The influence of seed pre-treatment with Rhizobium and plant growth promoting rhizobacteria on nodulation and nitrogen fertilizer optimization in soybean (Glycine max L.)

Document Type : Research Paper

Author

Department of Agriculture and plant breeding, Astara branch, Islamic Azad University, Astara, Iran.

Abstract

In order to study the influence of soybean (Glycine max L.) seed pre-treatment with Rhizobium japanicum and plant growth promoting rhizobacteria (PGPR) on nodulation and nitrogen fertilizer optimization, a spilt plot experiment based on randomized complete block design with three replications was conducted in 2011 at the research farm of the Islamic Azad University, Ardabil Branch. Factors were: nitrogen fertilizer rates at four levels (0, 25, 50 and 75 kg urea/ha) in the main plot and soybean seed pre-treatment with Rhizobium and plant growth promoting rhizobacteria (PGPR) in four levels containing of without seed inoculation as control, inoculation seed with Rhizobium japanicum, Azotobacter chroococcum strain5, Azosprilium lipoferum strain OF. The results showed that with increasing the amount of fertilizer, fertilizer use efficiency was reduced. Maximum number (16) and dry weight (16 mg) of nodules was obtained in seed inoculation with Rhizobium japanicum along with application of 50 kg urea/ha and minimum of these traits were observed in without of urea fertilizer application and seed inoculation. So, the highest plant height (117.22 cm), number of pod per plant (54.72), grain hundred weight (28.26 g) and grain yield per area (267 g/m2) was obtained in Rhizobium japanicum whit application of 75 kg urea/ha. At the level of 75 kg urea/ha fertilizer, inoculation with Azosprilium, Azotobacter and Rhizobium bacteria increased seed yield (14.4, 21.4 and 40.6 percent, respectively) compared to non-inoculation. Based on the results, it was concluded that application of 75 kg urea/ha in seed inoculation with Rhizobium can be recommended for profitable soybean production in the study area.

