The effect of chilling treatment on physiological traits of germination of three soybean cultivars

Document Type : Research Paper

Authors

1 Karaj

2 Ardabil

Abstract

Due to sensitivity of soybean seedlings to cold, in order to study the effect of chilling duration and cultivar on physiological characteristics of soybean seed germination, a factorial trial in random block design with five level of pre-chilling (control, 10, 20, 30, 40 days in 4°C) and three cultivars (JK, Linford and Clean) with three replication was conducted in the seed science and technology laboratory of Mohaghegh Ardebili University in 2014. Traits like the percentage of  germination, germination rate, Sodium (Na) content, Calcium (Ca) content, Potassium (K) content,  Electrical conductivity (EC), soluble sugar content, Catalase and Peroxidase enzyme activity were measured. The interaction of cultivar and pre-chilling was significant on all biochemical traits of seed germination except Peroxidase and Catalase enzyme activity and EC. Highest germination percentage and rate happened in 40 days pre-chilling treatment. The EC of seeds was increased by 27 percent with increase in the duration of chilling from control (no chilling) to 40 days chilling. Cultivar and chilling interaction on inorganic elements Na, K and Ca were significant, the percentage of Na decreased and percentage of K and Ca increased with increase in duration of chilling. 40 days of chilling caused 8.5 percent increase in soluble sugar compare to control (no chilling). The activity of antioxidant enzymes Catalase and Peroxidase, rose with the increase in duration of chilling. The highest activity of Catalase enzyme was gained from the cold resistant cultivar (JK) with 8.507 µM/min*g (fresh weight) and the highest Peroxidase enzyme activity yielded from 40 days chilling treatment with amount of 37.243 µM/min* g (fresh weight). According to the physiological result the 40 days chilling treatment, due to high germination percentage and rate, high antioxidant enzyme activity and soluble sugar, is recommended for sowing and better seedling establishment.

Keywords

References

Abe, H. 1984. Catalase in vitro. Method of Enzymology, 105:121-126. (Journal)
Ahmadpour Dehkordi, S. and Balouchi, H.R. 2012. Effect of seed priming on antioxidant enzymes and lipids peroxidation of cell membrane in Black cumin (Nigellasativa) seedling under salinity and drought stress. EJCP, 4: 63-85. (Journal)
Amooaghaie, R. 2009. The effect of soaking, temperature and duration of pre-chilling on seed dormancy breaking of Ferula ovina. Iranian Journal biological Science,18(4): 350-358.(In Persian)(Journal)
Ashraf, M. and Khanum, A. 1997. Relationship between ion accumulation and growth in two spring wheat lines differing in salt tolerance at different growth stages. Argon and Crop Sci, 178: 39-51. (Journal)
Atici, O., Jim, R and Nalbantoglu, B. 2003. Antifreeze proteins in higher plants. Phytochemist, 64:1187-1196. (Journal)
Baek, S.H. and Kwon, S. 2000. Activities and isozyme profiles of antioxidant enzymes in intercellular compartment of overwintering barley leaves. Journal of Biochemistry and Molecular Biology, 5: 385-90. (Journal)
Bailly, C. 2004. Active oxygen species and antioxidants in seed biology. Seed Science Research, 14:93–107. (Journal)
 
