اثر پرایمینگ و فرسودگی بذر بر شاخص‌های جوانه‌زنی و فعالیت برخی آنزیم‌های هیدرولیتیک و چرخه گلی‌اکسیلات در ذرت (Zea mays L.)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دانشگاه محقق اردبیلی

2 گروه زراعت و اصلاح نباتات، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی

10.22124/jms.2023.23130.1726

چکیده

به‌منظور بررسی اثر پرایمینگ و فرسودگی بذر بر شاخص‌های جوانه‌زنی و فعالیت برخی آنزیم‌های هیدرولیتیک و چرخه گلی‌اکسیلات در گیاهچه ذرت آزمایشی به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار در دانشگاه محقق اردبیلی در سال 1401 اجرا گردید. تیمارهای آزمایش شامل تیمار پیری تسریع شده در سه سطح (صفر، 11 و 13روز) و پرایمینگ در چهار سطح (شاهد، هیدرو پرایمینگ، پرایمینگ با اسید سالیسیلیک (100 میلی‌مولار) و جیبرلین (20 میلی‌مولار)) بود. نتایج نشان داد که فرسودگی شاخص‌های جوانه‌زنی شامل درصدجوانه‌زنی، سرعت جوانه‌زنی، میانگین جوانه‌زنی روزانه، ضریب جوانه‌زنی و ارزش جوانه‌زنی را کاهش داد، ولی پرایمینگ با اسیدسالیسیلیک، هیدرو به‌ویژه جیبرلین این صفات را بهبود بخشید. کمترین سرعت جوانه‌زنی روزانه و میانگین مدت جوانه‌زنی در هیدروپرایمینگ، پرایمینگ با اسیدسالیسیلیک مخصوصا پرایمینگ با جیبرلین و بدون فرسودگی حاصل شد. فعالیت آنزیم‌های آلفا آمیلاز و مالات سنتاز در تیمار جیبرلین و بدون فرسودگی نسبت به شاهد به ترتیب با 71 درصد و 26 درصد افزایش نشان دادند. بـیش‌تـرین فعالیت آنزیم لیپاز در تیمار جیبرلین (41/73 واحد بر میلی‌گرم پروتئین) حاصل گردید. همچنین، فرسودگی باعث کاهش فعالیت آنزیم لیپاز شد. درکل، استفاده از پیش‌تیمار جیبرلین موجب تقویت شاخص‌های جوانه‌زنی و فعالیت برخی آنزیم‌های هیدرولیتیک و چرخه گلی‌اکسیلات بذرهای فرسوده ذرت شد و رشد گیاهچه را افزایش داد.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of seed priming and aging on germination indices and activity of some hydrolytic enzymes and glyoxylate cycle in corn (Zea mays L.)

نویسندگان [English]

  • haniyeh saadat 1
  • Mohammad Sedghi 2
1 Student of Mohaghegh Ardabili University
2 Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Iran
چکیده [English]

In order to investigate the effect of priming and aging on germination indices and activity of hydrolytic enzymes and cycle glyoxylate in corn seed, a factorial experiment was conducted based on completely randomized design at the University of Mohaghegh Ardabili in 2022 with 3 replications. Treatments were accelerated aging (control without aging, 11 and 13 days) and priming (control, hydro-priming, priming by gibberellin (20 mg lit-1) and salicylic acid (100 mg lit-1)).The results showed that aging of germination indicators including germination percentage (GR), Germination Rate (GR), Mean Daily Germination (MDG), Germination Coefficient (GC), Germination Value (GV) decreased, but priming with salicylic acid, hydro especially gibberellin improved these traits. The lowest Daily Germination Speed (DGS) and Mean Germination Time (MGT) were obtained in hydropriming, priming with salicylic acid, especially priming with gibberellin and the control (without priming). The amylase and malate synthase enzymes activity in gibberellin treatment and non-aged compared to the control showed an increase respectively about 71% and 26%. The most lipase enzyme activity (73.41 unit mg-1 protein) was observed in treatment with gibberellin. Aging decreased lipase enzyme activity. In general, using gibberellin pretreatment strengthened weak corn seeds the germination indices and activity of hydrolytic enzymes glyoxylate cycle and increased seedling growth.

