بررسی تاثیر انبارداری و روش‌های مختلف پرایم نمودن بر صفات جوانه زنی نمونه‌های بذر گونهHalocnemum strobilaceum موجود در بانک ژن منابع طبیعی ایران

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دکترای تخصصی، موسسه تحقیقات جنگل‌ها و مراتع کشور، تهران، ایران

2 کارشناسی ارشد، موسسه تحقیقات جنگل‌ها و مراتع کشور، تهران، ایران

10.22124/jms.2022.6148

چکیده

سرعت زوال بذر و تاثیر روش‌های مختلف پرایم بر بهبود جوانه‌زنی بذور اکسشن‌های مختلف بذر گیاه Halocnemum strobilaceum  موجود در بانک ژن منابع طبیعی ایران در این آزمایش بررسی شد. برای بررسی سرعت زوال بذور، آزمون متداول جوانه‌‌زنی بر روی 42 اکسشن متفاوت از نظر محل و زمان جمع‌آوری، انجام گردید. تاثیر روش‌های مختلف پرایم شامل تیمارهای اسید جیبرلیک در دو سطح 250 و 500 میلی‌گرم در لیتر، پلی‌اتیلن‌گلایکول در دو سطح 6/0 و2/1 مگاپاسکال، نیترات‌پتاسیم با غلظت 78/19 میلی­مولار، سرمادهی مرطوب در دمای چهار درجه سلسیوس، به­مدت یک ماه، کلرید‌سدیم با غلظت 100 میلی­مولار بر روی سه اکسشن 1645، 33515 و 43882 (به­ترتیب 17، 9 و 5 سال در سردخانه نگهداری شده بودند) آزمایش شد. نتایج نشان داد میانگین سرعت زوال بذور 53/3 درصد به ازاء هر سال نگهداری در دمای چهار درجه سلسیوس می­باشد. همچنین واکنش بذور هر اکسشن به تیمارهای مختلف پرایم متفاوت است. به­طوری­که اکسشن 1645 بر اثر تیمار کلرید‌سدیم با غلظت 100 میلی­مولار و سرمادهی، اکسشن 33515 با تیمار اسید جیبرلیک با غلظت 500 میلی‌گرم در لیتر و اکسشن 43882 در شاهد، درصد جوانه‌زنی بیش­تری داشتند. در صفت طول ریشه­چه و ساقه­چه هیچ تفاوت معنی‌داری بین تیمارها مشاهده نشد. از نتایج این آزمایش می­توان استدلال نمود که تیمارهای پرایم فقط می­تواند توانایی ذاتی بذر را به مرحله ظهور برساند. لذا برای جمع‌آوری و ذخیره بذور برای مدت طولانی سلامت و کیفیت اولیه بذر باید مورد توجه قرار گیرد. 

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of the effect of storage and different priming methods on germination traits on Halocnemum strobilaceum accessions exist in the Natural Resources Gene Bank of Iran

نویسندگان [English]

  • Mohammad Dadmand 1
  • Parvin Salehi Shanjani 1
  • Leila Rasoolzadeh 2
  • Mostafa Assadi 1
  • Leila Falah Hoseini 2
1 Research Institute of Forest and Rangelands, Tehran, Iran
2 Research Institute of Forest and Rangelands, Tehran, Iran
چکیده [English]

To evaluate the deterioration rate and effect of different priming methods on improving seed germination on different seed accessions of Halocnemum strobilaceum provided by Natural Resources Gene Bank of Iran, To evaluate the deterioration rate, a standard germination test was performed on 42 seed accessions with different origin and collection dates. To study effect of different priming methods, treatments included gibberellic acid (250 and 500 mg/l), polyethylene glycol (-0.6 and -1.2 MPa), potassium nitrate 19.78 mM, stratification at 4 °C for one month, NaCl 100 mM and Chilling were compared in 1645, 33515 and 43882 accessions That5, 9 and 17 years were stored in the refrigerator. The results showed that the average seed deterioration rate was 3.53 %/y of stored seeds at 4 °C.Each seed accession showed a different reaction to prime treatment. Results indicated germination percentage improved in the accession 1645 by treatment NaCl 100 mM, in the accession 33515 by treatment gibberellic acid 500 NaCl 100 mM, and in the accession 43882 by control treatment. However, no significant differences were observed between radicle and pedicle lengths among different treatments. From the results of this experiment, it can be concluded that priming treatments can only express the potential of the seed. Therefore, for collecting and storing seeds for a long time, the health and initial quality of seeds are very important.

