بررسی اثر اسید هیومیک بر جوانه‌زنی، رشد گیاهچه و رنگیزه‌های فتوسنتزی گیاه دارویی اسفرزه (Plantago ovata Forssk)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار، گروه مرتع و آبخیزداری، دانشگاه زابل

2 کارشناس ارشد مرتعداری، گروه مرتع و آبخیزداری، دانشگاه زابل

چکیده

به­منظور بررسی اثر اسیدهیومیک بر شاخص­های جوانه­زنی و رنگیزه­های فتوسنتزی گیاه دارویی اسفرزه آزمایشی در قالب طرح کامل تصادفی انجام شد. تیمارها شامل غلظت­های 0، 15 و 30 گرم در لیتر بود. نتایج نشان داد اسیدهیومیک بر سرعت جوانه­زنی و شاخص بنیه بذر تاثیر معنی­دار داشت. بیش­ترین و کم­ترین سرعت جوانه­زنی، بیش­ترین و کم­ترین شاخص بنیه بذر به­ترتیب در تیمار 30 گرم در لیتر و شاهد محاسبه گردید. درحالی­که اسید هیومیک بر درصد جوانه­زنی و میانگین زمان جوانه­زنی اثر معنی­دار نداشت. اسیدهیومیک بر طول و وزن خشک ریشه­چه (سطح 1 درصد)، طول و وزن خشک ساقه­چه و ضریب آلومتری (سطح 5 درصد) معنی­دار بود، ولی بر وزن تر ریشه­چه و ساقه­چه اثر معنی­داری نداشت. بیش­ترین و کم­ترین طول ریشه­چه، بیش­ترین و کم­ترین وزن خشک ریشه­چه، بیش­ترین و کم­ترین وزن خشک ساقه­چه به­ترتیب در تیمار 30 گرم در لیتر اسید هیومیک و شاهد به­دست آمد. بیش­ترین طول ساقه­چه مربوط به غلظت 15 گرم در لیتر و کم­ترین آن مربوط به تیمار شاهد بود. بیش­ترین ضریب آلومتری در تیمار 30 گرم لیتر حاصل شد. بهترین تأثیر بر رنگیزه­های کلروفیلی در تیمار 30 گرم بر لیتر مشاهده شد به­طوری­که بیش­ترین و کم­ترین کلروفیل a، b، کلروفیل کل و کاروتنوئید به­ترتیب در غلظت 0 و 30 گرم بر لیتر اسید هیومیک مشاهده گردید. به­طور کلی نتایج نشان داد اسیدهیومیک در غلظت 30 گرم بر لیتر بر بهبود کیفیت اسفرزه موثر می­باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Investigation Effect of Humic Acid on Germination, Seedling growth and photosynthesis pigments of Medicinal Plant Isabgol (Plantago ovata Forssk)

نویسندگان [English]

  • Mahdieh Ebrahimi 1
  • Elham Miri Karbasak 2
چکیده [English]

The study was conducted to investigate the effect of humic acid on germination indexes and photosynthesis pigments of medicinal plant Plantago ovata Forssk in a completely randomized design. Experimental treatments comprised the following dosage 0, 15, 30 g Li-1. Results relived that humic acid had significantly effect on germination rate and seed vigor index. The highest and the lowest germination rate, the highest and the lowest seed vigor index were measured in dose of 30 gLi-1 and the control treatment respectively, while humic acid was not effective on germination percentage and mean of germination time. Humic acid showed significant effect on radical length and dry weight (p<1%), length and pedicel dry weight, and allometric coefficient (p<5%), however seedlings were not different significantly in radical and pedical fresh weight in response to humic acid. The highest and the lowest radical length, the highest and the lowest radical dry weight, the highest and the lowest pedicel dry weight were measured in dose of 30 gLi-1 and the control treatment respectively. The highest pedicel length was related to dose of 15 gLi-1 and the lowest was observed in the control treatment. The highest allometric coefficient was obtained in 30 gLi-1 dosage. The best effect on chlorophyll pigments was obtained in dose of 30 gLi-1. The highest and lowest chlorophyll a, chlorophyll b and total chlorophyll were observed in 30 and 0 gLi-1 respectively. In general, results showed that humic acid in dose of 30 gLi-1 was effective to enhancing quality of P. ovata Forssk.