کلیدواژه‌ها [English]

  • Aging
  • Amylase
  • Germination Indicators
  • Lipase
  • Malate Synthase
  • Priming
Abdoli, M. 2020. Effect of aging of seed and hydro-priming on germination characteristics and activity of some antioxidant enzymes of hybrid corn (Zea mays L.). Iranian Journal of Seed Science and Research, 7(2): 147-159. (In Persian)(Journal)
Afzal, I., Aslam, N., Mahmood, F., Hameed, A., Irfan, S. and Ahmad, G. 2004. Enhancement of germination and emergence of canola seeds by different priming techniques. Biological, Santa Cruz do Su. 16(1): 19-34. (Journal)
Aghighi Shahverdi, M., Omidi, H. and Mousavi, S. A. 2017. Effect of Chitosan on Seed Germination and Biochemical Traits of Milk Thistle (Silybum marianum L.) Seedling under Salt Stress. Iranian Journal of Seed Research, 3(2): 118-105. (In Persian)(Journal)
Amrian, M. 2015. The effect of different levels of selenium nanoparticles on onion (Allium cepa L.) seed germination. The 5th National Nanotechnology Conference from Theory to Application. 20 Feb, Isfahan, Iran. (Conference)
Ansari, A. and Sharifzadeh, F. 2013. Improving germination characteristics of mountain rye (Secale montanum) primed seeds under slow moisture reduction and accelerated ageing conditions. Journal of Seed Science and Technology, 2(2): 76-68. (In Persian)(Journal)
Armin, M., Asgharipour, M. and Rezvani-Omrani, M. 2010. The effect of seed priming on germination and seedling growth of watermelon (Citrullus lanatus). Advences in Enviremental Biology, 4(3): 501-505. (Journal)
Asadi Aqbalaghi, M., Permon, Q. and Mansani, H. 2015. The effect of accelerated aging on the process of seed germination and seedling growth of pumpkin. Journal of Seed Research, 5(2): 60-68. (In Persian)(Journal)
Ashraf, M., Athar, H. R., Harris, P. J. C. and Kwon, T. R. 2008. Some prospective strategies for improving crop salt tolerance. Advanced in Agronomy, 97: 45-92. (Journal)
Babaei, K., Tajbakhsh, M. and Siosemardeh, A. 2018. Effect of Priming and Sowing Date of Seed on Growth Indices of Plant and yield and Yield Components of seed of Maize Single Cross 260 (Fajr). Plant Production Technology. 19(2): 209-193. (In Persian)(Journal)
Bagheri, M., Amoozegar, M. A., Schumann, P., Didari, M., Mehrshad, M., Sproer, C., Sanchez-Porro, C. and Ventosa, A. 2013. Ornithinibacillus halophilus sp. nov., a moderately halophilic, Gram-stain-positive, endospore-forming bacterium from hypersaline lake. International Journal of Systematic and Evolutionary Microbiology, 63: 844-848. (Journal)
Baharvand, N., Mahdavi, B. and Dehajipour Heidarabadi, M. 2017. Effect of ascorbic acid on germination and activities of antioxidant enzymes of deteriorated safflower Goldasht cultivar seeds. Iranian Journal of Seed Science and Research, 4(3): 1-12. (In Persian)(Journal)
Bailly, C., Benamar, A., Corbineau, F. and Come, D. 2000. Antioxidant systems in sunflower (Helianthus annuus L.) seeds as affected by priming. Seed Science Research, 10: 35–42. (Journal)
Berg, J. M., Tymoczko, J. L. and Stryer, L. 2002.Biochemistry (6th ed.). W. H. Freeman Publishing, New York. (Book)
Bose, B. and Mishra, T. 1992. Response of wheat seed to presowing seed treatment with Mg (NO3) 2. Journal of Annals of agricultural science, 13: 132-136. (Journal)
Caruso, G., C. Cavaliere, P. Foglia, R. Gubbiotti, R. Samperi and A. Lagana, 2009. Analysis of drought responsive proteins in wheat (Triticum durum) by 2D-PAGE and MALDI-TOF mass spectrometry. Plant Science, 177: 570–576. (Journal)
