اثر چینه‌سرمایی و تنظیم‌کننده‌های رشد گیاهی بر جوانه‌زنی بذر شقایق ایرانی (Glaucium corniculatum)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 بخش علوم باغبانی دانشگاه شیراز

2 استادیار بخش علوم باغبانی دانشگاه شیراز

چکیده

برای حل مشکل جوانه­زنی بذرهای شقایق ایرانی، سه آزمایش با طرح کاملاً تصادفی اجرا شد. آزمایش اول شامل چینه­سرمایی 5 درجه سلسیوس (10، 20، 30، 40، 50، 60، 70، 80، 90 و 100 روز) بود. آزمایش دوم در بردارنده تیمارهای اسید جیبرلیک (0، 100، 150، 200، 250، 300، 350، 400، 450 و 500 میلی­گرم در لیتر)، بنزیل­آدنین (0 ، 25، 50، 100 و 200) و استریگولاکتون (0، 01/0، 1/0 و 1 میلی­گرم در لیتر) بود. آزمایش سوم ترکیبی از بهترین تیمارهای آزمایش اول و دوم شامل 100 میلی­گرم در لیتر اسید جیبرلیک + 50 میلی­گرم در لیتر بنزیل­آدنین، 400 میلی­گرم در لیتر اسید­جیبرلیک + 200 میلی­گرم در لیتر بنزیل­آدنین، 100 میلی­گرم در لیتر اسید جیبرلیک + چینه­سرمایی 60 روزه و 100 میلی­گرم در لیتر بنزیل­آدنین + چینه­سرمایی 60 روزه بود. نتایج آزمایش اول با اختلاف معنی­دار نسبت به شاهد نشان داد بیشترین میانگین روزانه، سرعت و درصد جوانه­زنی به ترتیب در تیمارهای 30، 60 و 70 روز چینه­سرمایی با مقادیر 86/1 و 4/3 و 81 درصد است. نتایج آزمایش دوم نشان داد اسید جیبرلیک تأثیر معنی­داری بر جوانه­زنی بذر در مقایسه با تیمارهای شاهد، بنزیل­آدنین و استریگولاکتون دارد. حداکثر جوانه­زنی بذرها با کاربرد 100 میلی­گرم در لیتر اسیدجیبرلیک بدست آمد. آزمایش سوم نشان داد ترکیب تیمارهای چینه­سرمایی 60 روزبا 100 میلی­گرم در لیتر اسیدجیبرلیک در مقایسه با کاربرد تنهای 100 میلی­گرم در لیتر اسیدجیبرلیک شاخصهای درصد (25/81%)، سرعت (23/5) و میانگین جوانه­زنی روزانه (35/1) را ده برابر افزایش داده است، که می­توان تأثیر اصلی آن را به 60 روز سرما نسبت داد.

کلیدواژه‌ها

عنوان مقاله [English]

Effects of cold stratification and plant growth regulators on seed germination of Iranian horned poppy (Glaucium corniculatum)

نویسندگان [English]

  • Kazem Bashiri 1
  • Abolfazl Jowkar 2
  • Akbar Karami 1
چکیده [English]

Toward overcoming the seed germination problem of Iranian poppy, three experiments with a completely randomized design were conducted. The first experiment consisted of 5 ◦C cold stratification (10, 20, 30, 40, 50, 60, 70, 80, 90 & 100 days). The second experiment was composed of gibberellic acid (100, 150, 200, 250, 300, 350, 400, 450 & 500 mg/L), benzyl adenine (0, 25, 50, 100 & 200 mg/L) and strigolactones (0, 0.01, 0.1 & 1 mg/L). The third experiment was combined of the first and second experiments’ best treatments including 50 mg/L benzyl adenine + 100 mg/L gibberellic acid, 200 mg/L benzyl adenine + 400 mg/L gibberellic acid, 100 mg/L benzyl adenine + cold stratification at 5 ◦C for 60 days and 100 mg/L gibberellic acid + cold stratification at 5 ◦C for 60 days. Results of the first experiment showed that highest mean daily, rate and germination percentage was observed in 30, 60 and 70 days of cold stratification respectively, with amounts of 1.86, 3.4 and 81 percent. Results of the second experiment revealed that gibberellic acid had significant impacts on seed germination, in comparison with control, benzyl adenine and strigolactone. The maximum seed germination was observed using 100 mg/L of gibberellic acid. The third experiment demonstrated that combination of 100 mg/L gibberellic acid with cold stratification at 5 ◦C for 60 days increased germination percentage (81.25%), germination rate (5.23) and mean daily germination (1.35) for ten times, though its main effect could be attributed to 60 days of cold

