Response of different Nigella Sativa L. genotypes to salinity stress at germination and seedling stages using multivariate analyses

Document Type : Research Paper

10.22124/jms.2026.9713

Abstract

In order to evaluate the response of germination components of Black Cumin (Nigella Sativa L.) genotypes to salinity stress, a factorial experiment was conducted based on a completely randomized design with four replications in the Plant Breeding Laboratory of Shahre-Kord University in 2023. The experimental factors included five levels of sodium chloride salinity (0, 50, 100, 150, and 200 mM) and 24 genotypes. The measured traits included germination percentage, germination rate, germination rate coefficient, mean germination time, root length, shoot length, Ratio of root to shoot length, root fresh weight, shoot fresh weight, root dry weight, shoot dry weight, ratio of dry weight of root to shoot, seedling Length vigor index and seedling weight vigor index. The results of Analysis of variance (ANOVA) showed that the effect of genotype, salinity stress and their interaction were highly significant difference for all studied traits (p<0.01). The results of principal components analysis (PCA) of the genotypes showed that the first four component accounted for 87% of the total variation. The cluster analysis using the wards method were able to classify lentil genotypes into two main groups. According to the results of cluster analysis and biplot, the genotypes G3, G2 and G72 were superior in terms of Length vigor index, seedling weight vigor index, shoot dry weight, germination percentage, germination rate and germination rate coefficient. Therefore, they were included in the group of the most tolerant genotypes. Genotypes G78, G11, G4, G59, G7 had average values in terms of the evaluated traits. Therefore, these genotypes are in the group of semi-tolerant genotypes to salt stress. Therefore, this group includes salt-sensitive genotypes such as G1, G9, G45, and G75.

