The role of Rhizobium strains and seed biological priming on germination and seedling growth characteristics of guar (Cyamopsis tetragonoloba L.)

Document Type : Research Paper

Authors

1 Ph.D. Student, Crop Ecology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

2 Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

3 Research Professor Soil and Water Research Institute (SWRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

10.22124/jms.2025.9385

Abstract

In order to investigate the role of Rhizobium strains and biological seed priming on germination and seedling growth traits of guar under laboratory conditions, a factorial study based on a completely randomized design with 3 replications was conducted in 2023 in the Seed Physiology and Biotechnology Laboratory of the Faculty of Agriculture, University of Guilan. The studied factors included 12 treatments: no priming (NoP) + no inoculation (NoI), NoP + inoculation with Mesorhizobium siceri (M.s), NoP + inoculation with Bradyrhizobium japonicum (B.j), NoP + inoculation with Rhizobium leguminosorum (R.l), priming with Bacillus subtilis (B.s) + NoI, priming with B.s + inoculation with M.s, priming with B.s + inoculation with B.j, priming with B.s + inoculation with R.l, priming with Pseudomonas fluorescent (P.f) + NoI, priming with P.f + inoculation with M.s, priming with P.f + inoculation with B.j, priming with P.f + inoculation with R.l. The results showed that priming with P.f+ inoculation with R.l significantly increased most of the desired indicators, including percentage, average time required and germination energy, seedling vigor weight index, tissue water content, etc. compared to the control, which indicates a positive symbiotic relationship between guar and these bacteria and is due to the effect on increasing water absorption, increasing the speed of chemical reactions related to germination, use of seed reserves during germination and early seedling growth. Therefore, the primary metabolites required for seed germination and further growth are provided more quickly and can potentially lead to improved yield in field designs.

Keywords

References

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Iranian Journal of Seed Sciences and Research
Volume 12, Issue 2
July 2025
Pages 33-49

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