تغییرپذیری پرولین، قندهای محلول و آنزیمهای آنتی‌اکسیدانی گیاهچه سویا در واکنش به رژیم‌های مختلف آبیاری و گونه‌های مایکوریزا

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی دانشگاه کردستان، سنندج، ایران

2 گروه مهندسی تولید و ژنتیک گیاهی دانشکده کشاورزی دانشگاه کردستان سنندج ایران

3 گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه کردستان سنندج، ایران

4 استاد گروه زراعت و اصلاح نباتات دانشگاه کردستان

10.22124/jms.2026.9715

چکیده

تنش خشکی تعادل اسمزی و پایداری اکسیداتیو سویا را به‌طور جدی مختل می‌کند، اما نقش گونه‌محور قارچ‌های مایکوریزا آربسکولار (AMF) در تنظیم این فرآیندها تحت رژیم‌های مختلف آبیاری به‌طور کامل تبیین نشده است. این پژوهش در سال ۱۴۰۳ به‌صورت فاکتوریل ۳×۳ در قالب طرح کاملاً تصادفی با سه تکرار اجرا شد. تیمارهای آزمایش شامل رژیم‌های آبیاری در سه سطح (۱۰۰٪ ظرفیت زراعی به‌عنوان شاهد، ۸۰٪ ظرفیت زراعی به‌عنوان تنش کم‌آبی متوسط و ۵۰٪ ظرفیت زراعی به‌عنوان تنش کم‌آبی شدید) و کاربرد گونه‌های مایکوریزا (شاهد، Funneliformis mosseae و (Rhizophagus intraradices بود. اثر این تیمارها بر پرولین، قندهای محلول، محتوای نسبی آب (RWC)، مالون‌دی‌آلدئید (MDA) و فعالیت آنزیم‌های آنتی‌اکسیدانی گیاهچه سویا مورد ارزیابی قرار گرفت. کاهش آبیاری در سطوح 80 و 50 درصد ظرفیت مزرعه موجب افت معنی‌دار RWC و افزایش تجمع اسمولیت‌ها، پراکسیداسیون چربی و فعالیت آنتی‌اکسیدانی شد. تلقیح با AMF شدت این پاسخ‌ها را کاهش داد و Rhizophagus intraradices مؤثرترین عملکرد را در بهبود وضعیت آبی، تنظیم اسمزی و کاهش آسیب اکسیداتیو به‌ویژه در خشکی متوسط و شدید نشان داد. این نتایج حاکی از آن است که AMF، به‌ویژه Rhizophagus intraradices، از طریق بهبود وضعیت آبی، تجمع اسمولیت‌ها و کاهش آسیب اکسیداتیو در افزایش تحمل خشکی سویا نقش دارد. در مجموع، پژوهش حاضر شواهد جدیدی از کارآیی برتر Rhizophagus intraradices ارائه می‌دهد و استفاده از این گونه را به‌عنوان راهبردی پایدار برای مدیریت خشکی در کشت سویا پیشنهاد می‌کند.

کلیدواژه‌ها

عنوان مقاله [English]

Variations in proline, soluble sugars, and antioxidant enzymes of soybean in response to different irrigation regimes and mycorrhiza treatments

نویسندگان [English]

  • Nasrin Ghamarirahim 1
  • Zahra Najafivafa 2
  • Adel Siosemardeh 3
  • Yousef Sohrabi 4
1 Department of Plant production and Genetic, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran
2 Department of Plant production and Genetic, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran.
3 Department of Plant production and Genetic, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran.
4 Department of Plant production and Genetic, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran
چکیده [English]

Drought stress severely disrupts osmotic balance and oxidative stability in soybean; however, the species-specific role of arbuscular mycorrhizal fungi (AMF) in regulating these processes under different irrigation regimes has not been fully clarified. This study was conducted in 2024 as a 3 × 3 factorial experiment based on a completely randomized design with three replications. Experimental treatments included three irrigation regimes (100% field capacity as the control, 80% field capacity as moderate drought stress, and 50% field capacity as severe drought stress) and three mycorrhizal treatments (non‑inoculated control, Funneliformis mosseae, and Rhizophagus intraradices). The effects of these treatments on proline, soluble sugars and relative water content (RWC), malondialdehyde (MDA), and antioxidant enzyme activity in soybean seedlings were evaluated.
Reducing irrigation to 80% and 50% of field capacity significantly decreased RWC and increased osmolyte accumulation, lipid peroxidation, and antioxidant enzyme activity. Inoculation with AMF mitigated these stress responses, and Rhizophagus intraradices showed the most pronounced effect in improving plant water status, regulating osmotic adjustment, and reducing oxidative damage, particularly under moderate and severe drought conditions. These results indicate that AMF, especially Rhizophagus intraradices, contribute to enhanced drought tolerance in soybean through improved water status, increased osmolyte accumulation, and reduced oxidative damage. Overall, the findings provide evidence of the superior performance of Rhizophagus intraradices in alleviating drought stress in soybean.