 
Beltagi, M.S. 2008. Exogenous ascorbic acid (vitamin C) induced anabolic changes for salt tolerance in chick pea (Cicer arietinum L.) plants. African Journal of Plant Science, 10: 118-123. (Journal)
Bhattacharjee, S. 2005. Reactive oxygen species and oxidativeburst: Rol in stress, senescence and signal transduction in plants. Journal of current Science, 89: 1113-1121. (Journal)
Brogan, J.C. 2006. Flame photometric determination of calcium in plants. Journal Science Food Agricultures, 11: 446 - 449. (Journal)
Cardona, C.A., Duncan, R.R and Lindstrom, O. 1997. Low temperature tolerance assessment in Paspalum. Crop science, 37:1283-1291. (Journal)
Chhipal, B.R and Lal, P. 1995. Na+/K+ ratios the basis of salt tolerance in wheat. Aust Journal Agric Res, 46:533-539. (Journal)
El-Kassaby, Y and Edwards, A. 1981. Genetic control of germination and the effect of accelerated agening in mountain hemlock seeds and its relevance to gene conservation. Forest ecology and management, 112: 203-211. (Journal)
Ellis, R.H. and Roberts, E.H. 1981. The quantification of aging and survival in orthodox seeds. Seed Sci. Technol, 9: 373-409. (Journal)
Esfandiari, E., Shekari, M. and Esfandiari, f. 2007. The effect of salt stress on antioxidant enzymes activity and lipid peroxidation on the wheat seedling. Journal of Notulae Botanicae Horti Agribotanici Cluj-Napoca,35:48-56. (Journal)
Fessel, S.A., Vieira, R.D., Cruz, M.C.P., Paula, R.C. and Panobianco, M. 2006. Electrical conductivity testing of corn seeds as influenced by temperature and period of storage. Pesq Agropec Bras, 41: 1551-1559. (Journal)
Ghorbani, A., Zarinkamar, F. and Fallah, A. 2011. Effect of Cold Stress on the Anatomy and Morphology of the Tolerant and Sensitive Cultivars of Rice during Germination. Journal of Cell and Tissue, 3: 235-244. (Journal)
Greipson, S. and Davy, A. 1996. Aspects of seed germination in the dune-building grass Leymus arenarius. Icelandic Agriculture Science, 10: 209-217. (Journal)
Gunn, S. and Farrar, J.F. 2002. Effects of a 4 °C increase in temperature on partitioning of leaf area and dry mass, root respiration and carbohydrates. Journal Functional Ecology, 13 s1: 12-20. (Journal)
Guo, Z., Ou, W., L.u, S. and Zhong, Q. 2006. Differential responses of ant oxidative system to chilling and drought in four rice cultivars differing in sensitivity. Journal of plan physio and biochip, 44:828-836. (Journal)
Horváth, E., Szalai, G. and Janda, T. 2007. Induction of abiotic stress tolerance by salicylic acid signaling. Plant Grow Regul, 26:290-300. (Book).
Hoseinpour Ghazvini, A. 2011. Investigation germination problem and seed viability four savory ecotype in laboratory and field situation. Takistan Islamic Azad University (Thesis)
Jahanbakhsh-Godehkahriz, S., Karimzadeh, G.H., Rastgar, F., Mahfoozi. S. and Hosseini-Salekdeh, G.H. 2009. Influence of Verbalization on some physiological Characteristics and Cold Tolerance in two Susceptible and Tolerant Cultivars of Bread Wheat. EJCP. 3: 85-106. (Journal)
Janda, T., Szalai, G., Tari, I. and Paldi, E. 1999. Hydroponic treatment with salicylic acid decreases the effects of chilling injury in maize (Zea mays L.) plants. Planta, 208: 175- 180. (Journal)
Jubany-Marí, T., Munné-Bosch, S. and Alegre, L. 2010. Redox regulation of water stress responses in field-grown plants. Role of hydrogen peroxide and ascorbate. Plant Physiology and Biochemistry, 48: 351-358. (Journal)
Kaur, S., Gupta, A.K., Kaur, N., Sandhu, J.S. and Gupta, S.K. 2009. Anti-oxidative Enzymes and Sucrose Synthase Contribute to cold stress Tolerance in chichpea. Journal of Agrono and Crop Scien, 5: 393-397. (Journal)
Kerepesi, I., Bányai-Stefanovits, E. and Galiba, G. 2004. Cold acclimation and abscisic acid induced alterations in carbohydrate content in calli of wheat genotypes differing in frost tolerance. Plant Physiol, 161: 131-133. (Journal)
 
 
Khanna, C.R., Vasudev, S.M., Maheswari, M., Srivastava, A. and Bahukhandi, D. 1995. K+ osmo regulation and drought tolerance: an overview. Proc. Indian Nat Sci Acad. 61: 51–56. (Journal)
Kozlowski, T.T. and Pallardy, S.G. 2002. Acclimation and adaptive responses of woody plants to environmental stresses. The Botanical Review, 68: 270-334. (Journal)
Kucera, B., Cohn, M.A. and Leubner-Metzger, G. 2005. Plant hormone interactions during seed dormancy release and germination. Seed Scien Resear, 15: 281-307. (Journal)
Kuk, Y.I., Shin, J.S., Burgos, N.R., Hwang, T.E., Han, O., Cho, H.B., Jung, S. and Guh, J.O. 2003. Anti-oxidative enzymes offer protection from chilling damage in rice plants. Crop Scien, 163: 2109-2117. (Journal)
Kumar, S., Malik, J., Thakur, P., Kaistha, S., Sharma, K.D., Upadhyaya, H.D., Berger, J.D. and Nayyar, H. 2010. Growth and metabolic resposes of contrasting chichpea (cicer arientinum L.) Genotypes to chilling stress at reproductive phase, Acta phisiol, 3:779-787. (Journal)
Levent-Tuna, A., Kaya, C., Ashraf, M., Altunlu, H., Yokas, I. and Yagmur, B. 2007. The effects of calcium sulphate on growth, membrane stability and nutrient uptake of tomato plants grown under salt stress. Journal Exp Bot, 59: 173-178. (Journal)
Macadam, J.W., Nelson, C.J. and Sharp, R.E. 1992. Peroxidase Activity in the leaf Elongation Zone of Tall Fescue. Plant physio, 99:872-878. (Journal)
Mahajan, S. and Tuteja, N. 2005. Cold, salinity and drought stresses: An overview. Arch. Biochem. Bioph, 444: 139-158. (Journal)
Maser, P., Gierth, M. and Schroeder, I.J. 2002. Molecular mechanisms of potassium and sodium uptake in plant. Plant Soil, 247: 43-54. (Journal)
Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci, 7: 405-410. (Journal)
Mokhtary, I., Abrishamchi, P. and A., Ganjali. 2004. Ameliorative effects of CaCl2 and CaSO4 on growth, content of soluble proteins, soluble sugars, proline and some mineral nutrients (Na+,K+) in leaves of Lycopersicon esculent vary Mobile under salt stress. Journal of biochemistry Iran. 1:62-73.(In Persian)(Journal)
Munns, R. and James, R.A. 2003. Screening methods for salt tolerance: a case study with tetraploid wheat. Plant and Soil, 253: 201–218. (Journal)
Nadjafi, M., Banayan, L., Tabrizi, I. and Rastgoo, M. 2006. Seed germination and seed dormancy breaking techniques for Ferula gummosa and Teucrium polium. 30: 508-512. (Journal)
Nasiri, M. 1994. Investigation of effective on germination, development seeds. Organization research and teaching agriculture.63 Pp. (Book)
Nezami, A. and sharef, A. 2007. Leakage electrolyte as title index during chilling damage on canola, Journal agronomy research Iran, 5: 3, 167-175. (Journal)
Pasandideh, H. 2010. Assessment seed vigor and planet commercial soybean cultivar with different method. M.Sc. Thesis, seed science and technology Mohaghe Ardabili University. 119 Pp. (?)
Paul, M. and Hasegava, A. 1996. Plant cellular and molecular responses to high salinity. Annual Review of  Plant Physiology, 51: 463-499. (Journal)
Pessarakli, M. 1999. Hand book of plant and crop stress. Marcel Dekker. (Book).
Qu, X., Baskin, J.M., Wang, L. and Huang, Z. 2008. Effects of cold stratification, temperature, light and salinity on seed germination and radicle growth of the desert halophyte shrub, Kalidium caspicum (chenopodiaceae). Plant Growth Regul, 54: 241-248. (Journal)
Rawat, B.S., Khanduri, V.P. and Sharma, C.H.M. 2008. Beneficial effects of cold-moist stratification on seed germination behaviors of Abies pindrow and Picea smithiana. Journal of Forestry Research, 19 (2): 125-130. (Journal)
Roe, J.H. 1995. The determination of sugar in blood and spinal fluid with anthrone reagent. J. Biol. Chem, 212: 335-343. (Journal)
 