Keywords

References

Abtahi, S.M., Seyed Sharifi, R. and Qaderi, F.2014. Influence of Nitrogen Fertilizer Rates and Seed Inoculation with Plant Growth Promoting Rhizobacteria (PGPR) on Yield, Fertilizer Use Efficiency, Rate and Effective Grain Filling Period of Soybean (Glycine max L.) in Second Cropping. Agriculture science and sustainable Production. 24(3): 111-129. (In Persian) (Journal)
Achakzai, A.K.K., and   Bangulzai, M.I. 2006. Effect of various levels of nitrogen fertilizer on the yield and yield attributes of pea (Pisum sativum L.) cultivars. Pakistan Journal of Botany. 32 (2): 331-340. (Journal)
Ahmed, R., Solaiman, A.R.M., Halder, N.K., Siddiky, M.A, and Islam, M.S. 2007. Effect of inoculation methods of Rhizobium on yield attributes, yield and protein content in seed of pea. Journal of Soil and Nature. 1(3): 30-35. (Journal)
Albayrak, S., Sevimay, C.S. and Tongel, O. 2006. Effect of inoculation with Rhizobium on seed yield and yield components of common vetch (Vicia sativa L.). Turkish Journal of Agriculture and Forestry. 30: 31-37.  (Journal)
Amany, A.B. 2007. Effect of plant density and urea foliar application on yield and yield components of chickpea (Cicer arietinum L.). Research Journal of Agriculture and Biological Sciences. 3 (4): 220-223. (Journal)
Cakmakci, R., Donmez, M.F. and Erdogan, U. 2007. The Effect of plant growth promoting rhizobacteria on barley seedling growth, nutrient uptake, some soil properties, and bacterial counts. Turkish Journal of Agriculture and Forestry. 31: 189-199.(Journal)
Caliskan, S., Ozkaya, I., Caliskan, M.E. and Arslan, M. 2008. The effect of nitrogen and iron fertilization on growth, yield and fertilizer use efficiency of soybean in Mediterranean type soil. Field Crops Research. 108:126-123. (Journal)
Cattelan, A.J., Hartel, P.G. and Fuhrmann, J.J. 1999. Screening for plant growth-promoting rhizobacteria to promote early soybean growth. Soil Science Society of America Journal .63 1670–1680. (Journal)
Chandrasekar, B.R., Ambrose, G. and Jayabalan, N. 2005. Influence of biofertilizers and nitrogen source level on the growth and yield of Echinochloa frumentacea (Roxb) Link. Journal of Agricultural Technology. 1: 2. 223-234. (Journal)
Chen, J. 2006. The combined use of chemical and organic fertilizers and or biofertilizer for crop growth and soil fertility, International Workshop on Sustained management of the Soil-Rhizospher System for Efficient Crop Production and Fertilizer use, October, 16-20, Thailand, pp.11. (Conference)
Dabaghian, Z., H. Pirdashti, A. Abasian and Bahari Saravi, S. H., 2015. The effect of biofertilizers, Thiobacillus, Azotobacter, Azospirillum and organic sulfur on nodulation process and yield of soybean (Glycine Max L. Merr.). Agronomy Journal (Pajouhesh & Sazandegi). 107: 17-25. (In Persian) (Journal)
Dileep Kumar, S.B., Berggren, I. and Martensson, A.M. 2001. Potential for improving pea production by inoculation with Fluorescent Pseudomonas and Rhizobium. Plant and Soil. 229: 25-34. (Journal)
Egamberdiyeva, D. 2007. The effect of plant growth promoting bacteria on growth and nutrient uptake of maize in two different soils. Applied Soil Ecology. 36:184-189. (Journal)
Fageria, N.K. and Baligar, V.C. 2005. Enhancing nitrogen use efficiency in crop plants. Advances in Agronomy. 88: 97-185. (Journal)
Goodroad, L.   and Jellum, M.D. 1988. Effect of N fertilizer rate and soil pH on N efficiency in corn. Plant and Soil. 106:  85- 89.(Journal)
Kader, M.K., Mmian, H. and Hoyue, M.S. 2002. Effects of Aztobacter inoculants on the yield and nitrogen uptake by wheat. Journal of Biological Sciences. 2(4): 250 –261. (Journal)
Kennedy, I.R., Choudhury, A.T.M.A. and Kecskes, M.L. 2004. Non-symbiotic bacterial diazotrophs in crop-farming systems: can their potential for plant growth promotion be better exploited? Soil Biology and Biochemistry. 36: 1229-1244. (Journal)
Mirzakhani, M., Ghanbari Kashan, M. and Farid Hashemi, S.A. 2017. Response of nitrogen physiological efficiency of safflower to animal and chemical fertilizers in Kashan region. Journal of Iranian Plant Ecophysiological Research (plant sciences research). 11(41): 53-64. (In Persian) (Journal)
Namvar, A., Seyed Sharifi, R., Sedghi, M., Asghari Zakaria, R., Khandan, T. and Eskandarpour, B. 2011. Study on the Effects of Organic and Inorganic Nitrogen Fertilizer on Yield, Yield Components, and Nodulation State of Chickpea (Cicer arietinum L.). Communications in Soil Science and PlantAnalysis. 42(9): 1097-1109. (Journal)
Ogutcu, H.,  Algur, O.F., Elkoca, E. and Kantar, F. 2008. The determination of symbiotic effectiveness of Rhizobium strains isolated from wild chickpea collected from high altitudes in Erzurum. Turkish Journal of Agriculture and Forestry. 32: 241-248. (Journal)
Premachandra, D., Hudek L. and Brau L. 2016. Bacterial Modes of Action for Enhancing of Plant Growth. Journal of Biotechnology and Biomaterials. 6(3):1-8. (Journal)
Rudresh, D.L., Shivaprakash, M.K. and Prasad, R.D. 2005. Effect of combined application of Rhizobium, phosphate solubilizing bacterium and Trichoderma spp. on growth, nutrient uptake and yield of chickpea (Cicer aritenium L.). Applied Soil Ecology. 28: 139-146. (Journal)
Salantur, A., Ozturk, A. and Akten, S. 2006. Growth and yield response of spring wheat (Triticum aestivum L.) to inoculation with rhizobacteria. Plant, Soil and Environment. 52 (3):111–118.(Journal)
Saleh-Rastin, N. 2002. Biofertilizers and their roles in sustainable agriculture.In "Necessity for the Production of Biofertilizers in Iran".Edited by: Khavazi, K and M. J. Malakouti. Agric. Education Press. Tehran. (Book) (In Persian).
Sanchez, E., Rivero, R.M., Ruzi, J.M. and Romero, L. 2004. Yield and biosynthesis of nitrogenous compounds in fruits of green bean (Phaseolus vulgaris L.C.V. strike) in response to increasing N fertilization. Journal of Science and Food Agriculture, 84: 575-580. (Journal)
Seiedi, M. and Seyed Sharifi, R. 2014. The Effects of Seed Inoculation with Rhizobium and Nitrogen Application on Yield and some Agronomi Characterstics of Soybean (Glycine max L.) under Ardabil Condition. Iranian Journal of Field Crops Research, 11(4), 618-628. (In Persian) (Journal)
Seyed Sharifi, R. 2010. Industrial Plants. (Second Edition). University of Mohaghegh Ardabili and Amidi Tabriz Press, Tabriz, Iran. (Book) (In Persian).
Seyed Sharifi, R., Zare Zad, Z., Barmaki1, M., Abdi, A. and Seyed Sharifi, R. 2016. Evaluation of nitrogen fertilizer application and seed inoculation by Azotobacter and Azosprillium on yield, rate and grain filling period of corn SC-500 cultivarIranian Journal of Seed Science and Research. 3 (1):1-15. (In Persian) (Journal)
Sharma, A.K. 2003. Biofertilizers for sustainable agriculture. Arobios, India. 30: 31-37. (Book)
 
