Effect of nano-zinc oxide and drought stress on the activity of hydrolytic enzymes and seed reserves mobilization of soybean (Glycine max. L) cultivar Katul (DPX)

Document Type : Research Paper

Authors

1 Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Iran

2 university of tabriz

Abstract

To evaluate the effect of drought stress originated from poly ethylene glycol and application of nano ZnO on the activity of hydrolytic enzymes and soybean seed reserves mobilization a factorial experiment was conducted based on completely randomized design with three replications at the University of Mohaghegh Ardabili in 2016. Treatments were drought stress at three levels as 0, -0.7 and -1.4 MPa and nano zinc oxide at the concentrations of 0, 0.75 and 1.5 g lit-1. Germination percentage and rate, seedling length and dry weight, activity of hydrolytic enzymes (alpha-amylase, protease and lipase) and seed reserves (carbohydrate, protein and lipid) mobilization were measured. Results showed that nano zinc oxide had the positive effect on germination percentage and rate, activity of hydrolytic enzymes and mobilization of carbohydrate, lipid and protein reserves. So that application of 1.5 g lit-1 of nano ZnO increased the activity of lipase, protease and α-amylase about 11.75, 12.75 and 11.87%, respectively in severe drought stress compared to non-stress condition. Drought stress also decreased the seed reserves mobilization in addition to reducing the activity of hydrolytic enzymes. There was a reduction trend in the mobilization of carbohydrates, lipids and proteins under drought stress with the increasing in imbibition time, while application of nano ZnO increased the mobilization of seed reserves which is the indicator of growth and production of higher seedling dry weight. Considering the ameliorative effect of nano ZnO on the damages due to drought stress and increasing the seed reserves using efficiency, activity of hydrolytic enzymes and germination percentage it seems that application of nano ZnO in the concentration of 1.5 g lit-1 will improve the soybean seed germination under drought conditions.

Keywords


Alyari, H., Shekari, F. and Shekari, F. 2000. Oilseeds. Cultivation and Physiology. Amidi Publications. Tabriz, Iran, 182p.  (Book)
Ashraf, M. and Vahid, S. 2000. Time–course changes in organic metabolites and mineral nutrients in germinating maize seeds under salt (NaCl) stress. Seed Science and Technology, 28(3): 641-656. (In Persian)(Journal)
Bagci, S. A., Ekiz, H., Yilmaz, A. and Cakmak, I. 2007. Effect of Zn deficiency and drought on grain yield of field grown wheat cultivars in Central Anatolia. Journal of Agronomy and Crop Science, 193: 198–206. (Journal)
Bennett, J. P. and Skoog, F. 2002. Preliminary experiments on the relation of growth-promoting substances to the rest period in fruit trees. Plant Physiology, 13(2): 219–225. (Journal)
Bradford, K. J. and Nonogaki, H. 2007. Seed development, dormancy and germination. Blackwell Publishing Ltd. 389pp. (Book)
Brown, P. H., Cakmak, I. and Zhang, Q. 1993. Form and function of zinc in plants, In: Robson, A. D. (Ed.). Pp: 93-106. (Book)
Cakmak, I. 2008. Enrichment of cereal grains with zinc: Agronomic or genetic biofortification. Plant and Soil, 30(2): 1-17. (Journal)
Cakmak, I. and Marschner, H. 1985. Mechanism of phosphorous induced zinc deficiency in cotton. I. zinc deficiency enhanced uptake rate of phosphorous. Physilogiae Plantareum, 68: 483-490. (Journal)
Cakmak, I., Kalayci, M., Brauni, H. J., Kilinc, Y. and Yilmaz, A. 1999. Zn deficiency as a practical problem in plant and human nutrition in Turkey: A Nato-Science for stability project. Field Crop Research, 60: 175-188. (Journal)
Cui, Y. Y., Pandey, D. M., Hahn, E. J. and Paek, K. Y. 2004. Effect of drought on physiological aspects of Crassulacean acid metabolism in Doritaenopsis. Plant Science, 167: 1219-1226. (Journal)
Dodd, G. L. and Donovan, L. A. 1999. Water potential and ionic effects on germination and seedling growth of two cold desert shrubs. American Journal of Botany, 86:1146-153. (Journal)
Doman, D. C., Walker, J. C., Trelease, R. N. and Moore, B. D. 1982. Metabolism of carbohydrate and lipid reserves in germinated cotton seeds. Planta, 155(6): 502-510. (Journal)
Ellis, R. H. and Roberts, E. H. 1981. The quantification of aging and survival in orthodox seeds. Seed Science and Technology, 9: 373-409. (Journal)
Graham, R. D., Alscher, J. S. and Haynes, S. C. 1992. Selecting Zinc-efficient cereals genotypes for soils of low Zn status. Plant and Soil, 146: 241-250. (Journal)
Gubler, F., Millar, A. A. and Jacobsen, J. V. 2005. Dormancy release, ABA and pre-harvest sprouting. Current Opinion in Plant Biology. 8: 183–187. (Journal)
Gupta, V. K. and Gupta, S. P. 1984. Effect of zinc sources and levels on the growth and Zn nutrition of soybean (Glycine max L.) in the presence of chloride and sulphate salinity. Plant and Soil, 81: 299–304. (Journal)
Hajiboland, R. 2012. Effect of micronutrient deficiencies on plant stress responses. In: Abiotic Stress Responses in Plants (eds. Ahmad, P. and Prasad, M. N. V) pp. 283-329. Springer, USA. (Book)
Hajiboland, R. and Amirazad, F. 2010. Drought tolerance in Zn-deficient red cabbage (Brassica oleracea L. var. capitata f. rubra) plants. Horticultural Sciences, 37: 88–98. (Journal)
Holwerda, B. C. and Rogers, J. C. 1992.Purification and characterization of aleurain. Plant Physiology, 99:848-855. (Journal)
Huang, A. H. C. 1985. Lipid bodies, in: Linskins HF & Jackson F (eds). Modern Methods of Plant Analysis. Berlin: Springer Verlag. 145-151. (Book)
Iraki, S. N., Bressan, R. A. and Carpita, N. C. 1989. Cell walls of tobacco cells and changes in composition associated with reduced growth upon adaptation to water and slain stress. Plant Physiology, 91: 48-53. (Journal)
Irigoyen, J. J., Emerich, D. W. and Sanchez-Diaz, M. 1992. Water stress induced changes in concentration of proline and total soluble sugars in nodulated alfalfa plants. Physiologia Plantarum, 84: 55-60. (Journal)
 
