تاثیر نانو دی‌اکسید تیتانیوم و نیترو پروساید سدیم بر شاخص‌‌های جوانه‌زنی بذر، بنیه و آنزیم‌های آنتی‌اکسیدانت گیاهچه‌ی جو افضل تحت تنش شوری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی دانشگاه بوعلی سینا

2 عضو هیات علمی گروه زراعت و اصلاح نباتات دانشکده کشاورزی دانشگاه بوعلی سینا

چکیده

شوری از مهم­ترین عوامل محدود کننده رشد و عملکرد گیاهان زراعی در بسیاری از مناطق دنیا است. به منظور بررسی اثر نانو دی­اکسید تیتانیوم و نیترو پروساید سدیم بر شاخص­های جوانه­زنی و میزان آنزیم­های آنتی­اکسیدانت گیاهچه­های جو رقم افضل تحت تنش شوری آزمایشی به صورت فاکتوریل در قالب طرح کاملاً تصادفی با چهار تکرار انجام شد. فاکتورهای مورد مطالعه شامل 1. نانو دی­اکسید تیتانیوم در چهار سطح  (0، 500، 1000 و 2000 میلی­گرم در لیتر)، 2. نیترو پروساید سدیم در دو سطح (0 و 100 میکرو­مولار) و 3. تنش شوری در سه سطح (0، 50 و 100 میلی­مولار کلرید سدیم) بودند. تنش شوری شاخص­های جوانه­زنی بذر مانند درصد و سرعت جوانه­زنی، شاخص بنیه، طول ریشه­چه، طول ساقه­چه، میانگین زمان جوانه­زنی و محتوی رطوبت نسبی برگ در گیاهچه را کاهش و نشت الکترولیتی غشاء را افزایش داد. در حضور نانو دی­اکسید تیتانیوم اکثر شاخص­های جوانه­زنی و فعالیت­ آنزیم­های آنتی­اکسیدانت کاتالاز (CAT)، آسکوربات پراکسیداز (APX) و به ویژه سوپر اکسید دیسموتاز (SOD) افزایش یافتند. کاربرد نیترو پروساید سدیم موجب تعدیل نشت الکترولیتی غشاء (EL) و اثرات منفی تنش­ شوری شد. برهمکنش نانو دی اکسید تیتانیوم (500 میلی­گرم در لیتر) و نیترو پروساید سدیم (100 میکرومولار)، میزان آنزیم­های سوپر اکسید دیسموتاز، آسکوربات پراکسیداز و کاتالاز را تحت تنش 100 میلی­مولار کلرید سدیم به ترتیب 28، 8/16 و 9/17 درصد افزایش داد. به نظر می­رسد نیترو پروساید سدیم در حضور نانو دی­اکسید تیتانیوم از طریق تعدیل اثرات بازدارنده کلرید سدیم، بر بهبود شاخص­های جوانه­زنی و میزان آنزیم­های آنتی اکسیدانت­ گیاهچه­های جو تاثیر مناسب داشته باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Nano Titanium Dioxide and Sodium nitroprusside on seed germination, vigor index and antioxidant enzymes of Afzal barley seedling under salinity stress

نویسندگان [English]

  • Afshin Karami 1
  • Ali Sepehri 2
چکیده [English]

Salinity is the most important factor limiting growth and yield of crops in many area of the world. In order to study the effects of nano-TiO2 (0, 500, 1000, 2000 mg/L) and sodium nitroprusside (0 and 100 μM) on seed germination and antioxidant content of barley (var. Afzal) under salinity stress (0, 50 and 100 mM Sodium chloride) a factorial experiment based on a completely randomized design with four replications was conducted. Salinity stress declined the germination indices such as, germination percentage, germination rate, radicle length, plumule length, vigor index, mean germination time, relative water content and increased the membrane electrolyte leakage. In the presence of nano-TiO2, most of germination indices improved and the content of CAT, APX and especially SOD enzymes increased. Use of Sodium nitroprusside diminished the adverse effects of salinity stress and decreased the membrane electrolyte leakage. Also the interaction effect of sodium nitroprusside and nano-TiO2 was significant on the most studied traits. Nano-TiO2 (500 mg/L) with SNP improved SOD, APX and CAT enzyme contents by about 28, 16.8 and 17.9 % under 100 Mm salinity stress. According to the results, it seems that sodium nitroprusside diminished the adverse effects of salinity stress and impressed the nano-TiO2 performance on barley germination indices and antioxidant enzymes content.

