اثر پیش‌تیمار سدیم نیتروپروساید بر بهبود جوانه‌زنی و رشد اولیه سیاهدانه (Nigella sativa) تحت تنش شوری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه تولیدات گیاهی دانشکده کشاورزی بردسیر، دانشگاه شهید باهنر کرمان، کرمان، ایران

2 دانش‌آموخته دکتری زراعت- فیزیولوژی گیاهان زراعی دانشگاه ایلام، ایلام، ایران

چکیده

به­منظور بررسی تاثیر سدیم نیتروپروساید بر برخی شاخص‌های جوانه‌زنی و فیزیولوژیک گیاه دارویی سیاهدانه (Nigella sativa) تحت تنش شوری، آزمایشی به­صورت فاکتوریل در قالب طرح پایه کامل تصادفی با سه تکرار در آزمایشگاه تحقیقاتی دانشکده کشاورزی، دانشگاه شهید باهنر کرمان اجرا شد. تیمارها شامل، سدیم نیتروپروساید در سه سطح (0، 50 و 100 میکرو‌مولار) به­عنوان عامل اول و تنش شوری با سه سطح (0، 50 و 100 میلی‌مولار) به­عنوان عامل دوم بودند. نتایج آزمایش نشان داد که افزایش تنش شوری باعث کاهش تمام شاخص­های جوانه‌زنی (درصد و سرعت جوانه‌زنی، شاخص بنیه بذر، طول ریشه‌چه و ساقه‎چه، وزن تر و خشک ریشه‌چه، ساقه‌چه و گیاهچه و محتوای نسبی آب برگ) شد، در حالی­که محتوی پرولین در سطح شوری 100 میکرومولار در مقایسه با شاهد، حدود 2/52 درصد افزایش یافت. کاربرد سدیم نیتروپروساید باعث افزایش تمام صفات (به استثنای پرولین که اثر معنی‌داری بر این صفت نداشت) گردید. در بالاترین سطح تنش شوری، بذرهایی که با غلظت 100 میکرومولار سدیم نیتروپروساید پیش­تیمار شده بودند، به­ترتیب باعث افزایش حدود 2/54، 7/47، 6/63 و 2/20 درصد در سرعت و درصد جوانه­زنی، شاخص بنیه بذر و محتوی نسبی آب برگ در مقایسه با بذرهایی که با آب مقطر پیش­تیمار شده بودند، گردید. در نهایت می‌توان بیان کرد که کاربرد این تنظیم‌کننده رشد گیاهی در این آزمایش موجب بهبود شاخص‌های جوانه‌زنی و محتوی نسبی آب گیاهچه سیاهدانه شد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of sodium nitroprusside pretreatment on germination improvement and early growth of black cumin (Nigella sativa) under salinity stress

نویسندگان [English]

  • Rozita Kabiri 1
  • Mehdi Naghizadeh 1
  • Maryam Delfani 2
1 Department of Plant Productions, Agricultural Faculty of Bardsir, Shahid Bahonar University of Kerman, Iran
2 PhD graduated in Agriculture - Crop Physiology, Ilam University. Ilam. Iran
چکیده [English]

To evaluate the effect of sodium nitroprusside on some physiological and germination indices of black cumin (Nigella sativa) under salinity stress, an experiment was conducted in a factorial arrangement based on completely randomized design with three replications at research laboratory of Agricultural Faculty, Shahid Bahonar University of Kerman. Treatments included sodium nitroprusside at three levels (0, 50 and 100 μmol) as the first factor and three levels of salinity stress (0, 50 and 100 mmol) as the second factor. The results showed that increasing salinity stress caused the reduction of all traits of germination index (germination percentage and rate, seed vigor index, root and shoot length, fresh and dry weight of root, stem and seedling and leaf relative water content), while 100 μmol salinity level caused an increasing of 52.2% in proline content as compared to control. Application of sodium nitroprusside increased all traits (except proline, which had no significant effect on this trait). Seeds which were primed with 100 μmol sodium nitroprusside caused an increment of 54.2, 47.7, 63.6, 20.2 % in germination rate and percentage, seed vigor index and relative water content compared with non-treated seeds at the highest level of salinity stress, respectively. Finally, the application of this plant growth regulator in this experiment enhanced germination indices and relative water content of black cumin seedling.

کلیدواژه‌ها [English]

  • Nitric oxide
  • Oxidative damage
  • Proline
  • Seedling growth
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