The effect of seed inoculation with Azotobacter and Azospirillum and application of urban sewage sludge on some of the qualitative and quantitative characteristics of the ‌( Brassica napus L. )

Document Type : Research Paper

Authors

1 Ph.D student of Agronomy, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

2 Faculty member

Abstract

In order to investigate the effect of Azotobacter, Azospirillum and application of urban sewage sludge on some of the qualitative and quantitative characteristics of the Rapeseed (Brassica napus L.), an experiment was carried out in crop years 2014-2015 and 2015-2016 on the lands of  Water and Wastewater Company of southern Tehran (Rey city). This experiment was conducted in a factorial way based on random complete block design with three replications. Experimental factors included, inoculation of rapeseed (Without inoculation, inoculation with Azotobacter choroccocum, inoculation with Azospirillum. Brasilense) and use of sewage sludge recovered: without nitrogen fertilizer and without using sewage sludge, using nitrogen fertilizer, 10, 20 and 30 tons of sewage sludge per hectare.The results indicated that the effect of inoculation and sewage sludge on stem dry weight, biological yield, number of pods per plant, number of seeds in pod, 1000 seed weight, seed yield and acid oleic and linoleic percent was significant at (1% level). The results indicated that the highest oil seed canola was obtained at 10 ton sewage sludge and non-inoculation treatment (38.75 %) and the highest oil yield was obtained at 30 ton sewage sludge and inoculation with Azotobacter treatment with amount of 932.77  kilogram per hectare.The results indicated that the urban sewage sludge application was effective on qualitative and quantitative yield of Brassica napus and inoculation with Azotobacter had better effect than Azospirillum on the traits being investigated such as oil and protein yield.

