اثر برهمکنش روش‌های خراش‌دهی سنتی و امواج فراصوت در ترکیب با جیبرلین بر شکست خواب بذر و رشد دانه‌رست گون گزی (Astragalus adscendens Boiss. & Hausskn)

مقالات آماده انتشار

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم گیاهی، دانشکده علوم دانشگاه شهرکرد

2 گروه علوم گیاهی، دانشکده علوم، دانشگاه شهرکرد

10.22124/jms.2026.9717

چکیده

تکثیر گیاه گون گزی بدلیل پوسته سخت بذرهای آن مشکل است. بنابراین در مطالعه حاضر، در آزمایش اول اثر خراش­دهی فیزیکی و شیمیایی (شاهد، 2 و 5 دقیقه سنباده­زنی بذر و غوطه­وری در اسید کلریدریک با غلظت 50 درصد به مدت 2، 5 و10 دقیقه) بر جوانه­زنی بذر بررسی شد. بهترین نتایج با 2 دقیقه سنباده و 5 دقیقه غوطه­وری در اسید بدست آمد که منجر به حدود 70 درصد جوانه­زنی و بیشترین مقادیر طول دانه­رست و بنیه بذر شد. در آزمایش دوم اثر امواج فراصوت با سطوح مختلف قدرت (0، 25، 50 و 100 وات) و مدت زمان (5، 10 و 15 دقیقه) روی شکست خواب بذر ارزیابی شد. نتایج نشان داد بیشترین درصد جوانه­زنی (%75)، کمترین میانگین زمان جوانه­زنی (5 روز) و بالاترین طول دانه­رست (46 میلی­متر) و بنیه (33 میلی­متر) با استفاده از توان فراصوت 50 وات به مدت 15 دقیقه به­دست آمد. در حالی­که با افزایش توان به 100 وات در مدت زمان 15 دقیقه، این پارامترها به شدت کاهش یافتند. در آزمایش سوم، اثر متقابل بهترین سطوح خراش­دهی هر دو آزمایش فوق با سطوح مختلف جیبرلین بر شکست خواب بذر و رشد دانه­رست گون گزی بررسی شد. بهترین نتایج با تیمار ترکیبی فراصوت و 1000 میلی­گرم در لیتر جیبرلین بدست آمد که منجر به 95 درصد جوانه­زنی و بیشترین طول ریشه (27 میلی­متر) و ساقه­چه (23 میلی­متر) دانه ­رست شد. این یافته­ها، پیشنهاد می­کند 70 درصد خواب بذر گون گزی از نوع فیزیکی بوده و تنها حدود 25 درصد از خواب بذر از نوع فیزیولوژیکی است.

کلیدواژه‌ها

عنوان مقاله [English]

The effects of combining traditional scarification methods and ultrasonic waves with gibberellin on seed dormancy breaking and seedling growth in Astragalus adscendens Boiss. & Hausskn

نویسندگان [English]

  • Reyhaneh Amooaghaie 1
  • Negin Moein 2
1 Plant Science Department, Faculty of Science, Shahrekord University, Shahrekord, Iran
2 Plant Science Department, Faculty of Science, Shahrekord University, Shahrekord, Iran
چکیده [English]

The propagation of Astragalus adscendens Boiss. & Hausskn is challenging due to its hard seed coat. Therefore, in the present study, in the first experiment, the effect of physical and chemical scarification methods (control, 2 and 5 min scarification with sand paper, and 2, 5, and 10 min treatment with 50% HCl) was investigated. Optimal results were achieved with 2 min sanding and 5 min immersion in acid, which led to approximately 70% germination and the highest seedling length and vigor. In the second experiment, the impact of ultrasonic waves at varying power levels (0, 25, 50, and 100 w) and durations (5, 10, and 15 min) on breaking seed dormancy was evaluated. The results showed that the shortest mean germination time (5 days), and the highest germination (75%), seedling length (46 mm) and vigor (33) were obtained by exposing to 50 w power of ultrasound for 15 min. However, increasing the power to 100 w especially for 15 min duration significantly reduced these parameters. In the third experiment, the interaction effects of the optimal scarification treatments from two aforementioned experiments and different gibberellin concentrations were studied. The best results were obtained with the combined treatment of ultrasound and 1000 mg/L gibberellin, achieving a germination rate of 95%, along with the highest root length (27 mm) and shoot length (23 mm) in seedlings. These findings suggest that approximately 70% of A. adscendens seed dormancy is physical type, and only 25% is attributed to physiological dormancy.

