تاثیر نیتریک اکسید بر جوانه‌زنی و فعالیت برخی آنزیم‌های آنتی‌اکسیدانی کنجد (Sesamun indicum) تحت تنش شوری

نوع مقاله : مقاله پژوهشی


1 دانشجوی دکتری

2 عضو هیئت علمی دانشگاه صنعتی شاهرود


این آزمایش با هدف بررسی تأثیر نیتریک اکسید از منبع سدیم نیتروپروساید بر ویژگی­های جوانه­زنی و فعالیت برخی آنزیم‌های آنتی اکسیدانی کنجد تحت تنش شوری به‌صورت فاکتوریل بر پایه طرح کاملاً تصادفی در سه تکرار در آزمایشگاه مرکز تحقیقات بذر کاشمر اجرا شد. تیمارها شامل پیش تیمار بذر با سدیم نیتروپروساید در شش سطح (بدون پیشتیمار، آب مقطر، 50، 100، 150 و 200 میکرومولار) و تنش شوری در سه سطح (صفر، 50 و 100 میلی‌مولار در لیتر) بود. نتایج نشان داد که تنش شوری موجب کاهش شاخص­های جوانه­زنی و افزایش فعالیت آنزیم های آنتی اکسیدانی کنجد شد. پیش تیمار بذر با سدیم نیتروپروساید موجب افزایش سرعت جوانه­زنی، طول و وزن ریشه چه و ساقه چه در شرایط تنش شد و عملکرد بذر را بهبود بخشید. همچنین فعالیت آنزیم های سوپراکسیددیسموتاز، گلوتاتیون ردوکتاز و آسکوربات پراکسیداز نیز تحت تیمارهای نیتریک اکسید افزایش یافت در حالی که فعالیت آنزیم کاتالاز و محتوای مالون دی آلدئید به عنوان شاخص تخریب غشاء کاهش یافت. به طور کلی غلظت مناسب و کارا برای کاهش اثرات شوری در گیاهچه کنجد در این آزمایش غلظت 150 میکرومولار سدیم نیتروپروسایدبود، همچنین به نظر می رسد غلظت‌های بالاتر از 150 میکرومولار سدیم نیتروپروساید، اثرات سمیت نیتریک اکسید را بر سلول القا کند.


عنوان مقاله [English]

The effect of nitric oxide on seed germination and activities of some antioxidant enzymes in sesame under salt stress

نویسندگان [English]

  • Alireza Fathi 1
  • Mehdi Baradaran 2
  • MohammadReza Amerian 2
چکیده [English]

The present experiment was aimed to investigate the effect of nitric oxide derived from sodium nitroprusside on sesame germination and some antioxidant enzymes activity under salinity stress in factorial manner and completely randomized design with three replications in seed research laboratory of Research Station in Eastern Iran (Kashmar). Treatments included seed pretreatment with sodium nitroprusside in six levels (zero, distilled water, 50, 100, 150 and 200 mM) and salinity stress at three levels (0, 50 and 100 mM l). The results showed that salt stress decreased germination rate, shoot and root length and dry weight and also were increase the activity of antioxidant enzymes superoxide dismutase, ascorbate peroxidase and glutathione reductase in sesame. Pretreatment seeds with SNP were increased seed germination, root and shoot length and weight. The enzyme activity of superoxide dismutase, glutathione reductase and ascorbate peroxidase treated with nitric oxide were increased, while catalase activity and the content of malondialdehyde as the index fell membrane degradation were decreased. In general, suitable and efficient concentration to reduce the effects of salinity on plant seeds in this experiment was 150 mM, because in this concentration was found the highest germination rate and dry weight of shoot and root. It also seems to concentrations above 150 mM sodium nitroprusside, toxic effects on the cells induce nitric oxide.