کلیدواژه‌ها [English]

  • Germination rate
  • humic acid
  • Medicinal plants
  • Seed vigor index
Arnon, D.I. 1949. Copper enzyme polyphenoloxides in isolated chloroplast in Beta vulgaris. Plant Physiology, 24: 1-15. (Journal)
Asenjo, M.C.G., Gonzalez, J.L. and Maldonado, J.M. 2000. Influence of Humic extracts on germination and growth of ryegrass. Communications in Soil Science and Plant Analysis, 31(2): 101-114. (Journal)
Azam, F. and Kauser, K.A. 1983. Effect of humic acid soaking on seedling growth of wheat (Triticum aestivum L.) under different conditions. Pakistan Journal of Botany, 15(1): 31-38. (Journal)
Behbodian, B., Lahouti, M. and Nezami, A. 2005. Effects evaluation of salt stress on germination of chickpea varieties. Agriculture, 28(2): 127-137. (In Persian)(Journal)
Blumenthal, M., Goldberg, A. and Brinckmann, J. 2000. Herbal Medicine: Expanded commission E monographs. Integrative Medicine Communication. USA. (Book)
Chen, Y. and Aviad, T. 1990. Effects of humic substances on plant growth. Soil Science Society of American Journal, 161-186. (Journal)
Cordeiro, F.C., Catarina, C.S., Silveira ,V. and De Souza, S.R. 2011. Humic acid effect on catalase activity and the generation of reactive oxygen species in corn (Zea mays). Bioscience, Biotechnology, and Biochemistry, 75(1): 70-74. (Journal)
Ellis, R.H. and Roberts, E.H. 1981. The quantification of ageing and survival in orthodox seeds. Seed Science and Technology,9(2): 377-409. (Journal)
Eyheraguibel, B., Silvestre, J. and Morard, P. 2008. Effects of humic substances derived from organic waste enhancement on the growth and mineral nutrition of maize. Bioresource Technology, 99(5): 10-25. (Journal)
Ferrara, G., Pacifico, A., Simeone, P. and Ferrara, E. 2008. Preliminary study on the effects of foliar applications of humic acids on ‘Italia’ table grape. Journal International des Sciences de la Vigne et du Vin, 42: 79-87. (Journal)
Ghasemi, A., Tavakalo, M.R. and Zabihi, H.R. 2012. Effect of nitrogen, potassium and humic acid on vegetative growth, nitrogen and potassium uptake of potato minituber in greenhouse condition. Journal of Agriculture and Plants Breeding, 8(1): 39-56. (In Persian)(Journal)
Griffe, P., Metha, S. and Shankar, D. 2003. Organic production of medicinal, aromatic and dye-yielding Plants (MADPs): Forward, Preface an Introduction. FAO, 32-43. (Handbook)
Gulser, F., Sonmez, F. and Boysan, S. 2010. Effects of calcium nitrate and humic acid on pepper seedling growth under saline condition. Journal of Environmental Biology, 31(5): 873-876. (Journal)
Jones, C.A., Jacobsen, J.S. and Mugaas, A. 2004. Effects of humic acid on phosphorus avaliability and spring wheat yield. Facts Fertilizer, 32-41. (Journal)
Kauser, A. and Azam. F. 1985. Effect of humic acid on wheat seeding growth. Journal Environmental and Experimental Botany, 25: 245-252. (Journal)
Lee, Y.S. and Bartlett, R.J. 1976. Stimulation of plant growth by Humic substances. Soil Science, 40: 876-879. (Journal)
Liu, C. and Cooper. R.J. 2000. Humic substances influence creeping bent grass growth. Golf Course Management, 49-53. (Journal)
Malik. K.A., Bhattin, A. and Kauser, F. 1979. Effect of soil salinity on the decomposition and humification of organic matter by cellulolytic fungi. Mycologia, 719: 811-820. (Journal)
Mereddy, R., Hallgren, Y. and Conway, K.E. 2000. Solid matrix priming improves seedling vigor Okra seeds. Proceedings of the Oklahoma Academy of Science, 80: 33-37. (Conference)
Michael, K. 2001. Oxidized lignites and extracts from oxidizwd lignites in agriculture. Soil Sciences,1-23. (Handbook)
Nardi, S., Pizzeghello, D., Muscolo, A. and Vianello, A. 2002. Physiological effects of humic substances on higher plants. Soil Biology and Biochemistry, 34: 1527-1536. (Journal)
