تأثیر پیش تیمار بذر با باکتری های محرک رشد بر شاخص های جوانه زنی بذر کتان روغنی (Linum usitatisimum L) تحت تنش شوری

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی گیاهان زراعی دانشکده کشاورزی دانشگاه شاهد تهران

2 دانشجوی کارشناسی ارشد زراعت دانشکده کشاورزی دانشگاه شاهد تهران

3 دانشجوی کارشناسی ارشد آگرواکولوژی دانشکده کشاورزی دانشگاه شاهد تهران

4 دانشجوی کارشناسی ارشد تکنولوژی بذر دانشکده کشاورزی دانشگاه شاهد تهران

چکیده

این تحقیق به­منظور بررسی پیش­تیمار بذر با باکتری­های محرک رشد بر مؤلفه‌های جوانه­زنی بذر کتان روغنی در شرایط تنش شوری به­صورت فاکتوریل در قالب طرح کاملاً تصادفی در­ سه تکرار در آزمایشگاه علوم وتکنولوژی بذر دانشگاه شاهد در سال 1393 انجام گرفت. تیمارهای آزمایش شامل پیش­تیمار با باکتری­های محرک رشد در پنج سطح، عدم پیش تیمار به­عنوان شاهد، ازتوباکتر کروکروم (سویه Azeto-5)، سدوموناس فلورسنس (سویه P-169)، سدوموناس پوتیدا (سویه P-168) و کود زیستی فسفر بارور2 و تنش شوری در پنج سطح، صفر به عنوان شاهد، 5/2، 5، 5/7 و 10­ دسی­زیمنس بر متر بودند. نتایج نشان داد بیش­ترین میانگین آلومتری (31/1) مربوط به شوری 10 دسی‌زیمنس بود. بیش­ترین میانگین شاخص طولی بنیه گیاهچه (83/1375)، طول ریشه­چه (83/6 سانتی­متر) و طول گیاهچه (14 سانتی­متر) در برهمکنش پرایمینگ با فسفر بارور2 در شوری 5/2 دسی­زیمنس بر متر به دست آمد. بالاترین میانگین متوسط زمان لازم برای جوانه­زنی (51/2)، شاخص وزنی بنیه گیاهچه (87/1) و وزن خشک گیاهچه (019/0 گرم) مربوط به پرایمینگ با سدوموناس فلورسنس در شوری 10 دسی­زیمنس بر متر بود. عدم پرایمینگ (شاهد) در سطح شوری صفر دسی­زیمنس بر متر (شاهد) نیز دارای بیش­ترین میانگین سرعت جوانه­زنی (33/15 بذر در روز) بود. به­طور کلی می­توان نتیجه گرفت که استفاده از کودهای زیستی فسفر بارور 2 و سودوموناس فلورسنس می­تواند در تعدیل تنش شوری بر مؤلفه‌های جوانه­زنی بذر به­خصوص افزایش رشد گیاهچه و بنیه بذر کتان روغنی مؤثر ­باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of seed priming with plant growth promoting rhizobacteria (PGPR) on germination components of flaxseed (Linum usitatisimum L.) under salinity stress

نویسندگان [English]

  • Mehdi Aghighi Shahverdi 1
  • Hojjat Attaei Somagh 2
  • Behnam Mamivand 3
  • Sharifeh Habibipour 1
  • Milad Hemati 4
چکیده [English]

This study aimed to evaluate the effect of seed priming with plant growth promoting rhizobacteria (PGPR) on germination components of flaxseed under salinity conditions a factorial experiment was conducted a completely randomized design (CRD) with three replications in the Seed Science and Technology laboratory of Shahed University in 2014. Experimental factors consisted of five levels salinity stress, zero as a control, 2.5, 5, 7.5 and 10 dS.m-1 and pre-treatment with PGPR in five levels, no priming as a control, Azotobacter (strain Azeto-5), Pseudomonas fluorescent (strain P-169), Pseudomonas putida (strain P-168) and biological phosphorus fertilized 2. Results showed that the high mean allometric (1.31) corresponds to a salinity of 10 dS.m-1. The interaction of priming with biological phosphorus fertilized 2 and salinity of 2.5 dS.m-1 led to the highest average length of seedling vigor index (1375.83), root length (6.83 cm) and seedling length (14 cm).The highest mean time to germination (2.15), weight seedling vigor index (1.87) and dry weight (0.019 g) was conducted to priming with Pseudomonas fluorescent in salinity of 10 dS.m-1. Non-priming (control) in zero levels of salinity (control) also has the highest mean germination rate (15.33 seeds per day).The use of biological phosphorus fertilized 2 and Pseudomonas fluorescent is effective on modified germination index of flaxseed  especially increased seedling growth and seed vigor in salinity stress.

کلیدواژه‌ها [English]

  • Azotobacter
  • Biological phosphorus fertilized 2
  • Flax Seed
  • Germination
  • Pseudomonas
  • Salinity
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