Evaluation of improving Sunn hemp (Crotalaria juncea) seed germination with titanium dioxide (TiO2) nanoparticles under salinity stress

Document Type : Research Paper

Authors

1 Ph.D Graduated, Crop Physiology, Department of Plant Production and Genetics, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran

2 Professor, Crop Physiology, Department of Plant Production and Genetics, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran

10.22124/jms.2024.8039

Abstract

Salinity is one of the limiting stresses on agricultural production. The high potential of Sunn hemp (Crotalaria juncea) as a tropical and semi-tropical plant and its scientific and industrial uses caused to investigate its germination potential under saline condition and improving it by titanium dioxide nanoparticles. An experiment conducted in 2023 in the Faculty of Agriculture and Natural Resources of Mohaghegh Ardabili University as factorial based on randomized complete design with three replications. Experimental treatments included different concentrations of seed priming with titanium dioxide nanoparticles (0, 0.5, and 1mM) and salinity stress (0, -0.4, -0.8, and -1.2 MPa) using sodium chloride. The results indicated that the osmotic potentials of -0.4, -0.8, and -1.2 MPa significantly reduced the germination percentage (10.56, 20.49, and 31.26%), radicle length (68.00, 88.52, and91.24%), and plumule length (46.92, 82.59, and 89.87%). Nano priming of seeds by 1 mM increased radicle (25.40%) and plumule length (24.87%) compared to no priming. Also, nano priming enhanced catalase activity by 15.92, 10.18, and 1.35% and proline content by 17.81, 6.26, and 8.40% at osmotic potentials of -0.4, -0.8 and -1.2 MPa. Nano priming at a concentration of 1 mM decreased lipid peroxidation by 30.92, 41.59, and 36.74% at -0.4, -0.8 and -1.2 MPa osmotic potentials. The results of this study, in addition to reporting the germination ability of Sunn hemp seeds under salinity stress of1.2 MPa, emphasized the improvement of germination and related indices by using seed priming with titanium dioxide nanoparticles at a 1mM.

Keywords

References

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Iranian Journal of Seed Sciences and Research
Volume 11, Issue 1
April 2024
Pages 69-87

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