بررسی تاثیر اسید سالسیلیک بر جوانه‌زنی و فعالیت برخی آنزیم‌های آنتی‌اکسیدانی سیاه‌دانه (Nigella sativa L.) در شرایط تنش شوری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دکتری مهندسی ژنتیک، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه ایلام، ایلام، ایران

2 استادیار، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه ایلام، ایلام، ایران

3 دانشجوی دکتری مهندسی ژنتیک، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه ایلام، ایلام، ایران

چکیده

به­منظور بررسی تاثیر پیش‌تیمار اسید سالسیلیک بر ویژگی‌های جوانه‌زنی در شرایط تنش شوری، آزمایشی به­صورت فاکتوریل در قالب طرح پایه کاملاً تصادفی با سه تکرار انجام گرفت. فاکتورهای آزمایش شامل اسید سالسیلیک در سه سطح (صفر، 75/0و 5/1 میلی‌مولار) و تنش شوری در پنج سطح (صفر، 100، 200، 300 و 400 میلی‌مولار) بودند. اثرات ساده اسید سالسیلیک و تنش شوری بر صفات شاخص بنیه بذر، وزن تر و وزن خشک گیاهچه معنی‌دار بودند. اثرات متقابل اسید سالسیلیک و شوری بر سایر صفات در سطح احتمال یک درصد معنی‌دار بود. نتایج نشان داد که شوری به­طور معنی‌داری جوانه‌زنی و رشد گیاهچه را کاهش، اما اسید سالسیلیک شاخص‌های جوانه‌زنی را بهبود بخشید و در تیمارهای تنش­دیده سبب افزایش جوانه‌زنی شد. با افزایش سطح تنش شوری سرعت و درصد جوانه‌زنی کاهش یافت. بیش­ترین سرعت جوانه‌زنی در تیمار 5/1 میلی‌مولار اسید سالسیلیک در شرایط بدون شوری حاصل شد. پیش­تیمار موجب افزایش معنی‌دار میزان فعالیت آنزیم کاتالاز نسبت به بدون پیش­تیمار و کاهش میزان مالون­دی‌آلدئید در تیمارهای دارای اسید سالسیلیک گردید. پیش­تیمار با غلظت 5/1 میلی‌مولار ضمن این­که تاثیر بیش­تری بر جوانه‌زنی داشت، سبب افزایش مقاومت گیاهچه‌های سیاه‌دانه تحت تنش شوری نیز شد.

کلیدواژه‌ها


عنوان مقاله [English]

Investigating effect of salicylic acid on germination and activity of some enzymes antioxidant of Nigella sativa L. under salt stress conditions

نویسندگان [English]

  • Batool Zarei 1
  • Arash Fazeli 2
  • Zahra Taghipour 3
1 Ph.D of Genetic Engineering, Department of Agronomy and Plant Breeding, College of Agriculture, Ilam University, Ilam, Iran
2 Assistant Professor, Department of Agronomy and Plant Breeding, College of Agriculture, Ilam University, Ilam, Iran
3 Ph.D Candidate of Genetic Engineering, Department of Agronomy and Plant Breeding, College of Agriculture, Ilam University, Ilam, Iran
چکیده [English]

 In order to investigate the effect of salicylic acid on germination properties under salinity conditions, a factorial design based on complementary randomized design with three replications. The test factors consisted of salicylic acid at three levels (zero, 0.75 and 1.5 millimolar) and salinity stress at five levels (0, 100, 200, 300 and 400 millimolar). The effects of salicylic acid and salinity stress on seed vigor index, fresh weight and seedling dry weight were significant. The effects of salicylic acid and salinity on other traits were significant at 1% probability level. The results showed that salinity significantly reduced germination and seedling growth, but salicylic acid improved germination indices and increased germination in stressed treatments. With increasing salinity stress, the rate and germination percentage decreased. The maximum rate of germination was obtained in saline silicic acid (1.5mM) treatment under non salinity conditions. Pre-treatment significantly increased the activity of catalase enzyme compared to pretreatment and decreased malondialdehyde in salicylic acid treatments. Pre-treatment at a concentration of 1.5 (mM), while also having more effect on germination, increased the resistance of seedlings of Nigella sativa under salinity stress.

کلیدواژه‌ها [English]

  • Catalase
  • Germination
  • Malondialdehyde
  • Salicylic acid
  • Salinity stress
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