Effect of seed priming on germination parameters, electrical conductivity, potassium leakage, and some seedling characteristics of pinto bean (Phaseolus vulgaris Var. Khomeyn) aged seed

Document Type : Research Paper

Authors

1 Dept. of Agronomy, MSc. Student in Agronomy, Faculty of Agriculture, Lorestan University

2 Agrotechnology Department, Lorestan University

3 Dept. of Soil Science, Faculty of Agriculture, Lorestan University

4 Dept. of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University

Abstract

Seed aging reduces seed quality, germination, and resulted seedling. This experiment was conducted with the aim of investigating possibility of mitigating the harmful effects of seed aging in pinto bean as a factorial experiment based on a completely randomized design with three replications in 2016 in faculty of agriculture, Lorestan University. Factors were included seed aging in two levels (aged and non-aged), and priming in five levels (control, hydropriming, gibberellin 100 ppm, salicylic acid 14 ppm, gibberellin 100 and salicylic acid 14 ppm). The results showed that germination percentage and rate, seed vigor index and germination uniformity decreased by seed aging, but the electrical conductivity, potassium leakage, calcium leakage and time to 50% of germination increased. Seed priming, especially hormonal priming improved seed quality. Gibberellin 100 ppm was the most effective treatment in reducing potassium leakage and increasing germination speed.

