ارزیابی اثر پرایمینگ بذر با اسید سالیسیلیک بر تحمل تنش شوری در مرحله جوانه‌زنی و رشد گیاهچه گوجه‌فرنگی (Solanum lycopersicum) رقم هیبرید بریویو

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی گیاهان زراعی، گروه تولیدات گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه گنبد کاووس، گنبد کاووس، ایران.

2 دانشگاه گنبد کاووس

3 دکتری اکولوژی گیاهان زراعی و پژوهشگر پسادکتری بنیاد علم ایران. تهران، ایران.

4 استادیار، گروه تولیدات گیاهی، دانشکده کشاورزی و منابع طبیعی دانشگاه گنبد کاووس، گنبد کاووس، ایران.

10.22124/jms.2025.9428

چکیده

مرحله جوانه‌زنی نقش کلیدی در رشد و استقرار اولیه گیاه دارد و تکنیک پرایمینگ بذر با ایجاد تغییرات فیزیولوژیکی و بیوشیمیایی، بذر را پیش از قرارگیری در بستر کاشت برای جوانه‌زنی مؤثر . این پژوهش در سال 1404 با هدف بررسی تأثیر پرایمینگ بذر گوجه‌فرنگی رقم هیبرید بریویو با اسید سالیسیلیک بر شاخص‌های جوانه­زنی و پارامترهای رشدی گوجه فرنگی تحت تنش شوری، در دو آزمایش مجزا (آزمایشگاه و گلخانه) به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار در دانشکده کشاورزی و منابع طبیعی دانشگاه گنبد کاووس اجرا گردید. فاکتورهای آزمایش شامل اسید سالیسیلیک در چهار سطح (0، 2، 5/2 و 3 میلی­گرم بر لیتر) و شوری در پنج سطح (0، 30 ، 60 ، 90 و 120 میلی­مولار کلرید سدیم) بودند. داد که پرایمینگ بذر با اسید سالیسیلیک در شرایط تنش شوری موجب بهبود صفات جوانه‌زنی و رشد گیاهچه گوجه‌فرنگی از جمله درصد و سرعت جوانه‌زنی، طول گیاهچه، وزن خشک، شاخص بنیه بذر و همچنین محتوای کلروفیل‌های a، b و کل گردید. به‌طوری که در مجموع می‌توان نتیجه گرفت که پرایمینگ بذر گوجه‌فرنگی با اسید سالیسیلیک، به‌عنوان یک رویکرد مقرون‌به‌صرفه، قابل دسترس و کاربردی، می‌تواند تحمل گیاه به تنش شوری را تقویت کرده و به پایداری تولید این محصول در مناطق با خاک شورکه حدود ۲۵ درصد از اراضی ایران را شامل می‌شود، کمک نماید.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of seed priming with salycilic acid on salt stress tolerance during germination and seedling growth stages of tomato (Solanum lycopersicum L. cv. Brivio hybrid)

نویسندگان [English]

  • Reza Rezvani 1
  • Abbas Biabani 2
  • Zahra Taghizadeh Tabari 3
  • Masoumeh Naeemi 4
1 Ph.D. Student of Crop Physiology Department of Plant Production, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Iran.
2 University of Gonbad Kavoos
3 Ph.D. in Crop Ecology and Postdoctoral Researcher, Iran National Science Foundation (INSF), Tehran, Iran.
4 Assistant Professor, Department of Plant Production, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Iran.
چکیده [English]

