Evaluation of agronomic and biochemical properties of seed storage protein quality in different soybean genotypes [Glycine Max (L.) Merrill]

Document Type : Research Paper

Authors

1 Ph.D Candidate of Molecular Genetics and Genetics Engineering, Sari Agricultural Sciences and Natural Resources University (SANRU), Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari, Iran

2 Professor, Department of Plant Breeding and Biotechnology, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran

3 Professor, Department of Plant Breeding and Biotechnology, Sari Agricultural Sciences and Natural Resources University (SANRU), Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari, Iran

4 Assistant Professor, Department of Plant Breeding and Biotechnology, Sari Agricultural Sciences and Natural Resources University (SANRU), Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari, Iran

Abstract

Soybeans are considered as a main source of oil and protein production in the world. On the other hand, soybean seeds are one of the most important sources of protein that is directly or indirectly (in the livestock industry) in the human diet due to its favorable and inexpensive proteins. In this study some of agronomic characteristics (such as: plant height, number of branches, number of pods, seed weight, seed yield and day to maturity) and also seed storage protein quality properties (such as: soluble protein content, protease inhibitors contents and lectin protein) in six mutant lines and nine cultivars were studied. The results demonstrated that the 032-240-D-P1 and 032-240-D-P4 mutant lines have a high grain yield potential in addition to desirable agronomic traits. In terms of qualitative properties of grain proteins, Nekador and Caspian cultivars had the highest protein and the highest of albumin and globulin storage proteins, respectively. Also, the evaluation of anti-nutritional factors also revealed that all the mutant lines and Arian cultivar had the lowest protease inhibitors (PIA) and lectin (HAU) protein, which could be considered as promising genotypes for the soybean breeding programs. Results from this study suggested that the 032-240-D-P4 mutant line by high seed yield and the lowest of anti-nutritional factors could be studied as a new variety.

Keywords

References

Adams, M.R., Golden, D.L., Franke, A.A., Potter, S.M., Smith, H.S. and Anthony, M.S. 2004. Dietary soy β-conglycinin (7S globulin) inhibits atherosclerosis in mice. The Journal of Nutrition, 134(3): 511-516. (Journal)
Amiri, A.M., Faraji, A., Ajamnorozei, H. and Payghamzadeh, K. 2013. Evaluation of agronomical and phonological traits of soybean cultivars at different management systems in gorgan region. Plant Ecophysiology (Arsanjan Branch), 5(14): 74-85. (In Persian)(Journal)
Armour, J.C., Perera, R.L.C., Bucham, W.C. and Grant, G. 1998. Protease inhibitors and lectins in soya beans and effects of aqueous heat-treatment. Journal of Science and Food Agriculture, 78: 225–31. (Journal)
Becker-Ritt, A.B., Mulinari, F., Vasconcelos, I.M. and Carlini, C.R. 2004. Antinutritional and/or toxic factors in soybean (Glycine max (L) Merril) seeds: comparison of different cultivars adapted to the southern region of Brazil. Journal of the Science of Food and Agriculture, 84(3): 263-270. (Journal)
Bradford, M.M. 1976. A rapid and sensitive method for quantification of microgram quantities of protein of utilizing the principle dye binding. Analyz Biochemistry, 72: 680-685. (Journal)
Carrao-Panizzi, M.C., Kwanyuen, P., Erhan, S.Z. and Lopes, I.D.O.N. 2008. Genetic variation and environmental effects on beta-conglycinin and glycinin content in Brazilian soybean cultivars. Pesquisa Agropecuaria Brasileira, 43(9): 1105-1114. (Journal)
Ciabotti, S., Silva, A.C.B.B., Juhasz, A.C.P., Mendonca, C.D., Tavano, O.L., Mandarino, J.M.G. and Gonçalves, C.A.A. 2016. Chemical composition, protein profile, and isoflavones content in soybean genotypes with different seed coat colors. International Food Research Journal, 23(2): 621-629. (Journal)
Cober, E.R. and Voldeng, H.D. 2000. Developing high-protein, high yield soybean populations and lines. Crop Science, 40: 39-42. (Journal)
De Muelenaere, H.J.H. 1964. Effect of heat treatment on the haemagglutinating activity of legumes. Nature, 201: 1029-1030. (Journal)
Fasina, Y.O., Classen, H.L., Garlich, J.D., Swaisgood, H.E. and Clare, D.A. 2003. Investigating the possibility of monitoring lectin levels in commercial soybean meals intended for poultry feeding using steam-heated soybean meal as a model. Poultry Science, 82(4): 648-656. (Journal)
Fehr, W.R., Hoeck, J.A., Johnson, S.L., Murphy, P.A., Nott, J.D., Padilla, G.I. and Welke, G.A. 2003. Genotype and environment influence on protein components of soybean. Crop Science, 43:511–514. (Journal)
Friedman, M. and Brandon, D.L. 2001. Nutritional and health benefits of Soy proteins. Journal of Agriculture and Food Chemistry, 49: 1069-1086. (Journal)
 
