بررسی سیستم آنتی‌اکسیدانت غیرآنزیمی، کمیت پروتئین و روغن در شرایط پیری تسریع‌شده بذرهای دو رقم کلزا (Brassica napus L.)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی‌ارشد علوم و تکنولوژی بذر، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

2 استاد گروه اگروتکنولوژی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

3 استادیار پژوهشکده علوم گیاهی، دانشگاه فردوسی مشهد، مشهد، ایران

4 استادیار گروه بیوتکنولوژی وبه نژادی گیاهی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

10.22124/jms.2022.6141

چکیده

این پژوهش با هدف بررسی اثر پیری تسریع­شده (AA) بر شاخص‌های فیزیولوژیک و بیوشیمیایی بذر کلزا در قالب آزمایش فاکتوریل بر مبنای طرح کاملاً تصادفی با دو عامل رقم (مودنا و اوکاپی)، سطح پیری (صفر، 24 و 72 ساعت دمای 40 درجه سلسیوس و رطوبت اشباع) و چهار تکرار در سال 99-1398 در دانشگاه فردوسی مشهد انجام شد. نتایج نشان داد که بذرهای پیر شده ارقام اوکاپی و مودنا، کاهش معنی‌داری در شاخص‌های جوانه‌زنی داشتند. در تیمار 72 ساعت پیری در مقایسه با شاهد، محتوای روغن، پروتئین و فعالیت مهار آنتی­اکسیدانت، به­ترتیب، 89/9، 14/4 و 61/28 درصد کاهش یافت. اما نشت الکترولیت‌ها 64/84 درصد نسبت به شاهد افزایش یافت. پیری، محتوای آنتی‌اکسیدانت‌های غیر آنزیمی را نیز تحت تأثیر قرار داد. در رقم اوکاپی، فلاونوئیدها 79/73 درصد، پرولین 15/47 درصد و فنل 13/17 درصد در مقایسه با بذرهای پیر نشده کاهش یافت. رقم اوکاپی، هر چند دارای خصوصیات کیفی مانند درصد روغن، فعالیت سیستم‌های آنتی‌اکسیدانت بالاتری بود، اما به انبارداری بسیار حساس بود، چرا‌ که پیری باعث افت شدید کیفیت بذرهای رقم اوکاپی شد. بنابراین برای جلوگیری از خسارت‌های اقتصادی، باید به شرایط محیطی نگهداری بذرهای این رقم، توجه بیش‌تری گردد. به­طورکلی، کنترل شرایط انبارداری برای به حداقل رساندن پیری و زوال بذر کلزا، امری حیاتی است.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of non-enzymatic antioxidant system, quantity of protein and oil of two canola cultivars (Brassica napus L.) seeds during accelerated aging conditions

نویسندگان [English]

  • Maryam Zamanian 1
  • Reza Tavakkol Afshari 2
  • Jafar Nabati 3
  • Alireza Seifi 4
1 Master in Seed Science and Technology, Department of Agrotechnology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
2 Professor, Department of Agrotechnology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
3 Assistant Professor of Crop Physiology, Research Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
4 Assistant Professor, Department of Crop Biotechnology and Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
چکیده [English]

In order to investigate the effect of accelerated ageing (AA) on physiological and biochemical traits of cultivar canola seeds, this study was laid out as a factorial experiments based on a completely randomized design with two factors cultivars (Modena and Okapi), aging level (0, 24 and 72 hours at temperature of 40˚c and relative humidity of saturated) and four replications in the Ferdowsi University of Mashhad at 2019-20. The results showed that the aged seeds of Okapi and Modena cultivars significant decline in germination characteristics. According to the biochemical results, in the treatments of 72 hours of AA compared to the control, oil, total protein and scavenging free radicals of canola seeds, has decreased by 9.89, 4.14 and 28.61 percent, respectively. Likewise, electrical conductivity increased by 84.64 percent. Interestingly, aging affected the content of non-enzymatic antioxidants. Here for example, it reduced flavonoids by 73.79%, proline by 47.18%, and phenol by 17.13% compared to non-aged seeds in Okapi cultivar. On the one hand, Okapi cultivar had great quality characteristics such as oil percentage, activity of antioxidant systems, but on the other hand, it was very sensitive to storage, because, aging caused extremely decline in Okapi cultivar seed quality. So, it is obvious that conditions of seed storage of this cultivar require more attention. In general, controlling storage conditions to minimize canola seed aging and deterioration is critical.

کلیدواژه‌ها [English]

  • Flavonoid
  • Modena
  • Okapi
  • Phenol
  • Proline
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