تأثیر پیش‌تیمارهای مختلف و تنش شوری بر جوانه‌زنی بذر و صفات کیفی گیاه دارویی شنبلیله (Trigonella foenum-graecum)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناسی ارشد علوم و تکنولوژی بذر، دانشکده کشاورزی، دانشگاه شاهد، تهران، ایران

2 دانشیار، دانشکده کشاورزی، دانشگاه شاهد، تهران، ایران

3 دانشجوی دکتری زراعت، دانشکده کشاورزی، دانشگاه شاهد، تهران، ایران

چکیده

به­منظور بررسی تاثیر پرایمینگ بر ویژگی­های جوانه­زنی بذر، رشد و صفات فیزیولوژیک گیاه دارویی شنبلیله تحت تنش شوری، آزمایشی به­صورت فاکتوریل در قالب طرح پایه کاملاً تصادفی با سه تکرار در آزمایشگاه تکنولوژی بذر دانشکده علوم کشاورزی دانشگاه شاهد در سال 1396 اجرا شد. تیمارهای آزمایش شامل 5 سطح (شاهد (بدون پرایم)، هیدروپرایمینگ آب مقطر به­مدت 24 ساعت، هیدروپرایمینگ آب مقطر به­مدت 48 ساعت، پرایم با نیترات پتاسیم سه درصد و سالیسیلیک اسید پنج درصد) و تنش شوری در چهار سطح (شاهد (صفر)، 50، 100 و 150 میلی­مولار) بود. نتایج تجزیه واریانس حاکی از تأثیر معنی­دار ترکیب تیماری پرایمینگ و تنش شوری بر صفات درصد و سرعت جوانه­زنی، میانگین مدت جوانه­زنی، شاخص طولی و وزنی بنیه گیاهچه، طول ریشه­چه و ساقه­چه، رنگیزه­های فتوسنتزی و محتوای پرولین بود. نتایج مقایسه میانگین اثر برهمکنش نشان داد که کاربرد هیدروپرایمینگ 24 ساعت در سطوح تنش شوری دارای میانگین 100 درصد جوانه­زنی بود. هیدروپرایمینگ 48 ساعت در صفاتی مانند طول ساقه­چه و ریشه­چه، شاخص طولی بنیه گیاهچه، محتوای کلروفیل، محتوای کارتنوئید و محتوای پرولین با افزایش تنش شوری نسبت به سطوح دیگر دارای برتری نسبی بود. به نظر می­رسد گیاه شنبلیله گیاهی مقاوم به تنش می­باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of different pre-treatments and salinity on seed germination and qualitative traits of )Trigonella foenum-graecum( medicinal plant

نویسندگان [English]

  • Zeynab Valipour Dehno 1
  • Majid Amini Dehghi 2
  • Shokofeh Gholami 3
1 In order to study priming on seed germination, growth and physiological characteristics of Fenugreek plant under salt stress, a factorial experiment was conducted in a completely randomized design with three replications at the Seed
2 Associate Professor, Faculty of Agriculture, University of Shahed, Tehran, Iran
3 Ph.D Candidate of Agronomy, Faculty of Agriculture, University of Shahed, Tehran, Iran
چکیده [English]

In order to study priming on seed germination, growth and physiological characteristics of Fenugreek plant under salt stress, a factorial experiment was conducted in a completely randomized design with three replications at the Seed Technology Laboratory Faculty of Agricultural Sciences Shahed University in year 2017. The treatments consisted of 5 levels of priming(control (no prime) and hydropriming distilled water for 24 hours, hydroperiming distilled water for 48 hours, 3% potassium nitrate and 5% salicylic acid) and Salinity stress in four levels (0, 50, 100 and 150 mM). The results of analysis of variance indicated significant effect of priming treatment and salinity stress on germination percentage and rate, rash parameters, photosynthetic pigments and proline content. The results of the comparison of the mean effect of interaction indicated that 24-hour hydroperimage application at salinity levels had an average of 100% germination percentage. Hydropriming had a superior advantage in terms of stem and root length, longitudinal seedling index, chlorophyll content, carotenoid content and prolin content with increasing salinity stress compared to other levels. The herbaceous plant seems to be resistant to stress. In general, the use of hydro-priming is recommended for modifying the stress of salinity in Fenugreek.