Keywords

References

Ahmadi, M. and M.J. Bahrani. 2009. Yield and yield components of rapeseed as influenced by water stress at different growth stages and nitrogen levels. Am-Euras. J. Agric. Environ. Sci. 5 (6): 755-761.
Atlas, RM.1995. Petroleum biodegradation and oil spill bioremediation. Marine Poll Bull 31, 178-182.
Banga, R.S., R.S. Bisht and A. Yadav. 2007. Variation in growth rate and seed yield of Brassica juncea genotypes as affected by nitrogen levels. Proceedings of the 12th International Rapeseed Congress, China, 3: 246-247.
Basalma D. 2008. The correlation and path analysis of yield and yield components of different winter rapeseed (Brassica napus L.) cultivars. Research Journal of Agricultural and Biological Science. 4 : 120-125.
Berry, M. P., and Spink, J.H. 2006. A physiological analysis of oilseed rape yield , past and future (review). Journal of Agricultural Science,Cambridge 199: 381-392.
Cakmakci, R., Erat, M., Erdoman, U.G., and M.F. Donmez. 2007b. The influence of PGPR on growth parameters, antioxidant and pentose phosphate oxidative cycle enzymes in wheat and spinach plants. J. Plant Nutr. Soil Sci. 170: 288-295.
Chen Y.M., Wang M.K., Zhuang S.Y., and Chiang P.N. 2006. Chemical and physical properties of rhizosphere and bulk soils of three tea plants cultivated in Ultisols. Geoderma 136: 378–387.
El-Ghadban EAE, Shalan MN and Abdel-Latif TAT. 2006. Influence of biofertilizers on growth, volatile oil yield and constituents of fennel (Foeniculum vulgare Mill.). Egyptian Journal of Agricultural Research. 84(3):977- 992.
EL-Zeiny, O. A. H. 2007. Effect of biofertilizers and root exudates of two weed as a source of natural growth regulators on growth and productivity of bean plants (Phaseolus vulgaris L.). Journal of Agriculture and Biological Science 3(5): 440-446
Esmaeil, Y. and A. M. Patwardhan. 2006. Physiological analysis of the growth and development of canola (Brassica napus L.) under different chemical fertilizer application. Asian Journal of Plant Science 5: 745-752.
Gaur, A. C. 2001. Effects of Azotobacterization on the yield of canola (Brassica napus L.): Laboratory experiment. Indian Society of Soil Science 40: 19-22
Gryndler, M., J. Larsen, H. Hrselova, V. Rezacova, H. Gryndlerova and J. Kubat. 2006. Organic and mineral fertilization, respectively, increase and decrease the development of external mycelium of arbuscular mycorrhizal fungi in a long-term field experiment. Mycorrhiza, 16: 159–166.
Guerzoni, M.E., R. Lanciotti and R.S. Cocconcelli. 2001. Alteration in cellular fatty acid composition as a response to salt, acid, oxidative and thermal stresses. Microbiology. 147: 2255-2264.
Hanh, T.H., Kim, J.Y., Bang, S., & Kim, K.W. 2010. Sources and fate of As in the environment. Geosystem Engineering,13, 35–42.
Heshmatpure, N., and Yousefi Rad, M. 2012. The effect of PGPR (Plant-Growth-Promoting Rhizobacteria) on phytoremediation of cadmiums by canola (Brassica napus L.) cultivars of Hyola 401. Annals of Biological Research 3 (12):5624-563.
Karamanos, R.E., Goh, T.B., and Poisson, D.P. 2006. Nitrogen, Phosphorus and Sulfur fertility of hybrid Canola. Journal of Plant Nutrition, 28: 1145-1161.
Karami, M., Rezainajad, Y., Afyuni, M., and Shariatmadari, H. 2007. Cumulative and residual effects of sewage sludge on lead and cadmium concentration in soil and wheat. J. Sci. Technol. Agric. Natur. Resour. 11: 1. 79-95. (In Persian) .
Kaymak, H. A., I. Guvenc, F. Yarali and M. F. Denmez. 2009. The effects of bio-priming with PGPR on germination of radish (Raphanus sativus L.) seeds under saline conditions. Turk. J. Agric. 33: 173-179.
Kazemeini, S.A., Hamzehzarghani, H., and Edalat, M. 2010. The impact of nitrogen and organic matter on winter canola seed yield and yield components. Australian Journal of Crop Science, 4(5): 335-342.
Khajehpour, M. R. 2005. Industrial Crops. Jahade-e-Daneshghahi Isfahan Press. (In Persian).
Khosravi, H. 2007. Investigating the frequency and distribution of Azotobacter in agricultural soils of Tehran and studying some of its physiological characteristics. MSc Thesis.Tehran university.111 page.
Kutcher, H.R., S.S. Malhi and K.S. Gill. 2005. Topography and management of nitrogen and fungicide affects diseases and productivity of canola. Agron. J. 97(2): 533-541.
Lasat MM, 2002. Phytoextraction of toxic metals; a review of biological mechanisms. J Environ Qual 31: 109-120.
Martin, X. M., C. S. Sumathi and V. R. Kannan. 2011. Influence of agrochemicals and Azotobacter sp. Application on soil fertility in relation to maize growth under nursery conditions. European Asian Journal of Biosciences 5: 19-28.
McGrath SP, Zhao FJ and Lombi E, 2001. Plant and process in phytoremediation of metal contaminated soils. Plant and Soil 232: 207- 214.
