Comparison the effects of different of seed priming techniques on improving germination and antioxidant enzymes activity in borage seedlings

Document Type : Research Paper

Authors

1 Ph.D. Candidate of Agronomy, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran

2 Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran

Abstract

One of the problems of producing medicinal plants, such as borage, is unequal and poor germination and seedling establishment. Priming is one of the technique for improving seed germination and seedling growth in crops and medicinal plants. This research was carried out in order to study the effect of seed priming on germination, growth and biochemical and enzymatic characteristics of borage seedlings. The Experimental treatments were hydro-priming for 48 hours, seed priming with 0.85 mM ascorbic acid for 48 hours, 4 mM salicylic acid concentration for 60 hours, 20 mM potassium nitrate (KNO3) concentration for 24 hours and control. The results showed that seed priming were significantly increased the seed germination, growth and biochemical characteristic of borage seedlings. The mean germination time and the percentage of abnormal seedlings were significantly decreased by priming the borage seeds. The highest percentage and rate of germination, vigor index and length of seedlings were obtained from hydro-priming seeds, which were significantly higher than control. Also, hydro-priming decreased the abnormal seedlings about caused 80% and mean germination time about 25%. The activity of catalase and peroxidase enzymes and proline in seedlings from primed seeds were significantly higher than those of the control. The highest activity of catalase enzyme and proline content were observed in seed priming of 20 mM KNO3 concentration for 24 hours and 4 mM salicylic acid for 60 hours respectively, which did not have significant difference with hydro-priming. Hydro-priming, caused increase 2.7 fold in the activity of peroxidase enzymes seedlings. Generally, among the treatments, hydro-priming for 48 hours for improving germination, growth and biochemical characteristics of borage is considered as the best treatment.
 