کلیدواژه‌ها [English]

  • Carotenoid
  • Chlorophyll
  • Indian Fenugreek
  • Proline
  • Seedling length
  • Weight Index
Abbasi, R., Esmailzadeh, I. and Zeynali, A. 2013. Effect of seed priming with salicylic acid and salinity levels on Fenugreek (Trigonella foenum-graecum). National Conference of Passive Defense In Agriculture. Qeshm Island – November, 2013. (Conference)(In Persian).
Arnon, D.I. 1949. Copper enzymes in isolated chloroplasts. Poly phenol oxidase in Beta vulgaris. Plant Physiology, 24(1): 1-150. (Journal)
Arzani, A. 2008. Improving salinity tolerance in crop plants: A biotechnological view. In Vitro Cellular and Developmental Biolo-Plant, 44: 373-383. (Journal)(In Persian)
Ardalani, H., Eradatmand Asli, D. and Moradi, P. 2014. Physiological and Morphological Response of Lemon Balm (Melissa officinalis L.) to Prime Application of Salicylic Hydroxamic Acid. Electronic Journal of Biology, 10(3): 93-97. (Journal)
Ashraf, M. and Foolad, M.R. 2005. Pre-sowing seed treatment-A shotgun approach to improve germination, plant growth, and crop yield under saline and none-saline conditions, Advances in Agronomy, 88: 223-271. (Journal)
Ashraf, M., Mukhtar, N., Rehman, S. and R.E.S. 2004. Salt-induced changes in photosynthetic activity and growth in a potential medicinal plant Bishop’s weed (Ammi majus L.). Photosynthetica, 42(4): 543-550. (Journal)
Ayub, M., Ibrahim, M., Noorka, I.R., Tahir, M., Tanveer, A. and Ullah, A. 2013. Effect of seed priming on seed germination and seedling growth of garden cress (Lepidium sativum L.). International Journal of Agriculture and Applied Sciences, 5: 1-5. (Journal)
Bates, L.S., Waldern, R.P. and Teave, I.D. 1973. Rapid determination of free proline for water stress studies. Plant and Soil, 39: 205-207. (Journal)
Cuin, T.A., Parsons, D. and Shabala, S. 2010. Wheat cultivars can be screened for NaCl salinity tolerance by measuring leaf chlorophyll content and shoot sap potassium. Functional Plant Biology, 37: 656-664. (Journal)
Demir Kaya, M., Gamze Okc¸ U., Atak, M. and Yakup, C. 2006. Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.), European Journal of Agronomy, 24: 291–295. (Journal)
Donaldson, E., Schillinger, W.F. and Stephen, M.D. 2001. Straw production and grain yield relationships in winter wheat. Crop Science, 41: 100 106. (Journal)
Ehsanzadeh, P., Nekoonam, M.S., Azhar, J.N., Pourhadian, H. and Shaydaee, S. 2009. Growth, chlorophyll, and cation concentration of tetraploid wheat on a solution high in sodium chloride salt: hulled versus free-threshing genotypes. Journal of Plant Nutrition, 32, 58-70. (Journal)(In Persian)
Ehyaii, H.R. and Khajeh Hosseini, M. 2011. Evaluation of germination and sleep characteristics in 30 seedlings of medicinal plants. Iran Agronomic Research, 9(4): 658-651. (Journal)(In Persian)
Ellis, R.H. and Roberts, E.H. 1981. The quantification of ageing and survival in orthodox seeds. Seed Science and Technology, 9: 377-409. (Journal)
Fariduddin, Q., Hayat, S. and Ahmad, A. 2003. Salicylic acid influences on net photosynthetic rate, carboxylation efficiency, nitrate reductase activity and seed yield in Brassica juncea. Photosynthet, 41: 281-284. (Journal)
Fatheiamirkhiz, K., Omidi, H., Heshmati, S. and Jafarzadeh, L. 2012. Evaluate the effect of accelerating on seed vigor and germination characteristics of medicinal plant Nigella sativa under salt stress. Iranian Journal of Field Crops Research, 10(2): 299-310. (Journal)(In Persian)
