بررسی کارایی سه روش پیش‌تیمار بذر درتحمل به تنش شوری و خشکی گلرنگ (Cartthamus tinctorius L.) در مرحله جوانه‌زنی و رشد اولیه

نوع مقاله: مقاله پژوهشی

نویسندگان

1 عضو هیئت علمی گروه علوم کشاورزی، دانشگاه پیام نور، تهران، ایران

2 استادیار گروه علوم کشاورزی، دانشگاه پیام نور، تهران، ایران

چکیده

به­منظور بررسی کارایی سه روش پیش­تیمار بذر جهت افزایش مقاومت به تنش‌های شوری و خشکی گلرنگ در مرحله جوانه‌زنی و رشد اولیه، آزمایشی به صورت فاکتوریل در قالب طرح کاملا تصادفی و در چهار تکرار در آزمایشگاه موسسه تحقیقات گیاهپزشکی کشور، بخش علف‌‌های‌هرز انجام گرفت. عامل اول نوع تنش شامل دو تنش شوری و خشکی، عامل دوم روش پیش‌تیمار بذر شامل سه روش هیدروپرایمینگ (پیش­تیمار بذر با استفاده از آب مقطر به مدت 6 ساعت)، هالوپرایمینگ (تیمار بذر در محلول کلرید سدیم 100میلی­مولار) و پرایمینگ با استفاده از KNO3 (5/0 درصد) به همراه شاهد (عدم پیش‌تیمار) و عامل سوم پتانسیل آب شامل 0، 3/0-، 6/0- و 2/1- مگاپاسکال بود که جهت ایجاد پتانسیل‌های مذکور در تنش شوری از NaCl و در تنش خشکی از PEG (پلی اتیلن گلایکول) استفاه گردید. نتایج نشان داد که هر دو تنش خشکی و شوری بر روی جذب آب، درصد جوانه‌زنی، طول ریشه و ساقه و شاخص بنیه گیاهچه اثر منفی گذاشت ولی در پتانسیل رطوبتی برابر، تاثیر منفی تنش خشکی بر کلیه صفات به­صورت معنی‌دار بیشتر از تنش شوری بود. در همه پتانسیل­های رطوبتی و به­ویژه در مورد تنش خشکی اثر پیش­تیمار بذر بر حفظ توان جوانه­زنی بذر موثر بود. تاثیر مثبت پیش­تیمار بذر در هر دو تنش خشکی و شوری، با شدیدتر شدن تنش افزایش یافت. در هر دو تنش خشکی و شوری تاثیر هیدروپرایمینگ بذر بر کاهش خسارت تنش­ها بیشتر از سایر پیش­تیمارهای مورد آزمایش بود و می­تواند به عنوان راهکاری کم­هزینه و سودمند به کار گرفته شود.

کلیدواژه‌ها


عنوان مقاله [English]

Study the Efficiency of Three Seed Priming Methods for Salt and Drought Stresses Tolerance of Safflower (Cartthamus tinctorius L.) in Germination and Seedling Stags

نویسندگان [English]

  • Leila Jahanban 1
  • Omid Lotfifar 2
  • Samaneh Mottaghi 2
چکیده [English]

To study the efficiency of three priming methods to increase salt and drought stress resistant of safflower in germination and early growth, a laboratory experiment was conducted as factorial arranged in completely randomized design with four replications in seed laboratory of Iranian Plant Protection Research Institute. First factor was kind of stress include salt and drought stress, second factor was priming method include hydropriming (treated with water for 6 h), Halopriming (treated with NaCl 100 mM), KNO3 (0.5%) and no priming as control and third factor was water potential include 0.0, -0.3, -0.6, -0.9 and -1.2 MPa. Too, salt and drought conditions caused by NaCl and PEG respectively. Results showed that both stresses inhibited water uptake, germination percentage, radicle and Plumule length and seedling vigour index, but this criteria significantly were higher in NaCl than PEG at the same water potential. All of three used priming treatments increased germination and seedling growth under salt and drought stresses. By increase of stress intensity, efficiency of priming methods increased too. Hydropriming was more effective than other priming to decrease salt and drought damage in germination and early growth stages and it is suitable and low cost strategy.