Keywords

References

Abdul‐Baki, A. A., & Anderson, J. D. 1973. Vigor determination in soybean seed by multiple criteria 1. Crop science, 13(6), 630-633. (Journal)
Arzani, A. 2008. Improving Salinity Tolerance in Crop Plants: A Biotechnological View. In Vitro Cellular & Developmental Biology-Plant, 44, 373-383. (Journal)
Aziz, E.E., Hussein, M.S., Wahba, H.E., and Razin, A.M. 2013. Essential oil constituents of Dracocephalum moldavica L. grown under salt Stress and different sources of soil amendment. Middle-East Journal of Scientific Research, 16(5):706-713. (Journal)
Bayat, P., Ghobadi, M., Ghobadi, M.E. and Mohammadi, GH. 2016. Evaluation of the feasibility of a standard      seed germination test in vitro to predict emergence and establishment of chickpea (Cicer arietinum L.) seedlings in the field. Iranian Journal of Seed Science and Technology. 5(1): 27-38. (In Persian) (Journal)
Benakashani, F., Tavakoli, H., & Soltani, E. 2025. Living mulches as a strategy for weed control and improved Nigella sativa L. production in salt-affected soils. OCL, 32, 25. (Journal)
Bijeh Keshavarzi, M. H. 2012. Studying the effects of different levels of salinity which caused by NaCl on early growth and germination of Lactuca Sativa L. seedling. Journal of Stress Physiology & Biochemistry. 8(1): 203-208. (Journal)
Çavusoglu, K., and Kabar, K. 2010. Effects of hydrogen peroxide on the germination and early seedling growth of barley under NaCl and high temperature stresses. EurAsian Journal of BioSciences, 4. (Journal)
Co¸skun, Ö., Ça˘ glar, A.F., Çakır, B., and Gülseren, ˙I. 2021. Influence of Maillard reaction conditions and solvent extraction on the surface activity and foaming characteristics of black cumin protein concentrates. Journal of Food Science and Technology. 58(11): 4323–4332. (Journal)
Dadashi Chavan,D., Abbasi,A. and Ahmadi Lak, A. 2021. Evaluation of salinity tolerance in 43 lentil genotypes along with the people variety. Iranian Journal of Field Crop Science. 52(3): 67-87. (In Persian) (Journal)
Datta, J. K., Nag, S., Banerjee, A., and Mondai, N. K. 2009. Impact of salt stress on five varieties of wheat (Triticum aestivum L.) cultivars under laboratory condition. Journal of Applied Sciences and Environmental Management, 13(3): 93-97. (Journal)
Ellis, R. H. and E. H. Roberts. 1981. The quantification of ageing and survival in orthodox seeds. Seed Sci. Technol. 9(2): 373-409. (Journal)
Foroozanfar, M., Naghavi, M. R., Peyghambari, A., Jafari, A. A., and Kamalabadi, S. N. 2014. Evaluation of Medicago truncatula ecotypes for resistance to salinity. 22(1): 43-54. (In Persian) (Journal)
Hakimi, Y., Fatahi, R., Shokrpour, M., and Naghavi, M. R. 2022. Investigation of Germination Characteristics of Four Medicinal Plants Seed (Lavender, Hyssop, Black cumin and Scrophularia) Under Interaction Between Salinity Stress and Temperature Levels. Journal of Genetic Resources, 8(1), 35-45. doi: 10.22080/jgr.2021.21801.1262. (Journal)
Ikic, I., M. Maric evic, S. Tomasovic, J. Gunjaca, Z. S. Atovic, and H. S. Arcevic. 2012. The effect of germination temperature on seed dormancy in Croatian-grown winter wheats. Euphytica. 188: 25-34. (Journal)
Javadi, H., Thiqe El-Islami, M.J. and Mousavi, S.GH. 2014. Studying the effect of salinity on germination and early seedling growth of four medicinal plant species. Iranian Agricultural Research. 12(1): 53-64. (In Persian) (Journal)
Kabiri, R. and Naghizadeh, M. 2015. Investigating the effect of salicylic acid pretreatment on germination and early growth of black seed (Nigella sativa L.) seedlings under salt stress conditions. Iranian Journal of Seed Science and Technology. 4(1): 61-72. (In Persian) (Journal)
Kareem, N. J., Mohammed, B., Aziz, T., and Hussein, N. 2025. Response of black seed (Nigella sativa L.) varieties to seed priming with zinc on germination and seedling parameters. Journal of Medicinal and Industrial Plants, 3(2), 53-70. (Journal)
Maguire, J. D. 1962. Seed of germination – aid in selection and evaluation for seedling emergence and vigour. Crop science. 2: 176-177. (Journal)
Mohamadi, S.F., Babaeian Jelodar, N., Bagheri, N., Nematzadeh, Gh. and Hashemipetroudi, S.H. 2023. Studying the effect of salinity stress on some morphological and physiological traits in rice genotypes at the seedling stage under hydroponic conditions. Plant process and function. 59(13): 343-363. (In Persian) (Journal)
Mousavi, k., Khodarahmpour,Z. and Gilani, A. 2017. Grouping of rice genotypes on germination and seedling growth under salinity stress conditions. 4(1): 89-100. (In Persian) (Journal)
Papastylianou, P., Bakogianni, N. N., Travlos, I., and Roussis, I. 2018. Sensitivity of seed germination to salt stress in black cumin (Nigella sativa L.). Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 46(1), 202-205. (Journal)
Safarnejad, A., Sadr, S.V.A. and Hamidi, H. 2007. Effect of salinity stress on morphological characters of Nigella sativa. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 15(1): 75-84. (In Persian) (Journal)
Salamati, M.S. and Zeinali, H. 2013. Evaluation of genetic diversity of some Nigella sativa L. genotypes using Agro-morphological characteristics.  Iranian Journal of Medicinal and Aromatic Plants. 29(1): 201-214. (In Persian) (Journal)
Shakarami, B., Dianati-Tilaki, Gh., Tabari, M., and Behtari, B. 2011. The effect of priming treatments on salinity tolerance of Festuca arundinacea L. Schreb and Festuca ovina L. during seeds germination and early growth stages. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 18(2), 318-328. (Journal)
Soltani, A. and Khodarahmpour, Z. 2016. Evaluation of alfalfa genotypes for salt stress tolerance through multivariate statistical methods. Bi-Quarterly Journal of Plant Production. 6(1): 57-68. (In Persian) (Journal)
Tavousi, M., Anafcheh, Z. and Mahdavi Majd, J. 2020. The effect of different salinity levels on germination indices of 20 new quinoa genotypes. Environmental Stresses in Agricultural Sciences. 14(3): 837-847. (In Persian) (Journal)
Verma, S. K., G. C. Bjpai, S. K. Tewari, and J. Singh. 2005. Seedling index and yield as influenced by seed size in pigeon pea. Legume Res. 28(2): 143-145. (Journal)
Iranian Journal of Seed Sciences and Research
Volume 12, Issue 4
March 2026
Pages 31-45

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