Ghana, S.G. and Schillinger, W.F. 2003. Seed priming winter wheat for germination, emergence, and yield. Crop Science, 43(6): 2135-2141. (Journal)
Ghasemi Jobshahr, E. and Khoramivafa, M. 2013. Effect of Pretreatment of Salicylic Acid on Germination and Seedling Properties Callendulla officinalis in Salt Stress Condition. Plant Production and Technology, 17(2): 57-70. (Journal) (In Persian)
Gu, Z., Chen, D., Han, Y., Chen, Z. and Gu, F. 2008. Optimization of carotenoids extraction from Rhodobacter sphaeroides, Learning With Technologies, 41: 1082–1088. (Journal)
Halmer, P. 2004. Methods to Improve Seed Performance in the Field. In: Benech-Arnold RL, Sanchez RA (Eds), Handbook of Seed physiology: Application to Agriculture, Pp: 65-125. (Book)
ISTA (International Seed Testing Association). 2008. International rules for seed testing. Seed Science and Technology. 24: 155-202. (Handbook)
Jamil, M., Rehman, S. and Rha, E.S. 2007. Salinity Effect on plant growth, PSII photochemistry and chlorophyll content in sugar beet (Beta vulgaris L.) and cabbage (Brassica oleracea capitata L.). Pakistan Journal of Botany, 39: 753-760. (Journal)
Kanawapee, N., Sanitchon, J., Lontom, W. and Threerakulpisut, P. 2012. Evaluation of salt tolerance at the seedling stage in rice genotypes by growth performance, ion accumulation, proline and chlorophyll content. Plant and Soil, 358: 235-249. (Journal)
Kaur, S., Gupta, A.K. and Kaur, N. 2002. Effect of osmo and hydro priming of chickpea seeds on seedling growth and carbohydrate metabolism under water deficit stress. Plant Growth Regulation, 37(1): 17-22. (Journal)
Kaur, S., Gupta, A.K. and Kaur, N. 2005. Seed priming increase crop yield possibly by modulating enzymes of sucrose metabolism in chickpea. Journal of Agronomy and Crop Science, 191(2): 81-87. (Journal)
Khan, W., Prithviraj, B., Smith, D.L., 2003. Photosynthetic responses  of cornand soybean to foliar application of salicylates. J. Plant Physiology, 160: 485-492. (Journal)
Kumari, N., Rai, P.K., Bara, B.M. and Singh, I. 2017. Effect of halo priming and hormonal priming on seed germination and  seedling vigour in  maize (Zea mays  L) seeds. Journal of Pharmacognosy and Phytochemistry, 6(4): 27-30. (Journal)
Mandegari, A., Pournamdari, M., Sharififar, F., Pournourmohammadi, Sh. Fardiar, R. and Sholi, S. 2012. Alkaloid and flavonoid rich fractions of fenugreek seeds (Trigonellafoenum-graecum L.) with antinociceptive and anti- inflammatory effects. Food and Chemical Toxicology, 50: 2503-2507. (Journal)
Mehrinfar, F., Nematzadeh, G., Pirdashti, H., Makarer, H. 2015. The Effect of Salinity on ionic Content, Plant Pigment and Soluble Sugar and Starch Halophyte Aeluropus Plant (Aeluropus  littoralis) New Findings in Agriculture.8(3): (Journal)
Mir Sadegi, S., Shekari, F., Fotovat, R. and Zangani, E. 2011. The effect of priming by salicylic acid on vigor and seedling growth of canola (Brassica napus) under water deficit condition. Journal of Plant Biology, 6: 55-70. (Journal)(In Persian)
Misra, N. and Dwivedi, U.N. 2004. Genotypic difference in salinity tolerance of green gram cultivars. Plant Science, 166: 1135-1142. (Journal)
