بررسی اثر عصاره ورمی‌کمپوست و تنش خشکی بر شاخص‌های جوانه‌زنی نخود (Cicer arietinum L.cv. Pirouz)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه زیست‌شناسی دانشکده علوم دانشگاه لرستان، خرم‌آباد، ایران

2 گروه زراعت و اصلاح نباتات دانشکده کشاورزی دانشگاه لرستان، خرم آباد، ایران

چکیده

عصاره کود ورمی­کمپوست به­دلیل ساختار متخلخل، ظرفیت نگه­داری آب بالا، تنظیم کننده­های رشد گیاهی و وجود مقادیر بالای عناصر غذایی پرمصرف و کم مصرف، می­تواند تأثیر تنش­های مختلف را بر گیاهان کاهش ­دهد. در این راستا      به­منظور بررسی برهم­کنش سطوح مختلف عصاره آبی ورمی­کمپوست و تنش خشکی بر شاخص­های جوانه­زنی نخود آزمایشی به­صورت فاکتوریل در قالب طرح کاملاً تصادفی با چهار تکرار در دانشگاه صنعتی خاتم­الانبیاء بهبهان در سال 1393 انجام شد. عوامل مورد بررسی شامل عصاره ورمی­کمپوست در چهار سطح (0، 5، 10 و 20 درصد حجمی) و تنش خشکی در چهار سطح (0، 3/0- ، 6/0- و 9/0- مگاپاسکال) بود. تنش خشکی توسط پلی­اتیلن­گلایکول 6000 اعمال شد. عصاره ورمی­کمپوست در تیمار خشکی 3/0- مگاپاسکال توانست برخی شاخص­های جوانه­زنی مورد بررسی را بهبود دهد، به­طوری­که سطح 10 درصد عصاره بیشترین میزان درصد جوانه­زنی (66/63 درصد)، طول ریشه­چه (31/4 سانتی­متر)، سطح 20 درصد بیشترین میزان وزن خشک ساقه­چه (6/50 میلی­گرم) و سطح 5 درصد بیشترین میزان وزن خشک ریشه­چه (89 میلی­گرم) را در مقایسه با سطح شاهد داشتند. در تیمارهای 6/0- و 9/0- مگاپاسکال، عصاره ورمی­کمپوست به جز وزن خشک ریشه­چه نتوانست کاهش شاخص­های جوانه­زنی را که در اثر تنش خشکی ایجاد شده بود، جبران کند. در حالت کلی پژوهش حاضر نشان داد که کاربرد عصاره ورمی­کمپوست در مرحله جوانه­زنی در سطوح بالای تنش خشکی تأثیر معنی­داری ندارد اما در تنش­های خشکی ملایم (3/0- مگاپاسکال) مؤثر است.

کلیدواژه‌ها


عنوان مقاله [English]

Interaction effects of vermicompost extract and drought stress on germination indices of chickpea (Cicer arietinum L.cv. Pirouz)

نویسندگان [English]

  • SaeedReza Hosseinzadeh 1
  • Hamzeh Amiri 1
  • Ahmad Ismaili 2
چکیده [English]

Vermicompost extract can reduce negetive effects of different stresses on plant, due to its porous structure, high water storage capacity, and exsistance of some substances resembling hormones and plant growth regulators. This study was performed to investigate interactions of different levels of vermicompost extract and drought stress on germination characteristics of chickpea seedlings. The experiment was conducted in completely randomized design with four replications was conducted in 2014 at the Khatam Alanbia University of Behbahan. Seeds were sown in Petri dishes at different concentrations of vermicompost extract (0, 5, 10 and 20%) and four levels of negative water potential (0, -0.3, -0.6 and -0.9 MPa) that was prepared by PEG 6000. The seeds were placed in the germinator under controlled conditions.Vermicompost extract at -0.3 MPa significantly improved some germination indices, so that 10% levels of extract had the highest germination percentage 63.66%), radicle length 4.31 cm), 20% levels had the highest plumule dry weight (50.6 mg) and 5% levels had the highest radicle dry weight (89 mg) compared to control. Under -0.6 and -0.9 MPa, vermicompost extract, did not compensate for the negative effects of drought stress on germination characteristics. In general, this study showed that the use vermicompost extract during seed germination no significant effect under Severe drought stress, but under moderate drought stress (-0.3 MPa) is effective.

کلیدواژه‌ها [English]

  • Consumed endosperm
  • germination percentage
  • Germination speed index
  • PEG
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