Identification of informative microsatellite alleles for control genes of germination components in rice under normal conditions and drought conditions

Document Type : Research Paper

Authors

1 MSc. Student, Department of Plant Production, Collage of Agricultural Sciences and Natural Resource, Gonbad Kavous University, Gonbad Kavous, Iran

2 Associate Professor, Department of Plant Production, Collage of Agricultural Sciences and Natural Resource, Gonbad Kavous University, Gonbad Kavous, Iran

3 Assistant Professor, Department of Plant Production, Collage of Agricultural Sciences and Natural Resource, Gonbad Kavous University, Gonbad Kavous, Iran

4 Instructor, Department of Basic Sciences and Technology Engineering, University of Gonbad Kavous, Gonbad Kavos, Iran

Abstract

Rice is the world's most important agricultural product after wheat and important role in feeding more than half of the world's population. The germination stage is one of the most important stages in the development of rice. Drought stress has a significant effect on the germination characteristics of rice. Identification of tolerant genotypes and genetic structure of control their traits are an important factor for tolerance in rice. In order to detection of informative SSR alleles for control genes of germination components in rice under normal conditions and drought conditions, an experiment was conducted using 102 genotypes of rice in two replications in a Completely Randomized Design in Laboratory of Gonbad Kavous University in 2018. Germination percentage, plumule length, radicle length, coleoptile length, number of radicle, total fresh weight of plumule and fresh weight of radicle were measured. Significant differences were detected between genotypes for germination traits in normal and drought stress conditions. The results of the association analysis at normal condition showed that RM12091A and RM1029B were associated with germination percentage, coleoptile length, radicle total weight and under drought stress condition, the RM129D was related to number of radicle and total weight of plumule and the RM530B allele were correlated with length of the coleoptile and the number of roots. The results of this study showed that genetic variation among rice genotypes and association analysis could be used to increase drought tolerance in rice cultivars.

Keywords


Abdolshahi, R., Omid, M., Talei, A.R. and Yazdi-Samadi, B. 2010. Evaluation of bread wheat genotypes for drought tolerance. ESci Journal of Crop Production, 3(1): 159-171. (Journal)

