تأثیر اسیدهای آلی و تنش شوری بر جوانه‌زنی بذر و خصوصیات فیزیولوژیک گیاهچه سرخارگل (Echinacea purpurea L.)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد علوم و تکنولوژی بذر، دانشکده علوم کشاورزی، دانشگاه شاهد، تهران، ایران

2 دانشیار، دانشکده علوم کشاورزی، دانشگاه شاهد، تهران، ایران

3 دانش‌آموخته زراعت، دانشکده علوم کشاورزی، دانشگاه شاهد، تهران، ایران

چکیده

به­منظور بررسی تأثیر هیومیک اسید و فولویک اسید بر خصوصیات فیزیولوژیک گیاهچه‌ سرخارگلتحت شوری، آزمایشی به­صورت فاکتوریل در قالب طرح پایه کاملاً تصادفی با سه تکرار در آزمایشگاه تکنولوژی بذر دانشگاه شاهد در سال 1395 اجرا شد. تیمارهای آزمایش شامل تنش شوری در چهار سطح (0، 4، 8 و 12 دسی­زیمنس بر متر)، هیومیک اسید در سه سطح (0، 200 و 400 میلی­گرم بر لیتر) و فولویک اسید در سه سطح (0، 200 و 400 میلی­گرم بر لیتر) بود. صفات مورد بررسی شامل درصد جوانه­زنی، محتوای نسبی آب، محتوای کلروفیل a، b، کل و محتوای کارتنوئید، پرولین و پروتئین بود. با توجه به نتایج تجزیه واریانس، اثر متقابل هیومیک اسید، فولویک اسید و تنش شوری بر صفات مورد بررسی به استثنای محتوای نسبی آب گیاهچه معنی­دار بود. کاربرد هیومیک اسید 200 میلی­گرم بر لیتر و فولویک اسید 400 میلی­گرم بر لیتر با میانگین 55/75 درصد بالاترین درصد جوانه­زنی را در تنش شوری ds/m 12 داشت. بر اساس نتایج تحقیق حاضر، هیومیک اسید بیش­ترین تأثیر را بر محتوای ترکیبات محلول مورد مطالعه داشت، به­گونه­ای که تا زمانی غلظت فولویک اسید 200 میلی­گرم بر لیتر یا صفر بود می­توان افزایش ترکیبات محلول را در سطوح هیومیک اسید مشاهده کرد. غلظت 400 میلی­گرم بر لیتر هیومیک اسید در عدم حضور فولویک اسید و تنش ds/m 12 دارای بیش­ترین محتوای کلروفیل کل بود. تنش شوری باعث کاهش جوانه­زنی بذر سرخارگل شد، کاربرد هیومیک اسید و فولویک اسید اثرات ناشی از تنش شوری را، هر چند اندک بهبود بخشیدند.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of organic acids and salt stress on germination of seed and physiological properties of (Echinacea Purpurea L.)

نویسندگان [English]

  • Fersheteh Azadbakht 1
  • Majid Amini Dehaghi 2
  • Khadijeh Ahmadi 3
  • Samira Alipour Gravand 1
1 M.Sc. Student of Seed Science and Technology, Faculty of Agricultural Sciences, University of Shahed, Tehran, Iran
2 Associate Professor, Faculty of Agricultural Sciences, University of Shahed, Tehran, Iran
3 M.Sc. Graduated of Agronomy, Faculty of Agricultural Sciences, University of Shahed, Tehran, Iran
چکیده [English]

In order to investigate the effect of humic and fulvic acid on physiological properties of seedling Echinacea purpurea under salinity, a factorial experiment was conducted in a completely randomized design with three replications at Seed Technology Laboratory Shahed University in year 2016. The treatments experiment included salinity at four levels (0, 4, 8 and 12 ds/m), humic acid in three levels (0, 200 and 400 mg/lit) and fulvic acid at three levels (0, 200 and 400 mg/lit). The studied traits included germination percentage, relative water content, chlorophyll a, b and total content, carotenoid, proline and protein content. According to the results of analysis of variance, interaction effect of humic acid, fulvic acid and salinity had a significant effect on traits except the relative water content. The highest germination percentage was obtained by application of humic acid 200 mg/lit and fulvic acid 400 mg/lit with an average of 75.55% in salinity. According to the results of this study, humic acid has the most effect on the of soluble compounds content, as long as the fulvic acid concentration is 200 mg/lit or zero, it is possible to see the increase of soluble compounds at the levels of humic acid. The concentration of 400 mg/lit humic acid in the absence of fulvic acid and lack of salt stress had the highest chlorophyll total content. Salinity stress reduced seed germination of Sarkhargol, Application of Humic Acid and fulvic Acid Effects Caused by Salt Stress, though slightly improved.

کلیدواژه‌ها [English]

  • Chlorophyll
  • Echinacea purpurea
  • Fulvic acid
  • Humic acid
  • Proline
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