آنالیز پروتیوم بذور گندم تحت تنش خشکی شدید و احیاء

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار پژوهشی بخش فیزیولوژی مولکولی، پژوهشکده بیوتکنولوژی کشاورزی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

2 کارشناس بخش زیست‌شناسی سامانه، پژوهشکده بیوتکنولوژی کشاورزی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

چکیده

گیاهچه‌های گندم بهاره تحمل زیادی نسبت به تنش شدید خشکی تا روز چهارم پس از جوانه‌زنی از خود نشان می‌دهند. تحقیق حاضر به­منظور مطالعه پروتئین‌های پاسخ­دهنده به تنش در بذور متصل به گیاهچه‌های 4 روزه گندم در شرایط تنش خشکی و آبیاری مجدد می­باشد. آزمایش با اعمال تنش خشکی با قطع کامل آبیاری در دو دوره ده و بیست روزه و آبیاری مجدد به­مدت سه و هفت روز در اتاقک رشد در قالب یک طرح کاملاً تصادفی با سه تکرار زیستی انجام گرفت. بذور متصل به گیاهچه برای سنجش فیزیولوژیک و پروتیوم مورد بررسی قرار گرفتند. بررسی صفات فیزیولوژیک به­خوبی شدت تنش و تاثیر تنش بر بذر را نشان داد و با آبیاری مجدد نیز در تمامی آزمایشات بذر به شرایط شاهد بسیار نزدیک شد. بررسی پروتئوم با الکتروفورز دوبعدی در بذر تمامی تیمارها انجام و657 لکه پروتئینی با رنگ­آمیزی نیترات نقره مشاهده شدند. در نهایت پس از آنالیز معنی‌داری130 لکه انتخاب و پس از جستجو در بانک­های اطلاعاتی پروتئین، شناسایی شدند. تنش خشکی موجب افزایش بیان ژن‌های موثر در تولید پروتئین‌های دفاعی (افزایش پرولین)، شکسته­شدن ترکیبات ذخیره‌ای بذر و تولید پروتئین‌های موثر در تغییرات ساختمانی دیواره سلول شد. از طرف دیگر کاهش بیان پروتئین‌های موثر در تنفس و تجزیه پروتئین موجب کاهش مصرف انرژی در سلول در شرایط تنش شد. در شرایط آبیاری مجدد افزایش بیان پروتئین‌های موثر در متابولیسم کربوهیدرات‌ها موجب مصرف مواد غذایی موجود در بذر (کاهش وزن بذر) و تولید پروتئین‌های ساختمانی جدید (حضور برگ و ریشه جدید) شد.

کلیدواژه‌ها


عنوان مقاله [English]

Proteome analysis of wheat seeds under severe drought stress and recovery

نویسندگان [English]

  • Parisa Koobaz 1
  • Manzar Heidari 2
1 Research Assistant Prof. Department of Molecular Physiology, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education, and Extension Organization (AREEO), Karaj, Iran
2 Researcher, Department of Systems and Synthetic Biology, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education, and Extension Organization (AREEO), Karaj, Iran
چکیده [English]

Seedling wheat spirit are tolerant to strict dehydration up to the 4th day following imbibitions. The aim of present study is investigation of responsive proteins to drought stress and recovery in seeds attached to four day seedlings. The test was done by drought stress using interruption of irrigation in two time points (10 and 20 days) and recovery for 3 and 7 days in a growth chamber. All the experiments were conducted based on Completely Randomized Design (CRD) with three replications.Seedlings attached to seedlings were examined for physiological and protein assays. Physiological traits showed strict drought stress level on seed and recovery could return seedlings to normal condition. 2DE done on seed of all treatments and replicates and 657 protein spots were reproducibly detected by Ag Nostaining from seeds. Finally, after significance test, 130 spots were detected by searching protein data banks. Drought stress has increased the expression of genes involved in the production of defense proteins (proline increase), the breakdown ofseed storage compounds, and the production of proteins that are effective in structural changes in the cell wall. On the other hand, reduced expression of proteins effective in respiration and protein breakdown has reduced energy consumption in cells under stress. Under re-irrigation conditions, increased expression of proteins effective in carbohydrate metabolism has led to the consumption of nutrients in seeds (reduction of seed weight) and the production of new structural proteins (presence of new leaves and roots).

کلیدواژه‌ها [English]

  • Drought
  • Physiology
  • Proteome
  • Wheat seed
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