اثر پرایمینگ با اسید آسکوربیک بر بهبود ویژگی‌های جوانه‌زنی و تحرک ذخایر غذایی بذرهای پیر شده ذرت

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکترای اکولوژی گیاهان زراعی، دانشگاه محقق اردبیلی

2 استاد فیزیولوژی گیاهان زراعی، دانشگاه محقق اردبیلی

3 دانش آموخته کارشناسی‌ارشد علوم و تکنولوژی بذر دانشگاه محقق اردبیلی

4 دانش آموخته کارشناسی ارشد زراعت و اصلاح نباتات دانشگاه محقق اردبیلی

10.22124/jms.2022.6151

چکیده

زوال و فرسودگی بذر در طی انبارداری مهمترین عامل خسارت به بذر می­باشد. به منظور بررسی اثر اسید آسکوربیک (ویتامین C) بر بهبود ویژگی­های جوانه­زنی و تحرک ذخایر غذایی بذرهای پیر شده ذرت، آزمایشی در سال ۱۳۹۶ در دانشگاه محقق اردبیلی به صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار اجرا شد. تیمارهای آزمایش شامل مدت زمان پیری (صفر، ۶ و ۱۲ روز پیر شده) و پرایمینگ با چهار غلظت اسید آسکوربیک (صفر، ۵۰، ۱۰۰و ۱۵۰ میلی­گرم در لیتر) بود. صفات اندازه­گیری شده در این آزمایش، درصد و سرعت جوانه­زنی، وزن خشک ریشه­چه، ساقه­چه و وزن خشک باقی­مانده، بنیه گیاهچه، مقدار استفاده از ذخایر (SRUR[1]1)، کسر ذخایر مصرف شده (FUSR2)، کارایی استفاده از ذخایر (SRUE3)، شاخص تنفس و فعالیت آنزیم کاتالاز بود. نتایج آزمایش نشان داد که پیری بذرها در هر دو سطح (۶ و ۱۲ روز) باعث کاهش صفات اندازه­گیری شده گردید. استفاده از پرایمینگ صفات اندازه­گیری شده را بهبود بخشید. غلظت ۱۵۰ میلی­گرم در لیتر اسید آسکوربیک با درصد جوانه­زنی ۲۲/۷۸ درصد، بنیه گیاهچه ۶/۱۱، SRUR ۶۵/۵ میلی­گرم بر بذر، FUSR ۸۴/۰، SRUE ۰۲۵/۰ میلی­گرم بر میلی­گرم و شاخص تنفس ۵۱/۵ گرم بهترین غلظت استفاده شده بود. برای آنزیم کاتالاز اثر اسید آسکوربیک معنی­دار نبود، بیشترین میزان فعالیت آنزیم کاتالاز در تیمار ۱۲ روز پیری بدست آمد. در کل کاربرد ABA موجب افزایش بنیه بذرهای پیر شده گردید.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of priming with ascorbic acid on improvement of germination characteristics and mobility of reservoirs in corn aging seeds

نویسندگان [English]

  • Soghra Ghahremani 1
  • Mohammad Sedghi 2
  • Seyyedeh Roghayyeh KHatami 3
  • Fatemeh Ahmadnia 4
  • Mohammad Goodarzi, 4
1 Department of Agronomy and Plant Breeding, Faculty of Agriculture and natural resources, University of Mohaghegh Ardabili, Ardabil, Iran
2 Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Iran
3 Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Iran
4 Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Iran
چکیده [English]

The deterioration of seed during storage is the most important cause of seed damage. In order to investigate the effect of ascorbic acid (vitamin C) on improvement of germination characteristics and mobility of reservoirs in corn aging seeds, a factorial experiment was conducted in a completely randomized design with three replications in 2017 at the University of Mohaghegh Ardabili. Treatments included three levels of aging (0, 6 and 12 days) and priming with four concentrations of ascorbic acid (0, 50, 100 and 150 mg lit-1). The traits measured in this experiment were percentage and rate of germination, dry weight of radicle, dry weight of plumule and seed residual, seedling vigor, seed reserve utilization rate (SRUR), fraction of used seed reserves (FUSR), Seed reserve utilization efficiency (SRUE), respiration index and catalase activity. The results of the experiment showed that the aging of the seeds at both levels (6 and 12 days) decreased the measured traits. The use of priming treatment improved the measured traits. Concentration of 150 mg lit-1 ascorbic acid considering germination percentage of 78.22%, seedling vigor 11.6, SRUR 65.5 mg seed-1, FUSR 0.84, SRUE 0.25 mg mg-1 and respiration index 5.51 g was the best. The effect of ascorbic acid on the catalase enzyme was not significant. The highest amount of catalase enzyme activity was obtained in 12 days of aging. In general, the use of ABA increased vigor of aging seeds.