Chojnowski, M., Corbineau, F. and Come, D. 1997. Physiological and biochemical changes induced in sunflower seeds by osmopriming and subsequent drying, storage and aging. Seed Science Research, 7: 323-332. (Journal)
Cooper, T. G. D. and Beevers, H. 1969. Mitochondria and glyoxysomes from castor bean endosperm: enzyme constituents and catalytic capacity. Journal of Biological Chemistry, 244: 3507–3513. (Journal)
Darabi, F., Valipour, M., Naseri, R. and Moradi, M. 2017. The Effects of Accelerated Aging Test on Germination and Activity of Antioxidant Enzymes of Maize (Zea mays) Hybrid Varieties Seeds. Iranian Journal of Seed Research, 4(1): 45-59. (In Persian)(Journal)
Duman, I. 2006. Effect of seed priming with PEG and K3PO4 on germination and seedling growth in lettuce. Pakistan Journal of Biological Sciences, 9(5): 923-928. (Journal)
Eastmond, P. J. and Graham, L. A. 2001. Re-examining the role of the glyoxylate cycle in oilseeds. Trends in Plant Science, 6(2): 72-77. (Journal)
Ellis, R. H., Roberts, E. H. 1980. Seed physiology and seed quality in soybean. Advances in Legume Science. pp: 287-311. (Journal)
Ensign, S. A. 2006. Revisiting the glyoxylate cycle: alternate pathways for microbial acetate assimilation. Molecular Microbiology, 61(2): 274-276. (Journal)
Esterbauer, H. and Grill, D. 1978. Seasonal variation of glutathione and glutathione reductase in needles of Picea abies. Plant Physiology. 61:119–121. (Journal)
Fathi Amirkhiz, K., Amini Dehaghi, M.and Hashmati, S. 2014. Investigating the effect of iron fertilization on chlorophyll content, quantum efficiency of photosynthesis II and some biochemical traits in safflower under low irrigation conditions. Iranian Journal of Agricultural Plant Sciences, 1: 137-145. (In Persian)(Journal)
Ghana, S. G. and Schillinger, W. F. 2003. Seed priming winter wheat for germination, emergence, and yield. Crop Science, 43(6): 2135-2141. (Journal)
Ghasemi Golazani, K. and Dalil, B. 2011. Germination tests and seed strength. Publications University of Mashhad. (In Persian)(Book)
Gholami, SH. and Dehaghi, M. A. 2022. The effect of priming with different concentrations of selenium on germination indices of quinoa seedlings (Chenopodium quinoa Willd.). Agronomy, 24(1): 85-95. (In Persian)(Journal)
Hajiabbasi, M., Tavakkol Afshari, R., Abbasi, A. and Kamaei, R. 2021. Effects of salicylic acid and ethylene on the germination and gene expression of alpha and beta amylase in deteriorated soybean seeds (Glycine max). Iranians Journal of Seed Science and Technology, 10(1): 156-141. (In Persian)(Journal)
Hampton, J. G. 2003. Methods of viability and vigour testing: A critical and appraisal. In: Basra, A. (Eds.) Seed Quality, Basic Mechanisms and Agricultural Implications. CBS Publishers and Distributers, New Delhi. pp: 81 -118.
Hartman, H., Kester, D. and Davis, F. 1990. Plant Propagation, Principle and Practices (9th ed.). Pearsom, Kindle. (Book)
Huang, A. H. C. 1985. Lipid bodies, 1.  In: Linskins, H. F. and Jackson, F. (Eds.) Modern Methods of Plant Analysis, Berlin. pp: 145-151.
Hunter, E., Glasbey, C. and Naylor, R. 1984. The analysis of data from germination tests. The Journal of Agricultural Science, 102(1): 207-213. (Journal)
Hurly, R. F., Van Staden, J. and Smith, M. T. 1991. Improved germination in seeds of guayule (Parthenium argentatum) following polyethylene glycol and gibberellic acid pretreatments. Annals of Applied Biology, 118: 175-184. (Journal)
