تاثیر پرایمینگ بر شاخص‌های جوانه‌زنی و فعالیت آنزیمی بذر توسکای ییلاقی (Alnus subcordata) تحت شرایط روشنایی و تاریکی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی علوم باغبانی، دانشکده علوم زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

2 استادیار علوم باغبانی، دانشکده علوم زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

چکیده

توسکای ییلاقی به­عنوان درخت زینتی بومی جنگل­های شمال ایران، جهت استفاده در فضای سبز شهری کاربرد فراوانی دارد. با این حال ازدیاد این گیاه و تولید نهال آن در ایران صورت نمی­گیرد. یکی از روش­های آسان تکثیر گیاهان استفاده از بذر می­باشد. برای بهبود و افزایش جوانه­زنی بذر می­توان از پرایمینگ استفاده نمود. این تحقیق به­منظور بررسی تأثیر پرایمینگ (بدون پرایمینگ (شاهد)، نیترات پتاسیم 02/0 و 04/0 درصد، اسید جیبرلیک 50 و 100 میلی­گرم در لیتر، آب) و فتوپریود (تاریکی و روشنایی) بر شاخص­های جوانه­زنی و فعالیت آنزیمی توسکای ییلاقی انجام گرفت. آزمایش به­صورت فاکتوریل در قالب طرح کاملا تصادفی به اجرا در آمد. بیش­ترین درصد جوانه­زنی (53 درصد) در بذرهای پرایم­شده با اسید جیبرلیک 100 میلی­گرم در لیتر و در تاریکی بود. اثر پرایمینگ بر فعالیت آنزیم کاتالاز در سطح احتمال یک درصد معنی­دار بود. بیش­ترین فعالیت این آنزیم در بذرهای پرایم­شده با اسید جیبرلیک 100 میلی­گرم در لیتر بود. حداکثر فعالیت آنزیم پراکسیداز در در تاریکی و در تیمار 100 میلی­گرم در لیتر اسید جیبرلیک بود. بیش­ترین طول ساقه در تاریکی و روشنایی به­ترتیب با 8/3 و 7/2 سانتی­متر و در اسید جیبرلیک 50 میلی­گرم در لیتر بود. حداکثر شاخص بنیه مربوط به تیمار با اسید جیبرلیک بود. با توجه به نتایج به­دست­آمده استفاده از تیمار جیبرلیک اسید و شرایط تاریکی برای بهبود مولفه های جوانه­زنی در بذر توسکا توصیه می­شود.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of priming on seed germination indices and enzyme activity of Alnus subcordata under light and dark conditions

نویسندگان [English]

  • Narges Hajian 1
  • Mahnaz Karimi 2
1 BSc. Student of Horticultural Sciences, Faculty of Crop Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
2 Assistant Professor, Department of Horticultural Sciences, Faculty of Crop Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
چکیده [English]

Alder trees as ornamental tree in northern forests of Iran are most used in urban green space. However, its propagation and seedling production is not done in Iran. One of the easy methods of plant propagation is the use of seed. Seed priming technique can be used to improve seed germination. This experiment was conducted in order to study the effects of priming (without priming, potassium nitrate (0.02 and 0.04%, gibberellic acid 50 and 100 mg L-1, water) and Photoperiod (dark and bright) on germination and enzymatic activity of Alnus subcordata. The experiment was carried out in factorial arrangement based on completely randomized design. According to the results, the highest percentage of germination (53%) was observed in seeds primed with 100 mg L-1 gibberellic acid in darkness. The effect of priming on the activity of catalase enzyme was significant at 1% probability level. The maximum peroxidase activity was in the dark and in the treatment of 100 mg L-1 of gibberellic acid.The most activity of this enzyme was observed in seeds primed with gibberellic acid 100 mg L-1. The maximum length of stems in dark and light treatments was 3.8 and 2.7 cm, respectively, at 50 mg L-1 gibberellic acid treatment. The maximum vigor index was related to gibberellic acid treatment. According to the results, the use of gibberellic acid treatment and dark conditions are recommended to improve the characteristics of germination in alder seed.

کلیدواژه‌ها [English]

  • Germination Percentage
  • Gibberellic acid
  • Light treatments
  • Potassium nitrate
  • Priming Technique
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