Misra, N. and Saxena, P. 2009. Effect of salicylic acid on proline metabolism in lentilgrown under salinity stress. Plant Science, 599: 525–529.
Mittal, S., Kumari, N. and Sharma, V. 2012. Differential response of salt stress on Brassica juncea: Photosynthetic performance, pigment, proline, D1 and antioxidant enzymes, Plant Physiology and Biochemistry, 54: 17-26. (Journal)
Paparella, S., Araújo, S.S., Rossi, G., Wijayasinghe, M., Carbonera, D. and Balestrazzi, A. 2015. Seed priming: state of the art and new perspectives. Plant Cell Reports, 34 (8): 1281-1293. (Journal)
Parida, A.K., Das, A.B. and Mohanty, P., 2004. Defense potentials to NaCl in a mangrove, Bruguiera  parviflora: differential  changes  of  isoforms of some antioxidative enzymes. Journal of Plant Physiology, 161(5): 531-542. (Journal)
Roumani, A. and Ehteshami, S.M. 2014. Effect of different levels of salinity stress on seed germination and early growth of fenugreek (Trigonella foenum L.) seedling. Journal of Seed Research, 1(1): 33-45. (Journal)(In Persian).
Saadatyan, B., Ahmadvand, Gh. and Soleimani, F. 2012. Seed priming effect on Satureja hortensis characteristics of drought stress and salt stress. Journal of Science and Technology of Seed, 2(2): 44-33. (Journal)(In Persian)
Saeedi Goraghani, H.R., Ranjbar Fordoei, A., Soleimani Sardo, M. and Mahdavi, M.J. 2017. Effect of salinity and drought stresses on germination stage and growth of black cumin (Bunium Persicum Boiss). Iranian Journal of Field Crops Research, 15(1): 1-7. (Journal)(In Persian)
Sarahi Nobar, M., Niknam, M. and Moradi, B. 2010. Effect of salinity stress on protein content, pigments, sugars and phenolic compounds in tissue culture of several species of Iranian fenugreek. Sciences University of Tehran, 36(2): 59-53. (Journal)(In Persian)
Shakirova, F.M., Sakhabutdinova, A.R. Bezrukova, M.V. Fatkhutdinova, R.A. and Fatkhutdinova. D. R. 2003. Changes in the hormonal status of wheat seedling induced by salicylic acid and salinity. Journal of Plant Science, 164: 317-322. (Journal)
Shakirova, F.M. and D.R. Sahabutdinova. 2003. Changes in the hormonal status of wheat seedlings induced by salicylic acid and salinity.Plant Science, 164: 317-322. (Journal)
Shekari, F., Baljani, R., Saba, J., Afsahi, K. and Shekari, F. 2010. Effect of seed priming with salicylic acid on growth characteristics of borage (Borago officinalis) plants seedlings. Journal of New Agricultural Science, 6: 47-53. (Journal)(In Persian)
Soltani, A., Gholipoor, M. and Zeinali, E. 2006. Seed reserve utilization and seedling growth of wheat as affected by drought and salinity. Environmental and Experimental Botany, 55: 195-200. (Journal)
Yagmur, M. and Kaydan, D. 2008. Alleviation of osmotic stress of water and salt in germination and seedling growth of triticale with seed priming treatments. African Journal of Biotechnology, 7 (13): 2156-2162. (Journal)
Yousefian, M., Tatian, M., Tamartash, R. and Montazeri, F. 2012. Investigation of Salt Stress on Seed Germination of Trigonella foenum-graecum. National Conference on Environment and Plant Production. Islamic Azad University of Damghan. 15th and 16th of October, 2012. (Journal)(In Persian)
Zalai, G., Tari, I., Janda, T., Pestenácz, A. and Páldi, E. 2000. Effects of cold acclimation and salicylic acid on changes in ACC and MACC contents in maize during chilling. Biology of Plant, 43: 637-640. (Journal)