اثر پرایمینگ با اسیدسالیسیلیک و اسیدآسکوربیک بر شاخص‌های جوانه‌زنی و صفات بیوشیمیایی گندم (Triticum aestivum L) رقم پیشتاز در شرایط زوال بذر

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکترای زراعت، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، ایران

2 دانشیار، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، ایران

چکیده

هدف از انجام این آزمایش بررسی اثر اسیدسالیسیلیک و اسیدآسکوربیک بر بهبود خصوصیات جوانه­زنی و کیفیت فیزیولوژیک بذرهای زوال­یافته گندم (Triticum aestivum L.) بود.  تیمارهای آزمایش شامل دو سطح پیری تسریع­شده و شرایط نرمال و تنظیم­کننده رشد گیاهی اسیدسالیسیلیک و اسیدآسکوربیک در سه سطح 50، 100 و 150 قسمت در میلیون و هیدروپرایمینگ بود.  تیمار بدون پرایمینگ به‌عنوان شاهد استفاده شد. آزمایش به­صورت فاکتوریل در قالب طرح کاملا تصادفی در سه تکرار انجام شد. صفات مورد بررسی در این پژوهش شامل درصد جوانه­زنی، سرعت جوانه­زنی، میانگین زمان جوانه­زنی، شاخص طولی بنیه گیاهچه، شاخص جوانه­زنی، ضریب سرعت جوانه­زنی، هدایت الکتریکی بذرها، فعالیت آنزیم­های آنتی‌اکسیدانت کاتالاز، پراکسیداز و سوپراکسید دیسموتاز و محتوای مالون­دی­آلدهید، پروتئین محلول، قند محلول و پرولین بودند. نتایج نشان داد که اثر متقابل زوال در پرایمینگ برای درصد جوانه­زنی معنی­دار نبود درحالی­که برای صفات میانگین زمان جوانه­زنی، سرعت جوانه­زنی، شاخص جوانه­زنی و ضریب سرعت جوانه­زنی در سطح احتمال یک درصد و برای شاخص طولی بنیه گیاهچه در سطح احتمال پنج درصد معنی­دار بود. در این پژوهش بهترین تیمار برای بهبود خصوصیات جوانه­زنی بذرهای زوال­یافته استفاده از اسیدآسکوربیک 100 بود که 23 درصد توانست جوانه­زنی را بهبود بخشد. در شرایط نرمال نیز بیش­ترین درصد جوانه­زنی اسیدسالیسیلیک 100 به­دست آمد که سبب افزایش 31/22 درصدی در جوانه­زنی نهایی گردید. همچنین نتایج نشان داد که پیری تسریع­شده به­طور معنی­داری فعالیت آنزیم­های آنتی‌اکسیدانت و پروتئین محلول را کاهش داده و در مقابل سبب افزایش معنی­داری در هدایت الکتریکی و محتوای مالون­دی­آلدهید، قند محلول و پرولین می­شود.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of priming with salicylic acid and ascorbic acid on germination indices and biochemical traits in wheat seed deterioration

نویسندگان [English]

  • Saeed Moori 1
  • Hamid Reza Eisvand 2
1 PhD Student of Agronomy, College of Agriculture, Lorestan University, Khorramabad, Iran
2 Associate Professor, College of Agriculture, Lorestan University, Khorramabad, Iran
چکیده [English]

Wheat as the most important plants on earth and is the main source of feeding people in Iran. Damage of seed deterioration as the most important factor has been proposed by researchers. The purpose of this research is to evaluate the effects of plant growth regulators (salicylic acid and vitamin C) to improve germination and physiological quality of deteriorated seeds in wheat (Triticum aestivum), respectively. The treatments included two levels of accelerated aging in normal conditions and plant growth regulators included salicylic acid and ascorbic acid at levels of 50, 100 and 150 ppm and hydropriming. No priming treatment was as a control. A factorial experiment in a completely randomized design with three replications was done. In this research, the best treatments to improve seed germination characteristics crumbling use of ascorbic acid 100 ppm that 23% could improve germination. Under normal conditions, the highest percentage of germination was obtained salicylic acid 100 ppm 22.31%  increase in germination was finalized. The results showed that significantly accelerated aging antioxidant enzyme activity and soluble protein reduced and in contrast, a significant increase in electrical conductivity and MDA content, soluble sugar and proline to be.

کلیدواژه‌ها [English]

  • Accelerated Aging
  • Antioxidant Enzyme
  • Soluble Organic Solute
  • Wheat Seedling
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