اثر تنش مس، نیکل و کبالت بر خصوصیات جوانه‌زنی غده اویارسلام ارغوانی در شرایط آزمایشگاه

نوع مقاله: مقاله پژوهشی

نویسندگان

دانشگاه جیرفت

چکیده

به­منظور بررسی میزان مقاومت گیاه اویارسلام به تنش فلزات سنگین مس، نیکل و کبالت در مرحله جوانه­زنی، آزمایشی به­صورت فاکتویل در قالب طرح کاملا تصادفی با سه تکرار در آزمایشگاه زراعت و باغبانی دانشگاه جیرفت انجام شد. فاکتور اول شامل سه فلز مس، نیکل، کبالت و فاکتور دوم شامل سطوح غلظت فلز (0، 200، 400، 600، 800، 1000 میلی‌گرم بر لیتر) بود. نتایج نشان داد که صفات درصد، سرعت و متوسط زمان جوانه‌زنی به­طور معنی‌داری (p<0.01) تحت تاثیر فلزات سنگین و سطوح تنش و فقط درصد جوانه‌زنی تحت تاثیر اثر متقابل آن­ها قرار گرفتند. بررسی اثر سطوح تنش نشان داد تیمارهای 200، 400، 600، 800 و 1000 میلی‌گرم بر لیتر، میزان سرعت جوانه‌زنی غده‌ها را نسبت به شاهد به‌ترتیب 8/17%، 07/26%، 7/35%، 4/46% و 1/57% کاهش داد. تیمارهای مذکور درصد جوانه‌زنی غده‌ها را نیز به‌ترتیب 2/9%، 9/14%، 1/24%، 7/36% و 9/45% نسبت به شاهد کاهش داد. کم­ترین درصد (5/70) و سرعت جوانه‌زنی (7/1) در اثر تنش فلز نیکل اتفاق افتاد. نتایج تجزیه رگرسیون نشان داد تغییرات سرعت جوانه‌زنی تحت تاثیر سطوح تنش مس، نیکل و کبالت تابع یک منحنی درجه دوم با ضرایب تباین بالای 90% بود که در سطح 01/0 آماری معنی‌دار گردید. به­طورکلی نتایج حاصل از این آزمایش نشان داد اویارسلام گیاهی مقاوم به تنش فلزات سنگین است که حتی تا غلظت 1000 میلی‌گرم بر لیتر نیز قابلیت جوانه‌زنی و رشد دارد، همچنین قابلیت تحمل اویارسلام به فلز سنگین مس نسبت به فلزات کبالت و نیکل بیش­تر بود Ni <Co <Cu.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of copper, nickel and cobalt stress on tuber germination traits of Cyperus rotundus under in vitro conditions

نویسندگان [English]

  • Shahin Jahan Nejati
  • Mehrangiz Jokar
  • Javad Taei Semiromi
چکیده [English]

In order to evaluate cyperus rotundus resistance to heavy metals stress, copper, nickel and cobalt, in the germination stage, the experiment was conducted in factorial based on completely randomized design with three replications in agronomy and horticulture laboratory of Jiroft University. The first factor consists of three metal copper, nickel, cobalt and the second factor levels consists of metal concentrations (0, 200, 400, 600, 800, 1000 ppm), respectively. The results showed that germination percentage, rate and mean germination time were affected significantly (p <0.01) affected by heavy metals and levels of stress and only, germination percentage were affected by their interactions. The effect of stress levels showed that treatments of 200, 400, 600, 800 and 1000 ppm, compared to control the tubers germination rate decreased tubers 17.8%, 26.07%, 35.7%, 46.4% and 57.1%, respectively. These treatments decreased tubers germination percentage respectively 9.2%, 14.9%, 24.1%, 36.7% and 45.9% compared to the control. The lowest germination percentage (70.5) and rate (1.7) happened in nickel stress. The Regression analysis results showed that germination rate changes affect the stress levels of copper, nickel and cobalt fit on quadratic function with R- square (R2) above 90%, which was statistically significant at 0.01. In general, the results of this experiment showed cyperus rotundus is tolerance to heavy metals stress so that, even able to germination and growth at concentrations up to 1000 mg/L, also tolerance Capability cyperus rotundus to copper heavy metal was higher than cobalt and nickel i.e. Cu >Co >Ni.

کلیدواژه‌ها [English]

  • Cyperus rotundus
  • germination percentage
  • Germination rate
  • Heavy metals
  • Tolerance
  • Tuber
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