The germination is crucial for the early growth and establishment of the plant and the seed priming technique, by inducing physiological and biochemical changes, prepares seed for effective germination prior to placement the planting medium. This study, conducted in 2025, aimed to assess the influence of salycilic acid pretreatment on the germination indices and growth parameters of tomato (Solanum lycopersicum L.) under salt stress. Two experiments, one in the laboratory and the other in a greenhouse, were performed using a factorial design based on a completely randomized design with three replications at the Faculty of Agriculture and Natural Resources, Gonbad Kavous University. The experimental factors included salicylic acid priming at four concentrations (0, 2, 2.5, and 3 mg/l-1) and salinity at five levels (0, 30, 60, 90, and 120 mM NaCl). The results of a 14-day germination test under laboratory conditions indicated that seed priming with salicylic acid under salt stress conditions, in comparison to the control, resulted in enhancements in germination and growth traits of tomato plants, including germination percentage and rate, seedling length, dry weight, seed vigor index, and the contents of chlorophyll a, b and total chlorophyll. The highest germination rate (8.22 seeds per day) and seedling vigor index (9.80) were observed in the treatment with 3 mg/l-1 salicylic acid under without saline conditions. In the greenhouse experiment, application of salicylic acid for five weeks after sowing tomato seeds in seedling trays enhanced growth traits, including plant dry weight and chlorophyll a, b, and total chlorophyll content. The interaction between salicylic acid and salinity indicated that the application of this compound, particularly at concentrations of 2.5 and 3 mg/l-1, mitigated the negative effects of salt stress on total chlorophyll content, such that no significant changes were observed at salinity levels up to 60 mM NaCl. In conclusion priming tomato seeds with salicylic acid, as a cost-effective, accessible, and practical approach, can enhance plant tolerance to salt stress and contribute to the sustainability of tomato production in saline soils, which account for approximately 25% of Iran's arable lands.
 

کلیدواژه‌ها [English]

  • Culture medium
  • Chlorophyll
  • Germination percentage and rate
  • Pretreatment
  • Seedling vigor index
  • Vegetables
  • vegetative traits

