بررسی تأثیر کاربرد باکتری‌های افزاینده رشد گیاه (PGPR) بر قابلیت جوانه‌زنی بذر، بنیه گیاهچه و برخی ویژگی‌های مرتبط دورگ‌های دیررس ذرت

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشیار پژوهش، مؤسسه تحقیقات ثبت و گواهی بذر و نهال، سازمان تحقیقات آموزش و ترویج کشاورزی، کرج، ایران

2 دانشیار پژوهش، مؤسسه تحقیقات خاک و آب کشور، سازمان تحقیقات آموزش و ترویج کشاورزی، کرج، ایران

3 استاد پژوهش، موسسه تحقیقات اصلاح و تهیه نهال و بذر، سازمان تحقیقات آموزش و ترویج کشاورزی، کرج، ایران

چکیده

به­منظور بررسی تأثیر کاربرد باکتری­های افزاینده رشد گیاه (PGPR) ازتوباکتر کروکوکوم، آزوسپیریلوم لیپوفروم و سودوموناس فلورسنس بر قابلیت جوانه­زنی و بنیه بذر و گیاهچه دورگ­های ساده دیررس ذرت700، 704 و یک دورگ امیدبخش B73×K18 پژوهشی در آزمایشگاه تجزیه کیفی بذر مؤسسه تحقیقات ثبت و گواهی بذر و نهال به اجرا در آمد. تیمارهای آزمایش شامل تلقیح بذرهای این دورگ­ها با تک تک باکتری­ها، به­صورت دوتایی، سه تایی و عدم تلقیح باکتریایی به­عنوان تیمار شاهد بودند. ویژگی­های مورد بررسی شامل درصد جوانه­زنی نهایی، متوسط جوانه­زنی، متوسط جوانه­زنی روزانه، ضریب سرعت جوانه­زنی، سرعت جوانه­زنی روزانه، طول گیاهچه، ریشه و ساقه اولیه، وزن خشک گیاهچه، ساقه و ریشه اولیه و شاخص بنیه گیاهچه بودند. نتایج بدست آمده مشخص ساخت که به­جز متوسط جوانه­زنی روزانه و ضریب سرعت جوانه­زنی سایر ویژگی­های مورد بررسی تحت تأثیر اثرمتقابل دورگ­ها و PGPR قرار گرفتند. همچنین مشخص گردید که کاربرد مایه تلقیح تلفیقی از این سه جنس باکتری سبب بیش­ترین افزایش در قابلیت جوانه­زنی بذر و ویژگی­های مرتبط با بنیه گیاهچه هر سه دورگ شده و به­ترتیب مایه تلقیح دارای دو باکتری ازتوباکتر کروکوکوم و سودوموناس فلورسنس و تلقیح بذر با تک تک این باکتری­ها از تأثیر بیش­تری برخوردار بودند. همچنین ویژگی­های مورد بررسی بذر دورگ SC704 بیش از دورگ­های دیگرتحت اثرات مثبت باکتری­های مورد مطالعه قرار گرفته و از این لحاظ دورگ­های B73×K18 و700 در مرتبه­های بعدی قرار داشتند. بررسی رابطه همبستگی بین ویژگی­های بررسی شده نیز وجود همبستگی بالای بین آن­ها را نشان داد. بنابراین، براساس نتایج حاصل از این پژوهش کاربرد PGPR تأثیر معنی­داری بر ارتقای کیفیت بذر و بهبود بنیه گیاهچه دو رگ­های ذرت مورد مطالعه داشتند.

کلیدواژه‌ها


عنوان مقاله [English]

Study on effect of Plant Growth Promoting Rhizobacteria (PGPR) application on seed germination ability, seedling vigor and some related characters of late maturity Maize (Zea mays L.) hybrids

نویسندگان [English]

  • Aidin Hamidi 1
  • Ahmad Asgharzadeh 2
  • Rajab Choukan 3
1 Research Associate Professor, Seed and Plant Certification and Registration Institute (SPCRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
2 Research Associate Professor, Soil and Water Research Institute (SWRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
3 Research Professor, Seed and Olant Improvement Institute (SPII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
چکیده [English]

In order to study the effect of Azotobacter chroococcum, Azospirillum lipoferum and Pseudomonas fluorescen application on late maturity Maize (Zea mays L.) single cross hybrids (SC700, SC 704 and a promising single cross, B73×K18) seed germination ability and seedling vigor a laboratory research was conducted. Experiment treatments including hybrids seeds were single inoculated with one by one bacteria and co inoculated by two and three bacterial combined inoculants and no inoculation as control treatment. Also studied characters including: final germination percent, mean time to germination, mean daily germination, coefficient of velocity of germination, daily germination speed, seedling, primary root and shoot length, seedling, primary root and shoot dry matter and seedling vigor index were determined. Results revealed that except of mean daily germination and coefficient of velocity of germination, other studied traits affected by interactions of hybrids and Plant Growth Promoting Rhizobacteria. Likewise, revealed that application of an inoculants of three bacteria combination have highest growth promoting effect on germination ability and traits related to seedling vigor of all hybrieds and Azotobacter chroococcum and Pseudomonas fluorescens and seed single inoculation by those bacteria  have most growth promoting effect respectively. Also, revealed that studied characters of SC704more than other hybrids affected by growth promoting effect of studied bacteria and respectively B73×K18 and SC700 affected by studied Plant Growth Promoting Rhizobacteria stimulating effect and studied characters have high correlation among them. Therefore, based on this research results application of studied Plant Growth Promoting Rhizobacteria have considerable effect on Maize studied hybrids seed seedling enhancement.

کلیدواژه‌ها [English]

  • mean germination time
  • Seed enhancement
  • Seed inoculation
  • Seedling weight vigor index

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