کلیدواژه‌ها [English]

  • Catalase
  • Controlled aging
  • Respiration index
  • Seed reserve utilization efficiency
1. Abdul-Baki, A.A. and Anderson, J.D. 1973. Vigor determination in soybean by multiple criteria. Crop Science. 13: 630-633. (Journal) 2. Aebi, H. 1984. Catalase in vitro. Method of Enzymology. 105:121-126. (Journal) 3. Alivand, R., Tavakkol Afshari, R. and Sharifzadeh, F. 2013. Effects of gibberellin, salicylic acid and ascorbic acid on improvement of germination characteristics of deteriorated seeds of Brassica Napus. Iranian Journal of Field Crop Science. 43: 561-571. (In Persian) (Journal) 4. Alizadeh, A. 1997. Water, soil and plant relationships. Astan Quds Razavi Press. 427 page. (Book) 5. Basra, S.M.A., Pannu, I.A. and Afzal, I. 2003. Evaluation of seedling vigour of hydro and matriprime wheat (Triticum aestivum L.) seeds. International Journal of Agriculture and Biology. 5:121- 125. (Journal) 6. Black, M. and Bewley, J. D. 2009. Seed Technology and its Biological Basis. Translated by Tavakkol Afshari, R., Abbasi Surki, A. and Ghasemi, E. University of Tehran Press. 515 page. (Book) 7. Bradford, K.J. 1986. Manipulation of seed water relation via osmotic priming to improve germination under stress conditions. Horticultural Science. 21:1105-1111. (Journal) 8. Burguieres, E., McCue, P., Kwon, Y. and Shetty, K. 2007. Effect of vitamin c and folic acid on seed vigourrespons and phenolic activity. Bioresource Technology. 98:1393-1400. (Journal) 9. De Figueiredo, E., Albuquerque, M.C. and De Carvalho, N.M. 2003. Effect of the type of environmental stress on the emergence of sunflower (Helianthus annus L.), soybean (Glycine max L.) and maize (Zea mays L.) seeds with different levels of vigor. Seed Science and Technology. 31:465-479. (Journal) 10. Dornbos, D.L., Mullen, R.E. and Shibles, R.M. 1989. Drought stress effects during seed filling on soybean seed germination and vigour. Crop Science. 29: 476-480. (Journal) 11. Ellis, R.H. and Raberts, E.H. 1980. Towards a rational basis for testing seed quality. In: P.D. Hebblethwaite (ed.) Seed Production. Bufferworths, London. pp. 605-635. (Journal) 12. Farooq, M., Basra, S.M.A., Wahid, A. and Khan, B. 2006. Rice seed invigoration by hormonal andvitamin priming. Seed Science and Technology. 34:775-780. (Journal) 13. Farooq, M., Wahid, A., Ahmadand, N. and Asad, S. 2010. Comparative efficacy surface drying and re-drying seed priming in rice. Change in emergence, seedling growth and associated metabolic events. Paddy Water Environ. 8: 15-22. (Journal) 14. Ghahremani, S., Sedghi, M. and Seyed Sharifi, R. 2017. Effect of seed deterioration on the activity of antioxidant enzymes and lipid peroxidation in Pumpkin (Cucurbita pepo L.) seedlings under different temperatures. Iranian Journal of Seed Science and Technology. 1: 205-218. (In Persian) (Journal) 15. Ghahremani, S., Sedghi, M. and Tavakoli, H. 2016. Effect of Gibberellin and Salicylic acid on the germination traits and activity of antioxidant enzymes in deteriorated Pumpkin seeds. Journal of Seed Research. 2: 20-29. (In Persian) (Journal) 16. Ghasemi Golezani, K., Salehian, V., Rahimzadeh Khoie, F. and Moghaddam, M. 1996. Effect of seed vigor on seedling emergence and grain yield of wheat. Journal of Agricultural Sciences and Natural Resources. 3: 48-54. (Journal) 17. Ghassemi-Golezani, K., Khomari, S., Dalili, B., Hosseinzade-Mahootchy, A. and Chadordooz-Jeddi, A. 2010. Effects of seed aging on field performance of winter oilseed rape. Journal Food Agricultural and Environmental Science. 8: 175-178. (Journal) 18. Goel, A. and Sheoran, I. S. 2003. Lipid peroxidation and peroxide-scavenging enzymes in cotton Seed under natural ageing. Biologia Plantarum. 46(3): 429-43. (Journal) 19. Gonzales, A., Gordan, A.J., James, C.L. and Arrese-Igor, C. 1995. The role of sucrose synthase in response of soybean nodules to drought. J. Expt. Bot. 46: 1515-1523. (Journal) 20. Hasan, M.A., Ahmed, J.U., Hossein, M.M. and Ullah, M.A. 2004. Germination characters and seed reserve mobilization during germination of different wheat genotypes under variable temperature regimes. Journal of the National Science Foundation of Sri Lanka. 32:97-107. (Journal) 21. International Seed Testing Association. 2008. International rules for seed testing (supplement). Seed Science and Technology. 