کلیدواژه‌ها [English]

  • Drought stress
  • arbuscular mycorrhizal fungi (Funneliformis mosseae
  • and Rhizophagus intraradices)
  • lipid peroxidation (malondialdehyde)
  • relative water content

مراجع

  1. Abbaspour, H., Saeidi, G. and Afshari, R. 2018. Arbuscular mycorrhiza improves water status and yield of wheat under drought stress. Archives of Agronomy and Soil Science, 64(8), 1102–1115. https://doi.org/10.1080/03650340.2017.1412949 (Journal)
  2. Aebi, H. 1984. Catalase in vitro. In L. Packer (Ed.). Methods in Enzymology, Vol. 105, 121–126. Academic Press. https://doi.org/10.1016/S0076-6879(84)05016-3 (Journal)
  3. Ahmad, P., Jaleel, C. A., Salem, M. A., Nabi, G. and Sharma, S. 2021. Roles of enzymatic and non-enzymatic antioxidants in plants during abiotic stress. Physiologia Plantarum, 172(2), 102–119. https://doi.org/10.1111/ppl.13243 (Journal)
  4. Arnon, D. I. 1949. Copper enzymes in isolated chloroplasts: Polyphenoloxidase in Beta vulgaris. Plant Physiology, 24(1), 1–15. https://doi.org/10.1104/pp.24.1.1 (Journal)
  5. Barrs, H. D. and Weatherley, P. E. 1962. A re-examination of the relative turgidity technique for estimating water deficits in leaves. Australian Journal of Biological Sciences, 15(3), 413–428. https://doi.org/10.1071/BI9620413 (Journal)
  6. Bárzana, G., Aroca, R., Bienert, G. P., Chaumont, F. and Ruiz-Lozano, J. M. 2015. Arbuscular mycorrhizal symbiosis increases relative apoplastic water flow in maize roots under drought stress. Frontiers in Plant Science, 6, 787. https://doi.org/10.3389/fpls.2015.00787 (Journal)
  7. Bates, L. S., Waldren, R. P. and Teare, I. D. 1973. Rapid determination of free proline for water-stress studies. Plant and Soil, 39(1), 205–207. https://doi.org/10.1007/BF00018060 (Journal)
  8. Beauchamp, C. and Fridovich, I. 1971. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry, 44(1), 276–287. https://doi.org/10.1016/0003-2697(71)90370-8 (Journal)
  9. Begum, N., Qin, C., Ahanger, M. A., Raza, S., Khan, M. I., Ashraf, M., Ahmed, N. and Zhang, L. 2019. Role of arbuscular mycorrhizal fungi in plant growth regulation: Implications in abiotic stress tolerance. Frontiers in Plant Science, 10, 1068. https://doi.org/10.3389/fpls.2019.01068 (Journal)
  10. Brundrett, M., & Tedersoo, L. 2018. Evolutionary history of mycorrhizal symbioses and global host plant diversity. New Phytologist, 220(4), 1108–1115. https://doi.org/10.1111/nph.14976 (Journal)
  11. Cakmak, I. and Horst, W. J. 1991. Effect of aluminum on lipid peroxidation, superoxide dismutase, catalase and peroxidase activities in root tips of soybean (Glycine max). Physiologia Plantarum, 83, 463–468. https://doi.org/10.1111/j.1399-3054.1991.tb00121.x (Journal)
  12. Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. and Smith, F. 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28(3), 350–356. https://doi.org/10.1021/ac60111a017 (Journal)
  13. Estrada, B., Barea, J. M. and Azcón-Aguilar, C. 2013. Arbuscular mycorrhiza fungi alleviate oxidative stress in lettuce plants grown under different light intensities. Plant Science, 198, 72–81. https://doi.org/10.1016/j.plantsci.2012.10.008 (Journal)
  14. Farooq, M., Hussain, M. and Siddique, K. H. M. 2019. Drought stress in wheat during flowering and grain filling periods. Critical Reviews in Plant Sciences, 38(3), 190–205. https://doi.org/10.1080/07352689.2019.1575679 (Journal)
  15. Food and Agriculture Organization (FAO). (2023). The state of the world’s land and water resources for food and agriculture 2023. FAO. https://doi.org/10.4060/cb9910en (Journal)
  16. Gee, G. W. and Bauder, J. W. 1986. Particle-size analysis. In A. Klute (Ed.), Methods of Soil Analysis: Part 1. Physical and Mineralogical Methods, 2nd ed., 383–411. ASA and SSSA. https://doi.org/10.2136/sssabookser5.1.2ed.c15 (Journal)
  17. Hasanuzzaman, M., Bhuyan, M. H. M. B., Zulfiqar, F., Raza, A., Mohsin, S. M., Mahmud, J. A., Fujita, M. and Fotopoulos, V. 2020. Reactive oxygen species and antioxidant defense in plants under abiotic stress. Antioxidants, 9(8), 681. https://doi.org/10.3390/antiox9080681 (Journal)
