اثر پرایمینگ با اسید آسکوربیک بر بهبود ویژگی‌های جوانه‌زنی و تحرک ذخایر غذایی بذرهای پیر شده ذرت

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکترای اکولوژی گیاهان زراعی، دانشگاه محقق اردبیلی

2 استاد فیزیولوژی گیاهان زراعی، دانشگاه محقق اردبیلی

3 دانش آموخته کارشناسی‌ارشد علوم و تکنولوژی بذر دانشگاه محقق اردبیلی

4 دانش آموخته کارشناسی ارشد زراعت و اصلاح نباتات دانشگاه محقق اردبیلی

10.22124/jms.2022.6151

چکیده

زوال و فرسودگی بذر در طی انبارداری مهمترین عامل خسارت به بذر می­باشد. به منظور بررسی اثر اسید آسکوربیک (ویتامین C) بر بهبود ویژگی­های جوانه­زنی و تحرک ذخایر غذایی بذرهای پیر شده ذرت، آزمایشی در سال ۱۳۹۶ در دانشگاه محقق اردبیلی به صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار اجرا شد. تیمارهای آزمایش شامل مدت زمان پیری (صفر، ۶ و ۱۲ روز پیر شده) و پرایمینگ با چهار غلظت اسید آسکوربیک (صفر، ۵۰، ۱۰۰و ۱۵۰ میلی­گرم در لیتر) بود. صفات اندازه­گیری شده در این آزمایش، درصد و سرعت جوانه­زنی، وزن خشک ریشه­چه، ساقه­چه و وزن خشک باقی­مانده، بنیه گیاهچه، مقدار استفاده از ذخایر (SRUR[1]1)، کسر ذخایر مصرف شده (FUSR2)، کارایی استفاده از ذخایر (SRUE3)، شاخص تنفس و فعالیت آنزیم کاتالاز بود. نتایج آزمایش نشان داد که پیری بذرها در هر دو سطح (۶ و ۱۲ روز) باعث کاهش صفات اندازه­گیری شده گردید. استفاده از پرایمینگ صفات اندازه­گیری شده را بهبود بخشید. غلظت ۱۵۰ میلی­گرم در لیتر اسید آسکوربیک با درصد جوانه­زنی ۲۲/۷۸ درصد، بنیه گیاهچه ۶/۱۱، SRUR ۶۵/۵ میلی­گرم بر بذر، FUSR ۸۴/۰، SRUE ۰۲۵/۰ میلی­گرم بر میلی­گرم و شاخص تنفس ۵۱/۵ گرم بهترین غلظت استفاده شده بود. برای آنزیم کاتالاز اثر اسید آسکوربیک معنی­دار نبود، بیشترین میزان فعالیت آنزیم کاتالاز در تیمار ۱۲ روز پیری بدست آمد. در کل کاربرد ABA موجب افزایش بنیه بذرهای پیر شده گردید.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of priming with ascorbic acid on improvement of germination characteristics and mobility of reservoirs in corn aging seeds

نویسندگان [English]

  • Soghra Ghahremani 1
  • Mohammad Sedghi 2
  • Seyyedeh Roghayyeh KHatami 3
  • Fatemeh Ahmadnia 4
  • Mohammad Goodarzi, 4
1 Department of Agronomy and Plant Breeding, Faculty of Agriculture and natural resources, University of Mohaghegh Ardabili, Ardabil, Iran
2 Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Iran
3 Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Iran
4 Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Iran
چکیده [English]

The deterioration of seed during storage is the most important cause of seed damage. In order to investigate the effect of ascorbic acid (vitamin C) on improvement of germination characteristics and mobility of reservoirs in corn aging seeds, a factorial experiment was conducted in a completely randomized design with three replications in 2017 at the University of Mohaghegh Ardabili. Treatments included three levels of aging (0, 6 and 12 days) and priming with four concentrations of ascorbic acid (0, 50, 100 and 150 mg lit-1). The traits measured in this experiment were percentage and rate of germination, dry weight of radicle, dry weight of plumule and seed residual, seedling vigor, seed reserve utilization rate (SRUR), fraction of used seed reserves (FUSR), Seed reserve utilization efficiency (SRUE), respiration index and catalase activity. The results of the experiment showed that the aging of the seeds at both levels (6 and 12 days) decreased the measured traits. The use of priming treatment improved the measured traits. Concentration of 150 mg lit-1 ascorbic acid considering germination percentage of 78.22%, seedling vigor 11.6, SRUR 65.5 mg seed-1, FUSR 0.84, SRUE 0.25 mg mg-1 and respiration index 5.51 g was the best. The effect of ascorbic acid on the catalase enzyme was not significant. The highest amount of catalase enzyme activity was obtained in 12 days of aging. In general, the use of ABA increased vigor of aging seeds.

کلیدواژه‌ها [English]

  • Catalase
  • Controlled aging
  • Respiration index
  • Seed reserve utilization efficiency
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