کلیدواژه‌ها [English]

  • Accessions
  • Deterioration
  • Halocnemum strobilaceum
  • Priming
Akhani, H. 2004. Halophytic vegetation of Iran: Towards a syntaxonomical classification. Annual Botany, 4: 66–82. (Journal) Alam, M.M., Hasanuzzaman, M., Nahar, K. and Fujita, M. 2013. Exogenus salcilyc acid ameliorate short-time brought stress in mustard (Brassica juncea) seedling by upregulating the antioxidant defense and glyoxalas system. Australian Journal of Crop Science, 7:1053-1063. (Journal) Alipour, S., Tehranifar, A., Shoor, M., Samiei, L. and Farahmand, H. 2019. Role of stratification and priming with arginine and gibberellic acid on germination Character’s of Fritillaria raddeana .Iranian Journal of Seed Science and Technology, 8(1): 19-30. (In Persian)(Journal) Allah-Dadi, M. 2020. Effects of maternal plant nutrition on some seed germination characteristics andseedling growth of artichoke (Cynara scolymus L.). Iranian Journal of Seed Science and Technology, 8(2): 59-72. (In Persian)(Journal) Amiri, M.B., Rezvani Moghaddam, P. and Jahan. M. 2018. Evaluation of Germination Characteristics and Seeling Growth of Iranian Ox-Tongue (Echium amoenum Fisch and Mey.) Seed Resulting from the Rootstock treatmented by Biological and Chemical Fertilizers in Different Planting Dates and Methods. Iranian Journal of Seed Science and Technology, 7(1): 181-199. (In Persian)(Journal) Conrath, U. 2011. Molecular aspects of defense priming. Trends in Plant Science, 16(10): 524-531. doi:10.1016/j.tplants.2011.06.004. Copland, L.O. and McDonald, M.B. 1995. Principles of Seed Science and Technoloy. Thired edition. Champan and Hall, 393p. (Book) Delouche, J. 1973. Precepts of seed storage. Proceeding of the Mississsippi State. Seed proceessors Shortcourse, 12: 93-122. (Journal) Ghadamyari, Sh., Mozafari, J., Sokhandan, N., Bashir Mosavi, L. and Rakhshanderoo, F, 2011. Synergistic effects of mechanical and chemical treatments on seed germination of Jimson weed (Datura stramonium L.). Iranian Journal. Biology, 24(6): 809-817. (In Persian)(Journal) Ghasemi Phirouzabadi, A.A., Jafari, M., Heidari sharifabad, H., Azarnivand, H. and Abbasi H.R. 2009. Investigation of the morphologic- physiologic changes of puccinelia distance and Aeluropus littoralis to salinity and drought resistance. Iranian journal of Range and Desert Reseach, 16(1): 1-10. (In Persian)(Journal) Hamidi, A., Rudi, D., Asgari, V. and Hajilui, S. 2009. Study on applicability of controlled deterioration vigour test for evaluation of seed vigour and field performance of three oil-seed rape (Brassica napus L.) cultivars. Journal of Plant and Seed, 24: 677-706. (In Persian)(Journal) Harris, D., Raghumanshi, B.S., Gangwar, J.S., Singh, S.C., Joshi, K.D., Rashid, A. and Hollington P.A. 2001. Participatory evaluation by farmers of on-farm seed priming in wheat in India, Nepal and Pakistan. Experimental Agriculture, 37: 403-415. (Journal) Huang, Z.Y. and Gutterman, Y. 2000. Comparison of germination strategies of Artemisia ordosica with its two congeners from deserts of China. Acta Botanica Sinica, 42: 71–80. (Journal) Hunter, E.A., Glasbey, C.A. and Naylor, R.E.L. 1984. The analysis of data from germination tests. Journal of Agricultural Science. Cambridge, 102: 207-213. (Journal) ISTA, 2017. International Seed Testing Association. International Rules for Seed Testing. Seed Science and Technology.www. ISTA.com Khajeh-Hosseini, M., Rashed-Mohassel, M.H., Mahmoodi, P. and. Emamipoor, Y. 2018. Investigation on the germination characteristics and seed dormancy of fourteen medicinal plant species (Lamiaceae family) grown in Kerman Province. Iran Iranian Journal of Seed Science and Technology, 7(1): 233-242. (In