  18. Hosseini, S. A., Prasad, P. V. V. and Djanaguiraman, M. 2020. Osmolyte accumulation in plants under abiotic stress: mechanisms and perspectives. Plant Physiology and Biochemistry, 151, 1–10. https://doi.org/10.1016/j.plaphy.2020.03.005 (Journal)
  19. Kiani, H. and Sohrabi, Y. 2022. Effects of controlled irrigation on soybean physiology under greenhouse conditions. Journal of Plant Stress Physiology, 12(3), 45–58. (Journal)
  20. Laxa, M., Liebthal, M., Telman, W., Chibani, K. and Dietz, K. J. 2019. The role of the plant antioxidant system in drought tolerance. Antioxidants, 8(4), 94. https://doi.org/10.3390/antiox8040094 (Journal)
  21. Li, M., Guo, R., Yu, F., Chen, X., Zhao, H., Li, H. and Wu, J. 2022. Arbuscular mycorrhiza fungi enhance drought tolerance in soybean by regulating osmolytes and antioxidant enzymes. Agronomy, 12(8), 1764. https://doi.org/10.3390/agronomy12081764 (Journal)
  22. Li, T., Hu, Y., Hao, Z., Li, H., Wang, Y. and Chen, B. 2019. Arbuscular mycorrhiza symbiosis improves photosynthesis and water status of maize under drought stress. Plant and Soil, 439, 567–583. https://doi.org/10.1007/s11104-019-04062-1 (Journal)
  23. Mittler, R. 2022. ROS signaling in plant stress responses. Annual Review of Plant Biology, 73, 299–328. https://doi.org/10.1146/annurev-arplant-102820-012655 (Journal)
  24. Najafi vafa, Z., Sohrabi, Y., Mirzaghaderi, GH. and Heidari, GH. 2022a. Soil Microorganisms and Seaweed Application with Supplementary Irrigation Improved Physiological Traits and Yield of Two Dryland Wheat Cultivars. Frontiers in. Plant Science, 13, pp. 1-23. https://doi.org/10.3389/fpls.2022.855090 (Journal)
  25. Najafi vafa, Z., Sohrabi, Y., Mirzaghaderi, GH., Heidari, GH., Rizwan, M. and Sayyed, R.Z. 2024. Effect of bio-fertilizers and seaweed extract on growth and yield of wheat (Triticum aestivum ) under different irrigation regimes: Two-year field study. Chemosphere. 364(143068), pp. 1-
    https://doi.org/10.1016/j.chemosphere.2024.143068 (Journal)
  26. Page, A. L. (Ed.). 1982. Methods of soil analysis. Part 2. Chemical and microbiological properties (2nd ed.). ASA and SSSA. https://doi.org/10.2134/agronmonogr9.2.2ed (Journal)
  27. Ruiz-Lozano, Aroca, J.M., Zamarre˜no R. and Metal ´ A. 2016. Arbuscular mycorrhizal symbiosis induces strigolactone biosynthesis under drought and improves drought tolerance in lettuce and tomato. Plant Cell Environment. 39, 441–452. https://doi.org/ 10.1111/pce.12631 (Journal)
  28. Saeed, F., Afzaal, M., Niaz, B., Arshad, M.U., Tufail, T., Hussain, M.B. and Javed, A. 2018. Bitter melon (Momordica charantia): a natural healthy vegetable. International Journal of Food Properties. 21 (1), 1270–1290. https://doi.org/10.1080/10942912.2018.1446023 (Journal)
  29. Singh, S., Parihar, P., Singh, R., Singh, V.P. and Prasad, S.M. 2024. Arbuscular mycorrhizal fungi mediated modulation of physiological and biochemical responses in plants under drought stress: A comprehensive review. Journal of Plant Growth Regulation, 43(2), 521–538. DOI: 10.1007/s00344-023-11150-7 (Journal)
  30. Smith, S. E. and Read, D. J. 2010. Mycorrhizal symbiosis (3rd ed.). Academic Press. https://doi.org/10.1016/B978-0-12-370526-6.00001-1 (Journal)
  31. Smith, S. E., Facelli, E., Pope, S., & Smith, F. A. (2010). Plant performance in stressful environments: interpreting new and established knowledge of the roles of arbuscular mycorrhizas. Plant and Soil, 326, 3–20. https://doi.org/10.1007/s11104-009-9981-5 (Journal)
  32. Wang, X., Pan, Q., Chen, F., Yan, X. and Liao, H. 2023. AMF-enhanced drought tolerance in soybean: physiological and molecular mechanisms. Frontiers in Plant Science, 14, 1198324. https://doi.org/10.3389/fpls.2023.1198324 (Journal)

Zulfiqar, F., Ashraf, M. and Ahmad, P. 2021. Osmolytes and antioxidant defense in plants under drought stress. Plant Physiology and Biochemistry, 162, 63–73. https://doi.org/10.1016/j.plaphy.2021.02.020 (Journal)

علوم و تحقیقات بذر ایران
دوره 12، شماره 4
اسفند 1404
صفحه 61-75

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