Keywords


Aebi, H. 1984.Catalase in vitro. Method of Enzymology, 105: 121-126. (Journal)
Afzal, I., Aslam, N., Mahmood, F., Hameed, A., Irfan, S. and Ahmed, G. 2004. Enhancement of germination and emergence of canola seeds by different priming Techniques. Caderno de pesquisa Biology, 16(1): 19-34. (Journal)
Ahmadi, K., Shojaeian, A., Karimi, T. and Hajibarat, Z. 2018. Effect of priming with salicylic acid on germination indices on lallemantia medicinal seed (Lallemantia iberica) under drought stress. Journal of Seed Research, 8 (1): 78-87. (In Persian)(Journal)
Ahmadpour Dehkordi, S. and Balouchi, H.R. 2012. Effect of seed priming on antioxidant enzymes and lipids peroxidation of cell membrane in Black cumin (Nigella sativa) seedling under salinity and drought stress. Electronic Journal of Crop Production, 5(4): 63-85. (In Persian)(Journal)
Alivand, R., Tavakkol Afshari, R., Sharifzadeh, F. 2011. Effects of gibberellin, salicylic acid, and ascorbic acid on improvement of germination characteristics of deteriorated seeds of Brassica napus. Iranian Journal of Field Crop Science, 439: 561-571. (In Persian)(Journal)
Ansari, O., Azadi, M.S., Sharif-Zadeh, F. and Younesi, E. 2013. Effect of hormone priming on germination characteristics and enzyme activity of mountain rye (Secale montanum) seeds under drought stress conditions. Journal of Stress Physiology and Biochemistry, 9(3): 61-71. (Journal)
Artola, A., Carrillo-Castaneda, G. and Santos, G.G. 2003. Hydropriming: A strategy to increase Lotus corniculatus L. seed vigor. Seed Science and Technology, 31(2):455-463. (Journal)
Ashrafi, A. and Razmjou, K. 2010. Evaluation of hydropriming effect on safflower physiological and biochemical characteristics under drought stress. Journal of Crop Ecophysiology, 1(1): 34-44. (In Persian)(Journal)
Bahmani, M., Rahimi, D., Sadeghipour, A. and Kartuly Nezhad, D. 2016. Effects of priming with different concentrations of potassium salt on seed germination and vigor indices of (Capparis cartilaginea). Journal of Rangeland, 10(2): 180-190. (In Persian)(Journal)
Bates, L.S., Waldern, R.P. and Tear, I.D. 1973. Rapid determination of free proline for water stress studies. Plant and Soil, 39: 205-207. (Journal)
Batool, A., Ziaf, K. and Amjad, M. 2015. Effect of halo-priming on germination and vigor index of cabbage (Brassica oleracea var. capitata). Journal of Environmental and Agricultural Sciences, 2(7): 1-8. (Journal)
Caseiro, R., Bennett, M. and Marcos-Filho, J. 2004. Comparison of three priming techniques for onion seed lots differing in initial seed quality. Seed Science and Technology, 32: 365–375. (Journal)
Chance, B. and Maehly, A. 1955. Assay of catalases and peroxidase. Methods in Enzymolog, 2: 764-775. (Journal)
Chen, C. and Dickman, M.B. 2005. Proline suppresses apoptosis in the fungal pathogen Colletotrichum trifolii, Proceedings of National Academy of Seed, 102: 3459-3464. (Journal)
Demir Kaya, M., Okcu, G., Atak, M. and Kolsarici, O. 2006. Seed treatment to overcome salt and drought stress during germination in sunflower, European Journal of Agronomy, 24: 291-295. (Journal)
Donaldson, E., Schillinger, W. and Stephen, M. 2001 Straw production and grain yield relationships in winter wheat, Crop Science, 41: 100 106. (Journal)
Doulatabadian, A., Moddares Sanavy, S. and Etemadi, F. 2008. Effect of pretreatment of salicylic acid on wheat (Triticum aestivum L.) seed germination under salt stress, Iranian Journal of Biology, 4: 692-702. (In Persian)(Journal)
Ellis, R.H. and Roberts, E.H. 1981. The quantification of aging and survival in orthodox seeds. Seed Science and Technology, 9: 373-409.
Farhoudi. R., Saeedipour, S. and Mohammadreza, D. 2011. The effect of NaCl seed priming on salt tolerance, antioxidant enzyme activity, and proline and carbohydrate accumulation of Muskmelon (Cucumis melo L.) under saline condition. African Journal Agriculture Research, 6: 1363-1370. (Journal)
Farooq, M., Aziz, T., Basra, S., Cheema, M. and Rehman, H. 2008. Chilling tolerance in hybrid maize induced by seed priming with salicylic acid,Journal of Agronomy and Crop Science, 194: 161-168. (Journal)
Farooq, M., Basra, S., Warraich, A. and Khaliq, A. 2006. optimization of hydropriming Technigues for rice seed invigoration, seed science Technology, 34: 529-534. (Journal)