27: 1-333. (Journal) 22. International Seed Testing Association (ISTA). 2012. International Rules for Seed Testing, 2012 edn. Bassersdorf, Switzerland: The International Seed testing Association (ISTA). 23. Kaur, S., Gupta, A.K. and Kaur, N. 2002. Effect of osmo and hydro priming of chickpea seeds onseedling growth and carbohydrate metabolism under water deficit stress. Plant Growth Regulation. 37: 17-22. (Journal) 24. Magbanua, Z.V., Moraes, C.M.D., Brooks, T.D., Williams, W.P. and Luthe, D.S. 2007. Is Catalase Activity One of the Factors Associated with Maize Resistance to Aspergillus flavus. Molecular Plant Microbe Interact. 20(6): 697–706. (Journal) 25. McDonald, M.B. 1999. Seed deterioration: Physiology, repair and assessment. Seed Science and Technology. 27: 177-237. (Journal) 26. Miguel, A., Rosales, Z., Juan, M., Ruiz, A., Hernandez, J., Soriano, T., Castilla, N. and Romero, L. 2006. Antioxidant content and as ascorbate metabolism in cherry tomato exocarp in relation to temperature and solar radiation. Journal of the Science of Food and Agriculture. 86: 1545-1551. (Journal) 27. Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science 7: 405-410. (Journal) 28. Mohammadi, H., Soltani, A., Sadeghipour, H.R., and Zeinali, E. 2011. Effects of seed aging on subsequent seed reserve utilization and seedling growth in soybean. International Journal of Plant Production, 5: 65-70. (Journal) 29. Noctor, G. and Foyer, G.H. 1998. Ascorbate and glutathione: keeping active oxygen under control. Annual review of plant physiology and plant molecular biology. 49:249–279. (Journal) 30. Perry, D.A. 1980. Seed vigor and seedling establishment. Advanced Research Technology of Seeds. 5:25-40. (Journal) 31. Powell, A.A. 1998. Seed improvement by selection and invigoration. Scientia Agricola Piracicaba. 55:126-133. (Journal) 32. Ram, C. and Weisher, L.E. 1988. Effect of artificial aging on physiological biochemical parameters of seed quality in wheat. Seed Science and Technology. 16:579-587. (Journal) 33. Rezaei, M., Sedghi, M. and Seyed Sharifi, R. 2014. Effect of seed priming on reserve mobilization of Pot marigold (Calendula officinalis L.) seeds under salinity stress. Journal of Research in Agricultural Systems. 2: 55-62. (In Persian) (Journal) 34. Saberi, M. and Tavili, A. 2010. Evaluation of different priming treatments influences on Puccinellia distans germination characteristics. Iranian Journal of Range and Desert Reseach, 17: 25-31. (In Persian) (Journal) 35. Sairam, R.K., Rao, K.V. and Srivastava, G.C. 2002.Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Science. 163:1037-1046. (Journal) 36. Sanchez, J.A., Munoz, B.C. and Fresneda, J. 2001. Combine effects of hydration-dehydration and heat shock treatments on the germination of tomato, pepper and cucumber. Seed Science and Technology. 29: 691-697. (Journal) 37. Sedghi, M., Khomari, S., and Amanpour-Balaneji, B. 2011. Effect of Seed Vigor and Hormone Priming on Glyoxylate Cycle Enzymes Activity in Persian Silk Tree (Albiziajulibrissin Durazz.). World Applied Science Journal. 13(3): 541-544. (Journal) 38. Sedghi, M., Nemati, A., and Esmaielpour, B. 2010. Effect of seed priming on germination and seedling growth of two medicinal plants under salinity. Emirates Journal of Food and Agriculture. 22(2): 130-139. (Journal) 39. Smirnoff, N. 2000. Ascorbic acid: metabolism and functions of a multi-facetted molecule. Current Opinion in Plant Biology. 3:229–235. (Journal) 40. Sofo, A., Tuzio, A.C., Dichio, B. and Xiloyannis, C. 2005. Influence of water deficit and rewatering on the components of the ascorbate-glutathione cycle in four interspecific Prunus hybrids. Plant Science. 169: 403-412. (Journal) 41. Soltani, A., Gholipoor, M., and Zeinali, E. 2008. Seed reserve utilization and seedling growth of wheat as affected by drought and salinity. Environmental and Experimental Botany. 55:195-200. (Journal) 42. Soltani, A., Zeinali, E., galashi, S., and Latifi, N. 2001. Genetic variation for and interrelationships among seed vigor traits in wheat from the Caspian Sea Coast of Iran. Seed Science and Technology. 29: 653-662. (Journal) 43. Tavakkoli Kakhki, H. R., Beheshti, A. and Nassiri Mahalati, M. 2005. Evaluation of seed vigor tests for determining alfalfa seed quality. Iranian Journal of Field Crops Research. 3(1): 25-34. (In Persian) (Journal)