کلیدواژه‌ها [English]

  • Nano particle
  • Sodium chloride
  • Stress ameliorator
Abdul-Baki, A. A. and Anderson, J. D. 1973. Vigour determination in soybean by multiple criteria. Crop Science. 10: 4-31. (Journal)
Baiazidi Aghdam,M. T., Mohammadi,H. and Ghorbanpour, M. 2016. Effects of nanoparticulate anatase titanium dioxide on physiological and biochemical performance of Linum usitatissimum (Linaceae) under well-watered and drought stress conditions. Brazilian Journal of Botany. 39: 139-146. (Journal)
Bajji, M., Kinet, J. M. and Lutts, S. 2002. Osmotic and ionic effects of NaCl on germination, early seedling growth, and ion content of Atriplex halimus (Chenopodiaceae). Canadian Journal of Botany. 80: 297-304. (Journal)
Besson-Bard, A., Courtois, C., Gauthier, A., Dahan, J., Dobrowolska, G., Jeandroz, S. and Wendehenne, D. 2008. Nitric oxide in plants: production and cross-talk with Ca2+ signaling. Molecular Plant. 1: 218-228. (Journal)
 
Bowler, C., Slooten, L., Vandenbranden, S., De Rycke, R., Botterman, J., Sybesma, C., Van Montagu, M. and Inzé, D. 1991. Manganese superoxide dismutase can reduce cellular damage mediated by oxygen radicals in transgenic plants. The EMBO Journal. 10: 1723-1732. (Journal)
Britton, C. and Mehley, A. 1955. Assay of catalase and peroxidase. Methods in Enzymology. 2: 764-775. (Journal)
Chutipaijit, S. 2015. Establishment of condition and nano particle factors influencing plant regeneration from aromatic rice (Oryza sativa). International Journal of Agriculture & Biology. 17: 1049-1054. (Journal)
Djanaguiraman, M. and Prasad, P. V. 2013. Effects of salinity on ion transport, water relations and oxidative damage. In Ecophysiology and Responses of Plants under Salt Stress. Springer New York. pp: 89-114.
Ellis, R. H. and Roberts, E. H. 1981. The quantification of ageing and survival in orthodox seeds. Seed Science and Technology. 9: 377-409. (Journal)
Farahani, H. A., Sani, B. and Maroufi, K. 2012. The germination variations in fleawort (Plantago psyllium L.) by nano-particle. International conference on biotechnology, nanotechnology and its application. 17-18 March, Bangkok, Thailand. pp: 43-46. (Journal)
Fathi Amirkhiz, K., Omidi, H., Heshmati, S. and jafarzadeh, L. 2012. Expedited review on the vigor of the herb black seed germination characteristics (Nigella sativa L.) under salt stress. Iranian Journal of Field Crops Research. 10: 299-310. (In Persian) (Journal)
Feizi, H., Kamali, M., Jafari, L. and Rezvani-Moghaddam, P. 2013. Phytotoxicity and stimulatory impacts of nanosized and bulk titanium dioxide on fennel (Foeniculum vulgare). Chemosphere. 91: 506-511.(In Persian) (Journal)
