شناسایی QTL‌های مرتبط با مولفه‌های جوانه‌زنی جو (Hordeum vulgare L) در شرایط نرمال، تنش خشکی و شوری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی ارشد بیوتکنولوژی، گروه تولیدات گیاهی، دانشکده علوم کشاورزی و منابع طبیعی، دانشگاه گنبد کاووس، ایران

2 دانشیار گروه تولیدات گیاهی، دانشکده علوم کشاورزی و منابع طبیعی، دانشگاه گنبد کاووس، ایران

3 استادیار گروه تولیدات گیاهی، دانشکده علوم کشاورزی و منابع طبیعی، دانشگاه گنبد کاووس، ایران

4 گروه اصلاح نباتات و منابع ژنتیکی، اگروسکوپ، سوئیس

10.22124/jms.2022.6145

چکیده

جوانه­زنی بذر از خصوصیات بارز و مهم یک رقم زراعی توصیف می­شود. به­منظور شناساییQTLهای مرتبط با جوانه­زنی جو تحت شرایط نرمال، شوری و خشکی، 103 خانواده F8 حاصل از تلاقی دو رقم بادیا×کویر در قالب طرح کاملا تصادفی در سال 1398-1399 در آزمایشگاه گیاه­شناسی دانشگاه گنبدکاووس در دو تکرار بررسی شدند. تعداد ریشه­چه، طول ریشه­چه، وزن ریشه­چه، طول کلئوپتیل، طول ساقه­چه، وزن ساقه­چه و درصد جوانه­زنی مورد اندازه­گیری قرار گرفتند. نقشه پیوستگی بر اساس 152 آلل چند شکل SSR، 72 آللISSR ، 7 آلل IRAP، 29 آللCAAT ، 27 آلل Scot و 15 آلل iPBS تهیه شد. نشانگرهای مولکولی استفاده شده هفت کروموزوم جو با طول نقشه 2/999 سانتی­مورگان منتسب شد. میانگین فاصله بین دو نشانگر مجاور برابر 387/3 سانتی­مورگان شد. تجزیه QTL به­روش نقشه­یابی فاصله­ای مرکب (CIM) برای هر صفت در هر محیط انجام شد. در این پژوهش در هر سه شرایط کروموزوم­های 4، 5 و 7 به­دلیل قرارگرفتن بیش­ترین­QTLها به­عنوان مهم­ترین کروموزوم­ها بودند. در شرایط نرمال دوQTL  بزرگ اثر برای طول کلئوپتیل (qCLN-5a) و درصد جوانه­زنی (qGPN-4b) ردیابی شد، در شرایط تنش خشکی سهQTL  بزرگ اثر برای طول ریشه (qRLD-4b) و (qRLD-5) برای وزن ساقه­چه (qPWD-4) ردیابی شد. از نتایج این پژوهش بعد از تعیین اعتبار می­توان در برنامه­های انتخاب به کمک نشانگر استفاده نمود.

کلیدواژه‌ها


عنوان مقاله [English]

Identification of QTLs related to germination parameters in barley (Hordeum vulgare) under normal, drought and salinity condition

نویسندگان [English]

  • Somayyeh Makhtoom 1
  • Hosein Sabouri 2
  • Abdollatif Gholizadeh 3
  • Leila Ahangar 3
  • Mahnaz Katoozi 4
1 MSc student of Biotechnology, University of Gonbad Kavoos
2 Associate Professor, University of Gonbad Kavoos
3 Assistant Professor, University of Gonbad Kavoos
4 Agroscope, Switzerland
چکیده [English]

Seed germination is described as a prominent and important feature of a cultivar. In order to identify QTLs related to barley germination under normal, salinity and drought conditions, 103 F8 families from Crosses of two cultivars, Badia × Kavir, were assessed using completely randomized design in two replications during the 2018-2019, in the botanical laboratory of Gonbad Kavous University, Iran. Number of roots, root length, root weight, coleoptile length, plumule length, plumule weight and germination percentage. Linkage maps were prepared using 152 SSR polymorphic markers, 72 ISSR alleles, 7 IRAP alleles, 29 CAAT alleles, 27 Scot alleles and 15 iPBS alleles. The molecular markers used were attributed to 7 barley chromosomes with a map length of 999.2 centi Morgans (cM). The mean distance between two adjacent markers was 3.387 cM. QTL analysis was performed using composite distance mapping (CIM) for each trait in each environment. In this study, chromosomes 4, 5 and 7 were the most important chromosomes in all three conditions due to the presence of the most QTLs. Under normal conditions 2 major effect QTLs were detected for the coleoptile length (qCLN-5a) and Germination percentage (qGPN-4b) was detected, Under drought stress conditions, 3 major effect QTLs were detected for root length (qRLD-4b) and (qRLD-5) for stem weight (qPWD-4). The results of this research can be used in marker assisted selection programs after determining the validity

کلیدواژه‌ها [English]

  • Barley
  • Germination
  • Salinity and drought stress
  • Marker selection
  • Molecular markers
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