناوری بذر دانشکده علوم کشاورزی دانشگاه گیلان انجام شد. در این آزمایش صفات درصد جوانه­زنی، سرعت جوانه­زنی، یکنواختی جوانه­زنی، D10، D50، D90، وزن­تر و خشک ریشه­چه و ساقه­چه و گیاهچه، طول ریشه­چه و ساقه­چه و گیاهچه، هدایت الکتریکی محلول بذر، محتوای مالون دی‌آلدئید، فعالیت آنزیم آلفا آمیلاز و محتوای ساپونین مورد بررسی قرار گرفتند. نتایج تجزیه واریانس داده­ها نشان­دهنده وجود تفاوت معنی­دار بین ژنوتیپ­ها در تمام صفات و شاخص‌های مورد مطالعه بود. ژنوتیپ Q26 در صفات درصد جوانه­زنی (33/91 درصد)، سرعت جوانه­زنی، طول ریشه­چه و ساقه­چه و گیاهچه، وزن تر گیاهچه، فعالیت آنزیم آلفا آمیلاز (234/0 میکرومول گلوکز آزادشده در گرم وزن بذر) و محتوای ساپونین نسبت به سایر ژنوتیپ‌ها برتری داشت. از نظر شاخص‌های یکنواختی جوانه­زنی، هدایت الکتریکی محلول بذر و محتوای مالون­دی‌آلدئید، ژنوتیپ Q26 پائین­ترین رتبه و به­ترتیب ژنوتیپ­های Q2 و Q12 بالا­ترین رتبه را داشتند. در بین ژنوتیپ­های مورد مطالعه، ژنوتیپ Q26 نتایج بهتری در مقایسه با ژنوتیپ­های دیگر نشان داد. به­ نظر می­رسد این ژنوتیپ می­تواند شرایط بهتری جهت کاشت در شرایط آب و هوایی ایران داشته باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of quantitative and biochemical characteristics of seed germination of quinoa genotypes (Chenopodium quinoa Willd.) produced in the climatic conditions of Karaj

نویسندگان [English]

  • Sadaf GilaniNia 1
  • Seyed MohammadReza Ehteshami 2
  • Masoud Esfahani 3
  • Mahmood Bagheri 4
1 M.Sc. Student of Seed Science and Technology, Department of Agronomy and Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
2 Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
3 Professor, Department of Agronomy and Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
4 Assistant Professor, Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
چکیده [English]

Quinoa, Chenopodium quinoa Willd, plays a strategic role in establishing food security under changing climatic conditions due to its nutritional value, genetic diversity, and high adaptability under stressful conditions and different environments. The purpose of this research was to evaluate the germination and biochemical characteristics of 12 quinoa genotypes adapted to the climatic conditions of Iran. This experiment was conducted in the form of the randomized complete blocks design with three replications in the Laboratory of Seed Physiology and Biotechnology, Faculty of Agricultural Sciences, Guilan University. In this experiment, the characteristics of germination percentage, germination rate, germination uniformity, D10, D50, D90, radicle fresh and dry, shoot and seedling weight, radicle, shoot, and seedling length, electrical conductivity of seed solution, malondialdehyde content, alpha-amylase enzyme activity and saponin content were investigated. The analysis of the data's variance demonstrated significant differences between genotypes in all studied traits. Q26 Genotype showed high values in germination percentage (91.33%), germination rate, radicle, shoot and seedling length, seedling fresh weight, alpha-amylase enzyme activity (0.234 micromoles released glucose per one gram of seed weight) and saponin content. Q26 genotype had the worst, and Q2 and Q12 genotypes had the best performance in terms of germination uniformity, electrical conductivity of seed solution and malondialdehyde content, respectively. Among the studied genotypes, the Q26 genotype demonstrated better results in comparison with other genotypes. It seems that this genotype may have better conditions for planting in the climatic conditions of Iran

کلیدواژه‌ها [English]

  • Alpha amylase
  • Electri cal conductivity
  • Germination
  • Malondialdehyde
  • Quinoa
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