ارزیابی قابلیت پرایمینگ بذر با سلنیوم برای بهبود بذرهای فرسوده عدس (Lens culinaris Medic)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، پردیس کشاورزی و منابع طبیعی، دانشگاه رازی، کرمانشاه، ایران

2 دانشیار، گروه مهندسی تولید و ژنتیک گیاهی، پردیس کشاورزی و منابع طبیعی، دانشگاه رازی، کرمانشاه، ایران

چکیده

بذر گیاهان طی انبارداری، زوال می‏یابد. هدف اصلی این پژوهش، بررسی اثر سلنیوم در بهبود بذرهای زوال­یافته دو رقم عدس بود. تحقیق به­صورت آزمایش فاکتوریل بر پایه طرح کاملاً تصادفی اجرا شد. بذر دو رقم عدس (کیمیا و بیله­سوار) در شرایط مختلف فرسودگی (بدون فرسودگی، فرسودگی ملایم و فرسودگی شدید) قرار گرفتند. سپس این بذرهای فرسوده، با غلظت­های سلنیوم (صفر، 250، 500 و 750 میکرومولار) پرایمینگ شدند. پس از پرایمینگ، بذرها در آزمون جوانه­زنی استاندارد قرار گرفته و صفات مرتبط اندازه­گیری شدند. نتایج نشان داد که در شرایط بدون فرسودگی، پرایمینگ بذر با سلنیوم، تأثیر معنی­داری بر خصوصیات جوانه‏زنی و بنیه بذر و گیاهچه نداشت. در شرایط فرسودگی ملایم و شدید، با افزایش غلظت سلنیوم از صفر تا 500 میکرومولار، تغییر معنی­داری در اکثر خصوصیات جوانه­زنی مشاهده نگردید، اما غلظت 750 میکرومولار سبب افزایش این ویژگی‏ها شد. مثلاً، در شرایط فرسودگی ملایم و شدید پرایمینگ بذر در غلظت 750 میکرومولار سبب افزایش سرعت جوانه‏زنی به­ترتیب 30 و 62 درصد و کاهش متوسط زمان جوانه‏زنی به­ترتیب 13 و 17 درصد گردید. از نظر فعالیت آنزیم‏های کاتالاز، پراکسیداز و سوپراکسید دیسمیوتاز مشخص شد که در بذرهای غیرفرسوده، پرایمینگ بذر با سلنیوم در غلظت 750 میکرومولار باعث افزایش فعالیت این سه آنزیم به­ترتیب 81، 19 و 43 درصد شد. تأثیر پرایمینگ بذر در غلظت 750 میکرومولار بر فعالیت این سه آنزیم برای شرایط فرسودگی ملایم به­ترتیب 221، 92 و 86 درصد و برای شرایط فرسودگی شدید به­ترتیب 376، 143 و 141 درصد نسبت به شاهد مربوطه بود. همچنین، روند تأثیر پرایمینگ بذر با سلنیوم بر صفات مطالعه­شده، برای دو رقم عدس کیمیا و بیله‏سوار مشابه بود. بنابراین، به نظر می­رسد که پرایمینگ بذر با سلنیوم در غلظت 750 میکرومولار، قابلیت بهبود بذرهای زوال­یافته عدس رقم‏های کیمیا و بیله سوار را داشته باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation the ability of seed priming with selenium to improving deteriorated seeds in lentil (Lens culinaris Medic)

نویسندگان [English]

  • Mahtab Mehrkish 1
  • Mokhtar Ghobadi 2
  • Saeed Jalali Honarmand 2
1 MSc student
2 Associate Professor
چکیده [English]

Seeds of crops deteriorate during storage. The main aim of this study was to investigate the effect of selenium on the improvement of deteriorated seeds in two lentil cultivars. The research was conducted as a factorial experiment based on a completely randomized design. The seeds of two lentil cultivars (Kimia and Bilehsavar) were exposed to different deterioration conditions (no deterioration, mild and severe deterioration). Then, these deteriorated seeds were primed by different concentrations of selenium (0, 250, 500 and 750 µM). After the priming, the seeds were evaluated in a standard germination test and related traits were measured. In non-deteriorated seeds, priming with selenium did not have significant effects on the seed germination and seedling vigor characteristics. Under mild and severe deterioration conditions, seed priming with selenium at 0, 250, and 500 μM had not significant differences in most seed and seedling vigor characteristics, but priming at 750 μM improved this traits. For example, under mild and severe seed deterioration conditions, seed priming at 750 μM selenium increased the germination rate by 30 and 62 %, respectively, and decreased the mean germination time by 13 and 17 %, respectively. In terms of the activity of catalase, peroxidase and superoxide dismutase enzymes, it was found that in non-deteriorated seeds, the activity of these enzymes were increased 81, 19 and 43% respectively; by seed priming with selenium 750 μM. The effect of seed priming at 750 μM selenium on activity of these three enzymes was 221, 92 and 86% for mild seed deterioration and 376, 143 and 141% for severe seed deterioration conditions, respectively. Also, the effect of seed priming with selenium on the studied traits was similar for the two lentil cultivars Kimia and Bilehsavar. Therefore, it seems that seed priming with selenium 750 μM had the ability of improvement the deteriorated lentil seeds Kimia and Bilehsavar.

کلیدواژه‌ها [English]

  • Enzymatic activity
  • Germination
  • Seed deterioration
  • Seed pre-treatment
  • Seedling vigor
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