اثر انواع روش های خراش‌دهی پوسته، لیزر هلیم- نئون و سدیم نیتروپروساید بر شکست خواب بذر و رشد گیاهچه گیاه مورد (Myrtus commnius L.)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استاد، گروه زیست گیاهی و مرکز تحقیقات بیوتکنولوژی دانشگاه شهرکرد، شهرکرد، ایران

2 گروه زیست گیاهی، دانشکده علوم، دانشگاه شهرکرد، شهرکرد، ایران

10.22124/jms.2023.7610

چکیده

مورد یک گیاه دارویی است که دانه­های آن دارای خواب اولیه هستند. در تحقیق حاضر ابتدا اثر شیوه­های مختلف خراش­دهی (تیمارهای اسید سولفوریک ۷۰ و 90 درصد، سنباده­زنی به­مدت ۵ و10 دقیقه، خراش با تیغ و خیساندن در آب داغ به­مدت ۱۰ و 20 دقیقه) روی شکست خواب بذر ارزیابی شد. نتایج نشان داد که بدون خراش­دهی، جوانه­زنی دانه­ها بسیار کم بود، امّا همه شیوه­های خراش­دهی، در حد معنی­داری جوانه­زنی نهایی را افزایش دادند. در بین سطوح مختلف تیمارها، حداکثر جوانه­زنی نهایی با 20 دقیقه خیساندن در آب داغ (33درصد)، 5 دقیقه تیمار با اسید سولفوریک 90 درصد (59 درصد)، 10 دقیقه سنباده­زدن (63 درصد) و خراش­دهی با تیغ (65 درصد) به­دست آمد. آزمایش بعدی نشان داد که دانه­های مورد، فتوبلاستیک مثبت هستند و تیمار با لیزر هلیم - نئون بیش­تر از نور سفید و قرمز، جوانه­زنی همه دانه­های خراش­دهی شده با شیوه­های فوق را افزایش داد و جوانه­زنی بذرهای سنباده­زده و خراش­دهی­شده با تیغ را به­حدود 85 درصد رساند. نتایج آخرین آزمایش، هم نشان داد غلظت­های 5/0درصد نیترات پتاسیم و100 میکرومولار سدیم نیتروپروساید (SNP به­عنوان یک ترکیب آزاد کننده (NO) جوانه­زنی، طول و بنیه دانه­رست­های رشد یافته از همه دانه­های خراش­دهی شده را افزایش داد و جوانه­زنی بذرهای سنباده­زده و خراش­دهی شده با تیغ را به حدود 80 درصد رساند. این نتایج نشان دادند که دانه­های مورد یک خواب ترکیبی شامل خواب فیزیکی و فیزیولوژیک دارند که می­تواند با سنباده­زدن و سپس تیمار با سدیم نیتروپروساید یا تابش لیزر هلیم- نئون رفع شود.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of various scarification methods, He-Ne laser and sodium nitroprusside on seed dormancy breaking and the growth of myrtle (Myrtus commnius L.) seedlings

نویسندگان [English]

  • Rayhaneh Amooaghaie 1
  • Azadeh Rafieii 2
1 Professor, Plant Science Department and Biotechnology Research Center, University of Shahrekord, Shahrekord, Iran
2 Plant Science Department, Faculty of Science, University of Shahrekord, Shahrekord, Iran
چکیده [English]

Myrtus communis is a medicinal plant that its seeds have primary dormancy. Therefore, in current study, firstly effect of various types of scarification (treatment with H2SO4 70 or 90% or sand paper for 5 and 10 min, blade and soaking in hot water for 10, 20 min) were evaluated on seed dormancy breaking. Results showed that without scarification, seed germination was very low, whereas various types of scarification significantly increased final seed germination. Among various levels of treatments, the highest seed germinatin obtained by soaking in hot water for 20 min (33%), treatment with  H2SO90% for 5 min (59%), sand paper for 10 min (63%) and blade (65%). The next experiment showed that myrtle seeds were photoblastic and He-Ne laser more than white or red light increased germination of scarified seeds by above methods. He-Ne laser irradiation reached seed germination of scarified seeds by sand paper and blade to 80%. Results of the last experiment also showed that application of concentrations of 0.5% KNO3 and 100 µM sodium nitroprusside (SNP as a NO donor) also increased seed germination, seedling length and vigor of seedlings grown from all scarified seeds and seed germination of scarified seeds by sand paper and blade achieved to 80%. These results showed that myrtle seeds have combination of physical and physiological dormancy that can be attenuated with scarification by sand paper and treatment with SNP or He-Ne laser irradiation.

کلیدواژه‌ها [English]

  • Blade
  • Light
  • Hot water
  • Nitrate
  • Sand paper
  • Seed vigor
  • Sulfuric acid
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