Effect of priming on seed germination indices and enzyme activity of Alnus subcordata under light and dark conditions

Document Type : Research Paper

Authors

1 BSc. Student of Horticultural Sciences, Faculty of Crop Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

2 Assistant Professor, Department of Horticultural Sciences, Faculty of Crop Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

Abstract

Alder trees as ornamental tree in northern forests of Iran are most used in urban green space. However, its propagation and seedling production is not done in Iran. One of the easy methods of plant propagation is the use of seed. Seed priming technique can be used to improve seed germination. This experiment was conducted in order to study the effects of priming (without priming, potassium nitrate (0.02 and 0.04%, gibberellic acid 50 and 100 mg L-1, water) and Photoperiod (dark and bright) on germination and enzymatic activity of Alnus subcordata. The experiment was carried out in factorial arrangement based on completely randomized design. According to the results, the highest percentage of germination (53%) was observed in seeds primed with 100 mg L-1 gibberellic acid in darkness. The effect of priming on the activity of catalase enzyme was significant at 1% probability level. The maximum peroxidase activity was in the dark and in the treatment of 100 mg L-1 of gibberellic acid.The most activity of this enzyme was observed in seeds primed with gibberellic acid 100 mg L-1. The maximum length of stems in dark and light treatments was 3.8 and 2.7 cm, respectively, at 50 mg L-1 gibberellic acid treatment. The maximum vigor index was related to gibberellic acid treatment. According to the results, the use of gibberellic acid treatment and dark conditions are recommended to improve the characteristics of germination in alder seed.

Keywords

References

Alaey, M., Naderi, R., Khalighi, A. and Salami, R. 2005. Effect of different factors on seed germination of Persian cyclamen (Cyclamen persicum mill.). Research and Construction, 67: 36-43. (In Persian)(Journal)
Al-Absi, K.M. 2010. The effects of different pre-sowing seed treatments on breaking dormancy of mahaleb cherries seeds, Prunus mahaleb L. Seed Sciences and Technology, 38, 332-340. (Journal)
Ahmadloo, F., Tabari Kouchaksaraei, M., Azadi, P. and Hamidi, A. 2017. Improving Germination of Hawthorn (Crataegus pseudoheterophylla Pojark.) Seed by Potassium Nitrate, Sulfuric Acid and Stratification. Journal of Plant Research, 29 (2): 254-263. (In Persian)(Journal)
Amooaghaie, R. 2006. The Effect of Light, Cold Duration and Seed Age on Seed Germination of Ferula ovina. Journal of Science and Technology of Agriculture and Natural Resources, 10 (3) :289-298. (In Persian)(Journal)
Archin, Sh., Rahimian Mashhadi, H., Oveisi, R. and Tavacol Afshari, R. 2013. Germination of two Rumex Species in Response to Light and Soil Moisture Conditions, 27 (1): 111- 117. (Journal)
Ananeiva, D.H., Rusterucci, C., Holt, B.F. Dietrich, R.A., Parker, J.E. and Dangl, J.L. 2002. Runaway cell death, but not basal disease resistance, in Isd1 is SA- and NIM1/NPR1-dependent. Plant Journal, 29: 381–391. (Journal)
Bewley, J.D., Bradford, K.J., Hilhorst, H.W.M. and Nonogaki, H. 2013. In Seeds: Physiology of Development, Germination and Dormancy. Springer. Chapter, 6:287-288. (Book)
Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248-254. (Journal)
Burgers, E., McCu, P., Kwon, Y. and Shetty, K. 2007. Effect of vitamin C and folic acid on seed vigour response and phenolic-linked antioxidation activity. Bioresource Technology, 98: 1393-1404. (Journal)
 