کلیدواژه‌ها [English]

  • Iranian horned poppy
  • Plant growth regulators
  • Stratification
  • Strigolactone

مراجع

Allen, P. S., Benech-Arnold, R. L., Batlla, D. and Bradford, K. J. 2007. Modeling of seed dormancy. In: Bradford, K. J. and Nonogaki, H. (Eds.). Seed development, dormancy and germination. Blackwell Publishing, Oxford, UK. pp: 72-112. (Journal)
Bewley, D., Bradford, K. J., Hilhorst, H. W. M. and Nonogaki, H. 2013. Seeds – Physiology of development, germination and dormancy (3rd ed.). Springer. (Book)
Bicalho, E. M., Pinto-Marijuan, M., Morales, M., Muller, M., Munne-Bosch, S. and Garcia, Q. S. 2015. Control of macaw palm seed germination by the gibberellin/abscisic acid balance. Plant Biology, 17: 990-996. (Book)
Bourgoin, A. and Simpson, J. D. 2004. Soaking, moist-chilling, and temperature effects on germination of Acer pensylvanicum seeds. Canadian Journal of Forest Research, 34 (10): 2181-2185. (Journal)
Bouwmeester, H. J., Matusova, R., Zhongkui, S. and Beale, M. H. 2003. Secondary metabolite signalling in host-parasitic plant interactions. Current Opinion in Plant Biology, 6: 358-364. (Book)
Cheng, X., Ruyter-Spira, C. and Bouwmeester, H. 2013. The interaction between strigolactones and other plant hormones in the regulation of plant development. Frontiers in Plant Science, 4 (199): 1-16. (Journal)
Chiwocha, S. D. S., Dixon, K. W., Flematti, G. R., Ghisalberti, E. L., Merritt, D. J., Nelson, D. C.,  Riseborough, J. M., Smith, S. M. and Stevens, J. C. 2009. Karrikins: A new family of plant growth regulators in smoke. Plant Science, 177: 252-256. (Journal)
Coons, J., Coutant, N., Lawrence, B., Finn, D. and Finn, S. 2014. An effective system to produce smoke solutions from dried plant tissue for seed germination studies. Applications in Plant Science, 2: 1-3. (Book)
Dahkaei, M. N. P. 2009. Effect of gibberellic acid, temperature and cold moist stratification on seed germination of Danae racemosa. Acta Horticulturae, 813: 445-452. (Journal)
Del Pozo, J. C., Lopez-Matas, M. A., Ramirez-Parra, E. and Gutierrez, C. 2005. Hormonal control of the plant cell cycle. Physiologia Plantarum, 123: 173-183. (Book)
Deno, N. C. 1993. Seed germination theory and practice (2nd ed.). Pennsylvania state university. (Book)
Dhupper, R. 2013. Effect of gibberellic acid on seed germination and seedling growth behaviour in three desert tree species. The Journal of Biological Chemistry research, 30 (1): 227-232. (Journal)
Doncheva, T., Kostova, N., Yordanova, G., Saadi, H., Akrib, F., Dimitrov, D. and Philipov, S. 2014. Comparison of alkaloid profile from Glaucium corniculatum (Papaveraceae) of Algerian and Bulgarian origin. Biochemical Systematics and Ecology, 56: 278-280. (Book)
El-Maarouf-Bouteau, H. and Bailly, C. 2008. Oxidative signaling in seed germination and dormancy. Plant Signaling and Behavior, 3: 175-182. (Journal)
Foley, M. E. 2001. Seed dormancy: an update on terminology, physiological, genetics, and quantitative trait loci regulating germinability. Weed Science, 49: 305-317. (Book)
Ghayyad, M., Kurbysa, M. and Napolsy, G. 2010. Effect of endocarp removal, gibberellin, stratification and sulfuric acid on germination of mahaleb (Prunus mahaleb L.) seeds. American-Eurasian Journal of Agricultural and Environmental Science, 9: 163-168. (Journal)
Ghebrehiwot, H. M., Kulkarini, M. G., Szalai, G., Soòs, V., Balázs, E. and Van staden, J. 2012. Karrikinolide residues in grassland soils following fire: Implications on germination activity. South African Journal of Botany, 88: 419-424. (Journal)
Gomez-Roldan, V., Fermas, S., Brewer, P. B., Puech-Pagès, V. and Dun, E. A. 2008. Strigolactone inhibition of shoot branching. Nature, 455: 189-194. (Book)