Rouhi, H.R., Aboutalebian, M.A., Moosavi, S.A., Karimi, F.A., Karimi, F., Saman, M. and Samadi, M. 2012. Change in several antioxidant enzymes activity of Berseem clover (Trifolium alexandrinum L.) by priming. International Journal of Agri Science, 3: 237- 243. (Journal)
Sajadi, S. 2011. Investigation of Potential germination and seed vigor some of chamomile population with use of removal treatment seed dormancy. Mohaghe Ardabili University. 119Pp.(Thesis)
Samach, A. and Wigge, P.A. 2005. Ambient temperature perception in plants. Current Opinion in Plant Biology, 8: 483–486. (Journal)
Sanders, D., Pelloux, J., Brownlee, C. and Harper, J.F. 2002. Calcium at the cross roads of signaling. Plant Cell, 14: 401–417. (Journal)
Siosemardeh, A., Mohammadi, K.H., Roohi, E., Aghaalikhani, M. and Mokhtasi Bidgoli, A. 2009. Physiological responses of different wheat genotypes to cold stress. EJCP, 4: 93-112. (Journal)
Takac, T. 2004. The relationship of antioxidant enzymes and some physiological parameters in maize during chilling. Journal of plan soil and environ, 50:27-32. (Journal)
Tasgin, E., Atici, O., Nalbantoglu, B. and Petrova, L. 2006. Effects of salicylic acid and cold treatment on protein levels and on the activities on antioxidant enzymes in the apoplast of winter wheat leaves. Journal of phytochemis, 67: 710-715. (Journal)
Vanae, S., siosemardeh, A. and Haydare, G.H. 2011. Effect cold stress during germination and seedling on enzyme activity and some morphophysiological in pea. Journal agronomy research Iran, 9(3): 514-524. (Journal)
Wang, W.B., Kim, Y.H., Lee, H.S., Yong Kim, K., Deng, X. and Kwak, S. 2009. Analysis of antioxidant enzyme activity during germination of alfalfa under salt and drought stresses. Journal of plant physio and Biochimis, 47: 570-577. (Journal)
Wiersma, J.J. 2008. Winter wheat in Minnesota. University of Minnesota Extension service. (Book)
Wu, R. and Gang, A. 2003. Engineering rice plants with trehalose producing genes improves tolerance to drought, salt and low temperature. ISB News Reports, http://www.isb.vt.edu/news/2003/news03.mar.html.
Yong, Z., Hao-Ru, T. and Ya, L. 2008. Variation in antioxidant enzyme activites of two strawbreey cultivars with short-term low temperature stress. Journal of Agricultur Scien, 4: 456-462. (Journal)
Zeinali Yadegari, L., Heidari, R. and Carapetian, J. 2009. Changes in Membrane permeability and Antioxidative Enzymes Activity in Response to Low Temperature in Soybean Seedlings, journal biology Iran, 22(2); 229-236. (Journal)
Iranian Journal of Seed Sciences and Research
Volume 4, Issue 4
February 2018
Pages 115-130

Files

Share

How to cite

Statistics