Jacobsen, D. W., Pearce, A., Poole, T., Pharis, R. P. and Mander, L. N. 2002. Abscisic acid, phaseic acid and gibberellin contents associated with dormancy and germination in barley. Physiologia Plantarum,115: 428–441. (Journal)
Kaur, S., Gupta, A. K. and Kaur, N. 1998. Gibberellic acid and kinetin partially reverse the effect of water stress on germination and seedling growth. Plant Growth Regulation, 25: 29-33. (Journal)
Kaya, M. D., Okcu, G., Atak, M., Cıkılı, Y. and Kolsarıcı, O. 2006. Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). European Journal of Agronomy, 24: 291-295. (Journal)
Khan, N. A., Nazar, R., Iqbal, N. and Anjum, N. A. 2012. Phytohormones and abiotic stress tolerance in plants. Springer Heidelberg Dordrecht London New York. 311pp. (Book)
Kim, Y. C., Nakajima, M., Nakayama, A. and Yamaguchi, I. 2005. Contribution of gibberellins to the formation of Arabidopsis seed coat through starch degradation. Plant and Cell Physiology. 46: 1317–1325. (Journal)
Klee, H. and Estelle, M. 1991. Molecular genetic approaches to plant hormone biology. Annual Review in Plant Physiology and Plant Molecular Biology, 42: 529–551. (Journal)
Kwak, J. M., Mori, I. C. and Pei, Z. M.2003. NADPH oxidase AtrbohD and AtrbohF genes function in ROS-dependent ABA signaling in Arabidopsis. The EMBO Journal, 22: 2623–2633. (Journal)
Ling, D. and Xing, B. 2007. Phytotoxicity of nanoparticles: Inhibition of seed germination and root growth. Environmental Pollution, 150: 243-250. (Journal)
Liu, Y., Schiff, M., Czymmek, K., Tallo´ czy, Z., Levine, B. and Dinesh-Kumar, S. P. 2005. Autophagy regulates programmed cell death during the plant innate immune response. Cell, 121: 567–577. (Journal)
Lopez-Millan, A. F., Ellis, D. R. and Grusak, A. 2005. Effect of zinc and manganese supply on the activities of superoxide dismutase and carbonic anhydrase in Medicago truncatula wild type and raz mutant plants. Plant Science, 168: 1015–1022. (Journal)
Marschner, H. 1995. Mineral nutrition of higher plants. 2nd Academic Press. Ltd. London. pp 889. (Book)
Michel, B. E. and Kaufmann, M. R. 1973. The osmotic potential of polyethylene glycol 6000. Plant Physiology, 51: 914-916. (Journal)
Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science, 7: 405–409. (Journal)
Mousavi Nik, S. M., Gholami Tilebeni, H., Zeinali, E. and Tavassoli A. 2011. Effects of seed ageing on heterotrophic seedling growth in Cotton. Emerica-Eurasian Journal of Agriculture and Environmental Sciences, 10(4): 653-657. (Journal)
Muscolo, A., Sidari, M., Anastasi, U., Santonoceto, C. and Maggio, A. 2014. Effect of PEG-induced drought stress on seed germination of four lentil genotypes. Journal of Plant Interactions, 9(1): 354-363. (Journal)
Ober, E. S. and Sharp, R. E. 2003. Electrophysiological responses of maize roots to low water potentials: relationship to growth and ABA accumulation. Journal of Experimental Botany, 54 (383): 813-824. (Journal)
Oracz, K., Bailly, C., Gniazdowska, A., Côme, D., Corbineau, D. and Bogatek, R. 2007. Induction of oxidative stress by sunflower phytotoxins in germinating mustard seeds. Journal of Chemical Ecology, 33: 251-264. (Journal)
Penfield, S., Josse, E. M., Kannangara, R., Gilday, A. D., Halliday, K. J. and Graham, I. A. 2005. Cold and light control seed germination through the bHLH transcription factor SPATULA. Current Biology, 156(1): 1998–2006. (Journal)
Pessarakli, M. 2011. Handbook of Plant and Crop Physiology. Second Edition Revised and Expanded. Marcel Dekker, Inc. All Rights Reserved.997 P. (Book)
Pucciariello, C., Banti, V. and Perata, P. 2012. ROS signaling as common element in low oxygen and heat stresses. Plant Physiology and Biochemistry, 59: 3 -10. (Journal)
Rylott, E. L., Hooks, M. A. and Graham, I. A. 2001. Co-ordinate regulation of genes involved in storage lipid mobilization in Arabidopsis thaliana. Biochemical Society Transactions, 29: 283–287. (Journal)
Sedghi, M. 2016. Plant biochemistry. Publications of the University of Mohaghegh Ardabili, Iran. P: 420. (Book)
 