Persian)(Journal) Kibinza, S., Bazin, J., Bailly, C., Farrant, J.M., Corbineau, F. and El-Maarouf-Bouteau, H. 2011. Catalase is a key enzyme in seed recovery from ageing during priming. Plant Science, 181: 309-315. (Journal) Kibinza, S., Vinel, D., Come, D., Bailly, C. and Corbineau, F. 2006. Sunflower seed deterioration as related to moisture content during ageing, energy metabolism and active oxygen species scavenging. Plant Physiology, 128: 496-506. (Journal)) Liu, YX. 1985. Flora in desertis reipublicae popularum sinarum.Volume I. Beijing: Science Press, 3:15-27. (In Chinese) (Journal)) Makkizadeh Tafti, M., Farhoudi, A., Rastifar, M.A. and. Sadat Asilan, K. 2012. Methods of breaking dormancy in Caper (Capparis spinosa L.). Iranian Journal Range Desert Research, 18(4): 569-577. (In Persian)(Journal) Nemati, D., Sharifi, H., Urdkaneh, M., Sharifi, Z., 2002. Effect of moist chilling and gibberellic acid on seed dormancy in two species of Silybum mrianum and Citrulus colocynthis. Seed Research, 3(1):169-177. (In Persian)(Journal) Pessarakli, M. 1999.Hand book of Plant and Crop Stress. Marcel Dekker Inc, 679 p. (Book) Pessarakli, M., Marcum, K.B. and. Kopec, D.M. 2001. Growth Responses of Desert Saltgrass under Salt Stress. Turfgrass Landscape and Urban IPM Research Summary 2001, Cooperative Extension, Agricultural Experiment Station, The University of Arizona, Tucson, U.S. Department of Agriculture. AZ1246 Series P-126, pp. 70-73. (Journal) Reddy, K.N. and Singh, M. 1992. Germination and emergence of hairy beggarticks (Bidens pilosa). Weed Science, 40:195–199. (Journal) Rezaee, M. 2011. Effect of environmental conditions on growth and germination characteristics of Amaranthus retroflexus L., Solanum nigrum L. and Daturea stramonium L. seeds resulting from the rootstock. M.S. Thesis in Birjand University. Iran. (Thesis) Saghafi Kenari, F., Hamidi, A., Mobasser, H. and Nabi Ilkaee, M. 2020. Effect of seed moisture content and storage duration on three wheat (Triticum aestivum L.) cultivars germination and early seedling growth in Mazandaran province. Iranian Journal of Seed Science and Research, 6(4):539-555. (In Persian)(Journal) Salehzade, H., Izadkhah Shishvan, M. and Chiyasi, M. 2009. Effect of seed priming on germination and seedlinggrowth of wheat (Triticum aestivum L.). Journal of Biological Sciences, 4(5): 629-631. (Journal) Scott, S.J., Jones, R.A. and Williams, W.A. 1984. Review of data analysis methods for seed germination. Journal of Crop Science, 24: 1192-1199. (Journal) Sheidaei, S., Hamidi, A., Sadeghi, H. and Oskouei, B. 2020 Evaluation of initial seed quality and storage conditions on biochemical and physiological changes of soybean seeds Iranian Journal of Seed Science and Technology. 9(2): 101-118. (In Persian)(Journal) Sheidaei, S., Heidari Sharisabad, H., Hamidi, A., Noormohammadi, G. and Moghaddam, A. 2016. Effect of storage condition, initial seed moisture content and germination on soybean seed deterioration. Iranian Journal of Seed Research, 2(2): 31-47. (In Persian)(Journal) Sivritepe, H.Ö., Sivritepe, N., Eriş, A. and Turhan, E. 2005. The Effects of NaCl Pre-treatments on Salt Tolerance of Melons Grown under Long-term Salinity. Science. Horticalture, 106: 568-581. (Journal) Souri, M.K., Naiji, M. and. Aslani, M. 2018. Effect of Fe-Glycine Aminochelate on pod quality and Iron concentrations of Bean (Phaseolus vulgaris L.) under lime soil conditions. Communication Soil Science Plant Analysis, 4(2): 215-224. (Journal) Yadav, R., Singh, S.S., Jain, N., Singh, G.P. and Prabhu, K.V. 2010. Wheat production in India: Technologies to face future challenges. Journal of Agricultural Science, 2(2): 164-174. (Journal)