Ghanbari, S., Nooshkam, A., Fakheri, B.A. and Mahdinezhad, N. 2018. Assessment of Yield and Yield Component of Soybean Genotypes (Glycine Max L.) in North of Khuzestan. Journal of Crop Science and Biotechnology, 21(5): 435-441. (Journal)
Gu, C., Pan, H., Sun, Z. and Qin, G. 2010. Effect of soybean variety on anti-nutritional factors content and growth performance and nutrients metabolism in rat. International Journal of Molecular Sciences, 11: 1048-1056. (Journal)
Hymowitz, T. and Kaizuma, N. 2008. Dissemination of soybeans (Glycine max): Seed protein electrophoresis profiles among Japanese cultivars. Econimic Botany, 10(1007): 311-319. (Journal)
Iqbal, S., Mahmood, T., Tahira, M., Ali, M., Anwar, M. and Sarwar, M. 2003. Path coefficient analysis in different genotypes of soybean (Glycine max (L) Merril). Pakistan Journal of Biological Sciences, 6: 1085-1087. (Journal)
Kitamura, K. 1995. Genetic improvement of nutrition and food processing quality in soybean. Japan Agricultural Research Quarterly, 29: 1-8. (Journal)
Krishnan, H.B., Kim, W., Jang, S. and Kerley, M.S. 2009. All three subunits of soybean β-Conglycinin are potential food allergens. Journal of Agriculture and Food Chemistry, 57: 938-943. (Journal)
Krishnan, H.B., Natarajan, S.S, Mahmoud, A.A. and Nelson, R.L. 2007. Identification of glycinin and β-conglycinin subunits that contribute to the increased protein content of high-protein soybean lines. Journal of Agricultural and Food Chemistry, 55(5): 1839-1845. (Journal)
Lajalo, F.M. and Genevese, M. 2002. Nutritional significance of lectins and enzyme inhibitors from legumes. Journal of Agriculture and Food Chemistry, 50: 6592–6598. (Journal)
Lee, H.S., Shin, Y.W., Kim, J.G. and Park, Y.H. 2004. Evaluation of protein dispersibility index as an indicator for soybean meal protein quality in growing pigs: I. Metabolic study. Journal of Dairy Science, 87: 174-174. (Journal)
Liener, I.E. 1995. Possible adverse effects of soybean anticarcinogens. Journal of Nutrition, 125: 744-750. (Journal)
Liener, I.E. 1996. Effects of processing on antinutritional factors in legumes: the soybean case. Archivos Latinoamericanos de Nutricion, 44: 48S–54S. (Journal)
Liener, I.E. and Kakade, M.L. 1980. Protease Inhibitors. In: Liener, .IE. (ed) Toxic constituents of plant food stuff. Academic Press, New York, pp 7–71. (BOOK)
Lin, P., Ye, X. and Ng, T.B. 2008. Purification of melibiose-binding lectins from two cultivars of Chinese black soybeans. Acta Biochimica Biophysica Sinica, 40(12):1029-1038. (Journal)
Liu, K. 2000. Expanding soybean food utilization. Journal of Food Technology, 54(7): 46–58. (Journal)
Livingstone, D., Beilinson, V., Kalyaeva, M., Schmidt, M.A., Herman, E.M. and Nielsen, N.C. 2007. Reduction of protease inhibitor activity by expression of a mutant Bowman-Birk gene in soybean seed. Plant Molecular Biology, 64(4): 397-408. (Journal)
Malik, M.F., Ashraf, M.U., Qureshi, A.S. and Khan, M.R. 2011. Investigation and comparison of some morphological traits of the soybean populations using cluster analysis. Pakistan Journal of Botany, 43(2): 1249-55. (Journal)
Mandal, S., Sahana, N., Rajarani, A.P. and Santha, I.M. 2013. Molecular cloning, characterization and expression of lipoxygenase 2 (lox-2) isozyme from Indian soybean [Glycine max (L.) Merrill] cv. Pusa 16. Indian Journal of Biochemistry and Biophysics, 50(1): 54-63. (Journal)
Norton, G. 1991. Proteinase inhibitors. In: D’Mello, F.J.P., Duffus, C.M., Duffus, J.H. (eds) Toxic substances in crop plants. The royal society of chemistry, Cambridge UK, pp 68-106. (Book)
Novak–Hajosa, M., Szamosb, J., Gasztonyib, M. and Hajosb, G.Y. 2010. Characterization of 2-propanol soluble seed proteins in mutant soybean (Glycine max) lines. Acta Alimentaria, 39(2): 164–168. (Journal)
Pesic, M., Vucelic-Radovic, B., Barac, M., Stojanovic, S. and Nedovic, V. 2007. Influence of different genotypes on trypsin inhibitor levels and activity in soybeans. Sensors, 7: 67-74. (Journal)
Sano, K. and Ogawa, H. 2014. Hemagglutination (inhibition) assay. Methods in Molecular Biology, 1200: 47–52. (Journal)
 