Piccolo, A., Celanoand, G. and Pietramellara, G. 1993. Effects of fractions of coal-derived humic substances on seed germination and growth of seedlings (Lactuca sativa and Lycopersicon esculentum). Biology and Fertility of Soils, 16: 11-15. (Journal)
Ramandant, M.F. 2005. Nutritional value, functional properties and nutraceuticals applications of black cumin (Nigella sativa L.): an overview. International Journal of Food Science and Technology, 42: 1208-1218. (Journal)
Rauthan, B.S. and Schnitzer, M. 1981. Effects of a soil fulvic acid on the growth and nutrient content of cucumber (Cucumis sativus) plants. Plant Soil, 63: 491-495. (Journal)
Rubio, V., Bustos, R., Irigoyen, M.L., Cardona-Lopez, X., Rojas-Triana, M. and Paz-Ares, J. 2009. Plant hormones and nutrient signaling. Plant Molecular Biology, 69(4): 361-73. (Journal)
Sabzevari, S., Khazaie, H.R. and Kafi, M. 2009. Effect of humic acid on root and shoot growth of two wheat cultivars (Triticum aestivum L.). Journal of Water and Soil, 23 (2): 87-94. (In Persian) (Journal)
Safarnejad, A., Salami, M.R. and Hamidi, H. 2007. Morphological characterization of medicinal plants (Plantago ovata, Plantago psyllium) in response to salt stress. Pajouhesh and Sazandegi, 75: 152-160. (In Persian) (Journal)
Saravanakumar, D., Vijayakumar, C., Kumar, N. and Samiyappan, R. 2007. PGPR-induced defense responses in the tea plant against blister blight disease. Crop Protection, 26: 556-565. (Journal)
Shahsavan Markadeh, M. and Chamani, E. 2014. Effects of variations concentrations and time of humic acid application on quantitative characteristics of cut stock flower (Matthiola incana Hanza). Journal of Science and Technology of Greenhouse Culture, 19: 157-170. (In Persian)(Journal)
Shariff, M. 2002. Effect of lignitic coal derived HA on growth and yield of wheat and maize in alkaline soil. Ph.D Dissertation, NWFP Agriculture University Peshawar, Pakistan. (Thesis)
Tan, K.H. and Tantiwiramanond, F. 1983. Effect of Humic acids on nodulation and dry matter production of soybean, peanut and clover. Soil Science Society of American Journal, 47: 1121-1124. (Journal)
Tattini, M., Chiarini, A., Tafani, R, and Castagneto, M. 1990. Effect of humic acids on growth and nitrogen upyake of container grown Olive. (Olea europaea L.( ‘MAURINO’. Acta Horticulturae, 286: 125-128. (Journal)
Türkmen, Ö., Dursun, A., Turan, M. and Erdinç, Ç.  2004. Calcium and humic acid affect seed germination, growth, and nutrient content of tomato (Lycopersicon esculentum L.) seedlings under saline soil conditionspp. Acta Agriculture Scandinavica, 7: 168-174. (Journal)
Ulukan, H. 2008. Effect of soil applied humic acid at different sowing times on some yield colponents in wheat hybrids. International Journal of Botany, 4(3): 164-175. (Journal)
Verlinden, G.T., Coussens, A., Vliegher, D. and Baert, G. 2010. Effect of humic substances on nutrient uptake by herbage and on production and nutritive value of herbage from sown grass pastures. Grass and Forage Science, 65(1): 133-144. (Journal)
Wiese, A. and Larry Binning, K. 1987. Calculating the threshold temperature of development for weeds. Weed Science, 35: 177-179. (Journal)
Xuenyuan, G., Xiaorong, W., Zhimany, G., Lemei, D. and Yijun, C. 2001. Effect of hemic acid speciation and bioavailability to wheat of rare earth elements in soil. Chemical Speciation and Bioavailability,13(3): 83-88. (Journal)
Young, C.C. and Chen, Y. 1997. Polyamines in humic acid and their effect on radical growth of lettuce seedlings. Plant Soil, 195: 143-149. (Journal)
Zachariakis, M., Tzorakakis, E., Kritsotakis, I., Siminis, C.I. and Manios, V. 2001. Humic substances stimulate plant growth and nutrient accumulation in grapevine rootstocks. Acta Horticulturae, 549: 131-13. (Journal)