کلیدواژه‌ها [English]

  • Mechanical and chemical scarification
  • Physiological dormancy
  • Seed germination velocity
  • Seedling vigor

مراجع

Abdali, N.S., Aliniaeifard, M., Nasiri, Salavarzian, A. and Azizi, F. 2010. The study of ultrasonic waves on seed germination of Ferula (assa-foetida). International Medical and Aromatic Plants Symposium, Shiraz-Iran.5 p. (Conference)
Alvandian. S., Vahedi, A. and Taghi-Zadh, R. 2014. The effect of ultrasound and chilling on seed germination of medicinal plants (Myrtus communis L.). Journal of Seed Research, 3(3), 21-31. (In Persian)(Journal)
Amooaghaie, R., and Khodadadi, A. 2025. Interaction of various scarification methods with cold stratification and gibberellin on dormancy breaking and growth of Gundelia tournefortii L.. Iranian Journal of Seed Research, 11(2):77 -94. http://dx.doi.org/10.61882/yujs.11.2.77 (In Persian)(Journal)
Amooaghaie, R., and Rafiei, A. 2025. The role of GA and ABA in moist chilling-induced effects on germination of scarified seeds of Myrtus commnius L.. Journal of Plant Research (Iranian Journal of Biology), 38(4), 359-372. (In Persian)(Journal)
Andriamparany, J.N., Buerkert, A. 2019. Effect of ultrasonic dormancy breaking on seed germination and seedling growth of three wild yam species (Dioscorea spp.) from SW-Madagascar. Genetic Resources and Crop Evolution 66, 1167–1174. https://doi.org/10.1007/s10722-019-00779-5. (Journal)
Asadi-Gakieh, M., Babaei-Ghaghelestany, A., Ghazvineh, M. 2022. Evaluation of different methods of seed dormancy breaking in Alyssum homalocarpum weed. Journal of Seed Research, 11(4), 15-22. (In Persian)(Journal)
Babaei-Ghaghelestany, A., Alebrahim, M.T., MacGregor, D.R., Khatami, S.A., Hasani Nasab Farzaneh, R. 2020. Evaluation of ultrasound technology to break seed dormancy of common lambsquarters (Chenopodium album). Food Science and Nutrition. 8: 2662–2669. (Journal)
Baskin, C.C. and Baskin, J.M. 2014. Seeds: ecology, biogeography, and evolution of dormancy and germination (2nd edition). New York, USA, Academic Press.
Beyaz, R., C.T, Kahramanogullari., C, Yildiz., E.S, Darcin, and M, Yildiz. 2016. The effect of gamma radiation on seed germination and seedling growth of Lathyrus chrysanthus Boiss. under in vitro conditions. Journal of Environmental Radioactivity. 162: 129-133. (Journal)
Chen, J., Shao, F., Igbokwe, C. J., Duan, Y., Cai, M., Ma, H., and Zhang, H. 2023. Ultrasound treatments improve germinability of soybean seeds: The key role of working frequency. Ultrasonics Sonochemistry, 96: 106434. (Journal)
Eisvand, H.R. and Latifinia, E. 2020. Effects of hydropriming and ultrasonic waves on seed leakage and germination of Artemisia (Artemisia aucheri L.). Iranian Journal of Seed Science and Research, 7(2): 179-190. (In Persian)(Journal)
Foschi, M.L., Juan, M., Pascual, B., Pascual-Seva, N. 2023. Effects of high intensity ultrasound stimulation on the germination performance of Caper seeds. Plants (Basel). 12(12): 2379. (Journal)
Hasani, A., Khoshtaghaza, M.H. and Ebad, M.T. 2021. Effect of ultrasound on seed germination factors of Iranian black cumin (Bunium persicum Boiss.) and its optimization. Iranian Journal of Seed Science and Technology, 10(2), 37-52. (In Persian)(Journal)
Haidarian, M., Tamartash, R., Jafarian, Z., Tarkesh, M., and Tatian, M. 2021. The effects of climate changes on the future distribution of Astragalus adscendens in Central Zagros, Iran. Journal of Rangeland Science, 11(2), 152- 170. (In Persian)(Journal)
Huang, S., Ashraf, U., Duan, M., Ren, Y., Xing, P., Yan, Z., and Tang, X. 2024. Ultrasonic seed treatment improved seed germination, growth, and yield of rice by modulating associated physio-biochemical mechanisms. Ultrasonics Sonochemistry, 104: 106821. (Journal)
Jaganathan, G. K., Li, J., Biddick, M., Han, K., Song, D., Yang, Y., Han, Y. and Liu, B. 2019. Mechanisms underpinning the onset of seed coat impermeability and dormancy-break in Astragalus adsurgens. Scientific Reports. 9: 9695. (Journal)
Keshtkar, A. R., Keshtkar, H. R., Razavi, S. M., and Dalfardi, S. 2008. Methods to break seed dormancy of Astragalus cyclophyllon. African Journal of Biotechnology, 7(21): 3874-3877. (Journal)
Klupczyńska, E. A., and Pawłowski, T. A. 2021. Regulation of seed dormancy and germination mechanisms in a changing environment. International Journal of Molecular Sciences, 22(3): 1357. (Journal)