Mohammadi, T., M. H. Azizi and A. Taslimi. 2007. Relation of fatty acids composition with stability of sunflower and canola oil blends. J. Food Sci. Technol. 4: 67–76.
Moradi R., Rezvani Moghaddam C., Nasiri Mahalati M. Lakzaian A. 2009. Effect of biological and organic fertilizers on yield, yield components and essential oil of fennel (Foeniculum vulgare). Journal of Agricultural Research, 7(2): 625-635. (In Persian).
Morera, M. T., J. Echeveria and J. Garrido. 2002. Bioavailability of heavy metals in soil amended with sewage sludge. Canadian Journal of Soil Science, 82: 433-438.
Ngezimana, W., and Agenbag, G.A. 2013. Effects of nitrogen (N) and sulphur (S) on canola (Brassica napus L.) vegetative and reproductive growth under controlled conditions. African Journal of Agricultural Research, 8(39): 4887-4894.
Oliveira A and Pampulha ME, 2006. Effects of long-term heavy metal concentration on soil microbial characteristics. J Biosci Bioeng 102: 157-161.
Ozyazici,  M. A. 2013. Effects of sewage sludge on the yield of plants in the rotation system of wheat-white head cabbage-tomato. Eurasian Journal of Soil Science, 2: 35–44.
Pakdel M., Malaki A., Normohamadi G.h., Fazel S.h. 2011. PGPR effect of Azotobacter and Pseudomonas aeruginosa on yield and yield components of wheat in normal and drought conditions. Journal of Crop Science, 3(11): 107- 121. (In Persian).
Rabiee, M., M. Kavoosi and P. Tousi Kehal. 2012. Effect of nitrogen fertilizer levels and their application time on yield and some agronomic traits of rapeseed (cv. Hyola 401) in winter cultivation in Guilan. Journal of Science and Technology of Agriculture and Natural Resources, Water and Soil 15(58): 199-212. (In Farsi).
Raymer PL ( 2002) “ canola: on emerging oilseed crop” in : Trends in new crops and new uses . Ganick, J. And Whipkey , A. ASHS Press. Alexandria, VA,122-126.
Sadhasivam, S., S. Savitha, and K. Swaminathan. 2007. Exploitation of Trichoderma harzianum mycelial waste for the removal of rhodamine 6G from aqueous solution.J.Environ. Manage. 5:155–161.
Shaharoona, B., Arshad, M., Zahir, Z.A and Khalid, A., 2006. Performance of pseudomonas spp. containing acc-deaminase for improving growth and yield of maize (Zea mays L.) in the presence of nitrogenous fertilizer. Soil Biol.Biochem. 38: 2971-2975.
Sharma, A. K. 2003. Biofertilizers for sustainable agriculture. Agro bios, India.
Sharma and Rehman M. B., 2009. Laboratory scale approach, Indian Journal of Microbiology, 48: 142-146.
Sharma, S. 2012. Bioremediation: Features, Strategies and applications. Asian Journal of Pharmacy and Life Science. 2 (2): 46-59.
Shen J., Rengel Z., Tang C., and Zhang F. 2003. Role of phosphorus nutrition in development of cluster roots and release of carboxylate in soil grown Lupinus albus. Plan and Soil, 248: 199-206.
Sirjani, A., Farahbakhsh, H., Ravari, Z., Pasandipour, N., and Karami, A. 2011. Investigation the effect of biofertilizer consumption, zinc sulfate and nitrogen fertilizer on quantitative and qualitative yield of wheat. Iranian Journal of Soil Research (Soil and Water Sciences), 25(2), 125-135, (In Persian).
Teparut, C. and Sthiannopkao, S. 2011. Mae Moh lignite mine and environmental management. Geosystem Engineering,14, 85–94.
Turan M Gulluce M Karadayi M Baris O and Sahin. F. 2011. Role of soil enzymes produced by PGPR strains in wheat growth and nutrient uptake parameters in the filed conditions. Abstracts/Current Opinion in Biotechnology. 22: 15–152.
Vaseghi, S., Afyouni, M., Shariat Madari, H., and Mobli, M. 2005. “Effect of sewage sludge on same macronutrients concentration and soil chemical properties.” J. of Water and Wastewater, 53, 18-25.(In Persian) .
Violent, H.G.M., and Portugal, V.O. 2007. Alternation of tomato fruit quality by root inoculation with plant growth-promoting rhizobactria (PGPR): Bacillus subtilis BEB-13bs. Sci Hort. 113: 103-106.
Xu, N., X. Zhang, X.Fan, L. Han and C. Zeng. 2001. Effect of nitrogen source and concentration on growth rate and fatty acid composition of Ellipsioidion SP. (Eustigmatophyta). Journal of Applied Physiology 13: 463-469.
Yasari , E., M. R. Azadgoleh, S. Mozafari and M. Alashti. 2009. Enhancement of growth and nutrient uptake of rapeseed (Brassica napus L.) by applying mineral nutrients and biofertilizers. Pakistan Journal of Biological Science 15: 12(2): 127-33.
Zahir, A.Z., M., Arshad, and W.F. Frankenberger. 2004. Plant growth promoting rhizobacteria. Application and perspectives in agriculture. Adva in Agron. 81: 97-168.
Iranian Journal of Seed Sciences and Research
Volume 4, Issue 4
February 2018
Pages 73-86

Files

Share

How to cite

Statistics