اثر متیل جاسمونات بر شاخص‌های جوانه‌زنی، پرولین و آنزیم پراکسیداز در رازیانه ((Foeniculum vulgare Mill تحت تنش کلرید سدیم

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد زراعت، دانشگاه آزاد اسلامی واحد شیراز، شیراز، ایران

2 استادیار، گروه علوم باغبانی، دانشگاه آزاد اسلامی واحد شیراز، شیراز، ایران

3 کارشناس سازمان جهاد کشاورزی استان فارس، شیراز، ایران

چکیده

این پژوهش به­منظور بررسی تاثیر پرایمینگ بذر بر برخی خصوصیات رازیانه در مرحله جوانه­زنی، به­صورت فاکتوریل در قالب طرح کاملاً تصادفی با 3 تکرار در سال 94-1393 در دانشکده کشاورزی دانشگاه آزاد اسلامی شیراز انجام شد. فاکتورهای آزمایش شامل فاکتور اول (A): متیل جاسمونات در سه سطح (صفر، 500 و 1000 میلی­گرم در لیتر) و فاکتور دوم (B): کلرید سدیم در 4 سطح (صفر، 5/2، 5 و 5/7 دسی­زیمنس بر متر) بود. صفات مورد بررسی شامل درصد و سرعت جوانه­زنی، محتوای پرولین و میزان آنزیم پراکسیداز بود. نتایج این پژوهش نشان داد کاربرد غلظت 1000 میلی­گرم در لیتر متیل جاسمونات باعث شد درصد جوانه­زنی در شرایط بدون تنش (غلظت صفر کلرید سدیم) بیش-ترین مقدار (100 درصد) را داشته باشد. همچنین پیش­تیمار بذرهای رازیانه توسط غلظت 500 و 1000 میلی­گرم در لیتر متیل جاسمونات باعث افزایش سرعت جوانه­زنی در شرایط نرمال شد. کاربرد غلظت 1000 میلی­گرم در لیتر متیل جاسمونات باعث شد بیش­ترین محتوای پرولین در شرایط تنش شوری متوسط (5 دسی­زیمنس بر متر کلرید سدیم) و شدید (غلظت 5/7 دسی­زیمنس بر متر کلرید سدیم) مشاهده گردید. بر اساس نتایج حاصل از اثر متقابل پرایمینگ و غلظت­های مختلف کلرید سدیم مشخص شد پیش­تیمار بذرهای رازیانه توسط متیل جاسمونات با غلظت 500 میلی­گرم در لیتر باعث افزایش فعالیت آنزیم پراکسیداز در شرایط تنش شوری شدید (غلظت 5/7 دسی­زیمنس بر متر) شد. در مجموع، نتایج حاصل نشان داد که پیش­تیمار بذر توسط متیل جاسمونات در مناطق مستعد تنش شوری می­تواند باعث مقاومت بذر گیاه رازیانه در مرحله جوانه­زنی شود.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of methyl jasmonate on Germination index, proline and peroxidaze enzyme activity at fennel (Foeniculum vulgare Mill) under NaCl stress

نویسندگان [English]

  • Reza Zeinali 1
  • Farshad Sadeghi 2
  • Marziyeh Dehghan 3
1 MSc student, Department of Horticultural Science, Shiraz Branch, Islamic Azad University, Shiraz, Iran
2 Assistant Professor, Department of Horticultural Science, Shiraz Branch, Islamic Azad University, Shiraz, Iran
3 Expert, Agricultural Organization of Fars Province, Shiraz, Iran
چکیده [English]

This study was carried outfennel (Foeniculum vulgare Mill) under NaCl stress, seed germination with Methyl jasmonate priming. a factorial experiment  at CRD design with three replications in 2014-2015 at the Faculty of Agriculture, Islamic Azad University of Shiraz. the factor A:includes Methyl jasmonate(MeJa) at three levels (0, 500 and 1000 mg/l) and factor (B): sodium chloride(NaCl) at 4 levels (0, 2.5, 5 and 7.5 dS/m). The characteristics such as Rate and percentage of germination, proline content and peroxidase. The results indicated that concentration of 1000 mg/l of MeJa led to increased germination under Control (100 %). Priming of Fennel seeds at 500 and 1000 mg/l of MeJa increase the germination rate were in Control. 1000 mg/l of MeJa led to the largest concentration of proline content in moderate NaCl stress conditions (5 dS/m) and severe (7.5 dS/m) is observed. Based on the results obtained from the interaction between priming and different concentrations of NaCl was found that priming of fennel seeds by 500 mg/l of MeJa increase peroxidase activity at 7.5 dS/m of NaCl. Finally, the results showed that Priming of fennel seeds by MeJa improve seed index, proline and peroxidaze enzyme activity at NaCl stress.