Khodadadi, A. and Amooaghaie, R. 2024. The effect of scarification and temperature shock on seed germination and seedling length of Gundelia tournefortii L.. Iranian Journal of Seed Science and Technology, 13 (4), 81-96. (In Persian)(Journal)
Liu, J., Wang, Q., Karagić, Đ., Liu, X. V., Cui, J., Gui, J. Gu, M., Gao, W. 2016. Effects of ultrasonication on increased germination and improved seedling growth of aged grass seeds of tall fescue and Russian wildrye. Scientific Reports. 6: 22403. (Journal)
Maleki-Farahani, S., Reza-Zade, A.R. and Aghili-Shahrodi, M. 2015. The Effect of magnetic field and ultrasound on the seed germination (Cuminum cyminum L.). Iranian Journal of Seed Reseach, 2(1): 110-118. (In Persian)(Journal)
Martinez-Fernandez, V., Martinez-Garcia, F., Perez-Garcia, F. 2014.  Census reproductive biology, and germination of Astragalus gines-lopezii (Fabaceae), a narrow and endangered endemic species of SW Spain. Turkish Journal Botany. 38(4): 686–695. (Journal)
Mansouri, Sh., Ghahsareh Ardestani, E., and Khedri Gharibvand, H. 2022. Astragalus adscendens as a predictor of rangeland ecosystem functions and services in the Zagros territory residents of Bakhtiari. Studies of Nomads Area Plannig, 1(2), 1-10. (In Persian)(Journal)
Maguire, J. D. 1962. Speed of germination-aid selection and evaluation for seedling emergence and vigor. Crop Science. 2: 176-177. (Journal)
Miano, A. C., Forti, V. A., Abud, H. F., Gomes-Junior, F. G., Cicero, S. M., Augusto, P. E. D. 2015. Effect of ultrasound technology on barley seed germination and vigour. Seed Science and Technology. 43(2): 297–302. (Journal)
Mokhobo, M.M. 2018. Evaluation of scarification and stratification techniques in breaking seed dormancy and development of a regeneration protocol for Rosa Rubiginosa (Doctoral dissertation, JKUAT-PAUSTI).
Mehrabi, A.A., and Hajinia, S. 2019. The effect of seed pre -treatments on germination of Astragalus gossypinus Seed. Iranian Journal of Seed Research, 6(1): 95 -113. (In Persian)(Journal)
Nguyen, T. N., Tuan, P. A., and Ayele, B. T. 2022. Jasmonate regulates seed dormancy in wheat via modulating the balance between gibberellin and abscisic acid. Journal of Experimental Botany, 73(8): 2434-2453. (Journal)
Patero, T., Augusto, P. E. 2015. Ultrasound (US) enhances the hydration of sorghum (Sorghum bicolor) grains. Ultrasonics Sonochemistry. 23: 11–15. (Journal)
Statwick, J.M. 2016. Germination pretreatments to break hard-seed dormancy in Astragalus cicer L. (Fabaceae). PeerJ. 4: 2621. (Journal)
Rasouli, F., Gholipoor, M., Jahanbin, K., and Asghari, H.R.. 2019. Ultrasonic waves effect on germination, α amylase activity, antioxidant enzymes, sugars and chlorophyll on Echinacea purpurea L.. Journal of PLANTlant Production, 26(3), 21-36. (In Persian)(Journal)
Soltani, E., Baskin, J.M., Baskin, C.C., and Benakashani, F. 2020. A meta-analysis of the effects of treatments used to break dormancy in seeds of the megagenus Astragalus (Fabaceae). Seed science research, 30(3): 224-233. (In Persian)(Journal)
Salehi-Eskandari, B., and Kavian, M. 2021. The Evaluation of methods on dormancy breaking and some characters of germination in four species of Astragalus.sp. Iranian Journal of Seed Science and Technology, 9(4): 99-110. (In Persian)(Journal)
Sharififar, A., Nazari, M., Asghari, H.R. 2015. Effect of ultrasonic waves on seed germination of Atriplex lentiformis, Cuminum cyminum, and Zygophyllum eurypterum. Journal of Applied Research on Medicinal and Aromatic Plants. 2(3): 102–104. (In Persian)(Journal)
Shu, K.,  Liu, X.,  Xie, Q. and He, Z. 2015. Two facea of one seed: Hormonal regulation of dormancy and germination. Molecular Plant. 9: 34-45. (Journal)
Venâncio, R.S.D.S., and Martins, A.C.G. 2019. Overcoming dormancy of Senna multijuga seeds with an ultrasonic probe the comparison with ultrasound and sulfuric acid baths. Ciência rural, 49.
Yaldagard, M., Mortazavi, S. A., Tabatabaie, F. 2008. Influence of ultrasonic stimulation on the germination of barley seed and its alpha-amylase activity. African Journal of Biotechnology. 7(14): 2465–2471. (Journal)
Zhang, H., Mo, W., Liao, S., Jia, Z., Zhang, W., Zhang, S., and Liu, Z. 2023. Ultrasound promotes germination of aging Pinus tabuliformis seeds is associated with altered lipid metabolism. Ultrasonics Sonochemistry, 93: 106310. (Journal)
علوم و تحقیقات بذر ایران

مقالات آماده انتشار، پذیرفته شده
انتشار آنلاین از تاریخ 31 خرداد 1405

فایل ها

هم رسانی

ارجاع به این مقاله

آمار