کلیدواژه‌ها [English]

  • Ascorbate peroxidase
  • Germination
  • Sodium nitroprusside
  • Superoxide dismutase
Abnoos, M., 2001. The physiological study of drought stress effects on germination and seedling stage of lentil cultivars. Plant physiology MS. Thesis, Ferdowsi University of Mashhad. (In Persian)
Akpan-Iwo, G., A. A. Idowu and S. M. Misari. 2007. Collection and evaluation of sesame (Sesamum spp.) germplasm in Nigeria. PGR Newsletter. 142:59-62. (Journal)
Bazrafshan A. H., and Ehsanzadeh P. 2016. Evidence for differential lipid peroxidation and antioxidant enzyme activities in Sesamum indicum L. genotypes under NaCl salinity. Journal of Agriculture Science and Technology. 18: 207-222. (In Persian) (Journal)
Beligni M, Lamattina L. 2000. Nitric oxide stimulates seed germination and de-etiolation, and inhibits hypocotyl elongation, three light-inducible responses in plants. Planta. 210:215-221. (Journal)
Beligni, M.V. and Lamitina, L. 1999. Nitric oxide protects against cellular damage produced by methyle viologen herbicides in potato plants. Nitric oxide. Biology and Chemistry. 3: 199-208. (Journal)
Bor, M., Ozdemir, F. and Tutkan I. 2002. The effect of salt stress on lipid peroxidation and antioxidants in leaves of sugar beet Beta vulgaris L. and wild beet (Beta maritima L.). Plant Science. 164: 77-84. (Journal)
Chen M, Shen W B, Ruan H H, Xu L L. 2004. Effects of nitric oxide on root growth and its oxidative damage in wheat seedling under salt stress. Journal of Plant Physiology and Molecular Biology. 30(5): 569-576 (in Chinese with English summary) (Journal)
Dakhil, B.B., Denden, M., 2010. Salt stress induced changes in germination, sugars, starch and enzyme of carbohydrate metabolism in Abelmoschus esculentus L. (Moench.) seeds. African Journal of Agricultural Research. 5(12): 1412-1418. (Journal)
Etesami, M., Galeshi. S. 2008. Evaluation reaction of ten genotype of barley in salinity on germination and seedling growth (Hordeum vulgar L.). Journal of Agricultural Science and Nature Resource. 15(5), 39-46. (In Persian) (Journal)
Fazeli, s., Nezami, A., Parsa, M., and Kafi, M. 2014. Evaluate germination and seedling growth of sesame ecotypes in saline conditions. Journal of Environmental Stresses in Crop Sciences. 7 (2): 217-232. (In Persian) (Journal)
Fan HF, Guo SR, Li J, Du CX, Huang BJ. 2007. Effects of exigenous nitric oxide on Cucumis sativus seedlings growth and osmotic adjustment substances contents under NaCl stress. Chines Journal of Ecology. 26:2045-2050. (Journal)
Farooq, M., Basra, S.M.A., Wahid, A. and Rehman, H. 2009. Exogenously applied nitric oxide enhances the drought tolerance in fine grain aromatic rice. Journal of Agronomy and Crop Science. 195: 254-261. (Journal)
Foyer, C. H. and Halliwell, B. 1976. The presence of glutathione and glutathione reductase in chloroplasts: A proposed role in ascorbic acid metabolism. Planta.133: 21-25. (Journal)
Giba Z, Grubisic D, Todorovic S, Sajc L, Stojakovic D, Konjevic R. 1998. Effect of nitric oxide releasing compounds on phytochromecontrolled germination of empress tree seeds. Plant Growth Regulators 26: 175-181. (Journal)
Heath, R.L. and Packer, L. 1968. Photoperoxidation in isolated chloroplasts. Archives of Biochemistry and Biophysics. 125: 189-198. (Journal)
Kenneth, K.T. 1990. Plant responses to saline and sodic condition. PP: 113-138. In: Agricultural salinity assessments and management, scientific publishers.
Koca, H., Bor, M., Özdemir, F. and Türkan, I. 2007. The Effect of salt stress on lipid peroxidation, antioxidative enzymes and proline content of sesame cultivars. Environmental and Experimental Botany. 60: 344-351. (Journal)
Kopyra, M. and E.A. Gwozdz. 2004. The role of nitric oxide in plant growth regulation and responses to abiotic stresses. Acta Physiologiae Plantarum. 26: 459-472. (Journal)
Langham, D. R., G. Smith, T. Wiemers, and J. Riney. 2006. Southwest sesame grower’s pamphlet. Sesaco Corporation, San Antonio, Texas.