Schmidt, M.A., Hymowitz, T. and Herman, E.M. 2015. Breeding and characterization of soybean triple null; a stack of recessive alleles of Kunitz trypsin inhibitor, soybean agglutinin, and P34 allergen nulls. Plant Breeding, 134(3): 310-315. (Journal)
Schulze, H., Saini, H.S., Huisman, J., Hessing, M., Van Der Berg, W. and Verstegen, M.W.A. 1995. Increased nitrogen secretion by inclusion of soya lectin in the diets of pigs. Journal of the Science of Food and Agriculture, 69: 501–510. (Journal) 
Sharma, S., Kaur, M., Goyal, R. and Gill, B.S. 2014. Physical characteristics and nutritional composition of some new soybean (Glycine max (L.) Merrill) genotypes. Journal of Food Science and Technology, 51(3): 551–557. (Journal)
Silva, M.S., Naves, M.M.V., Oliveira, R.B.D. and Leite, O.D.S. 2006. Chemical composition and protein value of the soybean residue in relation to the soybean grain. Food Science and Technology, 26(3): 571-576. (Journal)
Taski-Ajdukovic, K., Djordjevic, V., Vidic, M. and Vujakovic, M. 2010. Subunit composition of seed storage proteins in high-protein soybean genotypes. Pesquisa Agropecuaria Brasileira, 45: 721-729. (Journal)
Yasothai, R. 2016. Antinutritional factors in soybean meal and its deactivation. International Journal of Science, Environment and Technology, 5(6): 3793-3797. (Journal)
Zarkadas, C.G., Gagnon, C., Gleddie, S., Khanizadeh, S., Cober, E.R. and Guillemette, R.J.D. 2007. Assessment of the protein quality of fourteen soybean [Glycine max (L.) Merr.] cultivars using amino acid analysis and two-dimensional electrophoresis. Food Research International, 40: 129‑146. (Journal)
Zarkadas, C.G., Voldeng, H.D., Yu, Z.R. and Choi, V. 1999. Assessment of the protein quality of nine northern adapted yellow and brown seed coated soybean cultivars by amino acid analysis. Journal of Agricultural and Food Chemistry, 47: 5009-5018. (Journal)   
Zilic, S., Sredojevic. S., Bozovic, I. and Jovanovic, M. 1998. Effects of the Kunitz trypsin inhibitor content on grain yield, total protein and oil content in soybean seed. Savremena poljoprivreda, 3(4): 67-70. (Journal)
Iranian Journal of Seed Sciences and Research
Volume 7, Issue 3
October 2020
Pages 391-404

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