اثر برازینواستروئید بر صفات مرفولوژیک و فیزیولوژیک آویشن باغی (Thymus vulgaris) تحت تنش شوری

نوع مقاله : مقاله پژوهشی

نویسندگان

استادیار گروه علوم و مهندسی باغبانی و فضای سبز دانشگاه سیدجمال‌الدین اسدآبادی، اسدآباد، ایران

چکیده

پژوهش حاضر با هدف کاهش اثرات تنش شوری بر جوانه­زنی و مراحل اولیه رشد گیاه دارویی آویشن باغی با استفاده از کاربرد برازینواستروئید در شرایط کشت درون­شیشه­ای انجام شد. بذرهای آویشن باغی در معرض تیمار شوری (صفر، 50، 100 و 200 میلی­مولار) و برازینواستروئید (5/0، 1 و 3 میکرومولار)  قرار گرفتند، و سپس شاخص­های گوناگون جوانه­زنی و رشد و نیز برخی ویژگی­های فیتوشیمیایی در گیاهچه­ها اندازه­گیری شدند. نتایج نشان داد که در شرایط شوری، بیش­ترین میزان درصد جوانه­زنی (95 درصد) در پایین­ترین غلظت نمک (50 میلی­مولار) و بالاترین غلظت برازینواستروئید (3 میکرومولار) مشاهده شد. کاربرد غلظت­های مختلف برازینواستروئید باعث بهبود ارتفاع ریشه و ساقه و نیز افزایش میزان وزن خشک و تر گیاهچه­های آویشن باغی شد، به­طوری­که در شوری 200 میلی­مولار، میزان طول ریشه و ساقه در نمونه­های تیمارشده با 3 میکرومولار برازینواستروئید نسبت به نمونه­های تیمارشده با 5/0 میکرومولار برازینواستروئید به­ترتیب حدود 5/30 و 9/42 درصد افزایش یافتند. میزان پرولین و آنزیم­های آنتی­اکسیدانت پراکسیداز (POD) و سوپراکسیددیسموتاز (SOD) در گیاهچه­های تیمارشده با غلظت بالاتر برازینواستروئید نسبت به غلظت­های پایین­تر به­طور معنی­داری افزایش یافتند و این گیاهچه­ها نفوذپذیری غشای (پراکسیده­شدن لیپید) کم­تری داشتند. نتایج ضریب همبستگی نشان داد که بین نفوذپذیری غشای یاخته (پراکسیده­شدن لیپید) و همه صفات بیوشیمیایی و مرفولوژیک اندازه­گیری­شده مربوط به رشد گیاهچه همبستگی منفی وجود دارد. بر اساس نتایج این پژوهش، کاربرد برازینواستروئید با غلظت 3 میکرومولار در مرحله حساس جوانه­زنی بذر و رشد اولیه گیاهچه­های آویشن باغی تحت تنش شوری پیشنهاد می­شود.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of brasinosteroid on morphological and physiological traits of garden thyme (Thymusvulgaris) in salinity stress

نویسندگان [English]

  • Alireza Noroozisharaf
  • Alireza Noroozisharaf
  • Mehrdad Rasouli
Assistant professor of Sayyed Jamaleddin Asadabadi University, Asadabad, Iran
چکیده [English]

The present study was conducted to reduce the effects of salinity stress on germination and initial growth stages of Thymus vulgaris as a medicinal plant using brassinosteroid (BR) under in vitro culture. Thyme seeds were exposed to different concentrations of salinity (0, 50, 100 and 200 mM) and BR (0.5, 1 and 3 µM), and then different germination and growth indices as well as some phytochemical properties of seedlings were measured. Results indicated that under salinity conditions, the highest germination percentage (95%) was observed at the lowest salt concentration (50 mM) and the highest BR concentration (3 µM). Applying differentBR concentrations improved root and shoot height.It also increased dry and fresh biomass of thyme seedlings in a way that at the same salinity conditions of 200 mM, root and stem length increased about 30.5% and 42.9%, respectively, in samples treated with 3 µM BR in coparison with the ones treated with 0.5 µM BR. The amount of proline and antioxidant enzymes, peroxidase (POD) and superoxide dismutase (SOD) significantly increased in the seedlings treated with the highest BR concentration in comparison with those treated with the lower concentrations, and the seedlings had lower membrane permeability (lipid peroxidation). The results of correlation coefficient showed that there was a negative correlation between cell membrane permeability (amount of malondialdehyde) and all measured biochemical and morphological traits related to seedling growth. According to the results of this study, it is suggested to apply BR during the sensitive stage of seed germination and early growth of garden thyme seedlings under water or soil salinity conditions.

کلیدواژه‌ها [English]

  • Peroxidase
  • Saltstress
  • Membrane permeability
  • In vitro culture
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