Leshem, Y.Y. and Pinchasov, Y. 2000. Non-invasive photoacoustic spectroscopic determination of relative endogenous nitric oxide and ethylene content stoichiometry during the ripening of strawberries Fragaria ananasa (Duch.) and avocados persea americana (Mill.). Journal of Experimental Botany. 51: 1471-1473. (Journal)
Liu S., Dong Y., Xu L., Kong J. 2007. Effects of foliar applications of nitric oxide and salicylic acid on salt-induced changes in photosynthesis and antioxidative metabolism of cotton seedlings. Plant Growth Regulator. 73:67-78. (Journal)
López-Carrión, A.I., Castellano, R., Rosales, M.A., Ruiz, J.M., Romero, L.: 2008. Role of nitric oxide under saline stress: implications on proline metabolism. Biologia Plantarum 52: 587-591.
Maguire, J. D. 1962. Speed of germination-aid in selection and evaluation for seedling emergence and vigor. Crop Science. 2:176-177. (Journal)
Mahmood, S., Iram, S., Athar, H., 2003. Intraspecific variability in sesame (Sesamum indicum L.) for various quantative and qualitative attributes under differential salt regimes. Journal of Research Science 14(2), 177-186.
Meloni, D.A., Oliva, M.A., Martinez, C.A, and Cambraia, J. 2003. Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. Environmental and Experimental Botany. 49: 69–76. (Journal)
Mittler, R., Vanderauwera, S., Gollery, M. and Breusegem F.V. 2004. Reactive oxygen gene network of plants. Trends in Plant Science. 9: 490-498. (Journal)
Nakano, Y. and Asada, K. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiology.22:867-880. (Journal)
Sairam, R.K., K. Veerabhadra Rao and G.C. Srivastava. 2002. Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Science. 163: 1037-1046. (Journal)
Sairam, R.K. and G.C. Srivastava. 2001. Water stress tolerance of wheat Triticum aestivum L.: Variation in hydrogen peroxide accumulation and antioxidant activiy in tolerant and susceptible genotype. Journal of Agronomy and Crop Science. 186: 63-70. (Journal)
Sheokand, S., Kumari, A., Sawhney, V.: 2008. Effect of nitric oxide and putrescine on antioxidative responses under NaCl stress in chickpea plants. Physiology and Molecular Biology of Plants. 14: 355-362. (Journal)
Shi Q, Ding F, Wang X, Wei M. 2007. Exogenous nitric oxide protect cucumber roots against oxidative stress induced by salt stress. Plant Physiology and Biochemistry 45:542-550.
Tester, M. and Davenport, R. 2003. Na+ tolerance and Na+ transport in higher plants. Annals of Botany. 91: 503-527. (Journal)
Torabian S., Zahedi M., and Khoshgoftarmanesh A. 2016. Effect of foliar spray of zinc oxide on some antioxidant enzymes activity of sunflower under salt stress. Journal of Agricultural Science and Technology. 18: 1013-1025. (Journal)
Uchida, A., A.T. Jagendorf, T. Hibino, T. Takabe and T. Takabe. 2002. Effects of hydrogen peroxide and nitric oxide on both salt and heat stress tolerance in rice. Plant Science 163: 515-523.
Vaishnav A., S. Jain, A. Kasotia, S. Kumari, R, Kumar Gaur, D. Kumar Choudhary. 2013. Effect of nitric oxide signaling in bacterial‑treated soybean plant under salt stress. Archives of Microbiology. 195:571–577. (Journal)
Valdiani, A.R., Hassanzadeh, A., Tajbakhsh, M., 2005. Study on the effects of salt stress in germination and embryo growth stages of the four prolific and new cultivars of winter rapeseed (Brassica napus L.). Pajouhesh and Sazandegi. 66, 23-32. (In Persian) (Journal)
Wieczorek, J.F., Milczarek, G., Arasimovicz, M. and Ciszewski, A. 2006. Do nitric oxide donors mimic endogenous No- related response in plants. Planta 224: 1363-1372. (Journal)
Xie, Y.J., Lin, T.F., Han, Y., Liu, K., Zheng, Q., Huang, L., Yuan, X., He, Z., Hu, B., Fang, L., Shen, Z., Yang, Q., Shen, W.: 2008. Carbon monoxide enhances salt tolerance by nitric oxide-mediated maintenance of ion homeostasis and up-regulation of antioxidant defense in wheat seedling roots. Plant Cell and Environment. 31: 1864-1881. (Journal)
Zeinali, E., Soltani, A., Galeshi, S., 2001. Response of germination components to salinity stress in oilseed rape (Brassica napus L.). Journal of Agriculture Science and Natural Resource. 33(1), 137-145. (In Persian) (Journal)