بهبود خصوصیات فیزیولوژیک و بیوشیمیایی آرابیدوبسیس تالیانا با انتقال تراژن آرتمین

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه بیوتکنولوژی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت

10.22124/jms.2023.6172

چکیده

دمای بالا سبب آسیب­های جدی در گیاهان از جمله اختلال در تعادل درون سلولی، کندی یا رکود رشد و نمو و حتی مرگ می‌شود. هنگامی که گیاهان در معرض دمای بالا قرار می‌گیرند، پروتئین­های پاسخ گرمایی  (HSP)در سلول فعال می­شوند. از همین گروه، HSPهای کوچک (sHSP) قرار دارند که با افزایش دما فعال شده، و در نقش چاپرون مولکولی به پروتئین‌هایی که در معرض تنش گرمایی بوده‌اند متصل می‌شوند و مانع از تاشدن نادرست یا به عبارتی به تاخوردگی صحیح آن­ها کمک می‌کنند. پروتئینی به نام آرتمین در سخت پوست  Artemia urmianaشناسایی شد که از مهمترین خصوصیات آن قدرت چپرونی بـالا و وجود دمین α-crystallin است که وجه مشخصهsHSPها است. به‌منظور مطالعات ژنومیکس کارکردی شامل فنوتیپ و شاخص‌های تحمل گیاهان، تراژن آرتمین به گیاه مدل آرابیدوپسیس تالیانا منتقل و واکنش گیاهان موتانت در قالب یک طرح آزمایشی به‌صورت فاکتوریل و طرح پایه کاملاً تصادفی در سه تکرار درآزمایشگاه بیوتکنولوژی مطالعه شد. تنش گرمایی در دمای 45 درجه سلسیوس اعمال و مطالعه مولفه­های بیوشیمیایی، مولفه­های جوانه­زنی و فیزیولوژیک نشان داد که برهمکنش فاکتورهای تنش گرمایی و ژنوتیپ بر تمام صفات مربوط به جوانه­زنی، وزن­تر و وزن خشک گیاهچه، طول گیاه­چه و محتوای پروتئین کل، آنزیم کاتالاز و محتوای مالون­دی­آلدئید در سطح یک درصد معنی‌دار بودند. با اعمال تنش گرمایی گیاهان حاوی تراژن آرتمین به طور معنی­داری نسبت به گیاهان غیرتراریخته جوانه­زنی و رشد گیاه­چه بهتری را نشان دادند. تنش همچنین موجب افزایش معنی‌دار فعالیت آنزیم کاتالاز و محتوای پروتئین کل و کاهش محتوای مالون­دی‌آلدئید در گیاهان تراریخته در مقایسه با گیاه غیرتراریخته شد. بر این اساس، حضور تراژن آرتمین در گیاهان احتمالا سبب افزایش مقاومت گیاهچه‌ به تنش گرمایی و افزایش عملکرد می‌شود.

کلیدواژه‌ها


عنوان مقاله [English]

Improvement of physiological and biochemical properties of Arabidopsis thaliana by transfer of artemin transgene

نویسندگان [English]

  • Tayyebeh Fallahi Pashaki
  • Mohammad Mahdi Sohani
  • Reza Shirzadian-Khoramabad,
Department of Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
چکیده [English]

High temperatures causes serious damage to plants, including disruption of cell hemostasis, slowness or stagnation of growth and development, and even death. When plants are exposed to high temperatures, heat shock proteins (HSP) are induced in the cell. Among HSPs are Small heat shock proteins (sHSP), which act as chaperones. They bind to proteins that have been exposed to heat stress and prevent them from incorrectly folding. The Artemin protein isolated from Artemia urmiana has a a-crystallin domain, which is characteristic of sHSP. In order to characterize the functional genomic roles of the protein in plants, artemin gene was transferred to Arabidopsis. A statistical analysis using the factorial design method was performed including two independent transgenic genotypes (art-1 and art-2) along with a wild genotype Col-0 under heat stress at 45°C. Results showed that the interaction between heat stress factors and genotypes was significant at the 1% level for most traits including germination, seedling wet and dry weight, seedling length, total protein content, catalase enzyme activity, and malondialdehyde content. All the components of germination were adversely affected by the application of heat stress. However, the transgenics still had a significantly higher seed germination and plant growth than the wild-type plants. As a result of heat stress, transgenic plants had significantly higher catalase enzyme activity and total protein content, and lower malondialdehyde levels compared with wild type plants. The results indicated that Artemin transgen in plants may cause significantly higher plant resistance to heat stress and increase plant yield.

کلیدواژه‌ها [English]

  • Abiotic stress
  • Artemin
  • seed germination
  • Total protein content
  • sHSP
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