De Atrip, N. and O’Reilly, C. 2007. Germination response of alder and birch seeds to applied gibberellic acid and priming treatments in combination with chilling. Annals and Forest Science, 64 (4): 385-394. (Journal)
Finch-Savage W.E. and Footitt, S. 2012. To germinate or not to germinate: a question of dormancy relief not germination stimulation. Seed Science Research, 22:243–248. (Book)
Flores, J., Gonzalez-Salvatierra, C. and Jurado, E. 2016. Effect of light on seed germination and seedling shape of succulent species from Mexico. Journal of Plant Ecology, 9 (2). 174-179. (Journal)
Forouzesh-Sotgavaberi, R., Ahmadi, M.T., Etemad, V. and Saeidi, H.R. 2009. Investigation on quantitative and qualitative characteristics of 19-years old plantation of Caucasian alder (Alnus subcordata) in Siahkal region. Iranian Journal of Forest, 10 (3): 267-278. (In Persian)(Journal)
Goggin, D.E., Powles, S.B., Toorop, P.E. and Steadman, K.J. 2011. Dark mediated dormancy release in stratified Lolium rigidum seeds is associated with higher activities of cell wall modifying enzymes and an apparent increase in gibberellin sensitivity. Journal of Plant Physiology, 168:527–533. (Journal)
Hae, I.A., Hoon Seob, S., Li Na, C., Hyeon Gil, J. and Jung Dae, L. 2013. Effect of Priming and Seed Pellet Technique for Improved Germination and Growth in Fraxinus rhynchophylla and Alnus sibirica. Korean Journal of Medicinal Crop Science, 21(1): 7-19. (Journal)
Hamzeei, J., Shayan Fard, J. and Fotohi, K. 2012. Seed priming effect on some quantitative and qualitative characteristics of two sugar beet cultivars. Journal of Crop Production and Processing, 2 (6): 155-164. (In Persian)(Journal)
Hemeda, H.M. and Kelin. B.P. 1990. Effects of naturally occurring antioxidants on peroxidase activity of vegetables  extracts. Journal of Food Science, 55: 184-185. (Journal)
Jamil, M. and E.S. Rha. 2007. Gibberllic acid (GA3) enhances seed water uptake, germination and early seedling growth in sugar beet under salt stress. Pakistan Journal of Biological Sciences, 10: 654-658. (Journal)
Karimi, M. 2018. An introduction to widely used ornamental trees, shrubs and climbers in landscaping. Sari University of Agricultural Sciences and Natural Resources, (In Persian)(Book)
Karimi, M. and Varyani, M. 2016. Role of priming technique on germination characteristics of calendula (Calendula officinalis L.) seeds. Journal of Agricultural Sciences, 61(3): 215-226. (Journal)
Kato, M. and Shimizu, S. 1987. Chlorophyll  metabolism in higher  plants. VII. Chlorophyll degradation  in senescing tobacco leaves: phenolic-dependent peroxidative degradation.  Canadian Journal of Botany, 65, 729-735. (Journal)
Khatami, S.R., Sedghi, M. and Seyed Sharifi, R. 2018. Study on the effect of priming on the activity of antioxidant enzymes in hybrid maize single cross 704 under drought stress. Iranian Journal of Seed Science and Technology, 2(6): 185-198. (In Persian)(Journal)
Martin, R.C., Pluskota W.E. and Nonogaki, H. 2010. Interaction of ABA and GA metabolism. In: Pua EC, Davey MR (eds) Plant Developmental Biology: Biotechnological Perspectives. Springer, Heidelberg, 383–404. (Book)
Moosavi, A., Tavakkol-Afshari, R., Sharif-Zadeh, F. and Aynehband, A. 2009. Effect of seed priming on germination characteristics, polyphenol oxidase, and peroxidase activities of four amaranth cultivars. Journal Food Agriculture and Environment,7: 353-358. (In Persian)(Journal)
Naglreiter, C., Reichenauer, T.G., Goodman, B.A. and Bolhar-Nordonkampf, H.R. 2005. Free radical generation in Pinus sylvestris and Larix deciduas seeds primed with polyethylene glycol or potassium salt solution. Plant Physiology and Biotechnology, 43(2): 117-123. (In Persian)(Journal)
Shayanmehr, F., Hosseinzadeh Colagar, A., Zare, H. and Yousefzadeh, H. 2014. Morphological variations of genus Alnus in Iran: assessment of five new taxa. Taxon. Biosystem, 6(18):45–64. (In Persian)(Journal)
Shayanmehr, F., Jalali, S., Hosseinzadeh Colagar, A., Yousefzadeh, H. and Zare, H. 2015. Pollen Morphology of the genus Alnus Mill. in Hyrcanian Forests, North of Iran. Applied Ecology and Environmental Research, 13(3):833–847. (Journal)
 
Iranian Journal of Seed Sciences and Research
Volume 7, Issue 3
October 2020
Pages 351-360

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