Effect of hormone priming and hydro priming on Stevia (Stevia rebaudiana Bertoni) seed germination under salt stress

Document Type : Research Paper

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Abstract

     In order to evaluate effect of time period of hormone and hydro priming on Stevia seed germination indices under salt stress a factorial experiment based on completely randomized design (CRD) with three replications was conducted in Seed Science and Technology Laboratory of Shahed University of Tehran in 2015. The studied factors consisted of seed pre-treating by gibberellic acid and distilled water for 0, 16, 24 and 48 hours and salt stress (2.5, 5, 7.5 and 10 dS.m-1). Germination percentage and speed, mean time of germination, mean of daily germination, daily germination speed and germination value was measured. Results showed that salinity× hormone priming interaction significantly influenced germination percentage and germination speed, mean daily germination and germination value. The highest germination percentage and germination speed was seen in 5 dS.m-1 and 48 hours hormone priming interaction and 2.5 dS.m-1 and 48 hours hormone priming interaction respectively. The highest daily germination speeds was seen in 0 dS.m-1 and 16 hours hydro priming interaction and 0 dS.m-1 and 16 and 24 hours hormone priming interaction respectively. Hormone priming of stevia seed by gibberellic acid for 24 and 48 hours could improve stevia seed germination parameters. Some of germination parameters (rate and percentage of germination) were reduced by salinity stress.