Gorian, F., Pasquini, S. and Daws, M. I. 2007. Seed size and chilling affect germination of Larix decidua Mill. Seeds. Seed Science and Technology, 35 (2): 508-513. (Journal)
Hartmenn, H. T., Kester, D. E., Devies, F. T. and Geneve, R. L. 2002. Plant propagation: Principles and practices (7th ed.). Prentice Hall. (Book)
Hassan, F. A. and Fetouh, M. I. 2014. Seed germination criteria and seedling characteristics of Magnolia grandiflora L. trees after cold stratification treatments. International Journal of Current Microbiology and Applied Sciences, 3 (3): 235-241. (Journal)
Huang, Z., Dong, M. and Gutterman, Y. 2004. Factors influencing seed dormancy and germination in sand, and seedling survival under desiccation, of Psammochloa villosa (Poaceae), inhabiting the moving sand dunes of Ordos, China. Plant Soil, 259: 231-241. (Book)
Kintsurashvili, L. G., Vachnadze, V. Y. 2000. Alkaloids of Glaucium corniculatum and G. flavum growing in Georgia. Chemistry of Natural Compounds, 36 (2): 225-226. (Journal)
Maguire, J. D. 1962. Speed of germination in selection and evaluation for seedling vigor. Crop Science, 2: 176-177. (Book)
Miransaria, M. and Smith, D. L. 2014. Plant hormones and seed germination. Environmental and Experimental Botany, 99: 110-121. (Book)
Nikolic, R., Mitic, N., Miletic, R. and Neskovic, M., 2006. Effects of cytokinins on in vitro seed germination and early seedling morphogenesis in Lotus corniculatus L. Journal of Plant Growth Regulation, 25: 187-194. (Journal)
Outcall, K.W. 1991. Aerated stratification improves germination of Ocala sand pine seed. Tree Planters Notes, 42 (1): 22-26. (Book)
Parvin, P., Khezri, M., Tavasolian, I. and Hosseini, H. 2015. The Effect of gibberellic acid and chilling stratification on seed germination of eastern black walnut (Juglans nigra L.) Journal of Nuts, 6 (1): 67-76. (Journal)
Penfield, S., Josse, E. M., Kannangara, R., Gilday, A. D., Halliday, K. J. and Graham, I. A. 2005. Cold and light control seed germination through the bHLH transcription factor spatula. Current Biology, 15: 1998-2006. (Book)
Riefler, M., Novak, O., Strnad, M. and Schmulling, T. 2006. Arabidopsis cytokinin receptor mutants reveal functions in shoot growth, leaf senescence, seed size, germination, root development, and cytokinin metabolism. Plant Cell, 18: 40-54. (Book)
Rietveld, P. L., Wilkinson, C., Franssen, H. M., Balk, P. A., van der Plas, L. H. W., Weisbeek, P. J and Douwe De Boer, A. 2000. Low temperature sensing in tulip (Tulipa gesneriana L.) is mediated through an increased response to auxin. Journal of Experimental Botany, 51 (344): 587-94. (Journal)
Stirk, W. A., Gold J. D., Novak, O., Strnad, M. and van Staden, J. 2005. Changes in endogenous cytokinins during germination and seedling establishment of Tagetes minuta L. Plant Growth Regulation, 47 (1): 1-7. (Book)
Tompsett, P. B. and Pritchard, H. W. 1998. The effect of chilling and moisture status on the germination, desiccation tolerance and longevity of Aesculus hippocastanum L. seed. Annals of Botany, 82: 249-261. (Book)
Wang, Y., Li, L., Ye, T., Zhao, S., Liu, Z., Feng, Y. and Wu, Y. 2011. Cytokinin antagonizes ABA suppression to seed germination of Arabidopsis by down regulating ABI5 expression. Plant Journal, 68: 249-261. (Journal)
Wilson, J. C., Altland, J. E., Sibley, J. L., Tilt, K. M. and Foshee, W. G. 2004. Effects of chilling and heat on growth of Ginkgo biloba L. Journal of Arboriculture, 30(1): 45-51. (Journal)
Wu, L., Hallgren, S. W., Ferris, D. M. and Conway, K. E. 2001. Effects of moist chilling and solid matrix priming on germination of loblolly pine (Pinus taeda L.) seeds. New Forests, 21: 1-16. (Book)
علوم و تحقیقات بذر ایران
دوره 4، شماره 3
آذر 1396
صفحه 53-61

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