Shrivastava, U.K., Rajput, R.L. and Dwivedi, M.L. 2000. Response of soybean-mustard cropping system to sulfur and bio-fertilizers on farmer’s field. Legume Research. 23: 277-278.(Journal)
Singh, G. 2005. Biofertilizer an eco-friendly fertilizer for the reclamation of over burden dum., National Biofertilizer Development Center, Govt, of India, Ministry of Agriculture. (Book)
Sogut, T. 2006. Rhizobium inoculation improves yield and nitrogen accumulation in soybean (Glycine max) cultivars better than fertilizer. New Zealand Journal of Crop and Horticultural Science. 34: 115-120.  (Journal)
Taghizadeh, R. and Seyed Sharifi, R. 2011. Effect of nitrogen fertilizer on yield attributes and nitrogen use efficiency in corn cultivars. Journal of Science and Technology of Agriculture and Natural Resources, Water and Soil Science. 15(57): 209-218. (In Persian) (Journal)
Vederia, L.B., Pastorino, N. and Ballati, A. 2001. Imcompatibility may not be the rule in the Sinorhizobium feredi-soybean interaction. Soil Biology and Biochemistry.33:837-840.(Journal)
Werner, D. and Newton, W.E. 2005. Nitrogen fixation in agriculture, forestry, ecology and environment. Published by Springer. pp: 347. (Book)
Wu, S.C., Cao, Z.H., Li, Z.G. and Cheung, K.C. 2005. Effect of biofertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth: A greenhouse trial. Geoderma.125: 155-166. (Journal)
Zahir, A.Z., Arshad, M. and Frankenberger, W.F.(Jr.). 2004. Plant growth promoting rhizobacteria: applications and perspectives in agriculture. Advances in Agronomy. 81:97-168. (Journal)
Zaidi, A. and Mohammad, S. 2006. Co-inoculation effects of phosphate solubilizing micro-organisms and Glomus fasciculatum on green gram-bradyrhizobium symbiosis. Turkish Journal of Agriculture and Forestry. 30: 223-230. (Journal)
Iranian Journal of Seed Sciences and Research
Volume 5, Issue 3
October 2018
Pages 117-129

Files

Share

How to cite

Statistics