بررسی برخی خصوصیات مرتبط با قابلیت جوانه‌زنی و بنیه گیاهچه بادام‌زمینی رقم NC2 در بذرهای حاصل از بوته‌های محلول‌پاشی‌شده با متانول و متیلوباکتریوم

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموخته دکترای زراعت، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران

2 دانشیار گروه زراعت و اصلاح نباتات، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران

3 دانشیار گروه زراعت و اصلاح نباتات، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران

4 استاد گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران

چکیده

به­منظور بررسی خصوصیات مرتبط با قابلیت جوانه­زنی و بنیه گیاهچه بادام­زمینی در بذرهای حاصل از بوته­های محلول­پاشی­شده با متانول و متیلوباکتریوم تحقیقی آزمایشگاهی در 2 سال در دانشگاه آزاد اسلامی واحد رشت به اجرا درآمد. این تحقیق با استفاده از آزمون­های جوانه­زنی استاندارد، سرما و پیری تسریع­شده اجرا شد. هر یک از آزمون­ها با استفاده از آزمایش فاکتوریل بر پایه طرح بلوک­های کامل تصادفی با 16 تیمار (جمعیت باکتری متیلوباکتریوم بر حسب CFU در 4 سطح صفر، 106، 108 و 1012 و 4 سطح مقدار متانول صفر، 10، 20 و 30 درصد حجمی) در 3 تکرار اجرا شد. خصوصیات مورد بررسی عبارت بودند از درصد جوانه­زنی نهایی، بنیه گیاهچه، وزن خشک ریشه­چه، وزن خشک هیپوکوتیل، وزن خشک ساقه­چه و وزن خشک گیاهچه. نتایج نشان داد که اثر سال بر تمام خصوصیات مورد بررسی در کلیه آزمون­ها معنی­دار بود. جمعیت باکتری فقط اثر معنی­داری بر درصد جوانه­زنی نهایی در کلیه آزمون­ها داشت. اما مقدار متانول اثر معنی­داری بر تمام خصوصیات مورد بررسی در هر سه آزمون داشت. اثر متقابل جمعیت باکتری در مقدار متانول نیز فقط بر درصد جوانه­زنی نهایی در آزمون­های جوانه­زنی استاندارد و سرما اثر معنی­داری داشت. مقایسه میانگین صفات مورد بررسی نیز نشان داد که بیش­ترین مقدار درصد جوانه­زنی نهایی در کلیه آزمون­ها در جمعیت باکتری CFU 1012 مشاهده شد. بیش­ترین مقدار تمام خصوصیات بررسی­شده در کلیه آزمون­ها از مقدار 20 درصد حجمی متانول به­دست آمد.

کلیدواژه‌ها


عنوان مقاله [English]

Study of some characteristics related to germinability and seedling vigour of peanut (Arachis hypogaea L.) seeds cultivar NC2 from plants had been sprayed with methanol and mthylobacterium

نویسندگان [English]

  • AliReza Hossinzadeh Gashti 1
  • Vorham Rashidi 2
  • Mohammad Naghi Safarzad Vishgahi 3
  • Masoud Esfehani 4
  • Farhad Farahvash 2
1 Ph.D Candidate of Agronomy, Tabriz Branch, Islamic Azad University, Tabriz, Iran
2 Associate Professor, Department of Agronomy and Plant Breeding, Tabriz Branch, Islamic Azad University, Tabriz, Iran
3 Associate Professor, Department of Agronomy and Plant Breeding, Rasht Branch, Islamic Azad University, Rasht, Iran
4 Professor, Department of Agronomy and Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
چکیده [English]

In order to evaluate Germinability and seedling vigour associated with peanut seeds of the plants had been sprayed with methanol and mthylobacterium a laboratory research at 2 years was conducted in Islamic Azad University of Rasht. This study was conducted using standard germination test, cold and accelerated aging test. Each test using factorial experiment based on randomized complete block design with 16 treatments (bacteria populations mthylobacterium in unit of CFU in 4 levels, 106, 108 and 1012 and 4 levels of methanol 0, 10, 20 and 30 % v/v) in three replications. characteristics studied were: final germination percentage, seedling  vigour, root dry weight, hypocotyl dry weight , shoot dry weight and seedling dry weight. The results showed that the effect of year was significant on all studied characteristics in all the tests. Bacteria population only significant effect on the final germination percentage in all the tests. The amount of methanol had significant effect on all studied characters in the three tests. Interaction between the bacterial population with amount of methanol only the final germination percentage in standard germination and cold tests had a significant effect. Comparison of means showed the highest final germination percentage in the population of bacteria 1012 CFU was observed in all the tests. Most of all investigated characteristics in all tests was 20 percent methanol.

کلیدواژه‌ها [English]

  • Methanol rate
  • Methylobacterium concentration
  • Peanut
  • Seed tests
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