تأثیر پرایمینگ با آسکوربیک‌ اسید بر برخی ویژگیهای فیزیولوژیک، بیوشیمیایی و جوانه‌زنی بذر مرزه تابستانه (Satureja hortensis L.) تحت تنش خشکی

نوع مقاله : مقاله پژوهشی

نویسنده

گروه زراعت، واحد کلیبر، دانشگاه آزاد اسلامی، کلیبر، ایران.

10.22124/jms.2025.9441

چکیده

به‌منظور بررسی اثر آسکوربیک‌ اسید بر برخی ویژگی‌های فیزیولوژیک، بیوشیمیایی و جوانه‌زنی بذر مرزه تابستانه تحت تنش خشکی، آزمایشی به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار در آزمایشگاه فیزیولوژی دانشگاه آزاد اسلامی واحد کلیبر در سال زراعی 1403 اجرا گردید. تیمارهای آزمایش شامل غلظت‌های مختلف اسید آسکوربیک در چهار سطح (0، 5/0، 1 و 2 میلی‌مولار) و سطوح مختلف تنش خشکی ایجاد شده با پلی‌اتیلن گلایکول 6000 در پنج سطح با پتانسیل‌های (0 (آب مقطر) ، 4-، 8-، 12- و 16- مگاپاسکال) بودند. نتایج نشان داد که اثر تنش خشکی و غلظت‌های مختلف اسید آسکوربیک بر مؤلفه‌های جوانه‌زنی، رنگیزه‌های فتوسنتزی و آنزیم‌های آنتی‌اکسیدان در سطح احتمال یک درصد معنی‌دار بود. برهمکنش اسید آسکوربیک و تنش خشکی بر طول ریشه‌چه و ساقه‌چه، میزان کلروفیل a، کلروفیل کل و میزان فعالیت آنزیم‌های سوپراکسید دیسموتاز و آسکوربات پراکسیداز معنی‌دار بود. نتایج نشان داد که بیش‌ترین میزان کلروفیل a (15/6 میلی‌گرم در گرم وزن تر) و کلروفیل کل (87/10 میلی‌گرم در گرم وزن تر) مربوط به پیش‌‌تیمار اسید آسکوربیک با غلظت 2 میلی‌مولار تحت شرایط بدون اعمال تنش (شاهد) و بیش‌ترین میزان فعالیت آنزیم سوپراکسید دیسموتاز (52/472 واحد بر میلی‌گرم پروتئین) و آسکوربات پر‌اکسیداز (25/0 واحد بر میلی‌گرم پروتئین) مربوط به پیش‌تیمار اسید آسکوربیک با غلظت 2 میلی‌مولار تحت شرایط تنش 16- مگاپاسکال بود. به‌طور کلی نتایج حاصل از  این پژوهش نشان داد که استفاده از محلول‌پاشی اسید آسکوربیک به‌ویژه در غلظت‌ 2 میلی‌مولار موجب کاهش اثرات منفی تنش‌ کم‌آبی و هم‌چنین بهبود شاخص‌های جوانه‌رنی، رنگیزه‌های فتوسنتزی و افزایش در فعالیت آنزیم‌های آنتی‌اکسیدان در بذر مرزه شد.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of priming with ascorbic acid on some physiological, biochemical and germination characteristics of summer savory (Satureja hortensis L.) seeds under drought stress

نویسنده [English]

  • Ahmad Afkari
Department of Agronomy, Kaleibar Branch, Islamic Azad University, Kaleibar, Iran
چکیده [English]

In order to investigate the effect of ascorbic acid on some physiological, biochemical and seed germination characteristics of summer savory under drought stress, a factorial experiment was conducted in a completely randomized design with three replications in the physiology laboratory of Islamic Azad University, Kalibar Branch, in the crop year 2024. The experimental treatments included different concentrations of ascorbic acid at four levels (0, 0.5, 1 and 2 mM) and different levels of drought stress created with polyethylene glycol 6000 at five levels with potentials of zero (distilled water), -4, -8, -12 and -16 MPa. The results showed that the effect of drought stress and different concentrations of ascorbic acid on germination components, photosynthetic pigments and antioxidant enzymes was significant at the 1% probability level. The interaction of ascorbic acid and drought stress on root and shoot length, chlorophyll a content, total chlorophyll and the activity of superoxide dismutase and ascorbate peroxidase enzymes was significant. The results of comparing the average interaction of drought stress and different concentrations of ascorbic acid showed that the highest levels of chlorophyll a (6.15 mg/g/fw) and total chlorophyll (10.87 mg/g/fw) related to priming with ascorbic acid at a concentration of 2 mM under conditions without stress (control) and pretreatment with ascorbic acid at a concentration of 2 mM, and the highest levels of superoxide dismutase enzyme activity (472.52 u/mg.protein) and ascorbate peroxidase (0.25 u/mg.protein) it was related to priming with ascorbic acid at a concentration of 2 mM under stress conditions of -16 MPa. In general, the results of this study showed that the use of foliar spraying of ascorbic acid, especially at a concentration of 2 mM, reduced the negative effects of water deficit stress and also improved germination indices, photosynthetic pigments, and increased antioxidant enzyme activity in savory seeds.

کلیدواژه‌ها [English]

  • Antioxidant enzymes
  • Germination
  • Osmotic potential
  • Photosynthetic pigments
  • Priming
  • Savory
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