Keywords


Agrawal, R.L. 2004. Seed technology. Oxford and IH publishing Co. LTD. New Delhi.
Aliabadi Farahani, H. and Maroufi, K. 2011. Hydropriming and NaCl influences on seedling
growth in fenugreek (Trigonella foenum-graecum). Advances in Environmental Biology. 5(5): 821-827. ISSN 1995-0756.  (Journal)
Amanpour Balaji, B., Sedghi, M. and Khumari, S. 2011. Effect of rapid burnout on germination of bean seeds. Second National Conference on Seed Science and Technology, Islamic Azad University, Mashhad Branch. 257-253. (In Persian) (Journal)
Ansari, O., Tavakkol Afshari, R., Sharif-Zadeh, F. and Shayanfar, A. 2013a. The role of priming on seed reserve utilization and germination of mountain rye (Secale montanum) seeds under salinity stress. Iranian Journal of Field Crop Science. 44 (2): 189-181. (In Persian) (Journal)
Ansari, O., Azadi, M.S., Sharif-Zadeh, F. and Younesi, E. 2013b. Effect of hormone priming on
germination characteristics and enzyme activity of mountain rye (Secale montanum) seeds under drought stress conditions. Journal of Stress Physiology and Biochemistry. 9(3): 61-71. (Journal)
Ansari, O., Choghazardi, H.R., Sharif-Zadeh, F. and Nazarli, H. 2012. Seed reserve utilization and
seedling growth of treated seeds of mountain rye (Secale montanum) as affected by drought
stress. Cercetări Agronomice în Moldovia. 45(2): 43-48. (Journal)
Azarnia, H., Safikhani, S., Biabani, A., Gholam-Alipour, I. and Eisvand, H.R. 2013. Effect of seed pre-treatment on germination characteristics and physiological quality of lentil seed and seedling. International Conference on New Findings in Agricultural Sciences, Natural Resources and the Environment, 9-1. (Conference)
Azarnia, M. and Eisvand, H.R. 2013. Priming a method for improving seed quality for growth and performance of crops. Research Findings in Crops and Gardens. 2(4), 287-277. (In Persian) (Journal)
Azarnia, M., Safi Khani, S., Biabani, A., Gholam Ali Pour Alamdari, A. and Eisvand. H.R. 2013. Study of seed priming effects on some vegetative properties of lentil seedling. International Conference on New Findings in Agricultural Sciences, Natural Resources and the Environment. 9-1.
Basra, S.M.A., Farooq, M., Wahid, A. and Khan, M.B. 2006. Rice seed invigoration by hormonal
and vitamin priming. Seed Science and Technology. 34(3): 753-758. (Journal)
Best, B. 2007. Mechanism of aging.www.benbest.com/lifeext/aging.html.
Bewley, J. D., Bradford, K. J., Hilhorst, H.W.M. and Nonogaki, H. 2013. Seed physiology of development, ermination and dormancy. 3rd Edition. Springer New York, 392 pages. (Book)
Borji, M. and Sarlak, M. 2007. Some seed traits and their relationship to seed germination, emergence rate electrical conductivity in common bean (Phaseolus vulgaris L.). Asian Journal of Plant Science. 6(5): 781-787.
Eisvand, H. R., Azarnia, M., Nazarian, F. and Sharafi. R. 2011 a. Effect of gibberellin and absisic acid on some physiological characteristics of seed and seedling emergence of chickpea in rainfed
and irrigated conditions. Journal of Crop Production. 42(4):797-789. (In Persian) (Journal)
Eisvand, H.R. and Arefi, H.M. 2007. Effect of some plant growth regulators on physiological quality of Bromus inermis aged seeds. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research. 15(2): 171-159. (In Persian) (Journal)
Eisvand, H.R., Shahrosvand, S., Zahedi, B., Heidari, S. and Afroughe, S. 2011 b. Effects of
hydro‐priming and hormonal priming by gibberellin and salicylic acid on seed and seedling quality
of carrot (Daucus carota var. sativus). Iranian Journal of Plant Physiology. 1 (4): 233‐239. (Journal)
Eisvand, H.R., Dousti, A., Majnoun Hosseini, N. and Pour Babaie, A. 2014. Effects of PGPR bacteria and seed ageing on improving common bean (Phaseolus vulgaris L.) yield and yield components. Iranian Journal of field Crop Science. 45(2): 277-285. (Journal)
Eisvand, H. R., Azarnia, M. and Nazarian Firouzabadi, F. 2012. Effects of priming by gibberellin and abcsisic acid on emergence and some physiological characters of chickpea (Cicer arietinum L.) seedling under dry and irrigated conditions. Iranian Journal of Field Crop Science. 42(4): 789-797. (Journal)
Farajollahi, Z. and Eisvand, H.R. 2016. Storage duration and temperature of hydroprimed seeds affect some growth indices and yield of wheat. Iranian Journal of Plant Physiology. 7(1): 1909-1918.(In Persian) (Journal)
Emam, Y., Shekoofa, A., Salehi, F. and Jalali, A.H. 2010. Effects of viability and vigour of seed on establishment and grain yield if two common bean cultivars with contrasting growth habits. American-Eurasian Journal of Agriculture & Environmental Science. 9(5), 495-499. (In Persian)  (Journal)
Gharib, F.A. and Hejazi, A. Z. 2010. Salicylic acid ameliorates germination, seedling growth, phytohormone and enzymes activity in bean (Phaseolus vulgaris L.) under cold stress. Journal of American Science. 6(10): 675-683.(In Persian) (Journal)
Ghassemi-Golezani, K., Khomari, S., Dalil, B., Hosseinzadeh-Mahootchy, A. and Chadordooz-Jeddi, A. 2010. Effects of seed aging on field performance of winter oilseed rape. Journal of Food Agriculture and Environment. 8(1): 175-178. (Journal)
Hamedi, A. and Bakhtiari, S. 2011. Effect of seed priming on germination parameter of Phaseolus vulgaris L. under salinity stress. The Second National Conference on Seed Science and Technology, Islamic Azad University, Mashhad Branch. 1-6. (In Persian) (Journal)
Hosseini, F. 2008. Effect of seed burning on germination, establishment and yield of soybeans in Ahwaz climate. MSc. Thesis. Ahwaz University. 258 pages. (In Persian)
ISTA. 2016. International Rules for Seed Testing, Vol. 2016, Full Issues i-19-8(284). http://doi.org/10.15258/istarules. 2016.
Kapoor, N., Arya, A., Siddiqui, M.A., Amir, A. and Kumar, H. 2010. Seed deterioration in chickpea (Cicer arietinum L.) under accelerated ageing. Asian Journal of Plant Sciences. 9(3): 158-162.
Majnoon Hoseini, N. 2008. Grain Legume Production. Jihad-Daneshghahi Pub. University of Tehran.
283 pages. (In Persian)
Mehrabian Moghadam, N., Arvine, M.J., Khajavy Nejad, Gh.R. and Maghsoudi, K. 2011. Effect of
salicylic acid on growth and forage and grain yield of maize under drought stress in field conditions. Seed and Plant Production Journal. 27(2): 41-55. (In Persian) (Journal)
Mohammadi, H. 2013. The role of priming on seed reserve utilization and germination of barley
(Hordeum vulgare L.) seeds under drought stress. International Journal of Agronomy and Plant
Production. 4(10): 2543-2547. (In Persian) (Journal)
Mohammdi, H., Soltani, A., Sadeghipour, H.R. and Zeinali, E. 2011. Effects of seed aging on
subsequent seed reserve utilization and seedling growth in soybean. International journal of plant
Production. 5(1): 65-70. (In Persian) (Journal)
Mortazavi, S. M, Pasban Islam, B., Tajbakhsh, M. and Zardashti, R. 2005. Effect of seed deterioration and salinity on seed vigor of chickpea genotypes under laboratory and greenhouse conditions. Agricultural Knowledge. 15(2): 147-131. (In Persian) (Journal)
Mousavi Nik, S.M., Gholami Tilebeni, H., Zeinali, E. and Tavassoli A. 2011. Effects of seed ageing on heterotrophic seedling growth in cotton. America-Eurasian Journal of Agriculture and Environmental Science. 10(4): 653-657. (In Persian) (Journal)
 