مراجع

Abdulbaki, A. A. and Anderson, J. D. 1973. Vigor determination in soybean by multiple criteria. Journal of Crop Sciences, 13: 630-633. https://doi.org/10.2135/cropsci1973 (In Persian) (Journal)
Agah, F. and Nabavi Kalat, S. M. 2013. Study on the effects of priming on improving the germination indices of lentil (Lens culinaris Melik.) seeds under salt stress. Journal of Seed Science and Technology, 3(2): 53-61. (In Persian with English Summery) (Journal)
Ahmad, F., Kamal, A., Singh, A., Ashfaque, F., Alamri, S., and Siddiqui, M. H. 2020. Salicylic acid modulates antioxidant system, defense metabolites, and expression of salt transporter genes in Pisum sativum under salinity stress. Journal of Plant Growth Regulation, 41(4): 1905– 1918. https://doi.org/10.1007/s00344-020-10271-5 (Journal)
Ahn, J. and Chung, I. 2000. Allelopathic potential of rice hull on germination and seedling growth of barnyardgrass. journal of Agronomy. 92(6): 1162-1167. https://doi.org/10.2134/agronj2000.9261162x (Journal)
Arnon, A. N. 1967. Method of extraction of chlorophyll in the plants. Agronomy. 23: 112-121. (Journal)
Azarmi, R., Tabatabaei, J. and Chaparzadeh, N. 2018. Effects of Mg2+ and root zone temperature on growth, yield and physiological properties of greenhouse cucumber (Cucumis sativus L.) in hydroponics. Journal of Soil and Plant Interactions Isfahan University of Technology, 9(2): 11-22. https://doi.org/10.29252/ejgcst.9.2.11 (In Persian) (Journal)
Abdoli, A. Ramezani Moghadam, J., Hosseini, Y., Nikpour, M. R. and Dehghan, H. 2021. The Effect of Salinity Stress on Tomato Root Characteristics (Saint Pierre) and its Yield under Irrigation Time Management. Journal of Horticultural Science, 35(2): 233-234. (In Persian) (Journal)
Barwal, S. K., Goutam, C., Chauhan, C., Vimala, Y., Alyemeni, M. N., Ahmad, P., and Siddique, K. H. M. 2023. Salicylic acid alleviates salt-induced phytotoxicity by modulating physiochemical attributes and upregulating the AsA-GSH cycle and glyoxalase system in Capsicum annuum L.. South African Journal of Botany, 161, 222–237. https://doi.org/10.1016/j.sajb.2023.07.061 (Journal)
Chen, S., Zhao, C. B., Ren, R. M., and Jiang, J. H. (2023). Salicylic acid has the potential to enhance tolerance in horticultural crops against abiotic stress. Frontiers in Plant Science, 14: Article 1141918. https://doi.org/10.3389/fpls.2023.1141918 (Journal)
Darafshi, H. R., Arvin, S. M. J. and Nejadalimoradi, F. 2025. Investigating the priming with salicylic acid on seed germination, seedling growth, and fruit quality of tomato (Solanum lycopersicum L. cv. Tina) in the field. Iranian Journal of Seed Science and Technology, 14(2): 1-13. https://doi.org/10.22092/ijsst.2024.364903.1515
Delian, E. N. A., Badulescu, L., Dobrescu, A., chira, L. and Lagunovschi-Luchian, V. 2017. A brief overview of seed priming benefits in tomato. Journal of Romanian Biotechnological Letters, 22(3): 12505-12513. (Journal)
Egert, M., and Tevini, M. 2002. Influence of drought on some physiological parameters symptomatic for oxidative stress in leaves of chives (Allium schoenoprasum). Journal of Environmental and Experimental Botany: 48(1): 43–49. https://doi.org/10.1016/S0098-8472(02)00008-4 (Journal)
Foolad, M. R., Subbiah, P., Kramer, C., Hargrave, G. and Lin, G. Y. 2003. Genetic relationship among cold, salt and drought tolerance during seed germination in an interspecific cross of tomato. Journal of Euphytica, 130:199-206. (Journal)
Ghasemi Arian, A., Ghorbani, R., Nasripour Yazdi, M. and Mesdaghi, M. 2016. Effect of temperature on seed germination characteristics of Dorema ammoniacum. Iranian Journal of Biology, 29(3): 686-693. https://doi.org/20.1001.1.23832592.1395.29.3.20.6 (In Persian) (Journal)
Hampson, C.  R., and Simposon, D. M. 1990. Effect of temperature, salt and osmotic potential on early growth of wheat. П. Early seedling growth. Canadian Journal of Botany, 68(1): 524-528. https://doi.org/10.1139/b90-073 (Journal)
Hafeznia, M., Mashayekhi, K., Ghaderifar, F. and Mousavizadeh, S. J. 2014. The effect of salicylic acid spraying time on some pigment content and morphological traits of tomato fruit. Journal of Plant Production, 22(2): 203–217 (In Persian) (Journal)
Hochmuth, G. J. and Hochmuth, R. C. 2013. Production of greenhouse Tomatoes-Florida greenhouse vegetable production handbook. University of Florida. Institute of Food and Agricultural Sciences. Corporate contributor: Florida Cooperative Extension. (Book)
Hunter, E. A., Glasbey, C. A. and Naylor, R. E. L. 1984. The analysis of data from germination tests. The Journal of Agricultural Science. 102: 207-213. (Journal)
ISTA (International Seed Testing Association), 2025. International Rules for Seed Testing. International Seed Testing Association, Bassersdorf, Switzerland.
Ikic, I., Maricevic, M., Tomasovic, S., Gunjaca, J., Sarcevic, Z. and Arcevic, H. 2012. The effect of germination temperature on seed dormancy in creation grown winter wheats. Journal of Euphytica, 188: 25-34. https://doi.org/10.1007/s10681-012-0735-8 (Journal)