Hussein, H. J. 2017. The Effect of Seed Priming with Salicylic Acid and Ascorbic Acid on Viability of Local Okra (Abelmoschus Esculentus L.) Seeds Stored for Three Years. Journal of Global Pharma Technology, 8(9): 110-115. (Journal)
ISTA. 2012. International Rules for Seed Testing. Bassersdorf, Switzerland: The
International Seed Testing Association (ISTA). (Handbook)
Job, D., Whalley, C. and Johnstone, S. M. L. 2005. Grey matter changes over time in high risk subjects developing schizophrenia. Neuroimage, 25(4): 1023-1030. (Journal)
Jyoti, U. and Malik, C. P. 2013. Seed deterioration: A review. International Journal of Life Science Botany and Pharmacy Research, 2: 374-85. (Journal)
Kapoor, R., Arya, A., Siddiqui, M. A., Amir, A. and Kumar, H. 2010. Seed deterioration in Chickpea (Cicer arietinum L.) under accelerated aging. Asian Journal Plant Science, 9(3): 158- 162. (Journal)
Khajeh Hosseini, A., Powell, A. and Bingham, I. J. 2003. The interaction between salinity stress and seed vigour during germination of soyabean seeds. Seed Science and Technology, 31: 715-725. (Journal)
Khatami, S. R., Sedghi, M. and Seyed Sharifi, R. 2019. Effect of priming and osmotic stress on the germination and activity of hydrolytic and glyoxylate cycle enzymes of hybrid maize (Zea mays L. SC704) seed. Iranian Journal of Seed Science and Research, 6(1): 67-78. (In Persian)(Journal)
Khushvagti, H. 2019. Investigating the effect of seed priming on the morphological traits of corn seeds. The 4th International Congress of Agricultural Development, Natural Resources. 13-15 Feb, Environment and Tourism of Iran, Tabriz, Iran. (Conference)
Liopa-Tsakalidi, A., Kaspiris, G., Salahas, G. and Barouchas, P. 2012. Effect of salicylic acid (SA) and gibberellic acid (GA1) pre-soaking on seed germination of Stevia (Stevia rebaudiana) under salt stress. Journal of Medicinal Plants Research, 6: 416-423. (Journal)
McDonald, M. B. 1999. Seed deterioration: physiology, repair and assessment. Seed Science Technology, 27: 177-237. (Journal)
Migahid, M. M., Elghobashy, R. M., Bidak, L. M. and Amin. A. W. 2019. Priming of Silybum marianum (L.) Gaertn seeds with H2O2 and magnetic field ameliorates seawater stress. Heliyon, 5(6): e01886.
Mohamadian, E., Kianmehr, H., Ataei Somagh, H., Azad Nafas Mahjor, N., Safari, F. and Safarzadeh, A. 2018. Effect of methyl jasmonate pretreatment on germination indices and biochemical traits of Stevia seedlings (Stevia rebuadiana) under salt stress. Iranian Journal of Seed Research, 5(1): 101-117. (In Persian)(Journal)
Moradi, A., Sharifzadeh, F., Tawak Afshari, R. and Maali Amiri, R. 2010. Effect
of seed priming on germination and growth of Agropyron elongatum at normal and
drought stress condition. Journal of Range, 4(3): 362-373. (In Persian)(Journal)
Murray, S. and Eisvand, H. R. 2018. The effect of priming with salicylic acid and ascorbic acid on germination indices and biochemical traits in wheat seed deterioration. Iranian Seed Science and Research, 6(3): 398-381. (In Persian)(Journal)
Namdari, A. and Sharifzade, F. 2018. The restoring influence of priming treatments on germination of Smooth vetch (Vicia dasycarpa) under drought stress and maintaining this advantage following aging by using post priming heat shock treatment. Iranian Journal of Plant Biology, 10(3): 41-54. (In Persian)(Journal)
Omidi, H., Naghdi Badi, H. A. and Jafarzadeh. L. 2015. Seeds of medicinal plants and crops. Shahed University Press. (Book)
Parera, C. and Cantliffe, D. 1994. Dehydration rate after solid matrix alters seed performance of shrunken-2 corn. Journal of the American Society for Horticultural Science, 119: 629-35. (Journal)