کلیدواژه‌ها [English]

  • Fennel
  • Germination index
  • NaCl
  • Peroxidase
  • Proline
Akhkha, A., Boutra, T. and Alhejely, A. 2011. The rates of photosynthesis, chlorophyll content, dark respiration, proline and abscicic acid (ABA) in wheat (Triticum durum) under water deficit conditions. International Journal of Agricultural and Biological Engineering, 13: 215- 221. (Journal)
Ansari, O., Choghazardi, H.R., Sharif Zadeh, F. and Nazarli, H. 2012. Seed reserve utilization and seedling growth of treated seeds of mountain rye (Secalemontanum) as affected by drought stress. Cercetări Agronomiceîn Moldova, 2(150): 43-48. (In Persian)(Journal)
Asada, K. 1999. The water-water cycle in chloroplasts: scavenging of active oxygen’s and dissipation of excess photons. Annual Review of Plant Biology, 50: 601-639. (Journal)
Ashraf, M. and Foolad, M.R. 2005. Pre-sowing seed treatment shotgun approach to improve germination, plant growth, and crop yield under saline and non-saline conditions. Advances in Agronomy, 88: 223–271. (Journal)
Bates, I.S., Waldern, R.P. and Teare, I.D. 1973. Rapid determination of free proline for water stress studies. Plant Soil, 39: 205-207. (Journal)
Burnett, S., Thomas, P. and Van Iersel, M. 2005. Postgermination drenches with PEG-8000. reduce
growth of salvia and marigolds. Horticultural Science, 40: 675-679. (Journal)
Darzi, M.T. and Haj Sayed Hadi, M.R. 2002. Agronomic and ecological issues in chamomile and fennel. Olive Magazine, 152: 43-49. (In Persian)(Journal)
Dolatabadian, A., Modares sanavi, M.A. and Etemadi, F. 2008. Effects of salicylic acid pretreatment on wheat seed germination (Triticum aestivum L.) under salt stress. Iranian Journal of Biology, 21(4): 692-702. (In Persian)(Journal)
El-Keblawy, A. and Al-Rawai, A. 2005. Effects of salinity, temperature and light on germination of invasive Prosopisjuliflora. Journal of Arid Environments, 61: 555-565. (Journal)
Fallahi, J., Ebadi, M.T. and Ghorbani, R. 2009. The effects of salinity and drought stresses on germination and seedling growth of clary. Environmental Stresses in Agricultural Sciences, 1(1), 57-67. (In Persian)(Journal)
Ghanati, F., Morita, A. and Yokota, H. 2002. Induction of suberin and increase of liginin content by excess Boron in Tabacco cell. Soil Science and Plant Nutrition, 48(3): 357-364. (Journal)
Hayouni, E.A., Chraief, I., Abedrabba, M., Bouix, M., Leveau, J., Mohammed, H. and Hamdi, M. 2008. Tunisian Salvia officinalis L. and Schinus molle L. essential oils: Their chemical compositions and their preservative effects against Salmonella inoculated in minced beef meat. International Journal of Food Microbiology, 125: 242–251. (Journal)
Kaur, S., Gupta, K.A. and Kaur, N. 1998. Gibberellic acid and kinetin partially reverse the effect of water stress on germination and seedling growth in chickpea. Plant Growth Regulation, 25: 29-33. (Journal)
Miri, H.R. 2009. Plant stress physiology. Kermanshah Branch, Islamic Azad University Press. 472p. (In Persian)(Book)
Mohammadi, H. 2013. The Role of priming on seed reserve utilization and germination of barley (Hordeum vulgare L.) seeds under drought stress. International Journal of Agronomy and Plant Production, 4(10): 2543-2547. (Journal)
Patade, V.Y., Maya, K. and Zakwan, A. 2011. Seed priming mediated germination improvement and tolerance to subsequent exposure to cold and salt stress in capsicum. Journal of Seed Science, 4(3): 125 -136. (Journal)
Poljakoff-Maybo, A., Somers, G.F., Werker, E. and Galagher, M. 1994. Seeds of Kosteletzkyavirginica (Malvaceae): Their structure, germination and salt tolerance. American Journal of Botany, 81: 54- 59. (Journal)
Radosevich, S., Holt, J. and Ghersa, C. 1997. Weed Ecology Implications for Management. New York: Wiley. (Book)
Rajabi, R. and Postini, K. 2005. Effect of NaCl on thirty cultivars of bread wheat seed germination. Agricultural Science, 27: 1. 29-45. (In Persian)(Journal)
Salimi, F. and Shekari, F. 2012. The effect of methyl jasmonate and salinity on some morphological characteristics and flower yield of German chamomile (Matricaria chamomilia L.). Plant Biology, 4(11): 27-38. (In Persian)(Journal)
Shariatmadary, M.H., Golbashy, M., ShoaHosiny, M., JamalyRafsanjan, M. and Zamni-Than, R.K. 2009. Study the effect level different of salt stress (NaCl) on germination and growth of Fleawort. The Conference of Water, Soil, Plant and Agriculture, Dezful, Iran. (In Persian))Conference)
Sheykhbaglou, R., Rahimzadeh, S., Ansari, O. and Sedghi, M. 2014. The effect of salicylic acid and gibberellin on seed reserve utilization, germination and enzyme activity of Sorghum (Sorghum bicolor L.) seeds under drought stress. Journal of Stress Physiology and Biochemistry, 10(1): 5-13. (Journal)
Singh, B. and Usha, K. 2003. Salicylic acid induced physiological changes in wheat seedlings under water stress. Plant Growth Regulations, 39: 137- 141. (Journal)
Tabatabaee, S.A. 2013. Effects of maize seed treatments on germination indices and trends of drug storage in saline conditions. Science and Technology Seed, 3(4): 44-51. (Journal)
Tipirdamaz, R. and Gomurgen, N. 2000. The effects of temperature and gibberellic acid on germination of Eranthis hyemalis (L.) Salisb. Seeds. Turkish Journal of Botany, 24: 143-145. (Journal)
Wang, L.J. and Li, S.H. 2006. Thermo tolerance and related antioxidant enzyme activities induced by heat accumulation and salicylic acid in grape (Vitis vinifera L.) leaves. Plant Growth Regulations, 48: 137-144. (Journal)