Frazier, T. P., Burklew, C. E. and Zhang, B. 2014. Titanium dioxide nanoparticles affect the growth and microRNA expression of tobacco (Nicotiana tabacum). Funct Integr Genomics, 14: 75-83.
Ghadi, N. A. 2014. Invest age of effects of Sodium Nitroprusside on Plant (Review). International Journal of Agriculture and Crop Sciences. 7: 610-615. (Journal)
Gogos, A., Knauer. K. and Bucheli, T. 2012. Nanomaterials in plant protection and fertilization: current state, foreseen applications, and research priorities. Journal of Agricultural and Food Chemistry. 60: 9781-9792. (Journal)
Guo, Y., Tian, Z., Yan, D., Zhang, J. and Qin, P. 2009. Effects of nitric oxide on salt stress tolerance in Kosteletzkya virginica. Life Sciences Journal. 6: 67-75. (Journal)
Hashemi Dehkourdi, E. and Mosavi, M. 2013. Effect of anatase nanoparticles (TiO2) on parsley seed germination (Petroselinum crispum) In Vitro. Biological Trace Element Research. 155: 283-286.(In Persian) (Journal)
He, J., Ren, Y., Chen, X. and Chen, H. 2014. Protective roles of nitric oxide on seed germination and seedling growth of rice (Oryza sativa L.) under cadmium stress. Ecotoxicology and environmental safety. 108: 114-119. (Journal)
Ibrahim, E. A. 2016. Seed priming to alleviate salinity stress in germinating seeds. Journal of Plant Physiology. 192: 38-46. (Journal)
Jaberzadeh, A., Moaveni, P., Moghadam, H. R. T. and Zahedi. H. 2013. Influence of bulk and nanoparticles titanium foliar application on some agronomic traits, seed gluten and starch contents of wheat subjected to water deficit stress. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 41: 201-207.(In Persian) (Journal)
Kafi, M., Nezami, A., Hoseyni, H. and Masoomi, A. 2009. Physiological effects of drought stress by polyethylene glycol on germination of lentil (Lens culinaris Medik L.) genotypes. Journal of Iranian Field Crop Research. 1: 69-79. (In Persian) (Journal)
Khan, M. A. and Ungar, I. A. 1984. The effect of salinity stress and temperature on the germination of polymorphic seeds and growth of atriplex triangularis willd. American Journal of Botany. 71: 481-489. (Journal)
Khan, M. A., Ungar, I. A. and Showalter, A. M. 2000. The effect of salinity on the growth, water status, and ion content of a leaf succulent perennial halophyte, Suaeda fruticosa (L.) Forssk. Journal of Arid Environments. 45: 73-84. (Journal)
Khot, L. R., Sankaran, S., Maja, J. M., Ehsani, R. and Schuster, E. W. 2012. Applications of nanomaterials in agricultural production and crop protection: A review. Crop Protection. 35: 64-70. (Journal)
 