Fateh, H., Siosemardeh, A. and Karimpoorl, M. 2009. Effects of seed priming and sowing date on antioxidant enzymes activity and yield of chickpea under dryland condition. Plant Production Technology, 2: 1-16. (In Persian)(Journal)
Ganja, A., Ebadi, A., Jahanbakhsh, S. and Parmoon, G. 2017. Effect of salicylic acid priming on mobility of seed reserves and seed germination of wheat in comparison to wild rye in drought conditions, Journal of Seed Research, 7(3): 61-71. (In Persian)(Journal)
Ghassemi-Golezani, K. and Dalil, B. 2011. Seed Germination and Vigor Test. First Edition. Jahad Daneshgahi- Mashhad. (In Persian)(Book)
Harris, D., Pathan, A., Gothkar, P., Joshi, A., Chivasa, W. and Nyamudeza, P. 2001. Onfarm seed priming: using participatory methods to review and refine a key technology, Agriculture. Systems, 69: 151- 164. (Journal)
Hashemi, S., Asraf, Z. and Pourseyedi, S. 2010. Effects of seed pretreatment by salicylic acid on growth and some physiological and biochemical parameters in (Lepidium sativum), Journal of Plant Biology, 2(2): 1-10. (In Persian)(Journal)
Hayat, Q., Hayat, S., Irfan, M. and Ahmad, A. 2010 Effect of exogenous salicylic acid under Changing environment: a review, Environmental and Experimental Botany, 68: 14–25. (Journal)
International Seed Testing Association (ISTA). 2017. International Rules for Seed Testing. International Seed Testing Association, Bassersdorf, Switzerland. (Handbook)
Jokar Tangkarami, M., Ghanbari, A. and Moradi, F. 2016. The evaluation of salt stress effect on antioxidant enzymes activity in seedling induced of Milk thistle primed seeds, Journal of Seed Science and Research, 3(3): 11-21.(In Persian)(Journal)
Judy, M. and Sharifzadeh, F. 2006. Effect of priming in barley cultivars. Desert, 11 (1): 25-36. (In Persian)(Journal)
Khan, M.A. and Ungar, I. 2001. Seed germination of Triglochin maritime as influenced by salinity and dormancy relieving compounds, Journal of Biological Plant, 44: 301-307. (Journal)
Korkmaz, A. 2005. Inclusion of Acetyl Salicylic acid and Methyl Jasmonat the priming solution Improves Low-temperature Germination and Emergence of Sweet pepper, Hort science, 40(1): 197-200. (Journal)
Kuchaki, A., Shabahang, J., Khorram Dell, S. and Ghafouri, A. 2012, Ecological study of different patterns of cultivars of (Borage officinalis L.) and Beans (Phaseolus vulgaris L.), Journal of Agricultural Ecology, 4(1): 1-11.(In Persian)(Journal)
Madady, M. Khomari, S., Javadi, A. and Sofalian, A. 2016. The effect of priming with calcium nitrate and zinc oxide on seed germination and seedling growth of corncockle under salinity stress, Journal of Plant Process and Function, 5(15): 169-179.(In Persian)(Journal)
Massarat, N., Siadat, A., Sharafizadeh, M. and Habibi, B. 2013. The effect of priming on germination and growth of maize hybrid SC704 in drought and salinity stress condition, Journal of Plant Ecophysiology, 5(15):14-25.(In Persian)(Journal)
Misra, N. and Saxena, P. 2009 Effect of salicylic acid on proline metabolism in lentil grown under salinity stress, Plant Science, 177: 181–189. (Journal)
Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance, TrendsPlant Science, 7: 405-410. (Journal)
Moosavi, A., Tavakkol-Afshari, R., Sharif-Zadeh, F. and Aynehband, A. 2009. Effect of seed priming on germination characteristics, polyphenol oxidase, and peroxidase activities of four amaranth cultivars, Journal of Food Agriculture Environment, 7: 353- 358. (Journal)
Naghdi badi, H., Zeinali Mobarake, Z., Omidi, H. and Rezazade. S.H. 2012. Morphological, agronomical and phytochemical changes in borage (Borago officinalis L.) under biological and chemical fertilizers application, Journal of Medicinal Plants, 2(42): 145-156. (In Persian)(Journal)
Nair, A.S., Abraham, T. and Jaya, D. 2008. Studies on the changes in lipid peroxidation and antioxidants in drought stress induced cowpea (Vigna unguiculata L.) varieties. Journal of Environmental Biology, 29: 689-691. (Journal)
Neamatollahi, E., Bannayan, M., Souhani Darban, A. and Ghanbari, A. 2009. Hydropriming and Osmopriming Effects on Cumin (Cuminum Cyminum L.) Seeds Germination. World Academy of Science, Engineering and Technology, 3(9): 477-480. (Journal)