Rajjou, L. and Debeaujon, I. 2008. Seed longevity: survival and maintenance of high germination ability of dry seeds. Comptes Rendus Biologies, 331: 796-805. (Journal)
Roberts, E. H. and Osei-Bonsu, K. 1988. Seed and seedling vigour. In: Summerfield, R. J. (Eds.) World Crops: Cool Season Food Legumes, Kluwer. pp: 897–910
Saadat, H. and Sedghi, M. 2021. Effect of priming and aging on Physiological, biochemical traits seed common bean (Phaseolus vulgaris L.). Journal of Seed Research, 11(3): 75-87. (In Persian)(Journal)
Saadat, T., Alidost, H. and Sedghi, M. 2019. The effect of priming and exhaustion on the germination of rice seed masses with different strength. Journal of Seed Research, 10(4): 60-67. (In Persian)(Journal)
Saadat, T., Sedghi, M., Gholipouri, A., Seyed Sharifi, R. and Sheykhbaglou, R. 2020a. The effect of priming deterioration on the activity of antioxidant enzymes and the mobility of seed reserves in French bean (Phaseolus vulgaris L.) cv. Sadri. Iranian Journal of Seed Science and Technology, 8(2): 19-32. (In Persian)(Journal)
Saadat, T., Sedghi, M., Gholipouri, A., Seyed Sharifi, R. and Sheykhbaglou, R. 2020b. Effect of seed priming and aging on germination, biochemical traits and antioxidant enzyme gene expression in common bean (Phaseolus vulgaris l.). Iranian Journal of Seed Science and Research, 7(1): 1-13. (In Persian)(Journal)
Santos, R. F., Placido, H. F., Bosche, L. L., Neto, H. Z., Ferando, H. and Alessandro, B. 2021. Accelerated aging methodologies for evaluating physiological potential of treated soybean seeds. Journal of Seed Science, 43 (4): 1- 10. (Journal)
Scot, S. J., James, R. A. and Williams, W. A. 1984. Review of data analysis methods for seed germination. Crop Science, 24: 1192-1199. (Journal)
Sedghi, M., Khomari, S. and Amanpour-Balaneji, B. 2011. Effect of Seed Vigor and Hormone Priming on Glyoxylate Cycle Enzymes Activity in Persian Silk Tree (Albizia julibrissin Durazz.). World Applied Science Journal, 13(3): 541-544. (Journal)
Singh, V., Upadhyay, R. S., Sarma, B. K. and Singh, H. B. 2016. Seed bio-priming with Trichoderma asperellum effectively modulate plant growth promotion in pea. International Journal of Agriculture, Environment and Biotechnology, 9(3): 361-365. (Journal)
Siyadat, S. A., Sharafizadeh, M. and Mousavi, S. A. 2011. The effect of priming hormone on the reduction of corn seed burnout. Quarterly journal of plant physiology, 3(10): 83-67. (In Persian)(Journal)
Stephanie, E. B., Svoboda, V. P., Paul, A.T. and Marc, W. V. I.. 2005. Controlled drought affects morphology and anatomy of Salvia solendens. Horticultural Society, 130(5): 775-781. (Journal)
Subramanyam, K., Laing, G. D. and Van Damme, E. J. M. 2019. Sodium selenate treatment using a combination of seed priming and foliar spray alleviates salinity stress in rice. Frontiers in Plant Science, 10: 1-17. (Journal)
Varner, J. E. 1964. Gibberlic acid controlled synthesisof α-amylase in barley endosperm. Plant Physiology, 39: 413-415. (Journal)
Zhan, J., Li, W., He, H. Y., Li, C. Z. and He, L. F. 2014. Mitochondrial alterations during Alinduced PCD in peanut root tips. Plant Physiology and Biochemistry, 75: 105-113. (Journal)
Zheng, M., Tao, Y., Hussain, S., Jiang, Q., Peng, S., Huang, J. and Nie, L. 2016. Seed priming in dry direct-seeded rice: consequences for emergence, seedling growth and associated metabolic events under drought stress. Plant growth regulation, 78(2): 167-178. (Journal)