Kiapour, H., Moaveni, P., Habibi, D. and Sani, B. 2015. Evaluation of the application of gibbrellic acid and titanium dioxide nanoparticles under drought stress on some traits of basil (Ocimum basilicum L.). International Journal of Agronomy and Agricultural Research. 6: 138-150. (Journal)
Lu, C. M., Zhang, C. Y., Wen, J. Q., Wu, G. R. and Tao, M. X. 2002. Research of the effect of nanometer materials on germination and growth enhancement of Glycine max and its mechanism. Soya Bean Science. 21: 168-172. (Journal)
Mahmoodzadeh, H. and Aghili, R. 2014. Effect on germination and early growth characteristics in wheat plants (Triticum aestivum L.) seeds exposed to TiO2 nanoparticles. Journal of Chemical Health Risks. 4: 29-36.  (In Persian) (Journal)
Mahmoodzadeh, H., Nabavi, M. and Kashefi, H. 2013. Effect of nanoscale titanium dioxide particles on the germination and growth of canola (Brassica napus). Journal of Ornamental and Horticultural Plants. 3: 25-32.‏(In Persian) (Journal)
Manai, J., Gouia, H. and Corpas, F. J. 2014. Redox and nitric oxide homeostasis are affected in tomato (Solanum lycopersicum L.) roots under salinity-induced oxidative stress. Journal of plant physiology. 171: 1028-1035. (Journal)
Misra, P., Shukla, P. K., Pramanik, K., Gautam, S. and Kole, C. 2016. Nanotechnology for crop improvement. Plant Nanotechnology. Springer International Publishing‏, pp: 219-256.
Mohammadi, R., Maali-Amiri, R. and Abbasi, A. 2013. Effect of TiO2 nanoparticles on chickpea response to cold stress. Biological Trace Element Research. 152: 403-410.(In Persian) (Journal)
Mohammadi,R., MaaliAmiri, R. and Mantri, N. L. 2014. Effect of TiO2 Nanoparticles on oxidative damage and antioxidant defense systems in Chickpea seedlings during cold stress. Russian Journal of Plant Physiology. 61: 768-775.(In Persian) (Journal)
Nakano, Y. and Asada, K. 1987. Purification of ascorbate peroxidase in spinach chloroplast: inactivation in ascorbate-depleted medium and reactivation by monodehydro ascorbate radical. Plant Cell Physiology. 28: 131-140. (Journal)
Nasibi, F., Kalantari, K. M. and Khodashenas, M. 2009. The effect of sodium nitroprusside (SNP) pretreatment on some biochemical parameters in tomato seedlings (Lycopersicun esculentum L.) under drought stress. Journal of Agricultural Sciences and Natural Resources. 16: 121-133.(In Persian) (Journal)
Nasir Khan, M. 2016. Nano-titanium dioxide (nano-Tio2) mitigates NaCl stress by enhancing antioxidative enzymes and accumulation of compatible solutes in tomato (Lycopersicon esculentum Mill.). Journal of Plant Science. 11: 1-11. (Journal)
Nazar, R., Iqbal, N., Syeed, S. and Khan, N. A. 2011. Salicylic acid alleviates decreases in photosynthesis under salt stress by enhancing nitrogen and sulfur assimilation and antioxidant metabolism differentially in two mungbean cultivars. Journal of plant physiology, 168: 807-815.
Pais, I. 1983. The biological importance of titanium. Journal of Plant Nutrition. 6: 3-131. (Journal)
Qazvini, H. A., Yousefi, A. and Islam majidi. H. 2002. Afzal barley, soutable for planting in salty lands in temperate region of country. Technical Publications of vice-presidency of agricultural extension, pp: 1-2. (In Persian)
Seabra, A. B., Rai, M. and Duran. N. 2015. Emerging role of nanocarriers in delivery of nitric oxide for sustainable agriculture. Nanotechnologies in Food and Agriculture. Springer International Publishing, pp: 183-207.
Sepehri, A. and Rouhi, H. R. 2016. Enhancement of Seed Vigor Performance in Aged Groundnut (Arachis hypogaea L.) Seeds by Sodium Nitroprusside under Drought Stress. Philippine Agricultural Scientist. 99: 339-347. (Journal)
Shalaby, E. E., Epstein, E. and Qualset, O. C. 1993. Variation in salt tolerance among some wheat and triticale genotypes. Journal of Agronomy and Crop Science. 171: 298-304. (Journal)
Siddiqui, M. H. and Al-Whaibi, M. H. 2014. Role of nano-SiO2 in germination of tomato (Lycopersicum esculentum). Saudi Journal of Biological Sciences. 21: 13-17.
Suzuki, N., Koussevitzky, S., Mittler, R. O. N. and Miller, G. A. D. 2012. ROS and redox signalling in the response of plants to abiotic stress. Plant, Cell and Environment. 35: 259-270. (Journal)
 
Tan, B. C., Chin, C. F. and Alderson, P. 2013. Effects of sodium nitroprusside on shoot multiplication and regeneration of Vanilla planifolia Andrews. In Vitro Cellular and Developmental Biology-Plant, 49: 626-630. (Journal)
Zhang, Y., Wang, L., Liu, Y., Zhang, Q., Wei, Q. and Zhang, W. 2006. Nitric oxide enhances salt tolerance in maize seedlings through increasing activities of proton-pump and Na+/H+ antiport in the tonoplast. Planta, 224: 545–555. (Journal)
Zheng, C., Jiang, D., Liu, F., Dai, T., Liu, W., Jing, Q. and Cao, W. 2009. Exogenous nitric oxide improves seed germination in wheat against mitochondrial oxidative damage induced by high salinity .Environmental and Experimental Botany, 67: 222-227. (Journal)
Zheng, L., Hong, F., Lu, S. and Liu, C. 2005. Effect of nano-TiO2 on strength of naturally aged seeds and growth of spinach. Biological Trace Element Research, 105: 83-91. (Journal)