Nelson, C.P. 2000. Water potential. The key to successful seed priming. Decagon Devices, Inc. AN4101- 10. (Journal)
Netondo, G.W., Onyango, J. and Beck E. 2004. Sorghum and Salinity: I. Response of growth, water relation, and ion accumulation to NaCl salinity. Crop Science, 44:797-805. (Journal)
Norouzi Haroni, N. and Tabari Kouchsaraei, M. 2014. Effect of Hydropriming, Halopreming and Boiling Water on Seed Germination of black locust (Robinia pesudoacasia L.). Iranian Forests Ecology, 2 (3): 76-88.(In Persian)(Journal)
Omidi, H., Sorosh Zadeh, A., Salehi, A. and Ghezli, F. 2005. Evaluation osmo-priming pre-soaking on rapeseed seed germination, Agriculture Science Technology, 19: 125- 136. (In Persian)(Journal)
Paravar, A., Omidi, H., Sadat Esaynezhad, N. and Amirzad, M. 2015. Effect of hydroperimmuning on reducing the effects of salinity stress on germination and proline content of seeds of two species of Purple coneflower (Echinacea angustifolia) and chicory (Chicorium intybus), Journal of Seed Research, 5(4): 33-43.(In Persian)(Journal)
Parvin, M., Tajbakhsh, M. and Ghiyasi, M. 2017. Evaluation of the effect of potassium nitrate and seed hydroperimation pre-treatments on reducing the effects of salinity stress in germination and sesame seedling growth, The 2th National Conference Rainfed Medicinal Plants of Iran, in Urmia, Iran. PP: 372-375. (Conference)
Rabie, B. and Bayat, M. 2008. A study of seed germination and seedling growth indices of oilseed rape (Brassica napus L.) cultivars through seed vigour tests, Journal of Field Crop Science, 2: 93-104. (In Persian)(Journal)
Rwse, H., Mckee, J. and Finch-Savage, W. 2001. Membrane priming method for small samples of high value seeds, Seed Science and Technology, 29: 587-597. (Journal)
Safari Saatlu, M., Najat Zadeh, F. and Taghavi Tabat, R. 2015. The effect of priming on improving seed germination of Barley 21 cultivar with polyethylene glycol under salinity stress, New cellular and molecular biotechnology Journal, 6(21): 41-47. (In Persian)(Journal)
Sakhabutdinova, A., Fatkhutdinova, D., Bezrukova, M. and Shakirova, F. 2003. Salicylic acid prevents damaging action of stress factors on wheat plants, Bulg Journal of Plant Physiology, Special Issue: 314-319. (Journal)
Safari Saatlu, M., Najat Zadeh, F. and Taghavi Tabat, R. 2015. The effect of priming on improving seed germination of Barley 21 cultivar with polyethylene glycol under salinity stress, New cellular and molecular biotechnology Journal, 6(21): 41-47. (In Persian)(Journal)
Sakhabutdinova, A., Fatkhutdinova, D., Bezrukova, M. and Shakirova, F. 2003. Salicylic acid prevents damaging action of stress factors on wheat plants, Bulg Journal of Plant Physiology, Special Issue: 314-319. (Journal)
Salehi Sormagi, M. 2009. Medicinal Plants and Herbal. 3th edition, Publications nutrition (In Persian). (In Persian)(Book)
Sharifi Moghadam, H. and Ahmadi, Kh. 2017. The Effect of Potassium Nitrate Priming on the Properties of (Scrophularia Striata) under salinity stress, Journal of Seed Research, 7(3): 180-189. (In Persian)(Journal)
Sharma, A., Rathore, S., Srinivasan, K. and Tyagi, R. 2014. Comparison of various seed priming methods for seed germination, seedling vigour and fruit yield in okra (Abelmoschus esculentus L. Moench), Scientia Horticulturae, 165: 75-81. (Journal)
Shekari, F., Baljani, R., Afsahi, J., Kamran, S. and Shekari, F. 2009. Effect of seed priming with salicylic acid on growth characteristics of borage plants (Borago officinalis) seedlings, Agroecology Journal, 18: 47-53. (In Persian)(Journal)
Shekari, F., Baljani, R., Saba, J., Afsahi, K. and. Shekari, F. 2010. Effect of seed priming with salicylic acid on growth characterisics of borage (Borago officinalis) plants seedlings, Journal New Agricutuer Science, 6(18): 47-53. (In Persian)(Journal)
Unesi, E., Bahari, A., Azadi, M. and Ansari, O. 2012. The effect of priming and aging worn on the index millet seed germination and catalas in seed Panucum Miliaceum, Journal of Seed research, 3(4): 61-70. (In Persian)(Journal)
Varier, A., Kuriakose, A. and Dadlani, M. 2010. The subcellular basis of seed priming, Current Science, 99(4): 450-456. (Journal)