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References

Afzal, I. 2005. Seed enhancements to induced salt tolerance in wheat (Triticum aestivum L.). Ph.D. Thesis, Agricultural University of Faisalabad, Pakistan.(Thesis)
Anonymous, 2011. Utilization of saline-alkaline land. Department of Education Vocational.pp:40. (In Persian)(Handbook)
Artola, A., Carrillo-Castaneda, G. and Santos, G.D. 2003. Hydro-priming: strategy to increase Lotus corniculatus L. seed vigor. Seed Science and Technology, 31: 455-463. (Journal)
Ashraf, M., Athar, H.R., Harris, P.J.C. and Kwon, T.R. 2008. Some prospective strategies for improving crop salt tolerance. Advanced in Agronomy, 97: 45-92. (Journal)
Basra, S.M., Farooge, M., Tabassum, R. and Ahmed, N. 2005. Physiological and biochemical aspects of pre-sowing seed treatments in fine rice (Oryza sativa L.). Seed Science and Technology, 33: 877-879. (Journal)
Brancalion, P.H.S., Novembre, A.D.L.C., Rodrigues, R.R. and Tay, D. 2008. Priming of Mimosa bimucronata seeds: a tropical tree species from Brazil, Acta Horticulturae, 82: 163-168. (Journal)
Chalapathi, M.V., Thimmegowda, S., Rama Krishna Prama, V.R. and Prasad, T.G. 1997. Natural non-calorie sweetener Stevia (Stevia rebaudiana Bertoni): a future crop of India. Crop Research, 14: 347–350. (Journal)
Copland, L.O. and McDonald, M.B. 1995. Principles of Seed Science and Technoloy. Thired Edition. Springer US Publisher. (Book)
Eisavand, H.R., Tavakol Afshari, R., Sharifzade, F., Madah Arefi, H. and Hesamzade Hejazi, M. 2008. Improving physiological quality of aged Wheat Grass seed by using hormonal priming under water stress and non-stress conditions. Iranian Journal Crop Science, 39: 53-65. (In Persian)(Journal)
El-Araby, M.M. and Hejazi, A.Z. 2004. Responses of tomato seeds to hydro- and osomo- priming and possible relations of some antioxidant enzyme and endogenous polyamine fractions. Egyptian Journal of Biology, 6: 81-93. (Journal)
Ellis, R.H. and Roberts, E.H. 1981. The quantification of ageing and survival in orthodox seeds. Seed Science and Technology, 9: 377-409. (Journal)
Eslami, V., Behdani, M.A. and Ali, S. 2009. Effect of salinity on germination and early seedling growth of canola cultivars. Environmental Stress in Agriculture Science, 1: 39-46. (In Persian)(Journal)
Farooq, M., Basra, S.M.A., Warraich, E.A. and Khaliq, A. 2006. Optimization of hydro-priming techniques for rice seed invigoration. Seed Science and Technology, 34: 529-534. (Journal)
Fathi Amirkhiz, K., Omidi, H., Heshmati, S. and Jafarzadeh, L. 2012. The effect of catalyst on the vigor and germination properties of the herb Nigella (Nigella sativa L.) under salt stress. Iranian Journal of Field Crops Research, 10: 299-310. (Journal)
Foti, R., Abureni, K., Tigere, A., Gotosa, J. and Gere, J. 2008. The efficacy of different seed priming osmotica on the establishment of maize (Zea mays L.) caryopses, Journal of Arid Enviroments, 72: 1127-1130. (Journal)
Ghasemi gholozani, K. and Dalil, B. 2011. Germination and seed vigor tests. Publications Jahad Daneshgahi Mashhad. (In Persian)(Book)
Goettemoeller, J. and Ching, A. 1999. Seed germination in Stevia rebaudiana. In:  Janick, J (eds)  Perspectives on new crops and new uses. ASHS Press, Alexandria, VA. Pp: 510–511. (Book)
Hassanpoure, H. and Darvishi, H. 2011. Effect of saline water on quantitative and qualitative traits of Dill (Aniethum graveolens L.) seeds. Journal of Agronomy and Plant Breeding, 6: 13-20. (In Persian)(Journal)
Hoogenboom, G. and Peterson, C.M. 1987. Shoot growth rate of soybean as affected by drought stress. Agronomy Journal, 79:5 98-607. (Journal)
Hossein, M.A., Shamim Kabri, A.M., Jahan, T.A. and Hassan, M.N. 2008. Micropropagagation of Stevia Rebaudiana. International Journal of Sustainable Crop Production, 3: 1-90. (Journal)
Kafi, M., Eishi Rezaii, A., Hagighikhah, M. and Gorbani, S. 2010. Effect of salinity and seed priming on germination and seedling characteristics of two medicinal citrus species. Journal of Agriculture Ecology, 2: 245-255. (In Persian)(Journal)
Khan, M.A. and Ungar, I.A. 2001. Seed germination of Triglochin maritime as influenced by salinity and dormancy relieving compounds. Biologia Plantarum, 44: 301-303. (Journal)
Kim, S.G. and Park, C.M. 2008. Gibberellic acid-mediated salt signaling in seed germination. Plant Signaling Behavior, 3: 877-879. (Journal)
Koornneef, M., Bentsink, L. and Hilhorst, H. 2002. Seed dormancy and germination. Current Opinion in Plant Biology, 5: 33-36. (Journal)
Lima, W.A.A., Dias D.C.F.S. and Cecon, P.R. 2003. Controlled hydration for priming in coffee (Coffea Arabica L.) seeds. Seed Science and Technology, 31: 29-37. (Journal)
Liopa-Tsakalidi, A., Kaspiris, G., Salahas, G. and Barouchas, P. 2012. Effect of salicylic acid (SA) and gibberellic acid (GA1) pre-soaking on seed germination of Stevia (Stevia rebaudiana) under salt stress. Journal of Medicinal Plants Research, 6: 416-423. (Journal)
Maiti, R.K. and Purohit, S.S. 2008. Stevia: A miracle plant for human health Agro bios (India) Jodhpur India. (Book) 
Miyagawa, H., Fujikowa, N., Kohda, H., Yamasaki, K., Taniguchi, K. and Tanaka, R. 1986. Studies on the tissue culture of Stevia rebaudiana and its components: (II). Induction of shoot primordia. Planta Med, 4: 321–324. (Journal)
Oddone, B. 1997. ‘‘How to Grow Stevia. Technical Manual.’’ Guarani Botanicals, Pawtucket, CT. (Book)
Pagter, M., Bragato, C., Malagoli M. and Brix, H. 2009. Osmotic and ionic effects of NaCl and Na2SO4 salinity on Phragmites australis. Aquatic Botany, 90: 43-51. (Journal)
Raeeszadeh, M. and Gharineh, M.H. 2014. Effects of gibberellic acid, nitric acid and moist chilling on seed germination Stevia. First International Congress and the Thirteenth National Congress of the Plant Breeding and Seed Science and Technology Conference. Tehran 4 to 6 September. (In Persian)(Conference)
Raina, R., Bhandari, S.K., Chand, R. and Sharma, Y. 2013. Strategies to improve poor seed germination in Stevia rebaudiana, a low calorie sweetener. Journal of Medicinal Plants Research, 7: 1793-1799. (Journal)
Raji, A.A., Mohammad, B.O. and Zarina, B.Z. 2015. Acclimatized apparatus enhanced seed germination in Stevia rebaudiana Bertoni. International Journal of Biology, 7: 28-34. (Journal)
Saedi, Z. 2013. Assess the impact of soil salinity and drought stresses on germination indices of Nepeta racemmosa L. Master Thesis of Agronomy. Alborz Payam Nor University. (In Persian)(Thesis)
Singh, S.D. and Rao, G.P. 2005. Stevia: the herbal sugar of 21st century. Sugar Technology, 7(1): 17-24. (Journal)
Sivirtepe, N., Sivirtepe, H.O. and Erifll, A. 2003. The effect of NaCl priming on salt tolerance in melon seedlings grown under conditions. Scientia Horticulturae, 97: 229- 237. (Journal)
Soltani, A., Gholipoor, M. and Zeinali, E., 2006. Seed reserve utilization and seedling growth of wheat as affected by drought and salinity. Environmental Experiment Botany, 55: 195-200. (Journal)
Stephanie, E.B., Svoboda, V.P., Paul, A.T. and Marc, W.V.I. 2005. Controlled drought affects morphology and anatomy of Salvia splendens. American Society for Horticultural Science, 130: 775-781. (Journal)
Sun, T.P. and Gubler, F. 2004. Molecular mechanism of gibberellin signaling in plants. Annual Review of Plant Biology, 55: 197-223. (Journal)
Takahashi, N., Phinney, B.O. and McMillan, J. 1991. Gibberellins, Springer-Verlag, New York. (Book)
Tobe, K., Li, M.X. and Omasa, K. 2004. Effects of five different salts on seed germination and seedling growth of Haloxylon ammodendron (Chenopodiaceae). Seed Science Research, 14: 345-353. (Journal)
Yosefi Tanha, M. 2014. The effect of priming to improve germination of winter annual green manure seeds under cold stress. Master Thesis of Seed Science and Technology. Shahrekord University. (In Persian)(Thesis)
Iranian Journal of Seed Sciences and Research
Volume 3, Issue 2
July 2016
Pages 97-108

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