An, Z.W., Xie, L.L., Cheng H., Zhou, Y., Zhang, Q. and He, X.G. 2009. A silver staining procedure for nucleic acids in polyacrylamide gels without fixation and pretreatment. Analytical Biochemistry, 391(1): 77-9. (Journal)
Bal, A.R. and Chattopadhyay, N.C. 1984. Effect of NaCl and PEG 6000 on Germination and SeedlingGrowth of Rice (Oryza sativa L.). Biologia Plantarum, 27(1): 65-69. (Journal)
Cavusoglu, K. and Kabar, K. 2010. Effects of hydrogen peroxide on the germination and early seedling growth of barley under NaCl and high temperature stresses. Journal of Biology Science, 4: 70-79. (Journal)
Choi W.Y., Kangm S.Y. and Park, H.K. 2000. Effects of Water Stress by PEG on Growth and Physiological Traits in Rice Seedlings, Korean Journal of Crop Science, 45: 112 -117. (Journal)
Diwan, J.M., Channbyregowda, V., Shenoy, P., Salimath, B. and Hat, R. 2013. Molecular mapping of early vigor related QTLs in rice. Journal of Biology, 1: 24-30. (Journal)
Ebrahimi, A., Naghavi, M.R., Sabokdast, M. and Moradi, A.S. 2011. Association analysis of agronomic traits with microsatellite markers in Iranian barley landraces barley. Modern Genetics Journal, 6: 35-43. (In Persian)(Journal)
Grishma Shah. H., Sasidharan, N., Chakraborty, S., Trivedi, R., Ravikiran, R. and Davla, D. 2012. Genetic diversity and molecular analysis for fertility restorer genes in rice (Oryza sativa. L.) for wild abortive (WA) cytoplasm using microsatellite markers. Journal of Agricultural Technology, 8 (1): 261-271. (Journal)
Jamal, I.H., Bari, A., Khan, S. and Zada, I. 2009. Genetic variation for yield and yield components inrice. Journal of Agricultural and Biological Science, 4: 60-64. (Journal)
Lasaltia, F., Miranda, J.G. and Michelle, I. 2008. Physiological characterization for drought tolerance of selected rice varieties in Lake Sebu, Philippines. USM R and D Journal, 16:13-16. (Journal)
Li, C., Miao, H., Wei, L., Zhang, T., Han, X. and Zhang, H. 2014. Association Mapping of Seed Oil and Protein Content in Sesamum indicum L. Using SSR Markers. Plos One, 9: 1-12. (Journal)
Mardani, Z., Rabiei, B., Sabouri, H. and Sobouri, A. 2013 Mapping of QTLs of Germination Characteristics under Nonstress and Drought Stress in Rice. Rice Science, 20(6). 391-399. (Journal)
Michel, B.E. and Kaufman, M.R. 1973. The osmotic potential of polyethylene glycol 6000. Plant physiological, 51, 914–916. (Journal)
Ming, H., Fang-Min, X., Li-Yun, C.H., Xiang-Qian, Z.H., Jojee, L. and Madonna, D. 2010. Comparative analysis of genetic diversity and structure in rice using ILP and SSR markers. Rice Science, 17 (4): 257-268. (Journal)
Mohammad Alagh, Sh. and Sabouri, H. 2014. Locations of controlling QTLs for germination parameters under the manicure-induced smoke stress in rice, The 13th Iranian Conference on Plant Breeding and Agronomy and the 3rd Iranian Seminar on Science and Technology, Agrarian Sciences and Reforming Association Iran Plants, https://www.civilica.com/Paper-NABATAT13-NABATAT13_0832.html. (Conference)
Mohammadi, R., Naghavi, M.R. Maali Amiri, R. and Rezaei, M. 2010. Identification of microsatellite markers in Iranian alfalfa. Modern Genetics Journal, 5: 7-66 (In Persian)(Journal)
Mohammadi-Nejad, G., Singh, R.K., Arzani, A., Rezaie, A.M., Sabouri, H. and Gregorio, G.B. 2010. Evaluation of salinity tolerance in rice genotypes. International Journal of Plant Production, 4 (3): 199-208. (Journal)
Naghavi, M.R., Mardi, M., Ramshini, H.A. and Fazelinasab, B. 2004. Comparative analyses of the genetic diversity among bread wheat genotypes based on RAPD and SSR markers. Iranian Journal of Biotechnology, 2: 195-202. (In Persian)(Journal)
Pirdashti, H., Tahmasebi Servestani, Z., Nematzade, Gh. and Abdolbaghi, A. 2001. Effects of drought stress at different growth stages of rice varieties. Abstracts 8th Iranian Crop Science Congress. Sep. 3-5. Gilan, Iran. (In Persian)(Conference)
Rabey, H., Salem, K.H. and Mattar, M. 2013. The genetic diversity and relatedness of eight rice (Oryza Sativa L.) cultivar as revealed by AFLP and SSRs marker. Life Science Journal, 10: 1471-1479. (Journal)
Sabouri, H., Biabani, A., Sabouri, A., and Mohammad Esmaili, M. 2010. The study of QTLs related to seed vigour under stress caused to Sorbitol in rice. Journal of Plant Production, 17: 123-136 (In Persian) (Journal)
Saghi Maroof, M.A., Biyaoshev, R.M., Yang, G.P., Zhang, Q. and Allard, R.W. 1994. Extra ordinarily polymorphic microsatellites DNA in barly species diversity, chromosomal location, and population dynamics. Processing of the academy of sciences, USA, 91: 4566-5570. Science, 6 (12): 355- 363. (Journal)
Sajib, A.M., Musharraf Hossain, M.D., Mosnaz, A.T.M.J., Hossain, H., Monirul Islam, M.D., Shamsher, A. and Prodhan, S.H. 2012. SSR marker-based molecular characterization and genetic diversity analysis of aromatic landreces of rice (Oryza sativa L.). Journal of Bioscience and Biotechnology, 1: 107-116. (Journal)
Sohani, M. 1996. Seed Control and Certification. Guilan university press. 166p. (In Persian) (Book)
Tang, S., Zhang, Y., Zeng, L., Luo, L., Zhong, Y. and Geng, Y. 2010. Assessment of genetic diversity and relationships of upland rice accessions from Southwest China using microsatellite markers. Official Journal of the Societa Botanica Italiana, 144 (1): 85-92. (Journal)
Tao, H., Brueck, H., Dittert, K., Kreye, C., Lin, S. and Sattelmacher, B. 2006. Growth and yield formation for rice (Oryza sativa L.) in the water-saving ground cover rice production system (GCRPS). Field Crops Research, 95: 1–12. (Journal)
Tuberosa, R., Gill, B.S. and Quarrie, S.A. 2002. Cereal genomics: Ushering in a brave new world. Plant Molecular Biology, 48 (5): 445-449. (Journal)
Wang, Z.J., Wang, Y.B., Wu, Y. and Zhang, H. 2011. Quantitative trait loci controlling rice seed germination under salt stress. Euphytica, 178, 297-307. (Journal)
Wen, W., Mei, H., Feng, F., Yu, S., Huang, Z., Wu, J., Chen, L., Xu, X. and Luo, L. 2009. Population structure and association mapping on chromosome 7 using a diverse panel of Chinese germplasm of rice (Oryza sativa L.). Theoretical and Applied Genetics, 119: 459-470. (Journal)
Yang, J.C., Liu, K., Zhang, S.F., Wang, X.M., Wang, Z.Q. and Liu, L.J. 2008. Hormones in rice spikelets in responses to water stress during meiosis. Acta Agronomica Sinica, 34: 111–118. (Journal)
Zheng, M., Tao, Y., Hussain, S., Jiang, Q., Peng, S., Huang, J., Cui, K. and Nie, L. 2016. Seed priming in dry direct-seeded rice: consequences for emergence, seedling growth and associated metabolic events under drought stress. Plant Growth Regulation, 78(2): 167-178. (Journal)
Zhou, J., You, A., Ma, Z., Zhu, L. and He, G. 2012. Association analysis of important agronomic traits in japonica rice germplasm. African Journal of Biotechnology, 11(12): 2957-2970. (Journal)