ارزیابی تأثیر اسموهیدروپرایمینگ بذر بر مشخصه‌های جوانه‌زنی و رشد گیاهچه پنبه تحت تنش خشکی

نوع مقاله : مقاله پژوهشی

نویسنده

استادیار بخش تحقیقات زراعی و باغی، مرکز تحقیقات کشاورزی و منابع طبیعی استان خراسان رضوی، سازمان تحقیقات، آموزش و ترویج کشاورزی، مشهد، ایران

چکیده

به­منظور بررسی اثر اسموهیدروپرایمینگ بذر بر جوانه­زنی و رشد گیاهچه پنبه در شرایط تنش خشکی، دو آزمایش در مراحل جوانه­زنی و گیاهچه­ای صورت گرفت. در آزمایش اول، بذور پنبه پیش­تیمارشده با استفاده از آب مقطر (هیدروپرایمینگ) و پلی­اتیلن­گلایکول 6000 در پتانسیل­های 2-،4-، 6- و 8- بار (اسموپرایمینگ) به­همراه بذور شاهد در معرض پنج سطح پتانسیل اسمزی (صفر، 2-، 4-، 6- و 8- بار) محیط کاشت بذور قرار گرفتند. این تحقیق به­صورت فاکتوریل در قالب طرح کاملاً تصادفی انجام شد. دومین آزمایش با استفاده از بذرهای شاهد (بدون پیش­تیمار) و پیش­تیمارشده با آب مقطر (هیدروپرایم) و نیز پلی­اتیلن­گلایکول در سطح 2-، 4-، و 6- بار در سه سطح تنش آبی: 30، 60 و90 درصد ظرفیت زراعی با استفاده از گلدان­های با حجم دو لیتر در محل ایستگاه تحقیقات کشاورزی و منابع طبیعی کاشمر انجام شد. نتایج آزمایش جوانه­زنی نشان داد که هیدروپرایمینگ بذر پنبه بیش­ترین درصد و سرعت جوانه­زنی و همچنین وزن خشک گیاهچه را در تمامی پتانسیل­های اسمزی (به­جز 4- بار) در مقایسه با شاهد ایجاد نمود. نتایج آزمایش گلدانی نشان داد تیمارهای اسمو و هیدروپرایمینگ بذر پنبه، سبب افزایش معنی­دار ارتفاع گیاهچه، سطح برگ، وزن خشک بخش هوایی و ریشه نسبت به شاهد شد. تنش خشکی در تیمارهای 30 و60 درصد ظرفیت زراعی موجب کاهش سطح برگ و ارتفاع گیاهچه شد.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of the effect of seed osmo-hydropriming on germination indices and plantlet growth of cotton under drought stress

نویسنده [English]

  • Hamid Reza Mehrabadi
Assistant Professor, Department of Agronomic and Horticultural Crops Research, Khorasan Razavi Agricultural and Natural Resources Research Center, AREEO, Mashad, Iran
چکیده [English]

In order to survey of the effect of osmohydropriming on germination and growth of cotton plantlet under drought stress, two experiments were carried out at emergence and plantlet stages. In the first experiment, cotton seeds were primed with distilled water (hydropriming) and Poly Eltylene Glychol 6000 (-2, -4, -6, -8 bar) (osmopriming) and controlled seeds were subjected to 5 levels of osmotic potential (0, -2, -4, -6, -8 bar). The experiment was conducted as a factorial experiment based on completely randomized design. The second experminent were conducted with control and pretretmented seeds (hydroprimed) and osmoperimed seeds using Ploy Ethylene Glycol at three osmotic levels -2, -4 and -6 bar under three levels of drought stress 30, 60 and 90 percentage of field capacity (FC) using two-liter pots at Agricultural and Natural Resource Research Station of Kashmar. The results of experiment test showed, hydropriming of cotton seed had the most growth percentage and rate as well as dry weight of seedlings in all treatments (except -4 bar) in comparison with control. The results of pots experiment indicated, osmo and hydropriming of cotton-seed increased growth (plantlet height), leaf area, dry weight of shoot and root significantly in compaison with control. Drought stress at 30 and 60 percent of (FC) treatments decreased leaf area and plantlet height.

کلیدواژه‌ها [English]

  • Gossypium hirsutum
  • Leaf area
  • Osmotic stress
  • Poly eltylene glychol
  • Root indices
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