Javedanipour, E. and Feizi, H. 2022. Role of Spermidine and γ-Aminobutyric Acid and Hydro priming on alleviation of salinity effect in germination and growth parameters of cucumber (Cucumis sativus L. cv. Beit Alpha) seed. Iranian Journal of Seed Science and Technology, 10(4): 103-118. https://doi.org/10.22092/ijsst.2020.351728.1362 (In Persian) (Journal)
Karami, L., Hedayat, M. and Farahbakhsh, S. 2020. Effect of salicylic acid priming on seed germination and morphophysiological and biochemical characteristics of tomato seedling (Lycopersicom esculentun). Journal of Seed Research, 7(1): 165-179. https://doi.org/10.29252/yujs.7.1.165 (In Persian) (Journal)
Kim, Y. H, Yang, H. C., Bae, Y. H., Hyeon, S. J. Hwang, S. J., Kim, D. H. and Jang, D. C. 2023. Preventing overgrowth of cucumber and tomato seedlings using difference between day and night temperature in a plant factory with artificial lighting. Journal of Plant, 12(17): 3164.   https://doi.org/10.3390/plants12173164 (Journal)
Mady, E., Abd El‑Wahed, A. H. M., Awad, A. H., Asar, T. O., Al‑Farga, A., Abd El‑Raouf, H. S., Randhir, R., Alnuzaili, E. S., El‑Taher, A. M., and Randhir, T. O. 2023. Evaluation of salicylic acid effects on growth, biochemical, yield, and anatomical characteristics of eggplant (Solanum melongena L.) plants under salt stress conditions. Agronomy, 13: 2213. https://doi.org/10.3390/agronomy13092213 (Journal)
Maguire, J. D. 1962. Speed of germination aid in selection and evaluation for seedling emergence and vigor. Journal of Crop Science, 2(2): 176–177. https://doi.org/10.2135/cropsci1962.0011183X000200020033x (Journal)
Miller, T. R. and Chapman, S. R. 1978. Germination responses of three forage grasses to different concentration of six salts. Journal of Range Management, 31(2): 123-124. https://doi.org/10.2307/3897659 (Journal)
Mohammadi, M., Liaghat, A. and Molavi, H. 2010. Optimization of water use and determination of tomato sensitivity coefficients under combined salinity and drought stress in Karaj. Journal of Water and Soil, 24(3): 583-593. https://doi.org/10.22067/jsw.v0i0.3629 (In Persian) (Journal)
Rezvani, R., Kamkar, B. and Jabbari Badkhor, Z. 2025. Investigating the role of priming with humic acid on modulating the effect of salinity stress on the germination and growth indices of cucumber (Cucumis sativus L. cv. Saba hybrid) seed. Iranian Journal of Seed Science and Technology, 14(3): 43-60. https://doi.org/10.22092/ijsst.2024.364534.1511 (In Persian) (Journal)
Sakhabutdinova, A. R., Fatkhutdinova, D. R., Bezrukova, M. V. and Shakirova, F. M. 2003. Salicylic acid prevents the damaging action of stress factors on wheat plants. Bulgarian Journal of Plant Physiology, 21: 314-319. (Journal)
Salehzade H., Izadkhah Shishvan, M. and Chiyasi, M. 2009. Effect of seed priming on germination and seedling growth of wheat (Triticum aestivum L.). Journal of Biological Sciences, 4(5): 629-631.  https://doi.org/rjbsci.2009.629.631 (In Persian) (Journal)
Shefazadeh Shahrebabki, M. K., Hosseinifarahi, M., Mohamadineia, G. 2020. Improvement of Salt Tolerance and Nutrient Absorption in Pepper (Capsicum annum) through application of salicylic acid and humic acid. Journal of Horticultural Science, 34(1): 91-106. (In Persian) (Journal)
Shumye Adilu, G and Gedamu Gebre, Y. 2021. Effect of salinity on seed germination of some tomato (Lycopersicon esculentum Mill.) varieties. Journal of Aridland Agriculture, 7: 76-82. https://doi.org/10.25081/jaa.2021.v7.6588 (Journal)
Siahmansour, S., Ehteshamnia, A. and Rezaeinejad, A. 2020. Effect of salicylic acid foliar application on Morpho- physiological and biochemical traits of Goldenberry (Physalis peruviana L.) under salinity stress condition. Journal of Plant production research, 27(1): 167-178. https://doi.org/10.22069/jopp.2020.16087.2448 (In Persian) (Journal)
Taheri, S., Barzeghar, T. and Zoeam zadeh, F. 2017. Effect of salicylic acid pre-treatment on cucumber and watermelon seeds germination under salinity stress. Iranian Journal of Seed Science and Research, 3(4): 15-27. (In Persian) (Journal)
Vijay, C. R., Thriveni, M. C. and Shivamurthy, G. R. 2012. Effect of growth regulators on seed germination and its significance in the management of Aeginetia indica L. A root holoparasite. American Journal of Plant Sciences, 3: 1490-1494. https://doi.org/10.4236/ajps.2012.310179 (Journal)
Zamani, S., Ghasemnezhad, A., Alizadeh, M. and Alami, M. 2018. Effect of salinity and salicylic acid on morphological and photosynthetic pigments changes of callus of artichoke (Cynara scolymus L.). Journal of Crop Breeding, 10(26): 128-138. https://doi.org/10.29252/jcb.10.26.128 (In Persian) (Journal)
Zhang, M., Wang, Z., Yuan, L., Yin, C., Chang, G., Wang, L. and Zhang, H. 2012. Osmopriming improves tomato seed vigor under aging and salinity stress. African Journal of Biotechnology, 11(23): 6305-6311. https://doi.org/10.5897/AJB11.3740 (Journal)
علوم و تحقیقات بذر ایران
دوره 12، شماره 3
مهر 1404
صفحه 66-80

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