کلیدواژه‌ها [English]

  • Drought stress
  • Germination rate
  • Priming
  • Safflower
  • Salt stress
Abdul-Baki, A.A. and Anderson, J.D. 1973. Relationship between decarboxylation of glutamic acid and vigour in soybean seed. Crop Science, 13: 227-232. (Journal)

Afzal, I., Ashraf, S., Qasim, M., Basra, S.M.A. and Shahid, M. 2009. Does halopriming improve germination and seedling vigour in marigold (Tagetesspp.). Seed Science and Technology, 37: 436-445. (Journal)

Akinola, J.O., Larbi, A., Farinu, G.O. and Odunsi, A.A. 2000. Seed treatment methods and duration effects on germination of wild sunflower. Experimental Agriculture, 36: 63–69. (Journal)

Alizadeh, A. 2001. Practical Hydrology principles. Astan Ghods Razavi Press. (In Persian)(Book)

Ashrafi, E. and Razmjoo, K. 2010. Effects of priming on seed germination and field emergence of safflower (Carthamus tinctorius L.). Seed Science and Technology, 38: 675-681. (Journal)

Basra, S.M.A., Farooq, M., Hafeez, K. and Ahmad, N. 2004. Osmohardening: A new technique for rice seed in vigour action. International Rice Research Notes, 29: 80-81. (Journal)

Bijanzadeh, E., Nosrati, K. and Egan, T. 2010. Influence of seed priming techniques on germination and emergence of rapeseed (Brassica napus L.). Seed Science and Technology, 38: 242-247. (Journal)

Caseiro, R., Bennett, M.A. and Marcos-Filho, J. 2004. Comparison of three priming techniques for onion seed lots differing in initial seed quality. Seed Science and Technology, 32: 365–375. (Journal)

Casenave, E.C. and Toselli, M.E. 2010. Germination of melon seeds under water and heat stress: Hydropriming and the hydrotime model. Seed Science and Technology, 38: 409-420. (Journal)

Cheng, Z. and Bradford, K.J. 1999. Hydrothermal time analysis of tomato seed germination responses to priming treatments. Journal of Experimental Botany, 50: 89-99. (Journal)

Demir, I. and Ermis, S. 2003. Effect of controlled hydration treatment on germination and seedling growth under salt stress during development in tomato seeds. European Journal of Horticulture Science, 68: 53–58. (Journal)

Demir, I. and Van De Venter, H.A. 1999. The effect of priming treatments on the performance of watermelon (Citrillus lanatus (Thunb.) Matsum and Nakai) seeds under temperature and osmotic stress. Seed Science and Technology, 27: 871–875. (Journal)

Farhoudi, R., Sharifzadeh, F., Poustini, K., Makkizadeh, M.T. and Kochakpor, M. 2007. The effects of NaCl priming on salt tolerance in canola (Brassica napus) seedlings grown under saline conditions. Seed Science and Technology, 35: 754-759. (Journal)

Farooq, M., Basra, M.S.A., Hafeez, K. and Ahmad, N. 2005. Thermal hardening: a new seed vigour enhancement tool in rice. Journal of Integrated Plant Biology, 47: 187-193. (Journal)

Farooq, M., Basra, S.M.A., Rehman, H. and Saleem, B.A. 2008. Seed priming enhances the performance of wheat (Triticum aestivum L.) by improving chilling tolerance late sown. Journal of Agronomy and Crop Science, 94: 55-60. (Journal)

Farzadmehr, J., Ramezani. M., Behbahani, N. and Moeini, N. 2011. The effect of salinity and drought stress on germination characteristics and seedling growth of Salsola arbuscula. Iranian Natural Resources, 64 (2): 217-227. (In Persian)(Journal)

Ghassemi-Golezani, K., Sheikhzadeh-Mosaddegh, P. and Valizadeh, M. 2008. Effects of hydropriming duration and limited irrigation on field performance of chickpea. Journal of Seed Science and Research, 1: 34-40. (In Persian)(Journal)

Grundy, A.C., Phelps, K., Reader, R.J. and Burston, S. 2000. Modeling the germination of Stellaria media using the concept of hydrothermal time. New Phytology, 148: 433- 444. (Journal)

Guerke, W.R., Gutormson, T., Meyer, D., McDonald, M., Mesa, D., Robinson, J.C. and TeKrony, D. 2004. Application of hydro-time analysis in seed testing. Seed Science and Technology, 26: 75-85. (Journal)