Rehman, H., Farooq, M., Basra, S.M.A. and Afzal, I. 2011. Hormonal priming with salicylic acid improves the emergence and early growth in cucumber. Journal of Agriculture and Social Sciences. 7: 109-113. (Journal)
Roohi, A., Tajbakhsh, M., Bernoosi, A., Sa'idi, M. R. and Nikzad, P. 2011. Investigation of different pre-treatments effects on seed germination and seedling traits of various chickpea (Cicer arietinum L.) cultivars. Agronomy Journal (Pajouhesh & Sazandegi). 90: 1-8. (In Persian) (Journal)
Sharikova, F., Sakhabutdinova, A., Bezrukova, M., Fatkhutdinova, R. and Fatkhudinova, D. 2003.
Changes in the hormonal status of wheat seedling induced by salicylic acid and salinity. Plant
Science. 164(3): 317-322. (Journal)
Shekari, F. and Esfandiari, A. 2010. Physiology in Crop Production. University of Maragheh Press. p.
42-43. (In Persian) (Journal)
Sheykhbaglou, R., Rahimzadeh, S., Ansari, O. and Sedghi, M. 2014. The effect of salicylic acid and gibberellin on seed reserve utilization, germination and enzyme activity of Sorghum (Sorghum bicolor L.) seeds under drought stress. Journal of Stress Physiology and Biochemistry.10(1): 5-13.
Soltani, A. and Maddah, V. 2010. Simple, Applied Programs for Education and Research in Agronomy. ISSA press.
Soltani, B, Kamkar, A., Galeshi, S. and Ghaderi, A. 2009. Effect of seed deterioration on wheat emergence in response to environmental stress. Electronic Journal of Crop Production. 2(2): 43-57. (In Persian) (Journal)
Soltani, A., Gholipoor, M. and Zeinali, E. 2006. Seed reserve utilization and seedling growth of wheat as affected by drought and salinity. Environmental and Experimental Botany. 55: 195- 200. (In Persian) (Journal)
Tabatabai, A. and Ansari, A. 2016. Effect of copper sulfate stress and growth regulators on germination indices and biochemical changes of canola seed (Brassica napus L.). Iranian Journal of Seed Research. 1: 110-120. (In Persian) (Journal)
Tavili, A., Saberi, M., Shahriari, A. and Heydari, M. 2012. Salicylic acid effect on Bromus tomentellus germination and initial growth properties under cadmium stress. Journal of Plant Research. 26(2): 208- 216. (In Persian) (Journal)
 
Vishwanath, K., Pallavi, H. M., Devraju, P. J. and Prashanth, Y. 2011. Prediction of storability of different seed size grades of French bean varieties through accelerated ageing response. Research. Journal of Agricultural Science. 2(2): 213-216. (Journal)
Windauer, L., Altuna, A. and Benech-Arnold, R. 2007. Hydrotime analysis of Lesquerella fendleri seed germination responses to priming treatments. Industrial Crops and Products. 25(1): 70-74. (Journal)