تأثیر پرایمینگ بر جوانه‌زنی و ویژگی‌های گیاهچه در بذور فرسوده کدو پوست کاغذی تحت شرایط تنش خشکی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری دانشگاه محقق اردبیلی

2 استاد گروه تولید و ژنتیک گیاهی دانشگاه محقق اردبیلی

10.22124/jms.2022.6150

چکیده

 به­منظور بررسی اثر پرایمینگ بر جوانه­زنی بذور فرسوده کدو پوست کاغذی تحت تنش خشکی، آزمایشی به­صورت  فاکتوریل در قالب طرح کامل تصادفی با 3 تکرار در آزمایشگاه اجرا شد. تیمارها شامل فرسودگی (شاهد، 85 درصد و 75 درصد)، تنش خشکی (صفر، 75/0- و 5/1- مگاپاسکال) و پرایمینگ (شاهد، هیدروپرایمینگ، پرایمینگ با جیرلین، پرایمینگ با Gr24، پرایمینگ با بنزیل­آمینوپورین و پرایمینگ با اسپرمیدین) بودند. در این پژوهش صفات درصد جوانه­زنی، سرعت جوانه­زنی، یکنواختی جوانه­زنی، وزن خشک ساقه­چه و ریشه­چه، محتوای کلروفیل a و b، نسبت کلروفیل a/b، فتوسنتز، محتوای قندهای محلول، درصد چربی کل و فعالیت آنزیم آلفاآمیلاز مورد مطالعه قرار گرفت. کم­ترین درصد جوانه­زنی، سرعت جوانه­زنی، وزن خشک ریشه­چه و ساقه­چه، کلروفیل a، کلروفیل b، نسبت کلروفیل a/b، شدت فتوسنتز و فعالیت آنزیم آلفاآمیلاز در تیمار خشکی 5/1- مگاپاسکال و فرسودگی 75 درصد بود. کم­ترین یکنواختی جوانه­زنی در تیمار هورمون پرایمینگ با جیبرلین در شرایط عدم تنش به­دست آمد. کم­ترین محتوای قندها و درصد چربی کل در سطح شاهد (بدون تنش خشکی)، فرسودگی 75 درصد و در شرایط عدم پرایمینگ بود. بیشترین درصد جوانه­زنی، سرعت جوانه­زنی، وزن خشک ریشه­چه و ساقه­چه و فعالیت آنزیم آلفاآمیلاز در تیمار هورمون پرایمینگ با جیبرلین، کلروفیل a، کلروفیل b و شدت فتوسنتز در پرایمینگ بذر با اسپرمیدین و نسبت کلروفیل a/b در هیدروپرایمینگ و در شرایط عدم تنش خشکی و فرسودگی به­دست آمد. بیشترین یکنواختی جوانه­زنی در خشکی 5/1- مگاپاسکال و فرسودگی 75 درصد در پرایمینگ شاهد به­دست آمد. بیشترین محتوای قندها در شرایط عدم فرسودگی، خشکی 75/0- مگاپاسکال و تیمار به­روش هیدروپرایمینگ به­دست آمد. بیشترین درصد چربی کل در خشکی 5/1- مگاپاسکال، عدم فرسودگی و در پرایمینگ با اسپرمیدین مشاهده شد. به­طور کلی اعمال تنش خشکی بر روی بذور فرسوده کدو پوست کاغذی منجر به اختلال و یا کاهش در صفات فیزیولوژیک گردید. از طرفی پرایمینگ بذور با روش­های مختلف اثرات سوء ناشی از تنش خشکی و فرسودگی بذور را را تقلیل بخشید.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Seed Priming on Germination and Seedling Characteristics of Aged Squash Seeds under Drought Stress

نویسندگان [English]

  • Parisa Sheikhnavaz Jahed 1
  • Mohammad Sedghi 2
  • Raouf Seyed Sharifi 2
  • Omid Sofalian 2
1 1. Ph.D. Student, University of Mohaghegh Ardabili
2 2. Professor Of the Department of Plant Production and Genetics, University of Mohaghegh Ardabili
چکیده [English]

In order to investigate the effect of priming on different aspects of germination of aged pumpkin seeds under drought stress, a factorial experiment was conducted based on a completely randomized design with four replications in the laboratory. Treatments included different levels of aging (control, 85% and 75% of control germination), drought stress (0, -0.75 and -1.5 MPa), and priming with 100 μM of (control, hydro, gibberellin, GR24, benzyl aminopurine and spermidine). The seeds reached the desired vigor level by accelerated ageing test. Results of mean comparison showed that the lowest germination rate (2.11), fresh weight of radical (0.075 g), fresh weight of plumule (0.123 g), dry weight of radical (0.0082 g), dry weight of plumule (0.0079 g), chlorophyll a (0.403 mg.g-1FW), chlorophyll b (0.43 mg.g-1FW), chlorophyll a/b (0.99), photosynthesis 0.723 µmolCO2.mm-1.s-1) and α-amylase activity (12.37 μgSturch.g-1.minFW) were related to drought treatment with an intensity of -1.5 MPa and with 75% aging in the absence of priming. The lowest of germination uniformity (0.97) was obtained in the absence of drought and aging in priming with gibberllin. The lowest amount of carbohydrates (354.33 µmol.g-1FW) and total oil percentage (11.16) were obtained in the absence of drought and priming with 75% aging. The highest germination rate (15.56) in hormone-priming with gibberllin, fresh weight of radical (0.255 g) in priming with hydropriming, fresh weight of plumule (0.305 g) in priming with hydropriming, dry weight of radical (0.014 g) in hormone-priming with gibberllin, dry weight of plumule (0.0188 g) in priming with gibberllin, chlorophyll a (1.876 mg.g-1FW) in priming with spermimin, chlorophyll b (0.76 mg.g-1FW) in priming with spermimin, chlorophyll a/b (0.99) in priming with hydropriming, photosynthesis (5.833 µmolCO2.mm-1.s-1) in priming with spermimin and α-amylase activity (66.4 μgSturch.g-1.minFW) in hormone-priming with gibberlin were obtained in the absence of drought and aging stress. The highest germination uniformity (4.87) was related to drought treatment with an intensity of -1.5 MPa and with 75% aging in the absence of priming. The highest amount of carbohydrate (1561.33 µmol.g-1FW) was related to drought treatment with an intensity of -0.75 MPa and in the absence of aging and with of priming hydropriming. The highest amount of total oil percentage (21.2) was related to drought treatment with an intensity of -1.5 MPa, absence of ageing and priming with spermidin.

کلیدواژه‌ها [English]

  • fresh weight
  • growth traits
  • hormones
  • seedlings
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