Hadas, A. 1977. A simple laboratory approach to test and estimate seed germination performance under field conditions. Agronomy Journal, 69: 582-588. (Journal)

Harris, D., Tripathi, R.S. and Joshi, A. 2000. On-farm seed priming to improve crop establishment and yield in direct-seeded rice. Proceedings of the International Workshop on Dry-seeded Rice Technology, 25-28 January; Manila, Bangkok, Thailand. pp. 383. (Conference)

Hosseini, H. and Rezvani-Moghaddam, P. 2006. The effect of drought and salt stress on fleawort. Iranian Journal of Field Crops Research, 4: 15-23. (Journal)

Hu, J., Xie, X.J., Wang, Z.F. and Song, W.J. 2006. Sand priming improves alfalfa germination under high-salt concentration stress. Seed Science and Technology, 34: 199-204. (Journal)

Jajarmi, V. 2008. Effect of water stress on germination indices in seven safflower cultivars (Carthamus tinctorius L.). Proceedings of the 7th International safflower Conference. November 3-6.New South Wales, Australia. pp. 1–3. (Conference)

Kaya, M.D., Okc, U.G., Atak, M.C., Ikili, Y. and Kolsarici, O. 2006. Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annus L.). European Journal of Agronomy, 24: 291–295. (Journal)

Khajeh-Hosseini, M., Powell, A.A. and Bingham, I.J. 2003. The interaction between salinity stress and seed vigour during germination of soybean seeds. Seed Science and Technology, 31: 715–725. (Journal)

Li, J., Yinb, L.Y., Jongsmac, M.A. and Wang, C.Y. 2011. Effects of light, hydropriming and abiotic stress on seed germination, and shoot and root growth of pyrethrum (Tanacetum cinerariifolium). Industrial Crop Production, 34: 1543– 1549. (Journal)

Mittler, R. and Zilinskas, B.A. 1994. Regulation of pea cytosolic ascorbate peroxidase and other antioxidant enzymes during the progression of drought stress and following recovery from drought. Plant Journal, 5: 397-405. (Journal)

Mojab, M., Zamani, Gh.R., Eslami, S.V., Hoseini, M. and Naseri, A. 2010. Study the effect of salt and drought stresses caused by NaCl and PEG on Pigweed (hinochloa crus-galliVar:oryzicola). Journal of Plants Production, 24: 108-104. (Journal)

Murillo-Amador, B., Lopez-Aguilar, R., Kaya, C., Larrinaga-Mayoral, J. and Flores-Hernandez, A. 2002. Comparative effects of NaCl and polyethylene glycol on germination, emergence and seedling growth of cowpea. Journal of Agronomy and Crop Science, 188: 235–247. (Journal)

Netondo, G.W., Onyango, J.C. and Beck, E. 2004. Sorghum and salinity: I. Response of growth, water relation, and ion accumulation to NaCl salinity. Crop Science, 44: 797-805. (Journal)

Nirmala, K. and Umarani, R. 2007. Evaluation of seed priming methods to improve seed vigour of okra (Abelmoschus esculentus) and beetroot (Beta vulgaris). Seed Science and Technology, 36: 56-65. (Journal)

Okcu, G., Kaya, M.D. and Atak, M. 2005. Effects of salt and drought stresses on germination and seedling growth of pea (Pisum sativum). Turkish Journal of Agriculture and Forestly, 29: 237-242. (Journal)

Patane, C., Cavallaro, V. and Cosentino, S.L. 2009. Germination and radicle growth in unprimed and primed seeds of sweet sorghum as affected by reduced water potential in NaCl at different temperatures. Industrial Crop Production, 30: 1–8. (Journal)

Srinivasan, K., Saxena, S. and Singh, B.B. 1999. Osmo- and hydropriming of mustard seeds to improve vigour and some biochemical activities. Seed Science and Technology, 27: 785–793. (Journal)

Tazikeh, N., Dadashi, M.R. and Jafari, M.J. 2013. Study of germination two wheat cultivars under salt and drought stress. Journal of Seed Science and Research, 3 (4): 1-7. (In Persian)(Journal)

Yagmur, M. and Kaydan, D. 2008. Alleviation of osmotic stress of water and salt in germination and seedling growth of triticale with seed priming treatments. African Journal of Biotechnology, 7: 2156–2162. (Journal)