Srinivasara, C. H., Wani, S. P., Sahrawat, K. L., Rego, T. J. and Pardhasaradhi, G. 2008. Zinc, boron and sulphur deficiencies are holding back the potential of rain fed crops in semi-arid India: Experiments from participatory watershed management. International Journal of Plant Production, 2(1): 89-99. (Journal)
Turk‚ M. A‚ Rahmsn‚ A.‚ Tawaha‚ M. and Lee‚ K. D. 2004. Seed germination and seedling growth of three lentil cultivars under moisture stress. Asian Journal of Plant Sciences, 3: 394-397. (Journal)
Turkan, I. 2011. Plant responses to drought and salinity stress: Development in a post-genomic era. Academic press. New York. (Book)
Waraich, E. A., Amad, R., Ashraf, M., Saifullah, Y. and Ahmad, M. 2011. Improving agricultural water use efficiency by nutrient management. Acta Agriculturae Scandinavica, 61(4): 291-304. (Journal)
Woodger, F., Jacobsen, J. V. and Gubler, F. 2004. Gibberellin action in germinating cereal grains. In: Plant Hormones: Biosynthesis, Signal Transduction, and Action. (Ed. P.J. Davies), pp. 221–240. Kluwer Academic Publishers, Dordrecht. (Book)
Yu, J. Q., Fye, S., Zhang, M. F. and Hu, W. H. 2003. Effects of root exudates and aqueous root extract of cucumber and allelochemicals on photosynthesis and antioxidant enzymes in cucumber. Biological Systems and Ecology, 31: 129-139. (Journal)